내측연수경색으로 인한 반대측 중추성 안면마비

고석민 대한뇌졸중학회 2006 Journal of stroke Vol.8 No.1

The 'classic' triad of medial medullary infarction (MMI) consists of contralateral hemiplegia, loss of posterior column sensation on the contralateral side and involvement of the tongue on the ipsilateral side. Facial muscle is usually spared in MMI. A 51-year-old patient with MMI came to our department. His major clinical manifestations were contralateral hemiparesis, and contralateral central type facial palsy. He had neither sensory change nor tongue deviation. This report support the hypothesis that part of the corticobulbar fibers supplying the lower facial muscles descend ipsilaterally in the ventromedial part of the medulla and then, after decussation, ascend rostrally to the facial nucleus in the dorsolateral part of the medulla.

아이스케키

고석민 대한사립학교장회(구 대한사립중고등학교장회) 2000 私學 Vol.92 No.-

기능획득 돌연변이 인삼 모상근의 대량생산

고석민,인동수,정화지,최동욱,유장렬,Ko, Suk-Min,In, Dong-Soo,Chung, Hwa-Jee,Choi, Dong-Woog,Liu, Jang-Ryol 한국식물생명공학회 2007 식물생명공학회지 Vol.34 No.4

본 연구는 아그로박테리움 공동배양법을 이용한 기능획득 인삼 모상근의 대량생산을 위한 조건 확립에 대한 것이다. 일반적으로, 인삼과 같이 형질전환을 통한종자의 확보가 어려운 식물에서는 loss-of-function을 이용한 기능유전체 연구에 한계가 있다. 한편, 유전자의 기능을 활성화시키는 방법 (gain-of-function)인 activation tagging 기술은 이러한 문제점을 극복할 수 있는 대안이 될 수 있으며, Agrobacterium rhizogenes를 이용한 모상근 생산 시스템은 대량의 돌연변이체를 안정적으로 용이하게 얻을 수 있다는 점에서 최적의 시스템이라고 할 수 있다. 본 연구에서는 activation-tagging된 효율적인 형질전환 모상근 생산에 있어서의 최적의 아그로박테리움 균주 및 인삼조직, 배지조성 등에 대한 조건을 확립하였으며, 다양한 배지에서의 형질전환 모상근의 생장률 및 분지율, 표현형 등을 조사하였다. 엽병 절편을 activation-tagging vector pKH01을 가지고 있는 A. rhizogenes R1000와 공동배양하였을 때 배양 4주후 85.9%의 빈도로 모상근이 생산되었다. 모상근의 최대 생장과 분지도를 나타내는 배양조건을 조사한 바 엽병절편을 1/2 SH 배지에서 4주 배양하였을 때 왕성하게 생장하였으며 2.6의 분지도를 보여주었다. 최종적으로 1,989개체의 독립적인 형질전환 모상근 line을 생산하였으며, 이들 모상근 line은 인삼 진세노사이드 생합성 관련 유전자의 발굴 및 기능해석에 유용하게 쓰일 것이다. This study describes conditions for the mass production of activation-tagged mutant hairy root lines of ginseng by cocultivation with Agrobacterium rhizogenes. Because it is not currently possible to produce progeny from transgenic ginseng, a loss-of-function approach for functional genomics cannot be appliable to this species. A gain-of-function approach is alternatively the choice and hairy root production by cocultivation of A. rhizogenes would be most practical to obtain a large number of mutants. Various sources of explants were subjected to genetic transformation with various strains of A. rhizogenes harboring the activation-tagging vector pKH01 to determine optimum conditions for the highest frequency of hairy root formation on explants. Petiole explants cocultivated with A. rhizogenes R1000 produced hairy roots at a frequency of 85.9% after 4 weeks of culture. Conditions for maximum growth or branching rate of hairy roots were also investigated by using various culture media. Petiole explants cultured on half strength Schenk and Hildebrandt medium produced vigorously growing branched roots at a rate of 2.6 after 4 weeks of culture. A total of 1,989 lines of hairy root mutants were established in this study. These hairy root lines will be useful to determine functions of genes for biosynthesis of ginsenosides.

