동면에 관한 연구 - 혈중 Insulin 농도의 변화 -

강복순,강두희,Kang, Bok-Soon,Kang, Doo-Hee 대한생리학회 1972 대한생리학회지 Vol.6 No.1

Physiological changes in hibernating animals (Manchurian Chipmunk and Hamster) were studied during various phases of the hibernation. The results obtained are summarized as follows. 1. Rectal temperature was declined markedly during hibernation, whereas it remained constant even in exposure of the animals to 0 C environment if the animals were not prepared for hibernation. As body temperature was lowered, heart rate was also markedly reduced as well as respiration and blood pressure. 2. The electrical activity recorded from cortical structure was characterized by replacement of fast wave activity by slow wave activity as body temperature was lowered and became flat at body temperature below 20C. 3. Blood glucose level showed no clear seasonal variations. However, the glycogen contents of the liver were markedly greater during the cold seasons than during the warm seasons. 4. Blood levels of insulin showed no clear seasonal variations.

가야사 접근성 향상을 위한 게이미피케이션 적용 개발 연구 및 추진 사례

강복순 한국지역정보개발원(구 자치정보화조합) 2019 지역정보화 Vol.114 No.-

한냉적응이 Adenyl Cyclase Activity에 미치는 영향

강복순,이상호,강두희,Kang, Bok-Soon,Lee, Sang-Ho,Kang, Doo-Hee 대한생리학회 1974 대한생리학회지 Vol.8 No.2

The object of this research is aimed to determine the activity of adenyl cyclase in both skeletal muscle sarcolemma and fat cell ghost of epididymal adipose tissue isolated from rats exposed to cold for various length of time in an attempt to evaluate whether the tissue sensitivity to catecholamine is increased when rats are exposed to cold for long periods of time Methods: a)Animals: Albino rats ranging in weight from 150 to 200 gm were used throughout this study. For experimental purposes, the rats are divided into two groups: experimental animals were place4 in a cold room at $4^{\circ}C$, controls being kept at $25^{\circ}C$. At the end of 2, 4, 6, 12, and 16 weeks. exposure to cold the rats were used to measure the adenyl cyclase activity. b) Isolation of plasma membrane from skeletal muscle and adipose tissue: The Plasma membrane of skeletal muscle from hind limbs of rats are prepared by the method employed by Rosenthal et at. and fat cell ghost of epididymal adipose tissue of rats by the method employed by Rodbell. c) Adenyl cyclase assay: Adenyl cyclase activity were measured by the method employed by Marinetti et al. Briefly, plasma membrane was incubated with $3^H-ATP$, various amount of noradrenaline and other incubation mixture at $37^{\circ}C$ for 20 minutes. After stopping the enzyme reaction by immersion in boiling water, carrier 3',5'-AMP was added to the system as a marker and $100\;{\mu}1$ aliquots of incubation mixture were pipetted on $20{\time}20$ Whatman No. 3 MM filter paper for one dimensional chromatography. The cyclic AMP spots were cut off and placed in counting vials containing 10ml of Bray's scintillation cocktail. Radioactivity was determined with a Packard Tri-Carb liquid scintillation counter. The enzyme activity is expressed as nanomoles of cyclic AMP produced per mg of membrane per hour. Result: 1. Average adenyl cyclase activity in the plasma membrane of skeletal muscle before and after noradrenaline administration was significantly higher in the cold-exposed rats as compared to the control. Continuous exposure to cold Produced an increased adenyl cyclase activity before and after noradrenaline administration. Adenyl cyclase activity reached peak levels at the 6 weeks exposure to told and level of adenyl cyclase activity remained high. Noradrenaline administration to the incubation medium induced a significant increase in adenyl cyclase activity and the degree of stimulation were proportional to the hormonal concentration But the rate of inclement in adenyl cyclase activity by noradreasline was the same in both groups. 2. Adenyl cyclase activity in fat cell ghost between cold exposed and control rats showed no significant differences before and after noradreualine administration. In summary, it can be concluded that cold adaptation give rise an increased activity of adenyl cyclase in plasma membrane of skeletal muscle in rats.

