흰쥐 간의 Aryl hydrocarbon hydroxylase 에 대한 인삼 추출물의 선택적 효과

선우양일,박기현 ( Yang Il Sun Woo,Ki Hyun Park ) 생화학분자생물학회 1984 BMB Reports Vol.17 No.4

Many carcinogens of polycyclic aromatic hydrocarbons including BP are biotransformed by aryl hydrocarbon hydroxylase (AHHase, EC 1. 14. 14. 1) found in human and animal tissues into reactive intermediates such as epoxides. AHHase is a component of cytochrome P-450 (P-450) isozymes, and the P-450-containing enzyme system of liver microsome is remarkably versatile in the types of chemical reactions it catalyzes and in its choice of substrates. Accordingly, to evaluate the effects of ginseng extracts on AHHase activity, we treated intraperitoneally Sprague-Dawley rats (male, 160-180 g) with phenobarbital (Pb) or 3-methylcholanthrene (3-MC) as inducers of P-450. When the methanol or pet. ether extract of ginseng was added in hepatic microsome separated from rat untreated with chemical, AHHase activities in the both cases were sharply inhibited. Also, the changes of AHHase activities from microsome treated Pb were similar observations such as rats untreated. The AHHase activities from microsome treated with 3-MC as a potent inducer of P-450 were exhibited the opposite results by methanol extract as compared to that of untreated or Pb-treated rats, whereas pet. ether extract had no any effects for AHHase activities. Water extract did not have any effect such as results evaluated from untreated or Pb-treated rats. These results demonstrate that the activating and inhibiting effects of pet. ether or methanol extract depend on the isozyme type of P-450 induced with Pb or 3-MC.

들깻잎에서 동정한 Phytol의 항암 및 면역활성증강 효과

선우양일,류태형,박건영,이숙희,장명웅,김광혁 東亞大學校附設基礎科學硏究所 1993 基礎科學硏究論文集 Vol.10 No.1

Several studies have shown that extracts from yellow-green vegetables reveal antitumor activities. In the present study we investigated the effect of phytol in order to elucidate the immunological mechanism of antitumor activity of this substance. The results obtained from the experiment as follows : 1) Phytol showed cytotoxic effect on sarcoma 180 cells in vitro. 2) When phytol was injected into the peritoneal cavity of mice transplanted with sarcoma 180 cells, the average survival time(24.0 days) tended to increase as compared with the nontreated control(19.2 days). 3) When sarcoma 180 cells were injected subcutaneously into the right groin of mice and then phytol was injected into the peritoneal cavity the tumor inhibition ratio was 33%. 4) The natural killer(NK) cell activity was significantly augmented by phytol in vitro and in vivo. Similar augmentations of NK cell activity were obtained with culture supernatants of phytol exposed spleen cells and peripheral blood mononuclear cells. 5) Phytol on the macrophage from peritoneal cavity showed a higher effectiveness in vivo than in vitro. These results indicate that phytol shows the inhibitory effect for growth of sarcoma 180 cells in vitro, also it can augment macrophage and NK cell activities in vivo.

출아효모의 세포주기동안 DNA 상해에 의한 발현 유도에 미치는 DPB11 유전자의 영향

선우양일,임선희,배호정,김중현,김은아,김승일,김수현,박정은,김재우 한국미생물학회 2002 미생물학회지 Vol.38 No.2

S기 checkpoint기작은 DNA복제 저해나 DNA상해 등에 반응하여, S기 세포주기 정지를 일으키거나 상해 회복에 관련된 유전자들의 전사가 유도됨으로서 진핵세포에서의 유전적인 안정성을 유지한다. 이러한 반응에 대한것ba11 변이주의 결손을 확인하기 위해서, nPB11 (DNA polymerase B possible subunit)유전자의 과다발현 효과에 대해 조사하고, HU (Hydroxyurea)와 MMS (Methyl methanesulfonate)에 대한 감수성 및 DNA상해 물질에 의한 RNR3 (Ribonulectide reductase) mRNA의 전사 유도를 조사하였다. RNR3 mRNA의 전사는 DNA합성 저해에 의해 발생한 스트레스나 화학물질에 의한 직접적 인 DNA상해 등에 의해 유도되어진다. 그 결과, dpb11-1변이주는 DNA상해 물질에 감수성을 나타내었고, RNR3 mRNA전사유도 또한 야생형 균주에 비해 약 40% 정도 감소를 나타내었다. 더욱이 dpb2-1 균주에서도 이와 동일한 결과를 얻었다. 그러므로 DPB2와 DPB11 유전자는 복제에 대한 sensor로서, 복제 정지 요인에 대한 세포주기 반응과 전사 조절에 모두 작용하는 것으로 사료된다. The S-phase checkpoint mechanisms response to DNA damage or inhibition of DNA replication for maintenance of genetic stability in eukaryotic cells. These roles include cell cycle control arrest at S-phase and Iranscriptional induction of repair genes. To characterize the defects of dpbll mutant for both these responses, we examined the over-expression effect of DPBll gene, the sensitivity to HU, MMS, and the transcriptional pattern by DNA damage agent for RNRS mRNA. RNRS transcript is induced in response to a wide variety of agents that either damage D7A directly through chemical modification or induce stress by blocking DNA synthesis. As results, dpbll-1 cells are sensitive to DNA damage agents and the level of RNR3 mRNA is reduced approximately 40% than wild type cells. Moreover, we found the same results in dpb2-1 cells. Therefore, we propose that DPB2 and DPBll act as a sensor of replication that coordinates the transcriptional and cell cycle responses to replication blocks.

