http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Identification of a novel candidate gene for rolled leaf in rice
고희종,이윤경,우미옥,이동령,이길응,김백기 한국유전학회 2016 Genes & Genomics Vol.38 No.11
A semi-narrow and adaxially rolled leaf mutant, rl15(t), was induced from Korean japonica rice cultivar Ilpum by chemical mutagenesis using ethyl methanesulfonate. We characterized the mutant and identified the novel gene causing the mutant phenotype. Cytological analysis of mutant leaves indicated that the adaxial leafrolling phenotype is due to the reduced size and number of bulliform cells in the mutant. Genetic analysis showed that the rolled leaf trait is controlled by a single recessive gene, designated rl15(t). Using an F2 mapping population generated from a cross between Milyang23 and the mutant, we mapped the candidate region to a 174 kb interval on the long arm of chromosome 1 near the centromeric region. Through whole genome sequencing in bulk and MutMap analysis, we identified the causal SNP within the candidate region. The results of RT-PCR analysis indicated that a splicing error occurred due to a base change from G to A at the beginning of the fifth intron of LOC_Os01g37837, which encodes a putative seryl-tRNA synthetase, resulting in the mutant phenotype. Further study of the rl15(t) gene will facilitate analysis of leaf architecture and morphogenesis in rice plants.
高熙宗,朴淳直,元容在,許文會 韓國作物學會 1993 한국작물학회지 Vol.38 No.6
화청벼 에서 유기된 초거대배 돌연변리계통을 육성하고 그의 미립 외형특성, 단백질함량 및 아미노산 조성, 지질함량 및 지방산 조성, 비타민 함량등의 영양특성 및 작물학적 특성을 원품종과 대비하여 조사하였다 그 결과는 다음과 같다. 1. 초거대배 변리계통의 현미 외형크기는 원품종과 차이가 없었으나, 1000립중은 원품종의 74.5%이었고, 1립당 배의 크기는 건물중으로 322%이었다. 2. 현미의 단백질함량은 8.99%로 화청벼의 7.39%에 비해 높았고, 저장단백질의 조성은 차이가 없었다. 아미노산조성에서는 현미 전체로 볼 때 lysine 함량이 월등히 증대되었고, methionine, serine, ,tyrosine 함량이 다소 감소되었다. 3. 현미의 지질함량은 5.7%로 원품종의 3.8%에 비해 1.5배 증가하였다. 지방산 중 팔미틴 산 함량은 증가하였고, 리놀산 함량은 감소하였다. 4. 배의 비타민 B1,B2 , niacin함량에서 초거대배미와 원품종과는 차이가 없었으며, α-tocopherol 함량은 감소하였으나, 현미 전체로 보면B1,B2 , α-tocopherol 함량이 월등히 증가하였다. 5. 초거대배미는 고영양미로서의 이용가치가 클것으로 판단되었다. 6. 초거대배 변이계통(M4)들의 정조수량은 원품종인 화청벼(556kg/10a)에 비해 69.2~78.8% 정도이었다. 생육형질의 변이계수로 보아 대부분 계통들이 고정된 것으로 나타났다. Super-giant-embryo mutant line (Hwacheong-ges ) with high nutritional quality was developed from Hwacheongbyeo, a Korean Japonica cultivar. Grain weight of brown rice of Hwacheong-ges line was lighter than of Hwacheongbyeo. However, embryo dry weight of the line was 3.22 times heavier than that of Hwacheongbyeo on the single grain base. Hwacheong-ges line showed higher protein(8.99%) than the original variety(7.39%), without changes in storage protein. In amino acid composition, lysine content was greatly increased in the mutant, while the contents of methionine, serine and tyrosine were slightly decreased as compared with the original one. Lipid content of Hwacheong-ges line was 1.5 times higher compared with that of Hwacheongbyeo. The contents of Vitamin Bl, B2, and E(α -tocopherol) were also greatly increased in Hwacheong-ges line. The grain yields of the mutant lines ranged from 69.2% to 78.8% compared with that of Hwacheongbyeo, 556kg /10a. Most of the mutant lines of M4 generation were homogeneous for growth characters, and some of them seemed to be readily applicable for farmer's field.