일일초에서의 기능획득 돌연변이 모상근의 대량생산

고석민,정화지,이호연 한국자원식물학회 2011 한국자원식물학회지 Vol.24 No.5

This study describes conditions for the mass production of mutant hairy root lines by co-cultivation with A. rhizogenes harboring the activation tagging vector pHC7. Various sources of explants were subjected to genetic transformation with A. rhizogenes to determine optimum conditions and cultivar for the highest frequency of hairy root formation on explants. Hairy root formation also were investigated in transformed hairy roots grown in various culture media. Finally, a total of approximately 2,500 lines of hairy root mutants were produced in this study. A managing system for metabolomics in hairy root lines also were established. These hairy root lines will be useful to determine functions of genes relating biosynthesis pathway of secondary metabolites. 본 연구는 아그로박테리움을 이용한 기능획득 일일초 모상근의 대량생산을 위한 조건 확립에 대한 것이다. 본 연구에서는, 효율적인 형질전환 일일초 모상근 생산에 있어서의 최적의 일일초 품종의 선발과 최적의 일일초 조직을 결정하였으며, 또한 다양한 배지에 있어서의 모상근 유도를 조사하였다. 최종적으로 약 2,500개의 독립적인 형질전환 일일초 모상근 line을 생산하였으며, 또한 이들을 이용하여, 대사체 연구를 위한 효율적 관리 시스템을 구축하였다. 이들 모상근 line은 일일초 인돌알칼로이드 생합성 관련 유전자의 발굴 및 기능해석에 유용하게 쓰일 것이다.

Cellular Mesoblastic Nephroma with Liver Metastasis in a Neonate: Prenatal and Postnatal Diffusion-Weighted MR Imaging

고석민,김명준,임영재,박국인,이미정 대한영상의학회 2013 Korean Journal of Radiology Vol.14 No.2

Congenital mesoblastic nephroma (CMN) is the most common renal tumor in the first year of life. Here, we present unique findings of cellular variant CMN seen on prenatal and postnatal MRI with diffusion-weighted imaging (DWI).The mass was well-visualized on prenatal MR DWI with diffusion restriction in the solid portions. After excision of the mass, follow-up whole body MRI with DWI helped identify local tumor recurrence with suspicious liver metastasis. This hepatic lesion also showed diffusion restriction.

Expression of the Protective Antigen for PEDV in Transgenic Duckweed, Lemna minor

고석민,선현진,오명진,송인자,김민재,신현숙,고창효,김용우,임평옥,이효연,김석원 한국원예학회 2011 Horticulture, Environment, and Biotechnology Vol.52 No.5

Duckweeds are small, floating aquatic plants with a number of useful characteristics, including edibility, fast-growing, and a clonal proliferation. Duckweed is also fed to animals as a diet complement because of its high nutritional value. Porcine epidemic diarrhea virus (PEDV) is a major causative agent of fatal diarrhea in piglets and is a serious problem in the hog-raising industry. In this study, we assessed the feasibility of producing a protective antigen for the PEDV spike protein 1using duckweed, Lemna minor. Stably transformed Lemna were obtained by co-cultivation with A. tumefaciens EHA105 harboring the PEDV spike protein gene. Transgene integration and expression of the PEDV spike protein 1 gene were confirmed by genomic PCR and RT-PCR and western blot analysis of transgenic Lemna, respectively. This is the first report of the expression of a vaccine antigen against an animal infectious disease in duckweed.

