Acquired Tufted Angioma 1예

문기찬,성경제,지두현,김현수,고재경 울산대학교 의과대학 1995 울산의대학술지 Vol.4 No.1

Tufted angioma was first described by Wilson Jones in 1976. This is a rare vascular tumor usually appears in early childhood, having a predilection for neck, upper chest and shoulder, and most commonly appears as dull red to red-brown patches and plaques. We report a case of acquired tufted angioma in an adult presenting as multiple, discrete, reddish papules within macular patch on right chest and showing a good response to dye laser therapy. This is an unusual case which merits consideration.

소의 대뇌 DeoxyribonucleaseⅠ 억제물질의 분리와 성상에 대한 연구

양승재,이상훈,이용성,고재경 한양대학교 의과대학 1990 한양의대 학술지 Vol.10 No.2

Deoxyribonuclease I (DNase I) inhibitor of protein nature was isolated from bovine brain and was characterized to clarify the regulatory mechanisms of DNase I activity in vivo. 1. Two kinds of DNase I inhibitor (peak I and peak II) were isolated and purified to 15 fold for peak I and 26 fold for peak II by centrifugation, ammonium sulfate fractionation, gel filtration and DEAE-cellulose chromatography. The molecular weights of peak I and peak II inhibitors were estimated to be 160,000 and 42,000 dalton, respectively. 2. The maximum inhibitory activities of both peak I and peak II inhibitors were observed at pH 7.5 and Mg²+ ion increased their inhibitory activities whereas Ca²+ and Mn²+ showed little effects on their inhibitory activities. The purified peaks I and II inhibitors also revealed specific inhibitory activities to DNase I and did not exert any inhibitory effecs on pancreatic RNase. 3. Peak I inhibitor protein was more stable than peak II inhibitor protein to heat inactivation. Only 8% of peak I activity disappeared by heat pretreatment at 45℃ for 10 minutes instead of 65% in case of peak II. 4. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis(SDS-PAGE) patterns for peak I and peak II inhibitor proteins revealed that the major protein peaks were located at the same position and molecular weight of the protein was estimated to be 42,000 dalton. These results suggest the presence of a heat-stable DNase I inhibitor of protein nature in bovine brain which may consist of 4 monomeric units of actin, an well known DNase I inhibitor.

횡문근육종 환아의 혈청 Neutral Ribonuclease의 분리와 성상에 관한 연구

박성원,이상훈,한중수,고재경 한양대학교 의과대학 1992 한양의대 학술지 Vol.12 No.2

The activity of neutral ribonuclease (RNase) was determined in serum of patients with rhabdomyosarcoma to find out whether the serum enzyme could be used as a marker for rhabdomyosarcoma. Also analysed were isolation patterns of proteins and neutral RNases to investigate the persence of RNase specific to the rhabdomyosarcoma. In serum of patients with rhabdomyosarcoma, activities of amylase, alkaline phosphatase and 5'-nucleotidase were unchanged, but neutral RNase as a marker for the myosarcoma. Neutral RNases in serum of the rhabdomyosarcoma and in its control serum were separated by a DEAE-cellulose column chromatography into 5peaks each and the peak specific to the myosarcoma was not found. The peak I neutral RNase isozyme was, however, observed to be greater in rhabdomyosarcome serum than in the control serum and the peak Ⅴ neutral RNase isozyme was lesser in the myosarcoma than in the control. The peak I neutral RNase isozyme isolated from the rhabdomyosarcoma serum was active toward poly C and less active toward poly U and RNA, indicating the enzyme was the secretory type of RNase in nature. No activity was observed toward purine poly ribonucleotides, polyeosyribonucleotides and double stranded polyribonucleotides. Of the geteropolyribonucleotides used as substrate in the present study, the peak I neutral RNase isozyme from rhabdomyosarcoma serum was highly active toward poly AC and active toward poly ACU, AU, CI, CIU in the decreasing order. No or little activity was observed with poly AG, AGU and ACG. This would indicate that the RNase isozyme is active towasrd linkages made of cytosine and inactive toward linkages with guanine. Studies on the substrate specificity showed differences between the peak I neutral RNase isozymes from rhabdomyosarcoma and control serum, exhibiting decrease in the ratio of poly U/ poly C, RNA/ poly C and heteropolyribonucleotides/ poly C activities in the myosarcoma. This suggerts that the peak I neutral RNase isozyme minght be specific to the rhabdomyosarcoma. DEAE peak I proteins isolated from rhabomyosarcoma and control serum were further separated by a HPLC into 3 peaks each, the protein pattern was similar in DEAE peak I of both serum. The results obtained in the present study indicate that 1) neutral RNaes activity in serum of rhabdomyosarcoma was significantly increased suggesting the possible use of the RNase as a marker ofr the rhadbomyosarcoma and that 2) the peak I neutral RNase as a marker for the rhadbomyosarcoma and that cific to the myosarcome, exhibitin the difference of substrate specifity between the rhabdomyosarcoma and control.