고정화된 Anabaena azollae 세포에 의한 질소와 인의 제거

선우양일,안태진,김광,박승조,박인호 동아대학교 환경문제연구소 1992 硏究報告 Vol.15 No.2

Anabaena azollae cells immobilized in cigarette filter were used with the removal of nitrate and phosphorus. The reduction of nitrate in the immobilized cells was increased according to the dose of treatment. The immobilized cells eliminated about 30% of the total phosphorus in the medium. These results indicate the possibility that the bioreactor with A. azolae cells immobilized in cigarette filter is available of biological treatment of wastewater.

Carboxymethyl chitosan의 항균성과 항곰팡이성

선우양일,박현숙,탁연수,최윤정,김광 동아대학교 환경문제연구소 1997 硏究報告 Vol.20 No.1

Chitin was converted to chitosan by deacetylation, and then carboxymethyl chitosan(CM-chitosan)was obtained by carboxymethlation of chitosan. The antibacterial activity was estimated using the miminum inhibitory concentration (MICwt%) assay. MICwt% of Bacillus subtilis, Escherichia coli, Pseudomonas aeruginosa, candida albican and Staphylococcus aureus were 0.5, 0.5, 0.25, 0.25, 0.75 respectively. CM-chitosan was tested for antifungal antivity which is measured the inhibition of hyphal extension of the fungi. MIC_(50)wt% of CM-chitosan was shown half growth of the experimental over control group. MIC_(50)wt% of Penicillium citrinum, Myrothecium verrucaria and Verticillium albo-atrum were 0.5, and Gliocladium virens was 0.75, respectively. These results showed that CM-chitosan had the high inhibitory effect on the growth of the bacteria and fungi.

환경성 유해요인이 유전물질과 세포활성에 미치는 영향. : Ⅱ. 돌연변이원에 의한 DNA 회복합성 , 염색체이상 및 자매염색분체 교환 Ⅱ. Mutagen Induced DNA Repair Synthesis , Chromosome Aberrtions and Sister Chromatid Exchanges

선우양일,신은주,엄경일,이천복 한국유전학회 1987 Genes & Genomics Vol.9 No.2

The effects of DNA polymerase α inhibitor aphidicolin (APC) on ethyl methanesulfonate (EMS)- or bleomycin (BLM)- induced repair of DNA damage, chromosome aberrations and sister chromatid exchange (SCE) were investigated in Chinese hamster ovary (CHO) cells. EMS induced DNA single-strand breaks, unscheduled DNA synthesis, chromosome aberrations and SCEs. The post-treatment with APC inhibited repair of DNA damage and exhibited synergistic effect on chromosome aberrations induced by EMS, but APC did not affect on EMS-induced SCEs. The DNA single-strand breaks, unscheduled DNA synthesis and frequencies of chromosome aberrations induced by BLM were linearly increased according to increment of treated concentration, but SCEs induced by BLM was shown with only a few increment. There was no effect of APC on the repair of DNA damage induced by BLM, whereas APC showed a synergistic effect on chromosome aberrations induced by BLM. These results suggested that there seemed to be no relationship among DNA repair, chromosome aberrations and SCE, so that the respective mechanisms were involved in the production of these three damage types.

밀기울배지를 이용한 Bacillus macerans의 Cyclodextrin Glucanotransferase 생산과 효소특성

선우양일(Yang-Il Sunwoo),안태진(Tae-Jin Ahn),김동환(Dong-Hong Kim),박인호(In-Ho Park),채기수(Ki-Su Chae),김광(Kwang Kim) 한국생물공학회 1994 KSBB Journal Vol.9 No.5

