http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
한국산 고등 균류의 성분 연구 ( Ⅵ ) Amanita Spissaceea 및 Amanita Vaginata 의 아미노산
김병각,이영수,최응칠,심미자,이영남 ( Byong Kak Kim,Young Soo Lee,Eung Chil Choi,Mi Ja Shim,Young Nam Lee ) 생화학분자생물학회 1977 BMB Reports Vol.10 No.1
Amanita spissacea which is indiginous to Korea and Japan and Amanita vaginata which is an edible mushroom, grow wildly in Korea. However, their constituents have never been studied. These higher fungi were collected in the Gyeong Gi Province, Korea, and analyzed for their free and total amino acids, using gas chromatography and amino acid auto-analyzer. Fourteen free amino acids (alanine, glycine, valine, leucine, isoleucine, threonine, aspartic acid, methionine, phenylalanine, histidine, tyrosine, lysine, arginine, and tryptophan) were identified in both species. The contents of eight free essential amino acids in A. spissacea and a. vaginata were : threonine 7.26 and 3.15 ㎎/g, lysine 2. 85 and 2.5 ㎎/g, methionine 2.48 and 2.70 ㎎/g, phenylalanine 1.35 and 0.98 ㎎/g, isoleucine and leucine combined 1.65 and 1.05 ㎎/g, valine 1.24 and 0.68 ㎎/g, and tryptophan 0.90 and 0.93 ㎎/g, respectively. The total contents of the respective amino acids in A. spissacea and A, vaginata were: lysine 5.18 and 12.28 ㎎/g, histidine 3.00 and 5.12 ㎎/g, arginine 7.76 and 9.80㎎/g, threonine 8.88 and 8.00 ㎎/g, glutamic acid 20.10 and 34.94 ㎎/g, alanine 10.22 and 11.62 ㎎/g, valine 9.64 and 8.94 ㎎/g, glycine 8.32 and 8.30 ㎎/g, methionine 1.90 and 1. 63 ㎎/g, isoleucine 6.66 and 7.88 ㎎/g, leucine 12.74 and 14.00 ㎎/g, phenylalanine 7.00 and 7.82 ㎎/g, cysteine 0.82 and 1.26 ㎎/g, aspantic acid 16.94 and 15.28 ㎎/g, serine 9.84 and 10.34 ㎎/g and threonine 3.62 and 3.90 ㎎/g, respectively.
한국산 고등 균류의 성분 연구 (9) : 양송이 버섯의 지방산 성분 Fatty Acids from Agaricus bisporus
김병각,심미자,이만동 한국균학회 1978 韓國菌學會誌 Vol.6 No.1
Attempts were made to investigate on the fatty acids of Agaricus bisporus a cultivated edible mushroom. Fats were extracted from 'it and saponified with alcoholic potassium hydroxide. Isolated fatty acids were methylated and were subjected to column chromatography and G.L.C. Eleven saturated fatty acids, i.e., dodecanoic, tridecanoic, tetradecanoic, pentadecanoic, hexadecanoic, heptadecanoic, octadecanoic, eicosenoic, uncosenoic, docosenoic, and tricosenoic acids, were identified. Especially palmitic and stearic acids were abundant. Nine unsaturated fatty acids appear to exist in the carpophore.
김병각,조덕현,이지열 한국균학회 1978 韓國菌學會誌 Vol.6 No.1
Some 50 fungi were collected and identified at Youngu and Mt. Sobaek from April to October in 1977. The results have shown that, following ten species are new to Korean list. Collybia dryophila (BULL. ex FR.), QUEL., Mycena polygramma (BULL. ex FR.) S.F. GRAY., M. pura (PERS. ex FR.) QUEL., Crepidotus nephrodes (BERK. et CURT.) SACC., Inocybe montana KOBAY., I. squamulosa KOBAY., Rhodophllus ater HONGO, R. violaceus (MURR.) SING., Leccinum aurantiacum (BULL.) S.F. GRAY., and Suillus aeruginascens (SECR.) SNELL. The authors wish to express our thanks to Asan Foundation for the financial support for a part of this research project.
