HBV-trimera 동물모델을 통한 B형 간염 항체의 효능평가에 관한 연구

강영국(Young Kook Kang),장명희(Myeong Hee Jang),김근수(Keun-Soo Kim),오미숙(Mee Sook Oh),김남재(Nam Jae Kim),이병석(Byung Seok Lee),이은나(Eun Na Lee),김성주(Sung Joo Kim),류춘제(Chun Jeih Ryu),홍효정(Hyo Jeong Hong) 한국실험동물학회 2005 Laboratory Animal Research Vol.21 No.1

Humanized Balb/c mice (termed Trimera mice) conditioned by lethal total body irradiation and bone marrow transplantation from NOD/SCID mice have been described to support efficient engraftment of human tissues. To evaluate the efficacy of potential anti-HBV agents in vivo, we, here, describe the development of a mouse Trimera model for human hepatitis B virus (HBV) infection. HBV viremia was induced by transplantation of ex-vivo HBV-infected human liver fragments under the kidney capsule of Trimera mice (HBV-Trimera). The levels of HBV viremia were determined by measuring serum HBV DNA using polymerase chain reaction (PCR) at 10 days after liver transplantation. In order to evaluate the therapeutic potential of two humanized monoclonal antibodies specific to hepatitis B surface antigens, the HBV-Trimera mice were administrated intraperitoneally with anti-HBs antibody (HzSIII) or anti-preS1 antibody (AP301) at days 14 to 17 post-liver transplantation. Treatment of the HBV-Trimera mice with two anti-HBV humanized monoclonal antibodies (HzSIII, AP301) reduced the viral load in their sera in a dose-dependent manner, suggesting that the humanized antibodies will be useful in the prevention and treatment of HBV infection. These results suggest that the HBV-Trimera mice can be used as an animal models for evaluating therapeutic efficacy of anti-HBV agents.

누에의 에탄올 추출물이 체내 활성산소 생성에 미치는 영향

강영국(Young Kook Kang),최민주(Min Joo Choi),남상호(Sang Ho Nam) 한의병리학회 2014 동의생리병리학회지 Vol.28 No.4

동과추출물이 흰쥐의 기관 · 기관지 내 점액분비에 미치는 영향

강영국(Young Kook Kang) 한의병리학회 2016 동의생리병리학회지 Vol.30 No.5

흰쥐 뇌에서 monoamine oxidase 활성에 대한 인삼 물 추출물의 억제효과

강영국 ( Kang Young-kook ) 대전대학교 한의학연구소 2024 혜화보건의료학회지 Vol.3 No.1

Objectives: In this study, For investing the efficacy of ginseng on anti-stress, antidepressant and neuroprotective effects, the inhibitory effects of MAO-A, MAO-B, and total-MAO activities were investigated after long-term feeding of ginseng water extract (GWE) to rats. Methods: Experimental animals were divided into control group, GWE6, and GWE12 groups. Control group was fed the clean water, GWE6 and GWE12 groups were fed for 6 months and 12 months, respectively, by diluting GWE (25 mg/kg/day) in water. And activities of the enzymes were measured in the mitochondrial fraction of brain. Results: The activities of MAO-A, MAO-B, and T-MAO were inhibited by about 28% to 30%, 20% to 27%, and 14% to 30%, respectively, with feeding GWE to experimental animals. Conclusions: These results imply that ginseng can efficiently improve not only anti-stress, antidepressant, but also degenerative neurological brain disease. In addition, This is considered to be of great valuable as a basic experiment to reveal the efficacy of ginseng in more detail through the animal experimental model of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) induced Parkinson's disease.

애반딧불이(Luciola lateralis) 유충의 상륙에 미치는 수온의 영향

오홍식,강영국,남상호,Oh, Hong-Sik,Kang, Young-Kook,Nam, Sang-Ho 한국응용곤충학회 2009 한국응용곤충학회지 Vol.48 No.2

