Interaction of a Novel Biotin-tagged Photoaffinity Probe with Its Target Protein, VEGFR-2

김명희,Sun-Young Han,Bum Tae Kim,Yong Ki Min,Seong Hwan Kim* 대한화학회 2006 Bulletin of the Korean Chemical Society Vol.27 No.9

돼지감자로부터 invertase 의 정제 및 특성 연구

김명희,전덕영 ( Myung Hee Kim,Deok Young Jhon ) 생화학분자생물학회 1988 BMB Reports Vol.21 No.4

The invertase (EC 3.2.1.26), which catalyzes the hydrolysis of sucrose, has been purified 71-fold from Jerusalem artichoke tuber. Electrophoresis showed that the native enzyme is consisted of single polypeptide chain of 86,000 daltons. Optimum pH and temperature for the enzyme action were pH 7.0 and 40℃, respectively. The enzyme exhibited high hydrolytic activity to sucrose, but no activity on inulin. The enzyme had an apparent Km of 24.7 mM for sucrose.

이하선에서 발견된 MALT 림프종 1례

김명희,최종중,안홍근,박중수,김연수,Kim, Myeong Hee,Choi, Jong Joong,Ahn, Hong Geun,Park, Joong Su,Kim, Yeon Soo 대한두경부종양학회 2019 대한두경부 종양학회지 Vol.35 No.1

Mucosa-associated lymphoid tissue (MALT) lymphoma has specific clinical and pathologic features. The most common site MALT lymphomas is the stomach; however, it can also occur in other organs, such as the salivary glands. MALT lymphoma is rare, but its prognosis is good. A 32-year-old man visited Konyang university hospital with parotid mass. Superficial partial parotidectomy was performed to exclude lymphoid neoplasms. IgH gene rearrangement analysis of the surgical specimen led to the diagnosis of MALT lymphoma. The patient underwent esophagogastroduodenoscopy, positron emission tomography-computed tomography, and whole-body bone scan. Regional or distant metastasis was not observed on staging workup. The patient underwent postoperative radiation therapy, there has been no recurrence of MALT lymphoma to date. Here, we report this rare case of parotid MALT lymphoma that was treated with surgery and postoperative radiation therapy.

γ-Cyclodextrin Glycosyltransferase 생산균주의 분리 , 동정 및 효소 생산조건

김명희,손천배,임영희,배경숙,오태광 ( Myung Hee Kim,Cheon Bae Sohn,Young Hee Lim,Kyung Sook Bae,Tae Kwang Oh ) 한국응용생명화학회 1997 Applied Biological Chemistry (Appl Biol Chem) Vol.40 No.6

A cyclodextrin glycosyltransferase-producing bacterium was newly isolated from soil using alkaline pH medium containing 1% Na₂CO₃. The isolated strain was identified as Bacillus brevis by morphological and biochemical characteristics, and fatty acid composition and designated Bacillus brevis CD162. The strain showed the best enzyme production of 0.9 unit/㎖ after 96 hrs of culture at 30℃ in a medium of 2.0% soluble starch, 0.75% yeast extract, 0.5% bacto peptone, 0.2% K₂HPO₄ 0.05% MgSO₄·7H₂O, and 1.5% Na₂CO₃ at initial pH 10.2.

비실험자료를 이용한 연구에서 인과적 추론의 강화: 성향점수와 도구변수 방법의 적용

김명희,도영경,Kim, Myoung-Hee,Do, Young-Kyung 대한예방의학회 2007 예방의학회지 Vol.40 No.6

Objectives : This study attempts to show how studies using non-experimental data can strengthen causal inferences by applying propensity score and instrumental variable methods based on the counterfactual framework. For illustrative purposes, we examine the effect of having private health insurance on the probability of experiencing at least one hospital admission in the previous year. Methods : Using data from the 4th wave of the Korea Labor and Income Panel Study, we compared the results obtained using propensity score and instrumental variable methods with those from conventional logistic and linear regression models, respectively. Results : While conventional multiple regression analyses fail to identify the effect, the results estimated using propensity score and instrumental variable methods suggest that having private health insurance has positive and statistically significant effects on hospital admission. Conclusions : This study demonstrates that propensity score and instrumental variable methods provide potentially useful alternatives to conventional regression approaches in making causal inferences using non-experimental data.