골육종의 억제과정에 미치는 Ribonuclease 의 작용

김성준,이경태,고재경 한양대학교 의과대학 1993 한양의대 학술지 Vol.13 No.1

Neutral ribonuclease (RNase) highly active toward polycyidylate (poly C) was isolated from osteosarcoma tissue by DEAE-cellulose column chromatography and was found to be specific to the bone sarcoma by means of a high performance liquid chromatography (HPLC) and polyacrylamide gel electrophoresis (PAGE). Also studied were substrate specificity and the product analysis of the neutral RNase to understand the role of the enzyme specific to osteosarcoma in pathogenesis of the bone sarcoma. Neutral RNase activity was greatly increased in osteosarcoma tissue, suggesting that in could by used as a marker for the bone sarcoma. Neutral RNases in osteosarcoma tissue were separated by a DEAE-cellulose column chromatography into 5 peaks, of which peaks Ⅵ and Ⅶ RNase isozymes were not found in the control bone tissue. The activity of peak Ⅰ RNase isozyme was most active among the RNase isozymes separated. HPLC and PAGE patterns for the proteins associated with the peak Ⅰ neutral RNase of osteosarcoma appeared to be different those of the control, suggesting that the enzyme might be specific to the bone sarcoma. The peak Ⅰ neutral RNase isozyme specific to the osteosarcoma was found to be active toward ss polyribonucleotide (highly active toward C-C, C-U and A-U linkages). Majority of the poly C digest by the RNase specific to the osteosarcoma was observed to be oligoribonucleotides with chain length of 4-25, indicating that the enzyme was endonuclease in nature. The present study indicate that 1) neutral RNase activity was greatly increased in osteosarcoma tissue, 2) the enzyme could be used as a marker for osteosarcoma, 3) the peak Ⅰ neutral RNase isozyme isolated from osteosarcoma tissue might be specific to the bone sarcoma, 4) the RNase isozyme was active toward ss polyribonucleotide and 5) the RNase isozyme was endofibonuclease in nature, suggesting a possible role of the RNase isozyme in the suppression of the osteosarcoma.

후두암조직의 핵산분해 효소에 관한 연구

태경,김선곤,고재경 한양대학교 의과대학 1995 한양의대 학술지 Vol.15 No.1

Activities of deoxyribonuclease(DNase), ribonuclease(RNase) and RNase inhibitor were determined in the laryngeal cancer tissue to evaluate the use of nuclease and nuclease inhibitor as biochemical markers for laryngeal cancer. Also analysed by a DEAE-cellulose column chromatography were the enzymes and the enzyme inhibitor in the laryngeal cancer tissue to understand the nature of laryngeal cancer in biochemical aspects. Concentration of RNA and protein were unchanged, but DNA content in the laryngeal cancer tissue was significantly in creased. Acid DNase and RNase inhibitor activities were increased, and RNase activity was unchanged in the cancer tissue. The positive rates of DNA content, activities of acid DNase and RNase inhibitor as markers for laryngeal cancer were high, suggesting the use of these three parameters as biochemical markers for the cancer. Proteins in the laryngeal cancer tissue were separated by a DEAE-cellulose column chromatography into 7 peaks, of which a single protein peak was specific to the cancer. Acid DNase was separated as a single enzyme peak and the enzyme thus purified was highly active toward double stranded DNA. Neutral RNase was separated into three isozymes (RNase isozymes Ⅳ, Ⅴ and Ⅵ), of which RNase isozymes Ⅳ and Ⅴ were activated and RNase inhibitor activity associated with these two isozymes were elevated. These results indicated that acid DNase, RNase isozymes Ