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쥐의 Natural Killer Cell Assay의 표적세포로 이용되는 Yac-1세포의 염색체에 관한 연구
김강련 고신대학교(의대) 고신대학교 의과대학 학술지 1986 고신대학교 의과대학 학술지 Vol.2 No.1
The metaphase chromosomes of the Yac-1 cells stained with trypsingiemsa method were observed and the results obtained are summarized as fellowing, 1) The basic karyothype of the Yac-1 cells was consisted of 40 chromosomes. 2) The chromosome types classified according to the position of centromere were acrocentric to telocentric in 20 parts of the whole. 3) The staiing intensity of the band recorded in four degrees were, very dark in 23, medium dark 19, weakly positive in 16, pale in 38 bands. And the total number of bands was recorded as 96 in 20 pairs of the whole.
비치명적 만성 저산소증에서 뇌의 신경 혈관계의 적응 : Neurotrophic factor의 역할 Roles of neurotrophic factor
김강련 KOSIN UNIVERSITY COLLEGE OF MEDICINE 2006 高神大學校 醫學部 論文集 Vol.21 No.1
Hypoxia episodes of prenatal and perinatal brain are causes of significant long-term neurologic morbidity, mental retardation, seizures, and cerebral palsy, owing to the neuronal cell death, attributable to either necrosis or apoptosis. Also preterm birth is associated with significant neurological disability and chronic hypoxia. In chronic hypoxia, vascular growth factor (VEGF) and brain-derived neurotrophic factor (BDNF) function in neurovasculature by prominent angiogenesis and block of neuronal apoptosis. BDNF, a family of neurotrophic factors has roles of neuroprotection and neural growth, axonal guidance, through the activation of tyrosine receptor kinases B (TrkB) receptor. Also BDNF rescues brain-derived endothelial cells from hypoxic damage,and induces angiogenesis in hypoxic cultured model. Moreover ther are some possibilities of 'cross-talk of signaling' between BDNF and VEGF. These studies suggest that BDNF and VEGF are candidates for the pharmacological intervention in hypoxic neuronal injury.
비치명적 만성 저산소증에서 뇌의 신경 혈관계의 적응: Neurotrophic factor 의 역할
김강련 고신대학교(의대) 고신대학교 의과대학 학술지 2006 고신대학교 의과대학 학술지 Vol.21 No.1
Hypoxia episodes of prenatal and perinatal brain are causes of significant long-term neurologic morbidity, mental retardation, seizures, and cerebral palsy, owing to the neuronal cell death, attributable to either necrosis or apoptosis. Also preterm birth is associated with significant neurological disability and chronic hypoxia. In chronic hypoxia, vascular growth factor (VEGF) and brain-derived neurotrophic factor (BDNF) function in neurovasculature by prominent angiogenesis and block of neuronal apoptosis. BDNF, a family of neurotrophic factors has roles of neuroprotection and neural growth,axonal guidance, through the activation of tyrosine receptor kinases B (TrkB) receptor. Also BDNF rescues brain-derived endothelial cells from hypoxic damage,and induces angiogenesis in hypoxic cultured model. Moreover there are some possibilities of 'cross-talk of signaling1 between BDNF and VEGF. These studies suggest that BDNF and VEGF are candidates for the pharmacological intervention in hypoxic neuronal injury.
A Study on Chromosome of K₅₆₂ Cells for the Use of Target Cells in Human Natural Killer Cell Assay
김강련 고신대학교(의대) 고신대학교 의과대학 학술지 1986 고신대학교 의과대학 학술지 Vol.2 No.1
The metaphase chromosomes of the K₅₆₂ Cell lines stained with trypsingiemsa method were observed and the results obtained are summarized as fellowing; 1) The chromosome types were classified according to the position of centromere. 2) The K₅₆₂ cells were aneuploid with a prominent, pseudotriploid mode. 3) Chromosome numbers were varied from 43 to 75. 4) The most frequent chromosomal number was 6027.0%) 5) Single chromatid break were observed in the long arms of chromosome No.6 and No.11 6) In the karyotypic analysis, it was appeared that all the cells contained at least four markers.
사람의 Natural Killer Cell Assay의 標的細胞로 利用되는 K_(562)細胞의 染色體에 관한 硏究
金岡鍊 고신대학교 의학부 1986 高神大學校 醫學部 論文集 Vol.2 No.1
The metaphase chromosomes of the K_(562) cell lines stained with trypsingiemsa method were observed and the results obtained are summarized as fellowing; 1) The chrdmosome types were classified according to the position of centromere. 2) The K_562 cells were aneuploid with a prominent, pseudotriploid mode. 3) Chromosome numbers were varied from 43 to 75 4) The most frequent chromosomal number was 60(27.0%) 5) Single chromatid break were observed in the long arms of chromosome No.6 and No.11 6) In the karyotypic analysis it was appeared that all the cells contained at least four markers.
鼠의 Natural Killer Cell Assay의 標的細胞로 利用되는 Yac-1細胞의 染色體에 관한 硏究
金岡鍊 고신대학교 의학부 1986 高神大學校 醫學部 論文集 Vol.2 No.1
The metaphase chromosomes of the Yac-1 cell stained with trypsingiemsa method were observed and the results obtained are summarized as fellowing; 1) The basic karyotype of the Yac-1 cells was consisted of 40 chromosomes. 2) The chromosome types classified according to the position of centromere were acrocentric to telocentric in 20 pairs of the whole. 3) The staining intensity of the band recorded in four degrees were, very dark in 23, medium dark 19, weakly positive in 16, pale in 38 bands. And the total number of bands was recorded as 96 in 20 pairs of the whole.