본 연구는 애반딧불이 유충의 상륙에 미치는 수온의 영향을 실내 외 조건에서 조사하였다. 실내 실험 결과에서 수온이 평균 19.3${\pm}$$0.3^{\circ}C$ 정온조건에서는 상륙 행동을 보이지 않았고, 20${\sim}$$22^{\circ}C$ 변온조건(평균 20.9${\pm}$$0.9^{\circ}C$)에서는 상륙을 시도하였지만 실제로 상륙은 이루어지지 않았다. 그러나 유충들은 평균 20.8${\pm}$$0.6^{\circ}C$ 정온조건에서는 가장 활발하게 유충의 상륙이 이루어졌다. 관찰 시점에 대한 조사는 실내 실험에서 유충의 상륙 빈도는 수온 20${\sim}$$21^{\circ}C$에서 전체의 63.6%로 높게 나타났다. 자연 서식처 실험에서 수온 19.8${\sim}$$21.7^{\circ}C$ 구간에서 상륙이 이루어졌으며, 상륙빈도수가 20${\sim}$$21^{\circ}C$에서 전체의 80.4%로 높게 나타났다. 서식처 실험에서는 실내 실험에서 유충의 상륙이 관찰되지 않았던 19${\sim}$$20^{\circ}C$구간에서도 유충의 상륙이 약간 관찰되었다. 실내 실험과 자연 서식처 실험의 조사일별에 대해 수온이 20${\sim}$$21^{\circ}C$에서 상륙빈도가 높게 나타났다. 실내 실험에서 유충의 상륙에서 성충의 출현까지 기간은 약 18일 소요되었으며 서식지 내에서 유충의 상륙부터 성충의 최초 출현 일과 최대 개체수 출현 일 사이의 기간 동안 기록된 수온의 구간은 19.6${\sim}$$21.8^{\circ}C$이었고 수온은 평균 20.4${\pm}$$2.3^{\circ}C$로 나타났다. 위의 결과를 보아 상륙에 요구되는 수온의 범위는 $20^{\circ}C$${\sim}$$21^{\circ}C$인 것으로 추정되며 가장 적정한 수온은 약 $21^{\circ}C$로 추정되었다. This study was conducted to investigate the relationship between the climbing up event of Luciola lateralis larvae and water temperature. In the laboratory condition, the larvae did not come out of water at constant temperature condition of 19.3${\pm}$$0.3^{\circ}C$ and tried to climb up at varying temperature condition of 20${\sim}$$22^{\circ}C$ (mean temperature 20.9${\pm}$$0.9^{\circ}C$) without success. However, they climbed up at constant temperature condition of 20.8${\pm}$$0.6^{\circ}C$. The frequency of the larval climbing up was highest as 63.6% at 20${\sim}$$21^{\circ}C$. The most larvae climbed up at approximately $21^{\circ}C$ of average daily water temperature, In the natural condition, the larvae climbed up at 19.8${\sim}$$21.7^{\circ}C$ and the frequency of the larval climbing up was high as 80.4% at 20${\sim}$$21^{\circ}C$. The larval climbing up was also little observed at 19${\sim}$$20^{\circ}C$ at which no larval climbing up was found in the laboratory experiment. No larvae attempted to climb up when water temperature was below $19^{\circ}C$ and above $22^{\circ}C$. It took 18 days from climbing up of larvae to appearance of adults. In its habitat, the range of water temperature required for larvae to climb up is assumed to be 19.6${\sim}$$21.8^{\circ}C$ and the most suitable water temperature may be 20.4${\pm}$$2.3^{\circ}C$. Therefore, the range of water temperature required for larvae to climb up is assumed to be 19.8${\sim}$$21.7^{\circ}C$, and the suitable water temperature range for larvae may be 20${\sim}$$21^{\circ}C$, and the most suitable temperature is thought to be $21^{\circ}C$.

애반딧불이(Luciola lateralis)의 생태학적 특성

오홍식,강영국,남상호,Oh, Hong-Sik,Kang, Young-Kook,Nam, Sang-Ho 한국응용곤충학회 2009 한국응용곤충학회지 Vol.48 No.2

애반딧불이의 유충상륙은 약 5.3일이 소요되었고 고치방을 만든 후 약 6.6일에 번데기로 변태하였다. 고치방의 크기는 길이 10.1 mm, 폭 4.7 mm, 벽두께 1.3 mm이었다. 번데기는 약 10.5일이 소요되었다. 성충이 되고 고치방 속에 약 6.8일 후에 출현하였다. 성충의 출현은 PM. 9시를 전후로 최대였고 PM. 10시에 감소하였다. 성충출현의 최적지온은 $23.4^{\circ}C$였다. 실내개체군(Lab-type)의 암컷 및 수컷과 야외개체군(Nat-type)의 수컷은 수명이 비슷하였고, Nat-type의 산란 수는 200${\sim}$400립 구간을 형성하였다. 단지, Nat-type의 암컷은 수명이 짧았으며, 산란 횟수와 산란 수는 적었다. 산란횟수에서 야외개체군은 2회와 실내개체군은 4회로 차이를 보이는 것은 야외개체군은 시료 채집 이전에 산란했을 가능성을 배제할 수 없다. The Luciola lateralis larva took 5.3 days from climbing on the land to the pupal cocoon formation. It took 6.6 days for a larva to eventually transform to a pupa after building a pupal cocoon. The size of pupal cocoon was 10.1 mm in length, 4.7 mm in width and its wall thickness was 1.3 mm. The mean pupal period was 10.5 days. The adult stayed 6.8 days in the pupal cocoon before escaping the cocoon. The peak adult emergence appeared around 9 p.m., and decreased after 10 p.m. The optimal soil temperature for emergence was $23.4^{\circ}C$. The female adult of the natural population (Nat-type) lived shorter, laid fewer eggs, and the oviposition frequency was fewer than that of the Lab-type individuals. However, a few individuals from the natural population laid 200-400 eggs. The less number of oviposition in the natural population may be due to the fact that the female adults might lay eggs before the collection for the experiment.

흡연자와 비흡연자의 타액에서 코티닌 정량 및 ETS 노출량 평가

임흥빈,강영국,손형옥,이영구,문자영,이동욱 충북대학교 엽연초연구소 2001 煙草硏究 Vol.18 No.-

This study was conducted to measure and compare the salivary cotinine concentration between smokers and nonsmokers, and to investigate the effect on the exposure to environmental tobacco smoke(ETS) with housewives, living on an apartment of Korea. Saliva samples were taken from all volunteers and salivary cotinine was analyzed by a developed radioimmunoassay(RIA) method. The median salivary cotinine levels were 52.8±12.6 ng/ml and 44.6± 9.7 ng/ml in male smokers and in female ones, respectively, and they were 4.3±2.1 ng/ml and 2.9±3.1 ng/ml in male nonsmokers and in female ones, respectively. In addition, average salivary cotinine levels were 3.8±2.7 ng/ml in housewives from smoking homes and 2.4±2.4ng/ml in ones from nonsmoking homes. Results obtained from the analysis of salivary cotinine demonstrate that smokers had a significantly higher concentration of salivary cotinine than nonsmoker. But, there was no significant difference in the salivary cotinine concentration for housewives living in apartment between with smoking husband and with nonsmoking husband.