Hypoxia에 의한 혈관이완과 수축의 기전에 관한 연구

강복순,이영호 대한순환기학회 1998 Korean Circulation Journal Vol.28 No.12

의식의 생리

강복순 연세대학교 의과대학 1984 연세의대논문집 Vol.17 No.1

호흡 및 근육계통

강복순,강두희 대한의학협회 1976 대한의학협회지 Vol.19 No.2

사람적혈구막에서의 Calcium과 Suramin의 상호작용

강복순,강두희 대한생리학회 1976 대한생리학회지 Vol.10 No.1

인삼이 적혈구세포의 해당과정 및 막 투과도에 미치는 영향

강복순(Kang, Bok-Soon),한경희(Han, Kyung-Hee) 대한생리학회 1983 대한생리학회지 Vol.17 No.2

Red cell glycolytic intermediates, metabolites and metabolic ratios were studied. Glycolytic intermediates were measured in neutralized perchloric acid extracts of red cell suspensions after 3 hr incubation at 37℃ in the presence and absence of saponin. Adenosine triphosphate(ATP), adenosine diphosphate(ADP), pyruvate and lactate were measured by enzymatic procedures involving stoichiometric oxidation or reduction of a pyridine nucleotide. Glucose was determined using glucose oxidase after zinc hydroxide extraction. The redox state was calculated from the lactate dehydrogenase equilibrium. Adenosine triphosphatase activity(ATPase) was measured by determining the amount of phosphate released from ATP by washed erythrocyte membranes(ghost) during 20 min. incubation. Both total hydrolysis and the amount of hydrolysis that occured in the presence of ouabain were measured. The second measurement yields Mg-ATPase and represents nonspecific ATPase activity of the membranes. The difference between total and Mg-ATPase activity can be attributed to Na-K-ATPase. For the measurement of sodium fluxes, human erythrocytes were preincubated in ²²Na for 3 hr at 37℃, washed and suspended in a tracer-free medium. The amount of ²²Na transported out of cells at any time was determined by analysis of supernatant samples taken at various time after addition of the labeled cells to isotope-free medium. The cells and medium were separated and the radioactivity appearing in the medium was measured. From the total radioactivity in the suspension and the radioactivity appearing in the medium at known time, the rate constant for sodium release was computed. The results are summarized as follows: 1) ATP and ATP/ADP were found to increase at every concentration of saponin tested whereas ADP declined at every cone. of saponin. The increase in pyruvate and lactate were observed at every cone, of saponin and thus NAD⁺/NADH computed from pyruvate/lactate also increased. Glucose utilization was stimulated by saponin. 2) Na⁺-K⁺-ATPase activities showed a biphasic response to saponin, first increasing in lower concentration and then decreasing in higher concentration of saponin. 3) The efflux of sodium was significantly increased by saponin in the range of 5 to 10 mg%. The stimulatory effect of saponin on the rate constants for active(ouabain-sensitive) sodium efflux was inhibited by addition of ouabain.

한국 해녀에서 잠수복 착용이 작업시간 및 열대사에 미치는 영향

강두희,강복순,이중우,김희중,Kang, Doo-Hee,Kang, Bok-Soon,Lee, Joong-Woo,Kim, Hee-Joong 대한생리학회 1976 대한생리학회지 Vol.10 No.1

Rectal temperature, skin temperature and oxygen consumption were measured in five women divers during diving under two conditions a) wearing a cotton bathing suit and b) wearing a diving suit and the following results were obtained. 1. The duration of the diving period was 30 min with the cotton bathing suit whereas it was above 120 min with the diving suit. 2. The average rectal temperature fell to $34.5^{\circ}C$ after 30 min of diving with the cotton bathing suit. On the other hand, with the diving suit, immediately after submersion, the rectal temperature rose about $3^{\circ}C$ and remained at about $39^{\circ}C$ throughout the diving period of 120 min. 3. Average mean skin temperature during diving was $11^{\circ}C$ and $21^{\circ}C$ with cotton bathing suit and with the diving suit, respectively. The mean body temperature fell to $25^{\circ}C$ with the cotton bathing suit while it reached $32^{\circ}C$ with the diving suit at the end of each diving period. 4. Oxygen consumption during diving was three fold of the pre-dive level with the cotton bathing suit but it was two fold with the diving suit. 5. Total extra heat loss was 323 kcal during 120 min of diving with the diving suit. On the contrary, with the cotton bathing suit, it was 528 kcal for 30 min, These results may suggest that the prolongation of the diving period with the diving suit is primarily due to maintenance of core temperature at more or less physiological level as a result of reduction in conductive heat loss from the body surface to the water and the Preferential distribution of blood in the core area.

기니픽의 회장평활근에서 NANC 신경전달물질의 작용기전

김종훈,강복순,이영호,Kim, Jong-Hoon,Kang, Bok-Soon,Lee, Young-Ho 대한약리학회 1997 The Korean Journal of Physiology & Pharmacology Vol.1 No.6

The relaxation induced by stimulation of the inhibitory non-adrenergic, non-cholinergic (iNANC) nerve is mediated by the release of iNANC neurotransmitters such as nitric oxide (NO), vasoactive intestinal peptide (VIP) and adenosine triphosphate (ATP). The mechanisms of NO, VIP or ATP-induced relaxation have been partly determined in previous studies, but the detailed mechanism remains unknown. We tried to identify the nature of iNANC neurotransmitters in the smooth muscle of guinea pig ileum and to determine the mechanism of the inhibitory effect of nitric oxide. We measured the effect of NO-donors VIP and ATP on the intracellular $Ca^{2+}$ concentration$([Ca^{2+}]_i)$, by means of a fluorescence dye(fura 2) and tension simultaneously in the isolated guinea pig ileal smooth muscle. Following are the results obtained. 1. Sodium nitroprusside $(SNP:10^{-5}\;M)$ or S -nitro-N-acetyl-penicillamine $(SNP:10^{-5}\;M)$ decreased resting $[Ca^{2+}]_i$ I and tension of muscle. SNP or SNAP also inhibited rhythmic oscillation of $[Ca^{2+}]_i$ and tension. In 40mM $K^+$ solution or carbachol ($(CCh:10^{-6}\;M)$-induced precontracted muscle, SNP decreased muscle tension. VIP did not change $[Ca^{2+}]_i$ and tension in the resting or precontracted muscle, but ATP increased resting $[Ca^{2+}]_i$ and tension in the resting muscle. 2. 1H-[1,2,4]oxadiazol(4,3-a)quinoxalin-1-one $(ODQ:1\;{\mu}M)$, a specific inhibitor of soluble guanylate cyclase, limited the inhibitory effect of SNP 3. Glibenclamide $(10\;{\mu}M)$, a blocker of $K_{ATP}$ channel, and 4-aminopyridine (4-AP:5 mM), a blocker of delayed rectifier K channel, apamin $(0.1\;{\mu}M)$, a blocker of small conductance $K_{Ca}$ channel had no effect on the inhibitory effect of SNP. Iberiotoxin $(0.1\;{\mu}M)$, a blocker of large conductance $K_{Ca}$ channel, significantly increased the resting $[Ca^{2+}]_i$, and tension, and limited the inhibitory effect of SNP. 4. Nifedipine $(1\;{\mu}M)$ or elimination of external $Ca^{2+}$ decreased not only resting $[Ca^{2+}]_i$ and tension but also oscillation of $[Ca^{2+}]_i$ and tension. Ryanodine $(5\;{\mu}M)$ and cyclopiazonic acid $(10\;{\mu}M)$ decreased oscillation of $[Ca^{2+}]_i$ and tension. 5. SNP decreased $Ca^{2+}$ sensitivity of contractile protein. In conclusion, these results suggest that 1) NO is an inhibitory neurotransmitter in the guinea pig ileum, 2) the inhibitory effect of SNP on the $[Ca^{2+}]_i$ and tension of the muscle is due to a decrease in $[Ca^{2+}]_i$ by activation of the large conductance $K_{Ca}$ channel and a decrease in the sensitivity of contractile elements to $Ca^{2+}$ through activation of G-kinase.