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강예재,김기홍 한국수산과학회 2010 Fisheries and Aquatic Sciences Vol.13 No.3
Growth hormone (GH) is known as one of the main osmoregulators in euryhaline teleosts during seawater (SW) adaptation. Many of the physiological actions of GH are mediated through insulin-like growth factor-I (IGF-I), and the GH/IGF-I axis is associated with osmoregulation of fish during SW acclimation. However, little information is available on the response of fish IGF-II to hyperosmotic stress. Here we present the first cloned IGF-I and IGF-II cDNAs of marine medaka, Oryzias dancena, and an analysis of the molecular characteristics of the genes. The marine medaka IGF-I cDNA is 1,340 bp long with a 257-bp 5’ untranslated region (UTR), a 528 bp 3’ UTR, and a 555-bp open reading frame (ORF) encoding a propeptide of 184 amino acid (aa) residues. The full-length marine medaka IGF-II cDNA consists of a 639 bp ORF encoding 212 aa, a 109 bp 5’ UTR, and a 416 bp 3’ UTR. Homology comparison of the deduced aa sequences with other IGF-Is and IGF-IIs showed that these genes in marine medaka shared high structural homology with orthologs from other teleost as well as mammalian species, suggesting high conservation of IGFs throughout vertebrates. The IGF-I mRNA level increased following transfer of marine medaka from freshwater (FW) to SW, and the expression level was higher than that of the control group, which was maintained in FW. This significantly elevated IGF-I level was maintained throughout the experiment (14 days), suggesting that in marine medaka, IGF-I is deeply involved in the adaptation to abrupt salinity change. In contrast to IGF-I, the increased level of marine medaka IGF-II mRNA was only maintained for a short period, and quickly returned a level similar to that of the control group, suggesting that marine medaka IGF-II might be a gene that responds to acute stress or one that produces a supplemental protein to assist with the osmoregulatory function of IGF-I during an early phase of salinity change.
혈관내피세포에서 꽃송이버섯(Sparassis crispa) 소수성 추출물의 항혈관신생 활성
한장미,공소연,송재경,강예재,정혜진 한국식품과학회 2019 한국식품과학회지 Vol.51 No.2
Sparassis crispa is an edible mushroom that is distributed in Korea, Japan, Europe, and North America. It exerts various biological activities such as immunopotentiation, anti-diabetic, anti-cancer, and anti-inflammatory effects. Recently, we separated the health functional non-aqueous fraction from the chloroform extract of S. crispa (SCF4). In this study, we evaluated the antiangiogenic activity of SCF4 in human umbilical vein endothelial cells (HUVECs). SCF4 effectively inhibited vascular endothelial growth factor (VEGF)-induced cell growth at concentrations (5-25 μg/mL) showing no cytotoxic effects. SCF4 inhibited VEGF-induced invasiveness and tube formation ability, which are in vitro angiogenic features of HUVECs, in a dose-dependent manner. In addition, SCF4 markedly suppressed in vivo angiogenesis of chorioallantoic membrane from growing chick embryos without cytotoxicity. Furthermore, SCF4 downregulated the phosphorylation of VEGFR2, AKT, and ERK1/2, which are major angiogenic signal mediators. These results suggest that SCF4 inhibited angiogenesis by suppressing the VEGFR2 signaling pathways without cytotoxicity.
Introduction of bacterial and viral pathogens from imported ornamental finfish in South Korea
Hee Jae Choi,허준욱,조재범,박관하,정혜진,강예재 한국수산과학회 2019 Fisheries and Aquatic Sciences Vol.22 No.1
Background: Live fish import may lead to the unintended introduction of pathogens. We examined the monthly distribution of microbial pathogens in ornamental finfish imported into South Korea over a 6-month period. Results: Vibrio alginolyticus was detected in one lemon damsel in June and July; V. vulnificus was detected in one lemon damsel, one caerulean damsel, and one pearl-spot chromis and one ocellaris clownfish in July, April, and May, respectively; Photobacterium damselae was detected in one ocellaris clownfish and one caerulean damsel in June and July, respectively; V. anguillarum was detected in one pearl-spot chromis in February; V. harveyi was detected in one ocellaris clownfish and two mandarin fish in February and April, respectively; Yersinia ruckeri was detected in a pearlscale goldfish group in June and July and in two colored carp groups in July; and Lactococcus garvieae was detected in a lemon damsel group and a sutchi catfish group in July and May, respectively. European catfish virus, the only viral pathogen detected, was found in two sutchi catfish groups in May. Conclusion: This study is the first to identify pathogenic species and the presence or absence of pathogens (nonquarantine diseases) in imported ornamental finfish. These results demonstrate that various pathogens with the potential to harm indigenous fish populations can accompany ornamental finfish imported into South Korea.
