해양 홍조류 Laurencia sp. (Ceramiales: Rhodomelaceae)에서 분리한 Oceanisphaera sp. JJM57의 분리 및 동정

김만철,문영건,김동휘,손홍주,허문수,Kim, Man-Chul,Dharaneedharan, S.,Moon, Young-Gun,Kim, Dong-Hwi,Son, Hong-Joo,Heo, Moon-Soo The Microbiological Society of Korea 2013 미생물학회지 Vol.49 No.1

본 연구는 한국 제주도 조간대에 서식하는 홍조류로부터 분리된 JIM57 균주의 계통학적 특성을 조사하기 위하여 수행되었다. 16S rRNA gene 염기서열을 분석한 결과, 본 균주는 Oceanisphaera 속과 대단히 유사하였으며, Oceanisphaera litoralis DSM $15406^T$와 98.02%, O. donghaessis KCTC $12522^T$와 97.7%의 염기서열 상동성을 나타내었다. 본 균주는 그람양성의 호기성 구균으로써, 0.5-8.0%의 NaCl 및 $4-47^{\circ}C$에서 생육할 수 있었다. 본 균주는 Oceanisphaera litoralis DSM $15406^T$와 일부 생리학적 및 생화학적 특성을 공유하였으나 ethanol, proline 및 alanine 이용성에서는 차이가 있었다. 본 균주 genomic DNA의 GC 함량은 61.94 mol%였으며, 주요 균체 지방산 지방산으로서 $C_{16:1}$ ${\omega}7c$, iso-$C_{15:0}$ 2-OH, $C_{16:0}$, and $C_{18:1}$ ${\omega}7c$를 함유하고 있었다. 또한 DNA-DNA 상동성을 조사한 결과, JIM57 균주는 O.litoralis DSM $15406^T$ 및 O. donghaessis KCTC $12522^T$와 별개의 종임을 알 수 있었다. 이러한 결과들을 종합한 결과, JIM57 균주(=KCTC 22371 =AM 983543 =CCUG 60764)는 O. litoralis DSM $15406^T$ 및 O. donghaessis KCTC $12522^T$와 다른 특성을 나타내는 것으로 확인되어 Oceanisphaera의 새로운 종임을 제안하였다. A taxonomic study was carried out to assess the phylogenetic characteristics of isolate JJM57 from marine red algae Laurencia sp. collected from intertidal zone in Jeju Island, South Korea. Comparative analysis of 16S rRNA gene sequence shows that this isolate belongs to the genus Oceanisphaera. It shows 98.02% and 97.7% sequence similarity with Oceanisphera litoralis DSM $15406^T$ and Oceanisphera donghaensis KCTC $12522^T$, respectively. Strain JJM57 is a Gram-negative, aerobic, moderately halophilic bacterium able to grow in different NaCl concentration ranges from 0.5 to 8.0% and at varying temperatures from 4 to $37^{\circ}C$. Sharing some of the physiological and biochemical properties with O. litoralis and O. donghaensis, JJM57 strain differs in the utilization of ethanol, proline, and alanine. The G+C contents of the strain JJM57 is 61.94 mol% and it is rich in $C_{16:1}$ ${\omega}7c$ and/or iso-$C_{15:0}$ 2-OH, $C_{16:0}$, and $C_{18:1}$ ${\omega}7c$ fatty acids. The DNA-DNA relatedness data separates the strain JJM57 from other species such as O. litoralis and O. donghaensis. On the basis of these polyphasic evidences, present study proposed that strain JJM57 (=KCTC 22371 =AM983543 =CCUG 60764) represents a novel bacterial species of Oceanisphaera.

POSSIBILITIES AND LIMITATIONS OF APPLYING SOFTWARE RELIABILITY GROWTH MODELS TO SAFETYCRITICAL SOFTWARE

김만철 한국원자력학회 2007 Nuclear Engineering and Technology Vol.39 No.2

It is generally known that software reliability growth models such as the Jelinski-Moranda model and the Goel- Okumoto’s non-homogeneous Poisson process (NHPP) model cannot be applied to safety-critical software due to a lack of software failure data. In this paper, by applying two of the most widely known software reliability growth models to sample software failure data, we demonstrate the possibility of using the software reliability growth models to prove the high reliability of safety-critical software. The high sensitivity of a piece of software’s reliability to software failure data, as well as a lack of sufficient software failure data, is also identified as a possible limitation when applying the software reliability growth models to safety-critical software.