Implant 보철물 access hole의 깊이에 관한 연구

고석민,변태희,이재봉,Ko Sok-Min,Byun Tae-Hee,Lee Jai-Bong 대한치과보철학회 2002 대한치과보철학회지 Vol.40 No.4

A total of 605 implant fixture prosthesis delivered by 3 clinics and 2 laboratories were examined in this study, The object of this study was to determine the proper length of screw head. The depth of access hole were measured and compared to the type of fixture, abutment, gold screw and prosthesis. The results were as follows : 1 The average number of fixtures per patient were 2.97. 2. The number of fixture installed in the upper posterior area are 327(55.56 %), the upper posterior area 171 (28.25%). 3. The depth of access hole is 4.23 mm in shallow area, and 5.46 mm in deep area and the differences were 1.23 mm. 4. The average depth of the aceess hole of the UCLA abutment were 5.02 mm. 5. The number of 4-5 mm access hole depth were 60(22.39%) in abutment screw level and the number of 4-5 mm depth in fixture level were 101 (29.19%). 6. In the shape of screw head, hexed type were 576(95.21%), slotted type were 29(4.79%).

고령자 삼킴기능저하의 평가와 중재

고석민,이지나 대한노년치의학회 2022 대한노년치의학회지 Vol.18 No.2

Korea is on the verge of becoming a super-aged society, and the structure of the disease is changing accordingly. One of the measures to lower the medical and social burden for the elderly is early detection and prevention of elderly frailty. Oral frailty in elderly is reported to be manifested prior to whole body frailty. So, if oral frailty is diagnosed and treated early, independency in mobility and healthy aging of elderly can be effectively promoted. The Korean Academy of Geriatric Dentistry launched establishing a clinical guideline of oral frailty for Korean elderly with diagnostic criteria and treatment modality. If two out of the six criteria, chewing function, occlusal force, tongue force, salivary gland function, swallowing function, and oral hygiene, are checked as not ‘normal’ but ‘at risk’, the patient is diagnosed as having oral frailty. In this paper, evaluation methods and exercise therapies for swallowing function (out of the six criteria for oral frailty) is described in detail.

골밀도 평가방법과 임플란트 안정성 변화의 관찰

고석민,박성재,김인수,송승일,이인경,Ko, Sok-Min,Park, Sung-Jae,Kim, In-Soo,Song, Seung-Il,Lee, In-Kyung 대한치주과학회 2009 Journal of Periodontal & Implant Science Vol.39 No.2

Purpose: The aim of this study was to examine the correlation of the subjective and the objective evaluation of edentulous ridge bone quality, and to evaluate the change of the dental implant stability in each bone density group for early healing period after implant installation. Methods: Sixty-seven implants(Osstem implant$^{(R)}$, Seoul, Korea) were included in this study. We evaluated the bone density by 2 methods. The one was the subjective method which was determined by practitioner s tactile sense, the other was the objective bone type was based on Hounsfield units. The implant stability in each bone type group was assessed by resonance frequency analyzer(Osstell mentor$^{(R)}$). Data were analyzed for the change of the implant stability, and they were compared to verify the difference of groups at the time of installation, 2, 6, 10, 14 weeks postoperatively. Spearman's correlation was used to demonstrate the correlation between the subjective and the objective evaluation of the bone density, and analysis of variance(ANOVA) was used to analyze the differences of implant stability at each time point. Results: There was no close relation between the subjective and the objective evaluation of the bone density(r=0.57). In the subjective groups, there was statistically significant difference between the type 1 and 3 at 10 weeks and between the type 2 and 3 at 14 weeks. In the objective groups, there was no statistically significant difference between the D 1, 2, 3, 4, and 5 group with regard to RFA from baseline to 14 weeks(P>0.1). Conclusions: The implant stability increased over time during the study, and it was improved with bone density proportionally after 2weeks postoperatively. It is recommended that the decision of bone density is base on Hounsfield unit for implant loading time.

백혈구감소증 후에 일과성으로 관해된 중증근무력증

고석민,배종석,안진영,김민기,김병준 대한임상신경생리학회 2006 Annals of Clinical Neurophysiology Vol.8 No.2

Various immunotherapeutic modalities have been used based on the autoimmune pathogenic mechanisms of myasthenia gravis (MG). Cell-mediated immunity as well as auto-antibodies may play a role in the remission and relapse of MG. We recently experienced two patients with MG who showed spontaneous remission fter inadvertent severe leukopenia. These findings suggest that the cell-mediated immune process is important in the treatment of MG, and selective suppression of leukocyte may induce remission in the patients with intractable MG.