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자가면역글로불린 G 측정을 위한 표면탄성파 바이오센서에 대한 연구
김기범 ( Gi Beum Kim ),정우석 ( Woo Suk Cheong ),박영란 ( Young Ran Park ),김상진 ( Shang Jin Kim ),김성종 ( Seong Jong Kim ),강형섭 ( Hyung Suk Kang ),김진상 ( Jin Shang Kim ),홍철운 ( Chul Un Hong ) 한국화학공학회 2011 Korean Chemical Engineering Research(HWAHAK KONGHA Vol.49 No.2
In this study, we have developed shear horizontal(SH) surface acoustic wave(SAW) sensors for the detection of immunoglobulin G(IgG) on the gold coated delay line of SH-SAW devices. As the result of the experiment, we could uniformly immobilize anti-MIgG(mouse IgG) conjugate on the surface of gold. When displaying results of immobilization on the surface of gold using G-anti MIgG conjugate and blocking buffer in frequency shift, G-anti MIgG conjugate showed frequency shift of 75.1 kHz in the initial frequency, and blocking buffer showed frequency shift of 215.7 kHz. When various concentrations of MIgG was added in 100 MHz type sensor, the sensor showed 46.3, 127.45, 161.21 and 262.39 kHz frequency shift at 25, 50, 75 and 100 μg MIgG concentration, respectively.
해양퇴적물에서의 중금속 및 유기화합물 분석법의 원리 및 특징 비교 : 퇴적물 내 HNS 관리대상물질의 새로운 분석법 개발을 위한 기초문헌 연구
김기범(Gi Beum Kim),정해진(Haejin Jeong),장유리(Yu Lee Jang),김나영(Na Yeong Kim),오상우(Sangwoo Oh),이문진(Moonjin Lee) 한국해양환경·에너지학회 2021 한국해양환경·에너지학회 학술대회논문집 Vol.2021 No.10
국내 해양산업시설 HNS 배출에 대한 배출기준 정립을 위해서는 현장 실태조사 및 생태독성 연구 등의 복합적인 다학제 연구가 필요하다. 그 중 실태조사를 위해서는 해양매체인 해수 및 해양퇴적물에서의 관리대상물질에 대한 분석법 정립이 필요하다. 본 연구에서는 〈해양산업시설 배출 위험유해물질 관리기술 개발 사업〉의 일환으로 연구의 최종목표인 관리대상물질의 배출기준을 제시하여 위하여 현장 실태조사를 통한 해수 및 해양퇴적물에서의 정확한 잔류농도를 파악하는 것이 필수적이다. 하지만 이들 물질의 해수 및 퇴적물에서의 잔류현황을 파악하기 위해 새로운 분석법의 개발이 필요하다. 그러므로 2차년도부터 특정 관리대상물질에 대한 분석법을 빠르게 정립하기 위한 기반 연구로서 본 1차년도 연구에서는 해양수산부, 환경부, 미국 환경보호청 및 기존 문헌을 통해 퇴적물에 대한 중금속 및 유기화합물의 다양한 분석법을 정리하였다. 금속류의 경우 퇴적물은 산분해법, 해수에 대해서는 용매 추출법 등이 있다. 퇴적물에 대한 산분해법은 질산법, 질산-염산법, 질산-황산법, 질산-과염소산법, 질산-과염소산-불화수소산법 등이 있으며, 완전추출법 혹은 부분 추출법 등 연구목적에 따라 퇴적물에 대해 적절한 산분해법을 사용한다. 필요에 따라 재추출하거나 희석 등을 실시한다. 국내 공정시험 기준에 제시된 구리, 니켈, 망간, 아연, 주석 등 대표 화합물은 원자흡수 분광광도법, 유도결합 플라스마 원자발광분광법, 유도결합 플라스마 질량분석법 등으로 정량분석한다. 유기화합물의 경우, 속실렛, 진탕추출 등의 방법으로 추출한 뒤, 흡착제를 채운 유리컬럼으로 정제 후 농축한다. 필요에 따라서는 유도체화와 용매치환 등을 실시한다. 최종적으로 기체/액체 크로마토그래피로 물질을 분리한 후 질량분석 검출기, 불꽃 이온화 검출기 또는 광이온화 검출기 등 다양한 검출기로 정성정량 분석한다. 1차년도 연구를 통해 퇴적물 중 중금속 및 유기화합물에 대한 다양한 분석법을 통해 추출, 정제, 농축, 기기분석 등 각 분석 단계별로 공통점 및 차이점을 요약하였고, 차이점에 대해서는 그 이유를 파악하였다. 이러한 연구를 통해 2차년도부터 해양환경공정시험기준에 포함되지 않은 특정 중금속 및 유기화합물의 분석법을 개발하는데 있어서 기반을 구축할 수 있게 되었다. In order to establish the guideline for HNS emission from marine industrial facilities, complex multidisciplinary studies such as field surveys and ecotoxicity studies are needed. Among them, it is necessary to establish new analytical method of the substances to be managed in seawater and marine sediments for the field survey. In this study, the final goal of the 〈Development of Technology for Impact Assessment and Management of HNS discharged from Marine Industrial Facilities〉 was to present the guideline of substances to be managed, for which it is essential to identify the exact residual concentrations in seawater and marine sediments through field surveys. If there is not an appropriate method for the substances in Korean Standard Method of Examination for Marine Environment, it is necessary to develop a new analytical method to determine these substances in seawater and sediments. Therefore, in this first year, the analytical methods for the heavy metals and organic compounds on sediments through the Ministry of Oceans and Fisheries, the Ministry of Environment, the United States Environmental Protection Agency, and the scientific papers were reviewed. For metals, there are acid extraction methods for sediments, and solvent extraction methods for seawater. Solvents for acid extraction methods include nitric acid, the mixture of nitric acid-hydrochloric acid, the mixture of nitric acid-sulfuric acid, the mixture of nitric acid-perchloric acid, the mixture of nitric acid-perchloric acid-hydrofluoric acid, etc. Complete extraction method or partial extraction method was used according to the research purpose. If necessary, 2<SUP>nd</SUP> extraction or additional dilution was performed. Representative metals such as copper, nickel, manganese, zinc, and tin are quantitatively analyzed by atomic absorption spectroscopy, inductively coupled plasma atomic emission spectroscopy, and inductively coupled plasma mass spectrometry. After extraction by Soxhlet and shaking extraction, organic compounds were purified with a glass column filled with an adsorbent and then concentrated. If necessary, derivatization and solvent replacement are performed. Finally, the organic compounds are separated by gas/liquid chromatography, and then the qualitative and quantitative analysis is performed with various detectors such as mass spectrometry detector, flame ionization detector, or photoionization detector. Through the first year study, the similarities and differences were summarized for each analysis step, such as extraction, purification, concentration, and instrumental analysis for heavy metals and organic compounds in the sediment. The reasons for the differences were identified too. Through these studies, from the second year, it will be possible to develope new analytical methods for specific heavy metals and organic compounds that were not included in Korean Standard Method of Examination for Marine Environment.
인공 폐 보조장치 내에서의 유체 유동 모델링에 대한 연구
김기범 ( Gi Beum Kim ),박영란 ( Young Ran Park ),김상진 ( Shang Jin Kim ),홍철운 ( Chul Un Hong ),강형섭 ( Hyung Sub Kang ),김진상 ( Jin Shang Kin ),김성종 ( Seong Jong Kim ),김민호 ( Min Ho Kim ) 한국화학공학회 2011 Korean Chemical Engineering Research(HWAHAK KONGHA Vol.49 No.2
In this study, the characteristic of fluid flow in the new type lung assist devices has been established using computational fluid dynamics(CFD). For the modeling, the hollow fiber was ignored, and vertical types and tangential types were used for the model. Which was to analyze the flow characteristics of the fluid Dow model when there exists 1 and 2 input/output pods, and when the input/output ports is located at the center of the cylinder and at the tangential direction with the cylinder wall. The modeling results showed that it was possible to eliminate no-flow region(stagnant layer) as shown in the vertical type when an inlet and an outlet were installed on the tangential direction of the cylinder as shown in the tangential type. Also, in the tangential type, vortex-type flow appeared as dominant, and it showed a complicated flow not deviated to one side. When the number of input/output was two, there was no deviated flow, and complicated flows were generated all across the tube. From the test result, it was found that input/output of flow was tangential type and complicated flows with no stagnant layer would be generated when there are two inputs/outputs, respectively.