Evaluation of Effectiveness of Fault-Tolerant Techniques in a Digital Instrumentation and Control System with a Fault Injection Experiment

김만철,서정일,정원대,최종균,강현국,이승준 한국원자력학회 2019 Nuclear Engineering and Technology Vol.51 No.3

Recently, instrumentation and control (I&C) systems in nuclear power plants have undergone digitalization. Owing to the unique characteristics of digital I&C systems, the reliability analysis of digital systemshas become an important element of probabilistic safety assessment (PSA). In a reliability analysisof digital systems, fault-tolerant techniques and their effectiveness must be considered. A fault injectionexperiment was performed on a safety-critical digital I&C system developed for nuclear power plants toevaluate the effectiveness of fault-tolerant techniques implemented in the target system. A softwareimplementedfault injection in which faults were injected into the memory area was used based onthe assumption that all faults in the target system will be reflected in the faults in the memory. To reducethe number of required fault injection experiments, the memory assigned to the target software wasanalyzed. In addition, to observe the effect of the fault detection coverage of fault-tolerant techniques, aPSA model was developed. The analysis of the experimental result also can be used to identify weakpoints of fault-tolerant techniques for capability improvement of fault-tolerant techniques

일괄입찰방식의 적격심사분이별 배점 결정모델 개발

김만철,구교진,현창택,Kim Man-Chul,Koo Kyo-Jin,Hyun Chang-Taek 한국건설관리학회 2005 건설관리 : 한국건설관리학회 학회지 Vol.6 No.4

건설 전체 프로세스를 하나의 주체가 단일 책임하에 수행하는 일괄입찰방식은 설계와 시공의 연계로 인한 기술력확보, 공기단축, 사업비절감, 품질확보 등의 예상효과로 인해 적용이 확대되리라 예상되는 발주방식의 하나이다. 한편, 국내 일괄입찰방식의 낙찰자 결정방법은 일괄입찰방식으로 수행되는 모든 프로젝트에 동일한 배점기준을 적용하도록 되어 있어, 일괄사업자 선정시 발주자의 목적과 프로젝트의 특성을 반영하기 어려운 것이 현실이다. 이에 본 연구에서는 일괄사업자 선정에 있어서 발주자의 목적과 프로젝트의 특성을 반영할 수 있는 일괄입찰방식의 적격심사 분야별 배점 결정모델을 제안함으로써, 발주자가 당해 프로젝트에 최적인 일괄사업자를 합리적으로 선정하는 것을 돕고자 하였다. Design-build that a single entity performs whole construction process under singular responsibility is one of the project delivery system comes to take expectation effects of time savings, cost saving, and quality assurance. On the other hand, a current domestic method for selecting a design-build contractor is difficult to reflect the purpose of owner and the project characteristics when owner selects the design-build contractor. The purpose of this research is to suggest a model for deciding evaluation weights in design-build which can reflect the purpose of owner and the project characteristics. This research can help owner to select the best suitable design-build contractor for the project.

궤간외측 포인트 가드레일에 의한 차량 주행 특성에 대한 수치해석적 연구

김만철,김은,윤병현,오재훈,송봉환,전기태 한국철도학회 2021 한국철도학회 학술발표대회논문집 Vol.2021 No.6

해양방선균 Streptomyces sp. ACT-1의 동정 및 항산화 활성

김만철,김주상,김윤범,한용재,허문수,Kim, Man-Chul,Kim, Ju-Sang,Kim, Yun-Beom,Harikrishnan, Ramasamy,Han, Yong-Jae,Heo, Moon-Soo 한국미생물학회 2009 미생물학회지 Vol.45 No.4

