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Escherichia coli의 시티딘/디옥시시티딘 디아미나제를 코드하는 cdd 유전자의 클로닝
권택규,김태호,황선갑,김종국,송방호,홍순덕 한국산업미생물학회 1990 한국미생물·생명공학회지 Vol.18 No.6
E. coli의 cytidine deaminase(cytidine/2'-deoxycytidine aminohydrolase; EC 3.5,4.5)를 코딩하는 cdd 유전자를 E. coli cdd^-pyr^- 결손 변이주를 cloning host로 하여 southern blotting과 colony hybridization을 통하여 클로닝하였다. cdd 유전자가 단편은, cdd 유전자의 transcription initiation 부위의 23개 nucleotide를 합성한 후 probe로 사용하여 Southern hybridization에 의해 회수된 cdd 유전자를 함유한 단편을 얻었으며, 이를 pBR322에 삽입한 후 형질전환하여 colony hybridization한 결과 cdd^+ cell을 얻었다. 삽입된 DNA 단편의 size는 27kb이었으며 이를 결실 및 subcloning을 연속 수행한 결과 2.1kb의 SalI/DraI tragment(pTK605)에 cdd 유전자가 location되어 있음을 알게 되었다. Mini cell 실험결과 합성된 polypeptide는 약 33kDa이었으며, wild type의 cytidine deaminase의 활성이 pBR322에서 증폭시킴으로서 37배 정도 배가되었으며, pBR322에 비해 pUC vector계에서 다시 활성이 7배 정도 증가됨을 알 수 있었다. We have cloned the cdd gene from E. coli C600 using (cdd^-) as a host. From the sequenced promoter region of E. coli cdd gene which has been determined by Valentin-Hansen P. (1985), we synthesized the 23 mer oligonucleotides corresponding to the transcription initiation region and used as a probe for cloning of the cdd gene by Southern blotting. The isolated fragments in the blotting were introduced to the colony hybridization after transforming it into the E. coli JF611 (cdd^-, pyr^- double mutant), and we identified the hybridized band at 27 kb long. From the original insert of 27 kb fragment in the BamHI site of pBR322, the 5.3 kb fragment containing the cdd gene was isolated by subsequent deletion and subcloning. From the derived plasmid pTK509, further deletion and subcloning were performed and clarified that the cdd gene was located in the 2.1 kb of SalI/DraI segment in the insert of pTK605. The polypeptide encoded by the cloned DNA was appeared to be a molecular mass of 33,000.
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권택규 불교학연구회 2021 불교학연구 Vol.66 No.-
Ouyang Jingwu (歐陽 竟無, 1871–1943) is the establisher of modern Chinese Buddhist Study. He was born in the typical Chinese gentry family in Jiangxi province. His family members gained upper gentry degree through the Imperial Examination, but could not take normal central government posts. His uncle Ouyang Xu educated Ouyang Jingwu who had lost his father in his early age. While traveling around the country as a private assistant, Xu was also keen on social engagements, including disseminating new education in Jiangxi province. This was a combination of the modern intellectuals’ sense of engagement and the traditional gentry class’ concept of social responsibility. After receiving traditional education such as Confucian scriptures and literature in his hometown, Jingwu learned statecraft theory and Western study at Jingxun Shuyuan in Nanchang, Jiangxi province. While the political movement swept the intellectual class at the time, Jingwu turned his perspective into human nature through Lu-Wang school theory, which laid the ideological foundation for him to turn to Buddhism. On the other hand, he established a school in his hometown and introduced new educational methods. This was directly influenced by his uncle Ouyang Xu, indicating his self-awareness as a gentleman who lived during the transition period of the country. At the same time, it was in line with Yang Renshan's intention to reform Chinese Buddhism through the new educational system, and became an important reason for Yang's choice of Ouyang Jingwu as his academic successor. 구양경무(歐陽竟無)는 금릉각경처를 세운 양인산(楊仁山)의 학문적 유산을 이어 받아 지나내학원을 창설하고 근대 중국불교학을 정립한 선구자로 평가된다. 그는 강서성(江西省)의 경제적으로 유족하지 못한 전형적인 재지신사가문에서 태어났다. 