12. 한국산 고등균류의 성분에 관한 연구 (34) : 붉은 싸리버섯의 항암 성분 Antitumor Components of Ramaria formosa (Fr.) Quel
김병각,최응칠,이경림,홍화봉,유인숙 한국균학회 1982 韓國菌學會誌 Vol.10 No.1
To investigate the antitumor components of Korean higher fungi, the carpophores of Ramaria formosa (Fr.) Que´l were collected in Gang Won Province and extracted with hot water. The extracts were concentrated and precipitated by four volumes of enthanol. The precipitates were centrifugated and purified lay dialyzing through visking tube and polysaccharide fractions were obtained. The antitumor activity of the fraction was tested against Sarcoma 180 implanted in mice. The tumor inhibition ratio of the polysaccharide fraction against the tumor was 66% in the dose of 50㎎/㎏/day for the period of tert days. The tumor in two of the ten mice was completely regressed. The components of these aqueous extracts were found to be polysaccharide and protein by color reactions including Anthrone and Lowry-Folin tests. The chemical analysis of the polysaccharide fraction was also undertaken.
김병각,최응칠,김경제,이승희 한국균학회 1985 韓國菌學會誌 Vol.13 No.2
Of 450 Strains isolated from the soil microbes collected in various locations in Korea, a strain had a strong inhibitory activity against bacterial α-amylase and was named strain DMC-72 of the genus Streptomyces. The amylase-inhibitory metabolite produced by this strain was purified by means of acetone precipitation, adsorption on Amberlite IRC-50 and XAD-8, and column chromatography on Amberlite CG-50 and SP-sephadex C-25. The inhibitor which was stable at the pH range of 1∼13 and at 100℃ for half an hour also inhibited other amylases such as salivary α-amylase, pancreatic α-amylase, fungal α-amylase and glucoamylase, but it showed no inhibitory activity against α-glucosidase, β-glucosidase, dextranase and β-amylase. The kinetic studies of the inhibitor showed that its inhibitory effects on starch hydrolysis by α-amylase were non-competitive.
김병각,최응칠,김광욱,김경제 한국균학회 1983 韓國菌學會誌 Vol.11 No.4
To find antimicrobial strains of the soil microorganisms in Korea, they were isolated from the soil samples of different locations and screened for antiobiotic activity against several standard microbes. An isolate among them had an antibacterial activity against grampositive bacteria. The examination of its morphological and biochemical characteristics according to the International Streptnmyces Project methods showed that it belongs to the genus Streptomyces. The strain appears to be a new strain when it was compared with the species within the genus which have been so far reported. The antibiotic metabolite was produced in submerged culture method. Attempts were made to characterize the chemical properties of the antibiotic metabolite.
한국산 고등 균류의 성분 연구(VI) Amanita spissacea 및 Amanita vaginata의 아미노산
김병각,이영수,최응칠,심미자,이영남,Kim, Byong-Kak,Lee, Young-Soo,Choi, Eung-Chil,Shim, Mi-Ja,Lee, Young-Nam 생화학분자생물학회 1977 한국생화학회지 Vol.10 No.1
