Pre - Strain 을 준 316 스테인레스강의 크리프 특성

고상돈,유연철 대한금속재료학회 1985 대한금속재료학회 학술대회 개요집 Vol.1985 No.0

고사리 (Pteridium aquilinum) Ethanol 추출액(抽出液)에 의한 혈압강하작용(血壓降下作用)

고상돈,김기순,Koh, Sang-Don,Kim, Kee-Soon 대한생리학회 1984 대한생리학회지 Vol.18 No.2

The death of cattle from acute bracken poisoning has been recognized for many years. Acute bracken poisoning is characterized by mucoidal nasal and anal hemorrhage, severe anorexia. pyrexia, gastric ulcer and myocardial damage. In 1958 Evans first suggested that clinical picture of bracken poisoning was very much similar to that of radiation injuries such as aplastic anemia, leucopenia, thrombocytopenia and increased capillary fragility. Bracken has been clearly demonstrated to contain a carcinogen as well as thiaminase. However, the nature of carcinogen in bracken has not definetely elucidated. Also it was warned by several workers that bracken could be a causative factor for stomach cancer in Korean and Japanese. It appears that little is known on the e(feet of bracken on the function of cardiovascular system. Therefore the present study was designed to explore effects of ethanol extract of raw and toiled bracken (RBEE:BBEE) on blood pressure in cats. Also studied was the mechanism underlying changed in blood pressure of cats by bracken. The result obtained were as follows; 1) Mean arterial blood pressure was invariably decreased following administration of either RBEE or BBEE. Tn general depressor responses to RBEE persisted longer than that to BBEE. Generally, depressor responses were proportional to debases of RBEE and BBEE administered. 2) After administration of 60 mg/kg RBEB and BBEE, blood Pressure decreased by $62.1{\pm}1.7mmHg$ and $68.0{\pm}3.0mmHg$, respectively. No change was observed between depressor responses to RBEE and that to BBEE. 3) Depressor responses to BBEE and RBEE were not affected by vagotomy, propranolol and regitine. 4) In atropinized animal depressor responses to BBEE and RBEE were reduced by 30-40% showing part of depressor response was resulted from cholinergic effect of bracken.

Acetaldehyde 및 Nicotine에 의한 가토심장 동방결절세포의 활동전압의 변동

김기순,김진혁,강석한,고상돈 한양대학교 의과대학 1987 한양의대 학술지 Vol.7 No.2

Acetaldehyde, a metabolite of ethanol has not only been implicated as a contributory factor that cause alcoholic cardiomyopathies but also has been shown to have significant cardiovascular effects. The suggestion has also been made that acetaldehyde has more pronounced effect on the cardiovascular system than ethanol. Several epidemiological studies have demonstrated that alcoholism is associated with heavy smoking thereby increasing the risk of developing various ailments. Also nicotine has been known to influence the cardiovascular functions profoundly. In the present study, changes in the intracellular action potential were investigated in the sinoatrial node cells of the rabbit heart for 45 minutes following superfusion with Tyrode solutions containing acetaldehyde and/or nicotine. Superfusion of the sinus node cells with acetaldehyde (0.01%-0.1%) resulted in a concentration dependent reduction of action potential amplitude(APA). At the high dosage level (0.1%) changes in APA peaked at 10 minutes and APA was restored to the control level in 45 minutes after onset of superfusion. And Vmax(maximum rate of depolarization) of the sinus node cell was also decreased by acetaldehyde. The rate of spontaneous firing (RSF) of the sinus node cells was markedly decreased by acetaldehyde (0.05%-0.1%), but the action potential duration manifested as ??(time to 60% repolarizaton) was not alatered. On the other hand, nicotine (0.05%) increased APA, prolonged ?? and significantly decreased RSF of the sinus node cells. The maximal changes in APA and ?? were produced at 10 minutes while that in RSF at 7 minutes after onset of superfusion. Following simultaneous superfusion with acetaldehyde and nicotine, APA and RSF of the sinus node cells decreased prominently whereas a transient, small but significant prolongation of ?? was followed by a transient shortening. When the sinus node cells were superfused with acetaldehydee and nicotine simultaneously, it apperared that effects of two drugs on APA were exerted in additive fashion while that of nicotine of RSF was partially antagonized by acetaldeyde.

