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차미선,손홍주,이건,고명선,이상준,이종근 부산대학교 환경문제연구소 1996 環境硏究報 Vol.14 No.1
Microorganisms producing bioemulsifier were isolated from the sea water in Pusan costal aera. The isolated strain which had the highest emulsification activity and stability was identified as the genus Acinetobacter from the results of morphological, cultural and biochemical tests and named Acinetobacter sp. EL-C6 for convinience. The compositions of optimum medium for emulsification of crude oil by Acinetobacter sp. EL-C6 were crude oil 2%, NH_4NO_3 0.2%, K_2HPO_4 0.01%, MgSO_4·7H_2O 1%, CaCl_2·2H_2O 0.1%, NaCl 3% and the initial pH 7.5. The cultivation for emulsification of crude oil was carried out in 500m1 shaking flask containing 100m1 of the optimum medium at 30℃. The highest emulsification was observed after 5 days. The utilization on the various hydrocarbon of the Acinetobacter sp. EL-C6 was showed that the utilization of n-alkane compounds were better than that of aromatic compounds. Among the petroleum compounds, by Acinetobacter sp. EL-06.