구양 경무의 조부, 부친, 숙부들은 모두 과거를 통해 상층신사지위를 얻었고 교육을 통해 뛰어난 고문실력을 갖추었지만 정상적인 중앙관직에 취임하지 못하고 미관말직이나 막료로 전국을 전전하였다. 숙부 구양욱(歐陽昱)은 막료로서 전국을 돌아다니면서도 일찍 상부(喪父)한 구양경무를 직접 양육・교육하고 강서성에서 신교육을 보급하고 잠상(蠶桑)을 장려하는 등 사회적 실천에도 힘썼다. 이는 근대적 지성인의 현실참여의식과 전통 신사계층의 사회적 책무관념이 결합된 것으로서 후일 구양 경무에게도 큰 영향을 미치게 되었다. 구양 경무는 고향에서 경학과 문학 등의 전통교육을 받은 후 강서성 남창(南昌)의 경훈서원(經訓書院)에서 경세학과 서학 등의 신학문을 섭렵하였다. 정치운동의 파고가 당시 지식인층을 휩쓰는 와중에 구양경무는 오히려 육왕심학을 통해 인간 내부로 시각을 돌리기 시작했다. 이는 이후 구양 경무가 불교로 전향할 수 있었던 사상적 토대를 마련했다. 다른 한편 고향에서 학교를 설립하여 새로운 교육방법을 도입하는 등 새로운 교육 이념을 실천하였다. 이는 숙부 구양 욱의 직접적인 영향을 받은 것으로 전환기를 살았던 신사계층으로서의 자각을 보여준다. 동시에 새로운 교육체계를 통해 중국불교를 개혁하려 했던 양인산의 의도에 부합하여, 구양 경무가 양인산의 학문적 적통을 이어받을 수 있었던 중요한 원인이 되었다.
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항암제로서의 TNF-Related Apoptosis-Inducing Ligands (TRAIL)
권택규,이태진 대한암예방학회 2006 Journal of cancer prevention Vol.11 No.1
TRAIL have been widely viewed as potential cancer therapeutics because of its specific apoptosis- inducing capacity in tumor cells. The increased understanding of apoptosis pathways has directed attention to components of death receptor mediated apoptotic pathway as potential targets not only for chemotherapeutic but also for chemopreventive agents. An increasing number of previously identified chemotherapeutic and chemopreventive agents were found to increase the sensitivity to TRAIL-mediated apoptosis in a variety of premalignant and malignant cell types in vitro and in a few animal models in vivo. Also, phase 1 and 2 clinical trials using agonistic mAb that engage the human TRAIL receptor DR4 and DR5 have also provided encouraging results in cancer patients. Thus, understanding the control of death receptor-mediated apoptosis by chemotherapeutic and chemopreventive agents and their implications in cancer treatments may provide a rational approach for using such agents alone or in combination with TRAIL to enhance TRAIL-mediated apoptosis. In this study, we summarize biological agent and drug that sensitize tumors to TRAIL-mediated apoptosis and discuss the potential molecular basis for their sensitization. (Cancer Prev Res 11, 9-21, 2006)
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Caustic Failure of Stainless Steel Dosing Valve in LNG Carrier
권택규(Taek-Kyu Kwon),한종만(Jong-Man Han) 한국마린엔지니어링학회 2006 한국마린엔지니어링학회 학술대회 논문집 Vol.2006 No.-
DSME Analyzed cracked dosing valve of LNG Carrier Boiler and carried out some test to find the causes of such failure.
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김은옥,권택규,최상원 한국식품영양과학회 2014 Journal of medicinal food Vol.17 No.5
The purpose of this study was to investigate how proliferation and apoptosis in human leukemia U937 cells are affected by four hydroxycinnamic acid derivatives (HCADs) in corn (Zea mays L.) bran: p-coumaric (CA), ferulic acids (FA), dicoumaroylputrescine (DCP), and diferuloylputrescine (DFP). Of the four HCADs, DFP dose dependently exerted the strongest cytotoxic effect and induction of apoptosis in the U937 cells. In addition, DFP induced distinct morphological changes characteristic of cellular apoptosis, such as chromatin condensation, apoptotic bodies, and DNA fragmentations. The DFP-induced apoptosis was also associated with released cytochrome c in the cytosol with activation of caspase 3, together with the downregulation of anti-apoptotic proteins, including XIAP and cIAP2, Bcl-2, and Mcl-1. Finally, the DFP-induced apoptosis was a cell-specific response in leukemia cells, as compared with those of other cancer cells, such as Caki, HT29, SKHep1, and MDA-MB231. Thus, these results suggest that DFP may be useful as a potential source of natural antileukemic agents.
ScienceON
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