저자들은 Amanitaceae에 속하는 버섯의 일종인 Amanita spissacea와 Amanita vaginata의 형태학적 특징 및 이들 버섯중에 함유되어 있는 아미노산 분석을 시행하였다. gas chromatography를 이용하여 14종의 유리 아미노산을 확인하였고 8종의 유리 필수 아미노산을 정량하였다. 그리고 이미노산 자동분석기를 이용하여 이들 버섯의 산 가수분해물에 대해 총 아미노산 분석을 시행하였다. 그 결과 Amanita spissacea와 Amanita vaginata에서 14종의 유리 아미노산을 확인하였고 또한 8종의 유리 필수 아미노산중 둘 다 threonine이 가장 많아서 각각 7.3mg/g, 3.2mg/g이었고 총 아미노산 분석 결과 16종의 아마노산을 확인하였고 총 함량은 각각 138mg/g 및 166mg/g이었다. Amanita spissacea which is indiginous to Korea and Japan and Amanita vaginata which is an edible mushroom, grow wildly in Korea. However, their constituents have never been studied. These higher fungi were collected in the Gyeong Gi Province, Korea, and analyzed for their free and total amino acids, using gas chromatography and amino acid auto-analyzer. Fourteen free amino acids (alanine, glycine, valine, leucine, isoleucine, threonine, aspartic acid, methionine, phenylalanine, histidine, tyrosine, lysine, arginine, and tryptophan) were identified in both species. The contents of eight free essential amino acids in A. spissacea and a. vaginata were : threonine 7.26 and 3.15 mg/g, lysine 2.85 and 2.5 mg /g, methionine 2.48 and 2.70 mg/g, phenylalanine 1.35 and 0.98 mg/g, isoleucine and leucine combined 1.65 and 1.05 mg/g, valine 1.24 and 0.68 mg/g, and tryptophan 0.90 and 0.93 mg/g, respectively. The total contents of the respective amino acids in A. spissacea and A. vaginata were: lysine 5.18 and 12.28 rngj'g, histidine 3.00 and 5.12 mg/g, arginine 7.76 and 9.80mg/g, threonine 8.88 and 8.00 mg/g, glutamic acid 20.10 and 34.94 mg/g, alanine 10.22 and 11.62 mg/g, valine 9.64 and 8.94 mg /g, glycine 8.32 and 8.30 mg/g, methionine 1.90 and 1.63 mg/g, isoleucine 6.66 and 7.88 mg/g, leucine 12.74 and 14.00 mg/g, phenylalanine 7.00 and 7.82 mg/g, cysteine 0.82 and 1.26 mg/g, aspartic acid 16.94 and 15.28 mg/g, serine 9.84 and 10.34 mg/g and threonine 3.62 and 3.90 mg/g, respectively.
맥각균의 배양에 관한 연구(III) - 발효조에서의 Lysergic Acid 알칼로이드의 생성
김병각,옥선,최응칠,Kim, Byong-Kak,Auck, Sun,Choi, Eung-Chil 생화학분자생물학회 1973 한국생화학회지 Vol.6 No.3
맥각균을 발효조에서 배양할때 라이서직산 알칼로이드의 생성에 미치는 배양조건 및 첨가물질의 영향을 검토하기 위하여 Claviceps paspali Stevens and Hall 31호 균주를 Schwarting 배양액에서 배양하였으며 Tween 80, 분자상의 산소, 디메칠설폭사이도, 비산 나트륨을 각각 첨가했을 경우 및 솔비톨-푸마르산 배양액과 프로펠라형 교반기를 사용했을 경우의 영향을 실험하였다. 알칼로이드 총량의 정량은 Michelon-Kelleher 법을 이용하였다. 실험결과에 의하면 산소, 디메칠성폭사이드, 비산염의 각각 첨가 및 솔비톨-프마르산 배양액의 사용이 알칼로이드 생성을 증가하였다. 그러나 Tween 80의 첨가는 과잉의 포말을 발생시켜 배양액의 손실을 초래하였으며 결국 알칼로이드의 축적이 감소되었다. Ergot fungus was culitvated in stirred fermentors in various modified conditions to investigate their influence on the production of lysergic acid alkaloids. Claviceps paspali Stevens and Hall Isolate No.31 was cultured in mannitol-succinate medium of Schwarting, et al., and such modifications as additions of Tween 80, oxygen gas, dimethylsulfoxide, arsenate and the utilization of sorbitol-fumaric acid medium and propeller-type impellers were respectively examined. Quantitative determination of the total alkaloids was carried out using Michelon-Kelleher method. The results showed that additions of oxygen gas, dimethylsulfoxide, arsenate, and the utilization of sorbitol-fumaric acid medium increased the production of the alkaloids. However, the addition of Tween 80 caused extensive foaming and resulted in decrease of the alkaloid accumulation.