Tannic Acid가 가토 동방결절 세포 및 심방근 세포의 전기적 특성에 미치는 효과

高相敦,金基淳 한양대학교 의과대학 1988 한양의대 학술지 Vol.8 No.1

Though several workers have demonstrated that intravenous administration of tannic acid elicits a hypotensive response, little study has been made on the direct actin of tannin on the heart. Therefore the present study was undertaken to investigate direct effects of tannic acid on the sinoatrial node and atrial muscle cells of the rabbit heart by means of conventional microelectrode techniques. A superfusion of sinus node cells with tannic acid (0.1, 0.25, 0.5%) resulted in a concencration-dependent reduction of action potential amplitude (APA) and rate of spontaneous firing (RSF), and V max (maximum upstroke velocity)was also decreased by 0.25% tannic acid but action potential duration (APD) was not altered significantly. Following superfusion of atrial muscle cell respectively with 0.1% tannic acid (pH 5.8) and acid-Tyrode solution (pH5.8),ApA and RSF were generally decreased. The effects on APA and RSF of tannin was far more pronounced than that of acid-Tyrode solution. A significant prolongation of APD was observed only after superfusion of atrial muscle fiber with acid-Tyrode solution. The results of present study suggest that tannic acid can exert direct effects on the sinus node and atrial muscle cells generally decreasing APA and RSF.

Tricyclic antidepressants가 고양이 척수후각세포의 통각정보전달에 미치는 효과

고상돈,정수영,신홍기,김기순 한양대학교 의과대학 1992 한양의대 학술지 Vol.12 No.1

To investigate effects of tricyclic antidepressants(TCAs) on the processing of the afferent inputs at the spinal cord level, the responses of wide dynamic range(WDR) cells to mechanical stimulation of the receptive field and to electrical stimulation of the isolated afferent nerve were recorded in the normal cats as well as in the spinalized animals. As an effort to elucidate the mechanism of action of TCA, effects of amitriptyline(AMIT), desipramine(DESI) and clomipramine(CLOM) were observed in the animals treated with naloxone(1mg/kg), cyprogeptadine(2mg/kg) nad yohimbine(100㎍/kg). All the TCAs tested invariably ingibited the responses of WDR cells to mechanical or electrical stimulations. After intravenous administration of CLOM(7mg/kg) and DESI(6mg/kg). the responses to pinch and C-fiber stimulation, and ingibitory effects of these drugs lasted for more than 90 min. AMIT(6mg/kg) elicited only a small but significant ingibition of WDR cell responses to pinch and C-fiber stimulation, and these depressed responses were gradually restored almost to the control level in 60 min after durg administration. TCAs directly applied to the spinal cord did not show any significant influence on the activities of the WDR cells. On the other hand, inhibitory effects of TCAs on all the responses of WDR cells were almost completely abolished in the spinal animals. Following intravenous administration of naloxone or cyproheptadine, ingibitory effect of CLOM was partially blocked. Effect of DESI was completely antagonized by naloxone and partially by yohimbine. These findings suggest that TCA-induced ingibition of WDR cell responses is mediated through the endogenous opioid and monoaminergic descending ingibitory control system.

수종 항경련제가 고양이 척수 후각 세포의 activity에 미치는 효과

고상돈,신홍기,김기순,김태영 한양대학교 의과대학 1993 한양의대 학술지 Vol.13 No.1

The present study was undertaken to investigate effects of a few anticonvulsants on the dorsal horn cell activities, to elucidate their mechanism of action and to examine whether these anticonvulsants have spinal and/or supraspinal actions. Spontaneous activities and the responses of wide dynamic range (WDR) cells to mechanical stimulations and graded electrical stimulations of the afferent nerve were extracellulary recorded in the normal and spinal cats before and after the intravenous administration of diphenylhydantoin (15-30mg/kg, DPH), carbamazepine (15-30mg/kg, CBZ) and valproic acid (15-30mg/kg, VA). Also studied were the effects of picrotoxin and strychnine on the anticonvulsant-induced changes in the dorsal horn cell activities. All the anticonvulsants tested inhibited dose-dependently the response of WDR cell to afferent signals. The resposes of WDR cell to pinch and C-fiber stimulation were more strongly inhibited than those to brush and A-fiber stimulation. Strychnine (0.5mg/kg), not picrotoxin (0.5mg/kg) strongly blocked an inhibitory effect of DPH on all the reponses of WDR cells except C-fiber response. In the spinal animal, approximately two-third of DPH-induced inhibition was maintained. The anticonvulsants also markedly inhibited spontaneous activities of the cat spinal neurons. These results lead to the conclusion that in the lumbar segment of the cats spinal cord most of anticonvulsant-induced inhibitions of the responses of WDR cell to afferent signals were mediated through glycine receptors at both the spinal and supraspinal sites.

