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중합효소 연쇄반응을 이용한 restriction fragment length polymorphism 법의 Helicobacter pylori 균주 감별에 대한 유용성
박철희(Chul Hee Park),이상우(Sang Woo Lee),박동규(Dong Kyu Park),김경진(Kyoung Jin Kim),권소임(So Im Kwon),진윤태(Yoon Tae Jeen),전훈재(Hoon Jai Chun),이홍식(Hong Sik Lee),송치욱(Chi Wook Song),엄순호(Soon Ho Um),최재현(Jai Hyun Choi 대한내과학회 2001 대한내과학회지 Vol.60 No.4
N/A Background: The aim of this prospective study was to evaluate the usefulness of PCR-based restriction fragment length polymorphism (RFLP) analysis for differentiating H. pylori strains after the triple therapy in patient s with duodenal ulcer. Methods: Following a 1-2 week regimen of omeprazole 40 mg, amoxicillin 2.0 g, and clarithromycin 1.0 g, twice daily, twenty patient s with duodenal ulcer were enrolled. Ten patients (group 1) were not successfully treated, and another 10 patients (group 2) exhibited recurrence of infection. Follow-up diagnosis was performed by Giemsa stain and CLO test. RFLP profiles of antral and midbody biopsy specimens were compared before and after therapy. PCR products using the ure C gene were digested with restriction enzymes Hha I, M bo I, and H ind III, and the fragments generated were analyzed by agarose gel electrophoresis. Results: Hha I, M bo I, and H ind III digestion produced 13, 7, and 2 distinguishable digestion patterns, respectively. There was no difference in RFLP profiles before and after the therapy in 17 duodenal ulcer patient s, while different RFLP profiles following therapy were discovered in 3 patients. Following treatment, one (group 2) patient differed in M bo I, and two (one each from both groups) patients differed in Hha I and M bo I RFLP patterns. Conclusion: This study supports the hypothesis that PCR-based RFLP analysis can be useful for differentiating reinfection and recrudescence of H . pylori strains following triple therapy. (Korean J Med 60:324- 329, 2001)