Microencapsulation 헤모글로빈 인공적혈구의 용혈
김민호 ( Min Ho Kim ),강형섭 ( Hyung Sub Kang ),김진상 ( Jin Shang Kim ),백병걸 ( Byeong Kirl Baek ),홍철운 ( Chul Un Hong ),김성종 ( Seong Jong Kim ),신형식 ( Hyung Shik Shin ),김기범 ( Gi Beum Kim ) 한국조직공학과 재생의학회 2010 조직공학과 재생의학 Vol.7 No.2
The purpose of this study was to reduce the hemolysis of artificial red blood cells by extracting phospolipids from egg yolk through microencapsulation to evaluate the oxygen transfer rate of artificial red blood cells in an intravascular artificial lung device. In this study, hemosome was produced to encapsulate hemoglobin which extracted the red blood cell of bovine blood with phospolipids reduced egg yolk. The solution blended blood with hemosome at the rate of 4 to 1 and normal blood measured the oxygen transfer rate. The hemolysis of the microencapsulated artificial red blood cell measured less than the normal red blood cell through absorptivity. Due to the phospolipids enclosing the red blood cell to protect it, the produced hemosome operated at a higher oxygen transfer rate by reducing the demolition of hemoglobin. Consequently, hemolysis was observed to advance through micoencapsulation.
CFD 해석을 이용한 Balloon형 인공심폐기 설계를 위한 구조적 해석
박영란 ( Young Ran Park ),심정연 ( Jeong Yeon Shim ),김기범 ( Gi Beum Kim ),김상진 ( Shang Jin Kim ),강형섭 ( Hyung Sub Kang ),김진상 ( Jin Shang Kim ),김민호 ( Min Ho Kim ),홍철운 ( Chul Un Hong ),김성종 ( Seong Jong Kim ) 한국화학공학회 2011 Korean Chemical Engineering Research(HWAHAK KONGHA Vol.49 No.2
In this study, we attempted a structural analysis in order to design a balloon type extracorporeal membrane oxygenator that can induce blood flow without using blood pumps for the purpose of complementing the weakness in the existing extracorporeal membrane oxygenator. To analyze the flow characteristic of the blood flow within the virtual model of extracorporeal membrane oxygenator, computational fluid dynamics(CFD) modeling method was used. The operating principle of this system is to make the surface of the extracorporeal membrane oxygenator keep contracting and dilating regularly by applying pressure load using a balloon, and the ``Time Function Value`` that changes according to the time was applied by calculating a half cycle of sine waveform and a cycle of sine. waveform Under the assumption that the uni-directional blood flow could be induced if the balloon type extracorporeal membrane oxygenator was designed as per the method described above, we conducted a structural analysis accordingly. We measured and analyzed the velocity and pressure of blood flow at both inlet and outlet of the extracorporeal membrane oxygenator through CFD simulation. As a result of the modeling, it was confirmed that there was a flow in accord with the direction of the blood by the contraction/dilation. With CFD simulation, the characteristics of blood flow can be predicted in advance, so it is judged that this will be able to provide the most optimized design in producing an extracorporeal membrane oxygenator.
정우석,김기범,홍철운,Chong, Woo-Suk,Kim, Gi-Beum,Hong, Chul-Un 한국전기전자재료학회 2005 전기전자재료학회논문지 Vol.18 No.11
The purpose of this study is to obtain strong bond strength at the interface between piezoelectric substrates and semiconductor thin films to be applied for the manufacture of high-performance acoustic wave semiconductor coupled device. For this purpose, we have compared and examined the effects of different surface treatment methods on hydrophile properties at the surface of the piezoelectric substrates. Moreover, we have observed the effect of microwave and laser on the elimination of water molecules at the interface. As for the piezoelectric substrates, dry method for surface treatment was found to be superior in the control of hydrophilicity of the surface compared to wet method. On the other hand, both microwave and laser were found to be effective in the elimination of water molecules in the interface.
양영석,김기범,Yang, Yeong-Seok,Kim, Gi-Beum 대한의용생체공학회 2019 의공학회지 Vol.40 No.3
The purpose of this study is to evaluate the effects of ultraviolet blood irradiation on the blood when a low dose of ultraviolet (UV) C is directly irradiated to the blood in a diabetic rabbit model and to evaluate the effects on treatment for diabetes. This study results indicate that the reduced body weight is increased and blood glucose levels are significantly reduced after the UBI treatment is performed when compared to those prior to the UBI treatment. In addition, $HCO_3{^-}$ levels and blood pH were elevated and lowered, respectively. When the UBI treatment is performed in a diabetic rabbit model, in this result indicate that blood glucose levels are reduced.