얼부 한국인의 Rh표현형 벤도

권계철 ( Kye Chul Kwon ),유재관 ( Jae Kwan Yu ),박숙자 ( Sook Ja Pak ),주현정 ( Hyun Jeong Ju ) 대한임상검사과학회 1998 대한임상검사과학회지(KJCLS) Vol.30 No.2

In routine blood bank, Rh phenotypes including C, c, E, e, were hardly requested and performed. But Rh antibodies causes fetal hydrops, hemolytic anemia, Knowing about the frequencies of Rh phenotypes to find appropriate matched blood is important. Beause the frequencies of Rh phenotypes of Korean were incomplete still, the study for observing Rh phenotypes was performed among the 77,627 blood donors in Daejon Chungnam The results were as follows 1. The incidences. of Rh-D antigen negative were 231 cases(U.3%) respectively among 77,627 donors. 2. The incidences of Rh-Hr antigen were C 88.3%, c 55.3%, E 50.4%, e 90.8% among all Rh-D positive 349 case and C 42.1%, c 93.0%, E 16.7%, e 98.2% among all Rh-D negative 228 case. 3. Rh phenotype of 349 Rh positive and 228 Rh negative blood donors are categorized 15 patterns. And the incidence were CDe 43.3%, CcDEe 37.0%, cDE 8.5%, CcDe 6.3%, cDEe 3.4%, CDEe 1.5%, CcDE 0.3% and cde 48.2%, Ccde 30.3%, cdEe 8.8%. cdE 0.4%. CdEe 1.3%. CcdE 0.4. CdE 0.9%. de 0.4%. In conclusion, Rh phenotypes of Koreans were variable and Rh blood phenotype tests would be a routine blood test to prevent from aloimmunization and hemolytic anemia resulted in Rh mismatch blood transfusion.

임상검체에서 분리된 병원성 사상균의 분자생물학적 분석

이장호,권계철,구선회,Lee, Jang Ho,Kwon, Kye Chul,Koo, Sun Hoe 대한임상검사과학회 2020 대한임상검사과학회지(KJCLS) Vol.52 No.3

임상 검체에서 분리된 65개의 사상형 진균을 대상으로 연구하였다. 이 균주들은 형태학적으로 동정이 불가능한 진균, 형태학적으로 유사하여 동정이 까다로운 균주, 종(species) 수준의 동정이 요구되는 균종들이다. PCR과 염기서열분석은 ITS. DiD2, 그리고 β-tubulin 유전자를 표적 부위로 하였고, 증폭된 염기서열은 상동성 분석을 위하여 GenBank 데이터베이스의 알고리즘을 이용하여 분석하였다. 형태학적으로 속 수준의 동정이 가능한 진균은 61.5%이었고, 65주의 염기서열분석으로 62 균주는 속과 종의 동정이 가능하였다. 형태학적 검사와 염기서열분석의 결과, 속과 종이 불일치한 경우 14주(21.5%)이었고, 형태학적으로 동정이 불가능하였던 사례는 11 균주이었다. B. dermatitidis, T. marneffei, 그리고 G. argillacea 등은 염기서열분석으로 국내에서 처음으로 확인하였다. Aspergillus와 같이 흔히 분리되고 성장이 빠른 진균들의 경우에는 형태학적인 검사가 보고시간과 비용 면에서는 매우 유용한 방법이다. 분자유전학적인 검사 방법은 비용과 임상적 중요성 등을 고려하여야 하지만 분자유전학적 검사를 병행하여 신속하고 정확한 결과를 제공할 수 있다. Sixty-five molds isolated from clinical specimens were included in this study. All the isolates were molds that could be identified morphologically, strains that are difficult to identify because of morphological similarities, and strains that require species-level identification. PCR and direct sequencing were performed to target the internal transcribed spacer (ITS) region, the D1/D2 region, and the β-tubulin gene. Comparative sequence analysis using the GenBank database was performed using the basic local alignment search tool (BLAST) algorithm. The fungi identified morphologically to the genus level were 67%. Sequencing analysis was performed on 62 genera and species level of the 65 strains. Discrepancies were 14 (21.5%) of the 65 strains between the results of phenotypic and molecular identification. B. dermatitidis, T. marneffei, and G. argillacea were identified for the first time in Korea using the DNA sequencing method. Morphological identification is a very useful method in terms of the reporting time and costs in cases of frequently isolated and rapid growth, such as Aspergillus. When molecular methods are employed, the cost and clinical significance should be considered. On the other hand, the molecular identification of molds can provide fast and accurate results.