초등학교 저학년과 고학년 학생의 식습관 및 기호도 조사 - 대전지역을 중심으로 -

김명희,윤선영,김미원,Kim, Myung Hee,Yoon, Sun Young,Kim, Mi Won 한국식품영양학회 2013 韓國食品營養學會誌 Vol.26 No.4

The purpose of this study is to analyze the differences of food habits and food preferences between lower and higher grade students through the survey of elementary school students in Daejeon four elementary schools located in Jung-Gu and Seo-Gu in Daejeon are selected for this survey randomly and the completion of the questionnaires are according to grade. 1. Practical Analysis of Food Habits: For regular meals, the highest number of subjects skipped breakfast. It is being found that the girl students (81.61%) consumed more cooked rice than the boys (78.85%) and more boys (13.10%) ate bread or milk more than the girls (9.20%). The most important thing for the lower grades students (34.20%) when consuming is the nutritious foods, while the higher grades students (49.67%) consumed the set meals. During consumption, the highest number of the subjects watched TV or read books from time to time. More higher grades students (38.98%) preferred spicy and salty foods than the lower grades (22.09%). 2. Food Preferences: Between rice and noodles, most subjects preferred cooked rice (81.38%), Jajangmyeon (80.80%), Ramyeon (79.43%), and disliked bean-mixed rice (28.39%). For soups, Most students enjoy the Deokkguk. Majority of the subjects prefer meat and fried or steamed foods, and also grilled and pan-fried dishes. For the pan-roasted and fried dishes, most subjects did not enjoy the rhizome fry and sweet-pumpkin fry methods, and for vegetables, they did not like wild vegetable and seasoned cucumber (40.57%) The higher grades students enjoy the vegetables and salads more than the lower grades students. For Kimchi varieties, it is found that subjects like the cabbage Kimchi (69.66%) the most. There is little difference in food preference between the lower and higher grade students, but the rankings of food preferences are different according to various grades.

부비동염(副鼻洞炎)의 근래(近來) 다양한 침구요법(鍼灸療法)에 대한 문헌적(文獻的) 고찰(考察)

김명희,남상수,이재동,최도영,안병철,박동석,이윤호,최용태,Kim Myung-Hee,Nam Sang-Soo,Lee Jae-Dong,Choi Do-Young,Ahn Byoung-Choul,Park Dong-Seok,Lee Yun-Ho,Choi Yong-Tae 대한침구의학회 1998 대한침구의학회지 Vol.15 No.2

Purpose: This study was carried out to investigate the research of acupuncture and moxibustion treatment for sinusitis. Methods: I mainly investigated 14 different kinds of recent edition of Chinese books and journals as references. Results: In the view of oriental medicine, sinusitis is regarded as Piyon(鼻淵). The loci used in the otopuncture therapies were Naepi(內鼻), Pye(肺), Aeck(額), Shinsangsun(腎上腺), Naebunpi(內分泌), and Weipi(外鼻) according to their frequencies. The drugs used in the drug-injection therapies were Corticoids, Lidocaine, Vitamin B, and herbal drugs. In addition, there were couple therapies mentioned in the references such as the electro-acupuncture therapies, the point penetration therapies, the point through-sinus acupuncture therapies, the point application therapies, the moxibustion therapies, the three-edged needle therapies, the ultra-sono acupuncture therapies, the cupping therapies, and the laser acupuncture therapies. Conclusions: There were various newly developed acupuncture and moxibustion treatment for sinusitis. Among them, the otopuncture therapies, the drug-injection therapies and the electro-acupuncture therapies were more frequently used than others.

운동강도와 지속시간에 따른 트레드밀 운동이 비만 쥐의 심장근 내 항산화에 미치는 영향

김명희,김용억,윤창륙,유지원,안종모,Kim, Myung-Hee,Kim, Young-Eok,Yoon, Chang-Lyuk,Ryu, Ji-Won,Ahn, Jong-Mo 대한안면통증구강내과학회 2013 Journal of Oral Medicine and Pain Vol.38 No.1

