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진주조 마이토콘드리아의 새로운 효소에 의해 유기된 pBR 322 DNA 의 foldback intercoil 구조
김병동,임용표 ( Byung Dong Kim,Yong Pyo Lim ) 생화학분자생물학회 1989 BMB Reports Vol.22 No.1
The foldback intercoil structure of pBR322 DNA was induced by an enzyme which we have recently isolated from pearl millet mitochondria. The enzymatic formation of the unique configurations, namely, the lasso, the stem, the loop, and the stem-and-loop on both the linear and the open circle pBR322 DNA effectively argues that the same configurations found earlier in the native pearl millet mtDNA were not artifacts. Most important, it was demonstrated that the intercoil DNA and the supercoil DNA can be resolved by the electron microscopy. We propose to call the pearl millet mitochondrial protein, $quot;the synaptase$quot;, since it induces intra- and inter-molecular synapsis of two double helical DNA strands.
고추로부터 살리실산에 의하여 유도발현되는 두 가지 유전자의 분리 및 기능분석
김병동 서울대학교 농업개발연구소 1999 농업생명과학연구 Vol.3 No.-
Two salicylic acid (SA)-inducible genes were isolated to investigate the defense mechanism against pathogen in hot pepper plant by mRNA differential display technique. The first SA-inducible cDNA clone, CaSIG4, encoded putative coenzyme A synthetase with a high sequence similarity to the family of AMP-binding proteins. Northern and subcellular localization analyses suggested that CaSIG4 expressed by plant pathogen functions as a probably plasma membrane-bound protein related to fatty acid metabolism. The second SA-inducible cDNA clone, CaSIG19, had significant homology with NAC (NAM, ATAF1/2 and CUC2) domain-containing proteins. A fusion protein of CaSIG19 with GUS was targeted to nucleus of onion epidermal cell upon transient expression. A random oligomer selection experiment with recombinant CaSIG19 protein revealed the possible consensus binding motif as GTFT. CaSIG19 also exhibited transcriptional activation activity in yeast when the protein was fused with LexA DNA-binding domain. Antisense inhibition of CaSIG19 mRNA expression in tobacco plants resulted in spreading of necrotic lesions instead of discrete limiting necrotic lesions upon tobacco mosaic virus infection. These results imply that CaSIG19 is a novel transcription factor and may be involved in plant defense response against pathogen infection.
김병동 서울대학교 농업생명과학대학 농업개발연구소 1998 농업생명과학연구 Vol.2 No.-
We have been conducting a genome research of hot pepper since 1996 as a comprehensive group effort. The objective of our research were: (1) to construct molecular linkage map based on RFLP and AFLP, (2) to select DNA markers tightly linked to major important traits, (3) to breed genetic materials for genome research, and finally (4) to isolate genes controlling major traits through map-based cloning. We constructed a molecular linkage map of pepper using F2 population of a cross between Capsicum annuum (TF68) and Capsicum chinense (Habanero). The map currently contains a total 374 markers, including 134 RFLP markers and 240 AFLP markers. The combined map covered 2,434.4 cM with an average interval size between markers of 6.76 cM.