For the research of the natural antioxidant from marine sources, an antioxidant-producing marine actinomycetes was isolated from sea water in Jeju coastal area. The strain was identified based on 16S rDNA sequencing, the morphology by a method of scanning electron microscopy, physiological and biochemical characteristics and cellular fatty acid analysis. The isolated strain ACT-1 cell size was $0.5\sim1.0{\mu}m$ and gram positive, aerobic, nonmotile, substrate mycelium are red and gray aerial mycelium. 16S rRNA sequence analysis showed that were Gram-positive bacteria grouped on Streptomyces genus. Results of cellular fatty acid analysis showed that major cellular fatty acids were $C_{15:0}$ anteiso (39.33%), $C_{16:1}$ cis 9 (11.96%), $C_{16:0}$ (13.08%) and $C_{17:0}$ anteiso (10.99%). Finally, strain was identified Streptomyces sp. ACT-1. The antioxidant activity of methanol extract from Streptomyces sp. ACT-1 was evaluated by measuring DPPH, hydroxyl, and alkyl radical scavenging activity using an electron spin resonance (ESR) spectrometer. DPPH radical scavenging activity of SBME-1 (Streptomyces broth methanol extract) was 67% at $1,000{\mu}g$/ml. Hydroxyl radical scavenging activity of SBME-1 was 84% at $500{\mu}g$/ml. Alkyl radical scavenging activity of SBME-1 was 71% at $1,000{\mu}g$/ml. 이 연구는 해양 유래의 천연 항산화물질에 관한 연구로서 항산화물질 생산 해양 방선균은 제주연안 해수에서 분리되었다. 균주는 16S rRNA 염기서열, 전자현미경에 의한 형태학적, 생리, 생화학적 및 세포 지방산 분석을 기초로 동정되었다. 분리균주 ACT-1은 그람양성, 호기성, 비운동성, 기질균사체색이 red, 기중균사체 색이 gray계열 이었다. 세포지방산 분석결과 주요 지방산으로 $C_{15:0}$ anteiso (39.33%), $C_{16:1}$ cis 9 (11.96%), $C_{16:0}$ (13.08%)와 $C_{17:0}$ anteiso (10.99%)로 분석되었으며, 최종적으로 Streptomyces sp. ACT-1으로 동정되었다. Streptomyces sp. ACT-1 메탄올 추출물의 항산화활성은 DPPH (1,1-diphenyl-2-picrylhydrazyl), hydroxyl, alkyl radical 소거활성을 ESR (electron spin resonance) spectrometer를 이용하여 측정되었다. SBME-1 (Streptomyces broth methanol extract)의 DPPH 라디컬 소거활성은 $1,000{\mu}g$/ml 농도에서 67%, Hydroxyl radical 소거활성은 $500{\mu}g$/ml에서 84%, Alkyl radical 소거활성은 $1,000{\mu}g$/ml 농도에서 71%를 보였다.

A Quantitative Model of System- Man Interaction Based on Discrete Function Theory

김만철,성풍현 한국원자력학회 2004 Nuclear Engineering and Technology Vol.36 No.5

A quantitative model for a control system that integrates human operators, systems, and their interactions is developed based on discrete functions. After identifying the major entities and the key factors that are important to each entity in the control system, a quantitative analysis to estimate the recovery failure probability from an abnormal state is performed. A numerical analysis based on assumed values of related variables shows that this model produces reasonable results. The concept of ‘relative sensitivity’is introduced to identify the major factors affecting the reliability of the control system. The analysis shows that the hardware factor and the design factor of the instrumentation system have the highest relative sensitivities in this model. The probability of human operators performing incorrect actions, along with factors related to human operators, are also found to have high relative sensitivities. This model is applied to an analysis of the TMI-2 nuclear power plant accident and systematically explains how the accident took place.

분기기 궤간 외측 포인트 가드레일에 현장성능평가

김만철,김은,윤병현,오재훈,송봉환,전기태,황광하 한국철도학회 2021 한국철도학회 학술발표대회논문집 Vol.2021 No.11

VTS 기능비교를 통한 개선 방안 도출

김만철,김봉석,류시량 한국항해항만학회 2012 한국항해항만학회 학술대회논문집 Vol.2012 No.공동학술

글로벌 기업을 향한 KEPCO의 해외사업

김만철 서울행정학회 2011 서울행정학회 학술대회 발표논문집 Vol.2011 No.1