Butylatedhydroxyanisole과 Flavone에 의한 연기성분의 생체내 활성억제에 관한 연구

선우양일,박기현 한국연초학회 1980 한국연초학회지 Vol.2 No.2

연기성분의 생체내 활성을 억제시키기 위하여 Wistar male rat에 담배연기 응축물과 이의 중성분획을 5mg/kg, 10mg/kg 각각 복강주사한후 경시적으로 Lactic acid dehydrogenase(LDH) activity를 대조군과 비교하여 유해정도를 검토하였다. 그리고 연기 성분의 활성 억제물질로 선정한 flavone과 butylatedhydroxyanisole(BHA)를 각각 1mg/1kg처리하여 그 활성억제 정도를 관찰한 결과 flavone처리에 의한 연기응축물의 LDH activity는 전반적으로 현저한 감소현상을 보였으며 12시간후에는 641 unit로서 처리하지 않았을 때의 1040unit에 비하여 괄목할만한 결과를 나타냈다. 그러나 BHA처리에 의한 결과는 flavone보다 다소 저조한 현상을 보였다. 따라서 flavone은 연기 응축물의 활성억제에 보다 효과적인 것으로 생각된다. 한전 중성분획처리의 경우는 flavone에 비하여 BHA가 LDH활성도 억제헌상을 보였는데 주사후 6∼18시간사이에 LDH활성도가 100∼200unit의 감소현상을 나타냈다. 그러므로 중성분획에는 BHA가 바람직한 억제물질로 사료된다. The inhibition effect of flavone and butylatedhydroxyanisole (BHA) on the microsomal activation of Cigarette Smoke Condensate (CSC) or its Neutral Portion (NP) was investigated in Rat. The activities of Latic acid dehydrogenase (LDH) in serum was measured in the time intervals of 3, 6, 12, 18, 24 and 30 hr, respectively, after the injection (ip) of CSC (5mg/kg) or NP (10mg/kg) to Wistar male rat. Flavone (1mg/kg) and BHA (1mg/kg) were injected along with CSC or NP. The significant enhancement of the LDH activity in serum was observed in both cases of rats treated with CSC and NP. A drastic decrease of LDH activity from 1040 unit to 641 unit was observed after 12 hours of injection of CSC along with flavone. In contrast with the case of flavone, BHA reduced the enzyme activity from 825 unit to 652 unit at the same condition of flavone. Therefore, flavone can be considered to be a better inhibitor on action of CSC in vivo.

밀기울배지를 이용한 Bacillus macerans Cyclodextrin Glucanotransferase 생산과 효소 특성

선우양일,안태진,김동환,박인호,채기수,김광 東亞大學校 附設 基礎科學硏究所 1994 基礎科學硏究論文集 Vol.11 No.1

Bacillus macerans cyclodextrin glucanotransferase(CGTase)를 전분흡착법과 DEAE-cellulose 칼럼 크로마토그래피로 정제하였다. 효소의 분자량은 67,000이었고 monomer였다. 정제된 효소는 전분을 α-β- , ?? 전환시켰으며, CD생성비율은 각각 1:1.68:0.32 였다. α-CD와 D-glucose의 coupling반응 초기에는 maltohexose가 주로 생성되었고, 그 후에 다른 oligosaccharide 들이 생성되었다. α-CD의 가수분해반응 초기에는 주로 maltotetrose가 생성되었고 그 이후에는 소량의 다른 oligosaccharide들이 생성되었다. 정제된 효소의 좋은 기질인 maltotrisoe로부터 maltosyl이나 D-glucopyranosyl group이 전이될 수 있는데, 본 연구에서는 D-glucosyl transfer가 우세하다. Bacillus macerans cyclodextrin glucanotransferase(EC 2.4.1.19: 1, 4-α-D-Glucan 4-α-D(1, 4-α-glucano)-transferase, CGTase) was purified by the technique of starch adsorption and DEAE-cellulose column chromatography. The molecular weight of the enzyme was 67,000, consisting of a subunit. The enzyme converted starch into α-β- and ?? in the relative amounts of 1:1.68:0.32, respectively. In the early reaction period, maltohexose was formed mainly by the coupling reaction of α-CD with D-glucose and then other oligosaccharides. Maltotetrose was formed mainly from α-CD in the initial stage of hydrolysis of the enzyme and then small amount of other oligosaccharides. Maltotriose was a good substrate for the enzyme and maltosyl or D-glucopyranosyl group can be transfered from this sugar. In this work, D-glucosyl transfer was prefered.

포유동물세포에 있어 자외선에 의한 절제회복에 미치는 수종 알킬화제의 영향

선우양일,박상대,홍승환,성노현 한국유전학회 1983 Genes & Genomics Vol.5 No.1

The present investigation determined how two different types of DNA damaging agents interact in inducing DNA single strand breaks and excision repair in CHO cells using UV-light and MMS. The results showed that strand breaks induced try UV-light were in low level when compared to those by MMS. Treatment with ara-C plus hydroxyurea, however, effectively accumulated DNA single strand breaks induced by UV-light, but not by MMS. When these cells were treated with UV-light and MMS concomitantly, UV-induced excision repair was inhibited by MMS, but the amounts of DNA single strand breaks were additive. These results suggested that a protein complex involved in UV-induced excision repair may be inactivated by MMS in excision-reinsertion step.