삼종염(Potassium monopersulfate)을 주성분으로 하는 소독제의 주요 수산 병원체에 대한 살균소독 효과
최다연,최희재,조아현,서정우,박지원,나정현,이병윤,김준환,강예재 한국어병학회 2023 한국어병학회지 Vol.36 No.1
Infectious diseases are a major cause of death at the fish farm. Therefore, various disinfectants are used in aquaculture to sterilize pathogens. In this study, the disinfectant efficacy of triple salt was evaluated against Salmonella typhimurium and important aqua pathogens. In the case of bacteria under the conditions of distilled water (DW), hard water (HW), and organic matter (OM), S. typhimurium was inactivated with 0.56, 0.5 and 100 parts per thousands (ppt), V. parahaemolyticus (AHPND) was inactivated with 0.17, 0.13 and 20 ppt and V. anguillarum was inactivated with 0.25, 0.17 and 33.3 ppt. Also, in the case of the virus under the conditions DW, HW and OM, VHSV was inactivated with 0.1, 0.1 and 0.5 ppt and SVCV was inactivated with 0.5, 0.5 and 1 ppt. In addition, this study investigated the disinfection effect of triple salt against WSSV by bioassay using Litopenaeus vannamei. Investigating the antiviral effect of disinfectants under DW, HW, and OM conditions, WSSV was inactivated with 0.05, 0.05, 0.8 ppt. The results from this study showed that the triple salt was a highly effective disinfectant against aqua pathogens. Therefore, we can provide basic data for establishing the standards for use of triple salt in aquaculture.
Lee Ji-Hoon,Bae Jun Sung,Lee Chae Won,Yang Chan Yeong,Choi Ji-Sung,Choi Sang-Hoon,강예재,박관하 한국수산과학회 2020 Fisheries and Aquatic Sciences Vol.23 No.2
Background: Butaphosphan (BTP) has recently been introduced into the Korean aquaculture sector as a stressattenuating agent. In this study, a sensitive chemical analytical method was established for the detection of BTP in the olive flounder (Paralichthys olivaceus) tissues. Methods: Utilizing a method employing liquid chromatography coupled with tandem mass spectrometry (LC-MS/ MS), detection sensitivity, specificity, and precision were satisfactorily established. Temporal changes in the BTP plasma and muscle concentrations were assessed after a single intramuscular injection of BTP (50 and 150 mg/kg) to the olive flounder maintained at 13 °C or 22 °C. Results: High BTP plasma levels were achieved immediately after the injection, and the drug was rapidly eliminated. Additionally, plasma BTP levels were markedly dependent on the elimination rate, which, in turn, seemed dependent on the water temperature, with the drug elimination half-life and mean residence time significantly shorter at 22 °C than 13 °C. Overall, muscle BTP levels were markedly lower than the plasma levels. Notably, muscle levels were not influenced by water temperatures. Muscle BTP concentrations were used to estimate the necessary withdrawal period for drugs used in food fish, with BTP levels maintained far below the possible hazardous limit. Conclusions: In conclusion, the established LC-MS/MS method can be used for BTP residue detection with high sensitivity and reproducibility.
수온별 아질산 급성 노출에 따른 넙치, Paralichthys olivaceus의 혈액학적 성상 및 혈장성분의 영향
홍수민 ( Su-min Hong ),조아현 ( A-hyun Jo ),김다은 ( Da-eun Kim ),박연숙 ( Yeon-sook Park ),이혜성 ( Hye-sung Lee ),전유현 ( Yu-hyeon Jeon ),김석렬 ( Seok-ryel Kim ),김대희 ( Dae-hee Kim ),강예재 ( Yue Jai Kang ),김준환 ( Jun-hwan 한국어병학회 2021 한국어병학회지 Vol.34 No.2
Olive flounder (Paralichthys olivaceus) (Weight 110.9±17.1 g, length 22.3±1.2 cm) were exposed to waterborne nitrite at 0, 30, 60, 120, 240, 480 and 960 mg NO<sub>2</sub> <sup>-</sup>/L according to water temperature at 20℃ and 25℃ for 96 hours. The lethal concentration 50 (LC<sub>50</sub>) of olive flounder, P. olivaceus exposed to waterborne nitrite was 513.87 mg NO<sub>2</sub> <sup>-</sup>/L at 20℃ and 208.35 mg NO<sub>2</sub> <sup>-</sup>/L at 25℃, which means a significant difference in LC<sub>50</sub> by the water temperature. Hemoglobin and hematocrit were significantly decreased by waterborne nitrite exposure. The inorganic component, plasma calcium, was significantly decreased, and the organic components such as plasma glucose and cholesterol were significantly decreased showing a similar tendency with calcium. In enzymatic components, the AST and ALP were also significantly decreased by nitrite exposure. The results of this study indicate that exposure to nitrite can affect the survival and hematological physiology of P. olivaceus, and the effect of exposure to nitrite had a significant effect on nitrite toxicity depending on the water temperature.