다발성골수종 환자의 파라핀포매조직에서 MicroRNA 발현

최우순 ( Woo Soon Choi ),권계철 ( Kye Chul Kwon ) 대한임상검사과학회 2015 대한임상검사과학회지(KJCLS) Vol.47 No.4

Research of thyroid cancer, liver cancer, and lung cancer has been reported in Korea. However microRNA research of multiple myeloma has never been reported. Hence we intended to confirm whether microRNA can be utilized as a diagnostic marker to patients of multiple myeloma. We also intended to evaluate whether microRNA can be detected in paraffin-embedded tissue (FFPE). This research was conducted targeting 8 samples from patients of multiple myeloma who do not have any other diseases, and 2 control samples. From January 2010 to July 2012, we selected miR-15a, miR-16, miR-21, miR-181a and miR-221 as microRNA target genes. It was decided that for a sample to be significant, the results should show values more than 1.5 or less than -1.5. Our findings of fold change were highly significant in miR-15a with a value of 37.5% (3/8). From these studies, we learned that miR-15a is useful with westerners. miR-221, on the other hand, shows conflicts with westerners, so more research will be needed in this area. In addition, it was confirmed that microRNA can be detected in paraffin embedded tissue (FFPE).

증례 : 혈액종양 ; 만성골수성백혈병 환자의 자발 비장파열에 대한 도관경유색전술 1예

문지영 ( Ji Young Moon ),김지명 ( Ji Myung Kim ),권계철 ( Kye Chul Kwon ),신병석 ( Byung Seok Shin ),엄준영 ( Joon Young Ohm ),조덕연 ( Deog Yeon Jo ),송익찬 ( Ik Chan Song ) 대한내과학회 2015 대한내과학회지 Vol.89 No.5

저자들은 만성골수성백혈병 환자의 초기 증상으로 나타난 자발 비장파열을 도관경유색전술으로 치료하여 성공한 증례를 보고한다. 36세 남자가 내원 1일 전부터 발생한 복통으로 응급실에 방문하였다. 복부의 전산화 단층 촬영에서 매우 거대한 비장비대와 함께 복강내출혈이 관찰되었고, 비장의 하단에 국소적인 파열 및 조영제가 혈관 밖으로 유출되는 것이 관찰되었다. 환자가 혈류역학적으로 안정하였기 때문에 저자들은 수혈을 시행하며 도관경유색전술을 시도하였다. 이후 시행한 골수 흡인 검사에서 만성골수성백혈병의 만성기로 진단되었고, 환자는 시술을 시행한 후 13일째 별다른 부작용 및 합병증 없이 퇴원할 수 있었다. 이로써 저자들은 혈액암 환자에서 자발 비장파열이 발생하였을 때 도관경유색전술을 우선 시행해야 한다고 주장한다. We report a rare case of a patient who presented with pathological splenic rupture as the initial manifestation of chronic myeloid leukemia (CML) and was treated successfully by transcatheter arterial embolization. A 36-year-old man presented to the emergency department with a 1-day history of abdominal pain. Computed tomography showed gross hemoperitoneum with marked splenomegaly, with suspected focal rupture at the lower portion of the spleen and the extravasation of contrast material indicating active bleeding. Given the patient’s hemodynamic stability, he was treated with partial splenic embolization by an interventional radiologist, and transfused with red blood cells. Examination of a bone marrow aspiration and biopsy led to a diagnosis of chronic phase CML. He was discharged from the hospital on day 13 post-embolization. Transcatheter arterial embolization should be considered as the initial treatment of spontaneous splenic rupture, especially in patients with hematological malignancies. (Korean J Med 2015,89:585-588)

