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김갑정,이인수,박경량 한국생명과학회 1998 생명과학회지 Vol.8 No.3
As a part of a study on the development of microbiological methods for petroleum exploration, the distribution of the avarafe survival rate for heterotrophs to various heavy metal ions and hydrocarbons were surveyed and compared by the use of plate count method. In consequence of the avarage survival rate to heavy metal ions(2 hours treatment) and hydrocarbons(1 hour treatment) for heterotrophs isolatinf from soil samples(50cm depth) which located in Doum mountain(A, B and D site) and Aedowon(C site) at Pohang area, the survival rate of heterotrophs for nickel(600ppm), cobalt(500ppm), cadmiun(100ppm), mercury(20ppm), zinc(400 ppm) and lead(500ppm) were 73.7%, 82.6%, 76.8%, 9.5%, 77.8% and 73.6% at A site and 67.9%, 82.5%, 86.0%, 5.8%, 82.5% and 91.7% at B site, 87.8%, 79.8%, 87.5%, 7.0%, 84.2% AND 47.7% AT c SITE, AND 71.8%, 76%, 85.9%, 1,2%, 79.6% AND 88.3% AT D site, respectively. Also the survival rate of heterotrophs from A,B,C and D site to pentane and hexane(each concentration is 20%) were 26.7% and 42.5%, 11.8% and 8.1%, 44.3% and 36.2%, and 12% and 3.5%, respectively. therefore, heterotrophs from B and D site that alternated gravelstone, muddy sandstone and sandstone were higher survival rate to the heavy metal ions than heterotrophs from A site which mainly composed gravelstone. Also, heterotrophs from C site which mainly composed muddy sandstone and once produced natural gas were showed relatively higher survival rate to the heavy metal ions and hydrocarbons than the other sites. Consequently, we confirmed that the distributions of tolerant heterotrophs to heavy metal ions and hydrocarbons were differ from the lithological compositon.
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김갑정,임진아,박성주,문형태,박경량,이인수,Kim, Gab-Jung,Lim, Jin-A,Park, SeongJoo,Mun, Hyeong-Tae,Park, Kyeong-Ryang,Lee, In-Soo 한국생명과학회 1998 생명과학회지 Vol.8 No.3
In order to clarify the effects of acid rain on soil microorganisms, the inpact of acid to soil microorganisms was survyed for 14 weeks using soil microcosms from industrial site A and B, Gaejok mountain, and Daechong lake in Taejeon area. The acid tolerant-microorganisms in natural soil, using culturing method were counted to be 5.8 - $8.0{\times}10^6$CFU/g soil. The number of microorganisms using ATP-biomass analysis for natural soil samples were also analyzed and 2.2 - $2.6{\times}10^9$ cell/g soil in industrial site A and B, Gaejok mountain, and Daechong lake were determined. In soil samples, which were treated with artificial acid rain, the number of acid tolerant microorganisms were counted 2.9 - $5.8{\times}10^5$ and 2.8 - $7.5{\times}10^8$, respectively. Therefore, we conformed that the numver of soil microorganisms were influenced by acid rain. Also, long term acid tolerant microorganisms were identified as Rhodotorula sp. and Pseudomonas sp.
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진범식,최주연,최진영,김갑정,기미경,김준명,김성순 대한의학회 2009 Journal of Korean medical science Vol.24 No.6
Resistance assays are useful in guiding decisions for patients experiencing virologic failure (VF) during highly-active antiretroviral therapy (HAART). We investigated antiretroviral resistance mutations in 41 Korean human immunodeficiency virus type 1 (HIV-1) infected patients with VF and observed immunologic/virologic response 6 months after HAART regimen change. Mean HAART duration prior to resistance assay was 45.3±27.5 months and commonly prescribed HAART regimens were zidovudine/lamivudine/nelfinavir (22.0%) and zidovudine/lamivudine/efavirenz (19.5 %). Forty patients (97.6%) revealed intermediate to high-level resistance to equal or more than 2 antiretroviral drugs among prescribed HAART regimen. M184V/I mutation was observed in 36 patients (87.7%) followed by T215Y/F (41.5%) and M46I/L (34%). Six months after resistance assay and HAART regimen change, median CD4+ T cell count increased from 168 cells/μL (interquartile range [IQR], 62-253) to 276 cells/μL (IQR, 153-381) and log viral load decreased from 4.65 copies/mL (IQR, 4.18-5.00) to 1.91 copies/mL (IQR, 1.10-3.60) (P<0.001 for both values). The number of patients who accomplished viral load <400 copies/mL was 26 (63.4%) at 6 months follow-up. In conclusion, many Korean HIV-1 infected patients with VF are harboring strains with multiple resistance mutations and immunologic/virologic parameters are improved significantly after genotypic resistance assay and HAART regimen change.
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