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김두운,Byeong-Mun Jeong,김위식,김종오,Chae Hong Lim,Jeong-Young Cho,은종방,Jae-Hak Moon,김석렬,Myoung-Ae Park,오명주 한국식품과학회 2010 Food Science and Biotechnology Vol.19 No.6
Efficiency of extraction and concentration methods for the detection of the major capsid protein gene of lymphocystis disease virus from different tissues of olive flounders (Paralichthys olivaceus) was tested. Tris elution buffer showed a 100 fold higher polymerase chain reaction (PCR) detection limit than TE elution buffer in the virus extraction step from skin tissues. Using the TRPD (Tris elution buffer, polyethylene, and DNA extraction kit)procedure, we confirmed that skin tissues and lymphocystis cells of olive flounders had a detection limit of 10−6 and 10−7 PCR-U/μL, meaning that 106 and 107 fold dilutions of lymphocystis disease virus (LCDV) were PCR positive,respectively.
김두운,Se-Na Kim,백근식,박성찬,Chae Hong Lim,김종오,신태선,오명주,성치남 한국미생물학회 2011 The journal of microbiology Vol.49 No.1
A metagenomic fosmid library was constructed using genomic DNA isolated from abalone intestine. Screening of a library of 3,840 clones revealed a 36 kb insert of a cellulase positive clone (pAM11E10). A shotgun clone library was constructed using the positive clone (pAM11E10) and further screening of 3,840 shotgun clones with an approximately 5 kb insert size using a Congo red overlay revealed only one cellulase positive clone (pAM11L9). The pAM11L9 consisted of a 5,293-bp DNA sequence and three open reading frames (ORFs). Among the three ORFs, cellulase activity was only shown in the recombinant protein (CelAM11)coded by ORF3, which showed 100% identity with outer membrane protein A from Vibrio alginolyticus 12G01, but no significant sequence homology to known cellulases. The expressed protein (CelAM11) has a molecular weight of approximately 37 kDa and the highest CMC hydrolysis activity was observed at pH 7.0 and 37°C. The carboxymethyl cellulase activity was determined by zymogram active staining and different degraded product profiles for CelAM11 were obtained when cellotetraose and cellopentaose were used as the substrates, while no substrate hydrolysis was observed on oligosaccharides such as cellobiose and cellotriose.
김두운,김석렬,Ki-Sung Kwon,Ji-Won Lee,오명주 한국미생물학회 2008 The journal of microbiology Vol.46 No.4
The molecular methods using polymerase chain reaction have been proposed as useful tools for the identification of viral pathogens in food and water. However, the PCR-based methods are highly dependent on the methods of virus concentration and nucleic acid purification due to the low sensitivity of PCR in the presence of PCR inhibitors. We developed TPTT [tris elution buffer-PEG-TRIzol-poly(dT) magnetic bead] protocol in order to detect hepatitis A virus (HAV) inoculated in oyster digestive glands. The detection limit of HAV precipitated with zirconium hydroxide was 105 fold less sensitive in a nested PCR than that precipitated the HAV supernatant twice with PEG/NaCl (16% polyethylene glycol 6,000, 0.525 M NaCl) in a 1:2 (v/v) ratio, which provided an efficient detection of 0.0148 PFU/g from approximately 0.05 g of oyster homogenate. This method is efficient for potential use in the detection of HAV from shellfish and is more sensitive than most currently published tests.
김두운,Kim, Du-Woon The Korean Society of Food Preservation 2008 한국식품저장유통학회지 Vol.15 No.5
Sequential passes through $Sephadex^{TM}$ columns were used to select phages that displays ligands for dextran ($\alpha$-1,6 linked linear chains) from a phage antibody library. Those phages that bound to the $Sephadex^{TM}$ in each iteration were replicated in E. coli. A phage preparation isolated on the third round selection produced 5.4 nephelos turbidity units (NTU) in a dextran specific immunonephelometric assay, a 2.2 fold higher value than the phage preparation from the first round selection. This phage gave $72\;{\pm}\;10$ normalized intensity (N.I.) in a dip-stick assay against high molecular size dextran (T2000, $2\;{\times}\;10^6) and significantly lower color ($30\;{\pm}\;6$ N.I.) against low molecular size dextran (T10, $10^4$). The presence of an Fab insert in each of these phages was confirmed using a $\beta-galactosidase linked assay and polymerase chain reaction.