트레드밀 운동이 비만 쥐의 심장근 내 항산화에 어떠한 영향을 주는지 알아보고자 흰쥐에 6주 동안 고지방 식이로 비만을 유발시킨 뒤 운동강도와 적용시간을 달리하여 트레드밀을 3주 동안 적용하였으며, 체중변화, 혈중지질성분, 조직학적 변화, 심장근 내 항산화 효소와 MCP-1(monocyte chemoattractant protein-1) 등의 변화를 연구하였다. 4주령 Sprague Dawley계 수컷 흰쥐 32마리를 사용하여 각각 일반사료를 섭취한 정상군 (normal), 비만 대조군 (control), 비만 쥐에게 고강도 분할운동을 적용한 실험군 I(Experimental I), 비만 쥐에게 중강도 지구력 운동을 적용한 실험군 II (Experimental II)로 나누었다. 실험 결과 첫째, 체중변화는 고강도 분할 운동을 적용한 실험군 I과 중강도 지구력 운동을 적용한 실험군 II는 대조군에 비해 유의한 감소가 있었다 (p<0.001). 특히, 운동을 적용한 1주에서 3주까지 유의한 차이가 있었다. 둘째, 혈중지질성분 수치는 고강도 분할 운동을 적용한 실험군 I과 중강도 지구력 운동을 적용한 실험군 II는 대조군에 비해 총콜레스테롤, 중성지방, 저밀도 지단백질의 유의한 감소, 고밀도 지단백질에서는 유의한 증가가 있었다 (p<0.001). 셋째, 심장근의 조직학적 소견은 심장근에서 아교질 형성과 평활근 섬유의 정렬 상태와 밀집도, 심장근 세포크기가 중강도 지구력 운동 적용한 실험군 II에서 정상군과 비슷한 형태인 근육들이 잘 밀집해 있는 것을 볼 수 있었다. 또한, 섬유아세포, 염증세포들의 침윤 등이 고지방 식이 유발 쥐인 대조군과 고강도 분할 운동을 적용한 실험군 I에서 가장 심하였으며, 중강도 지구력 운동을 적용한 실험군 II는 대조군에 비해 섬유아세포, 염증세포들의 침윤 등이 현저히 감소하였다. 넷째, 심장근의 항산화 효소 SOD(superoxide dismutase), CAT(catalase), GPx(glutathione peroxidase) 수치는 중강도 지구력 운동을 적용한 실험군 II와 고강도 분할 운동을 적용한 실험군 I에서 모두 유의한 증가가 있었다 (p<0.001). 다섯째, 심장근의 항산화 단백질 MCP-1 발현 수치는 고강도 분할 운동을 적용한 실험군 I과 중강도 지구력 운동을 적용한 실험군 II 모두에서 감소가 있었으며 (p<0.01), 특히, 중강도 지구력 운동을 적용한 실험군 II에서는 유의한 감소가 있었다 (p<0.001). 따라서, 중강도 지구력 운동은 고지방 식이로 유발된 비만 쥐의 심장근 내 항산화에 영향을 주어 비만 개선과 산화적 스트레스에 의한 심장근육 손상 감소 등에 효과적인 것으로 나타났다. The aims of this study was to observed an effect of antioxidative in cardiac muscle of high fat diet induced obesity rat by treadmill exercise with intensity and time. Thirty-two Sprauge-Dawley rats which were divided into four group. Normal, Control(high fat diet induced obesity rat), Experimental I(high intensity intermittent exercise in high fat diet induced obesity rat), Experimental II(moderate intensity endurance exercise in high fat diet induced obesity rat). The results of this study were as follows: 1. In change of body weight, the outcome of each group significantly difference compared with control. Also, 1 to 3 weeks significantly different compared with pre valu experimental I and II(p<0.001). 2. In change of lipid profile, the outcome of each group significantly difference compared with control(p<0.001). Difference between experimental I and II is not significantly. 3. In change of antioxidative enzymes(SOD, CAT, GPx) in myocardium, there are significant difference between control and experimental II, and also between control and experimental I(p<0.001). 4. In change of antioxidative protein MCR-1, the outcome of each group significantly difference compared with control(p<0.01). Experimental II was most significantly difference than the other group(p<0.001). The above results suggest that treadmill exercise effectively reduced in fat. It would be considered that moderate intensity endurance exercise has an effects on improved antioxidative enzyme in cardiac muscle of high fat diet induced obesity rat.

Rhizopus nigricans 에 의한 Progesterone 의 Hydroxylation (1) : 반응 조건의 영향

김명희,김말남 ( Myung Hee Kim,Mal Nam Kim ) 한국균학회 1987 韓國菌學會誌 Vol.15 No.1

요양병원 입원 노인의 우울 관련요인

김명희,김윤경,Kim, Myung-Hee,Kim, Yun-Kyung 대한예방한의학회 2012 대한예방한의학회지 Vol.16 No.3

Objective : The study was designed to identify the factors related to depression of the elderly patients in geriatric hospital. Method : The subjects consisted of 198 old people who admitted in three geriatric hospital located in Daegu City. The Data was collected through interview with questionnaire from July 16 to August 16, 2012. Results : The general characteristics which significantly affected depression was perceived health status(F=18.158, p=.000). There was a slightly negative correlation between activity daily living & depression(r=-.378,p=.000) and strongly negative correlation between life satisfaction & depression(r=-.573, p=.000). Among the factors related to depression of the subjects, life satisfaction had highest expanatory power of 32.8%. Conclusion : The finding of this study provide useful information for constructing an intervention program and care for the elderly patients in geriatric hospital with depression.