Trihydroxy bile acid 가 가토心臟乳頭筋細胞의 膜電位에 미치는 효과

金孝錫,高相敦,金基淳 한양대학교 의과대학 1986 한양의대 학술지 Vol.6 No.1

The experimental observation that intravenous injection of bile acids causes marked circulatory depression with essential failure explains in part, if not entirely, the fact that jaundiced patients have a great tendency to develop bradycardia and hypotension. It has been demonstrated that bile acid, like digitalis and sex steroids, can alter cellular potentials of cardiac cells in animals. Recently it was repoted that glycocholate and taurocholate shortened action potential duration in rabbit ventricular fibers while they did not show any significant effect in rabbit atrial and toad ventricular preparations. The purpose of this study was to investigate effects of cholate, glycocholate and taurocholate on the electrophysiological parameters of the papillary muscle of the rabbit heart. The following results were obtained; 1. Cholate in 0.025% and 0.05% concentration significantly increased magnitude of action potential, overshoot and prolonged actio potential duration of rabbit papillary muscle. 2. Glycocholate in 0.05% concentration decreased action potential of the papillary muscle from 128.0±2.6 mV to 118.5±2.6 mV. In all concentrations glycocholate markedly prolonged action potential duration as manifested by APD(???). 3. The magnitude of cellular potential of the papillary muscle was not altered by taurocholate whereas APD(???) and APD(???) were significantly prolonged by 0.025% and 0.05% taurocholate. 4. Action of bile acids on action potential duration of the papillary muscle was glycocholate>cholate=taurocholate in increasing order.

Nicotine에 의한 자발성고혈압백서(自發性高血壓白鼠)의 혈장 Cholesterol 농도의 변동

류호충,고상돈,신홍기,김기순,Yoo, Ho-Choong,Koh, Sang-Don,Shin, Hong-Kee,Kim, Kee-Soon 대한생리학회 1982 대한생리학회지 Vol.16 No.2

The present study was undertaken to find out fasting total plasma cholesterol levels of SHR at different phases(labile, established and malignant) in the course of hypertension development and also to investigate effect of nicotine on plasma cholesterol levels of these animals. In nicotine administered group, 2.18 mg/kg nicotine was given daily for six weeks. The results obtained are as follows; 1) Mean total plasma cholesterol levels of SHR at labile, established and malignant phases were $104.6{\pm}3.1\;mg/dl$, $120.8{\pm}2.6\;mg/dl$ and $136.6{\pm}2.5\;mg/dl$ respectively. The total plasma cholesterol level generally increased with age of experimental animals. 2) After administration of nicotine (2.18 mg/kg/day) for six weeks, mean total plasma cholesterol levels of SHR at labile, established and malignant phases were $130.2{\pm}3.1\;mg/dl$, $150.4{\pm}3.3\;mg/dl$ and $166.6{\pm}3.2\;mg/dl$, respectively. The result of present study strongly suggests that nicotine has a positive relationship with plasma cholesterol levels in SHR.

Allyl sulfide 및 Garlic oil이 고양이 척수후각세포의 activity에 미치는 효과

홍순기,고상돈,신홍기,김기순 한양대학교 의과대학 1992 한양의대 학술지 Vol.12 No.2

To investigate the effects of garlic on the electrophysiological characteristics of the dorsal horn cell, responses of the wide dynamic range (WDR) cells to mechanical or electrical stimulation were recorded in the cat lumber enlargement following subcutaneous application of garlic-oil,-juice and allyl sulfide respectively of WDR cells to bruch, pressure and pinch were enhanced respectively by 55.0±8.4%, 93.9±21.9% and 51.6±22.1%. In general similar changes in responses of these cells were observed with application of garlic oil into RF. Allyl sulfide tended to produce slight modification of WDR cells activeities, but significant change being recognized only in the response to pressure. The dorsal horn cells which had RF around the digital joint area responded more remarkably to dorsiflexion that to tapping. In the RF expanded by subcutaneous application of either garlic-juice or allyl sulfide, a sensitization appeared to develop especially to pinch stimulation. After application of garlic-juice, the responses of dorsal horn cells to mechanical stimulation increased strikingly, but responses to electrical stimulation were not altered. The results implicate development of sensitization mostly at the peripheral level following subcutaneous application of garlic components in the RF.

Bovine bile에 의한 非痲醉動物의 心脈管系 反應

강돈규,고상돈,김기순 한양대학교 의과대학 1985 한양의대 학술지 Vol.5 No.2

The present study was designed to investigate cardiovascular responses to intravenously administered bovine bile in unanesthetized cats. Also studied were effects of regitine, propranolol and atropine pretreatment on the cardiovascular responses following intravenous administration of bovine bile in conscious animals. The results obtained from the present study were as follows; 1. One minute after 30mg/kg bile administration the heart rate of anesthetized cats increased from 139.6±4.3/min to 157.4±7.6/min probably due to baroreceptor reflex. 2. In conscious cats heart rate decreased from 178.8±7.4/min to 146.0±10.1/min one minute after intravenous administration of bile and the decreased heart rate response persisted for 5 minutes thereafter. 3. Heart rate responses to bile were almost blocked following atropinization of conscious animals. 4. After intravenous administration of 30mg/kg bovine bile depressor responses were observed invariably in both anesthetized(79.2±3.8 mmHg) and unanesthetized cats (60.9±7.6 mmHg). In general depressor response to bile was more pronounced in anesthetized animals while the recovery from decreased blood pressure was more rapid in unanesthetized cats. 5. In conscious animals, depressor responses to bovine bile were partially blocked following regitine pretreatment, while no changes in depressor responses were noticed in either atropine or propranolol treated animals.