Acinetobacter soli sp. nov., Isolated from Forest Soil
김두운,백근식,Mi Sun Kim,박성찬,Seon Suk Kim,이문수,곽영세,성치남 한국미생물학회 2008 The journal of microbiology Vol.46 No.4
A non-motile and rod shaped bacterium, designated strain B1^T, was isolated from forest soil at Mt. Baekwoon, Republic of Korea. Cells were Gram-negative, catalase-positive, and oxidase-negative. The major fatty acids were 9-octadecenoic acid (C_18:1 ω9c; 42%) and hexadecanoic acid (C_16:0; 25.9%) and summed feature 3 (comprising iso-C_15:0 2-OH and/or C_16:1 ω7c; 10.0%). The DNA G+C content was 44.1 mol%. A phylogenetic tree based on 16S rRNA gene sequences showed that strain B1^T formed a lineage within the genus Acinetobacter and was closely related to A. baylyi DSM 14961^T (98.6% sequence similarity), followed by A. baumannii DSM 30007^T (97.4%), A. calcoaceticus DSM 30006^T (97.0%), and 3 genomic species (96.8~ 7.6%). Phenotypic characteristics, gyrB gene sequence analysis and DNA-DNA relatedness data distinguished strain B1T from type strains of A. baylyi, A. baumannii, and A. calcoaceticus. On the basis of the evidence presented in this study, strain B1^T represents a novel species of the genus Acinetobacter, for which the name Acinetobacter soli sp. nov. is proposed. The type strain is B1^T (= KCTC 22184^T= JCM 15062^T).
동적 시뮬레이션에 의한 도시가로경관 관리지표의 허용범위 연구 - 건축물 형태 및 배치를 중심으로 -
김두운,변재상,임승빈,Kim, Doo-Wun,Byeon, Jae-Sang,Im, Seung-Bin 한국조경학회 2008 韓國造景學會誌 Vol.35 No.6
As urban residents' standard of living has recently risen, efforts to improve urban landscapes have increased. It is very important to manage streetscapes to improve the urban landscape because they are one of the essential elements in city construction and urban landscaping. This study focuses on the indicators that manage streetscapes more accurately and realistically. To achieve this purpose, this study used dynamic simulations considering shape and layout of buildings, the primary factors in streetscapes. This study can be summarized as follows: 1. Two indicators to manage streetscape were investigated in previous studies: one to increase visual openness and the other to reduce visual overstimulation. These indicators had high correlation with scenic beauty. Therefore, increasing openness and reducing overstimulation are essential to improve streetscapes. 2. Two household tower type buildings should be arranged along roadsides to increase openness and scenic beauty. However, low tower buildings with two household are not suitable along roadsides because they create monotony and intervals are needed between buildings. 3. To increase openness, the angled arrangement of buildings is suggested $75^{\circ}{\sim}90^{\circ}(105^{\circ}{\sim}135^{\circ})$ for low buildings, $30^{\circ}{\sim}45^{\circ}(135^{\circ}{\sim}150^{\circ})$ for mid-sized buildings, and $75^{\circ}{\sim}90^{\circ}(105^{\circ}{\sim}135^{\circ})$ for high buildings. 4. To reduce overstimulation, the height and setback control regulations should be at an angle of less than $45^{\circ}$. This study suggests more accurate management guidelines by organizing the indicators that could effectively manage streetscapes and by overcoming limitations of reality shown in a static simulation.