건식 화학 분석기 Ektachem 750의 평가

권계철,송인숙 충남대학교 의과대학 지역사회의학연구소 1996 충남의대잡지 Vol.23 No.2

We evaluated a multichannel dry chemical analyzer "Ektachem 750 XRC" for emergency tests according to NCCLS guidelines. Results were fo lowed as below. 1. The evaluation of between-run precision and within-run precision about Na^+, K^+, C1^-, glucose, Cr, ALT, ammonia and amylase revealed high coefficients of variation. 2. In the evaluation of linearity, K^+, glucose, ALT and Cr showed high linearity as r=0.99, r=0.99, r=0.99 and r=0.94 respectively. 3. The correlation studies about Na^+, K^+, Cl^- were performer with NOVA 13. Correlation coefficients of Na^+, K^+ and Cl^- were r=0.93, r=0.99 and r=0.96 respectively. The correlation coefficients of ALT, glucose and Cr with HITACHI 736-20 were r= 0.99, r=0.99 and r=0.94 respectively. We concluded that Kodak Ektachem 750 XRC was suitable as emergency chemistry autoanalyzer because of high precision, linearity and relatively high correlation coefficients.

비교 유전자 보합법을 이용한 한국인 갑상선 유두상 암종에서의 유전자 변화에 관한 연구

김진만,신소영,권계철,설지영,김병국,구선회 충남대학교 의학연구소 2001 충남의대잡지 Vol.28 No.2

Transformation and progression of cancers are thought to be caused by an accumulation of complex genetic alterations, but little is known about specific genetic changes. In this study, the authors have undertaken a genome-wide screening to detect changes in 12 cases of papillary thyroid carcinoma which is the most common subtype of thyroid cancer among Koreans. Comparative genomic hybridization (CGH) was used to screen for DNA sequence gains and losses across all human chromosomes. Biotin-labeled tumor DNA and digoxigenin-labeled normal DNA were hybridized to normal metaphase cells. The fluorescence signals were captured by fluorescence microscope after detection by avidin FITC and antidigoxigenin rhodamine. Then, the ratio of fluorescence was calculated by an image analyzer. The CGH results showed gains on chromosomes 1q(25%), 13q(25%), 17q(25%), 7p(17%), 18q(17%), 17p(8%) and 7q(8%). The Deletions were on chromosomes 22q(25%), 19p(8%), 19q(8%), 20p(8%), and 20q(8%). Usual amplification sites of genome were 1q, 13q, and 17q. The most common deletion site was 22q. The results indicate that the amplification on chromosome 1q, 13q, and 17q and the deletion on chromosomes 22q are the most frequent genetic alterations in papillary thyroid carcinoma among Koreans. The CGH was thought to be very useful in the screening of genetic alterations of solid tumors.

중합효소연쇄 반응을 이용한 Helicobacter pylori 검출

신연식,임춘화,정진홍,최병권,권계철,구선회,박종우 대한임상병리학회 1997 대한임상병리학회지 Vol.17 No.5

한국인 임산부와 신생아에서의Rh아형 및 Kell혈액형 불일치 빈도

이난영,서장수,양동욱,손한철,권계철,유봉재 大韓輸血學會 1998 大韓輸血學會誌 Vol.9 No.1

비교유전자교잡법과 형광동소교잡법을 이용하여 기원을 밝힌 Marker 염색체 1예

신소영,전영미,임춘화,김문희,구선회,박종우,권계철,박윤미 대한임상병리학회 2001 대한임상병리학회지 Vol.21 No.4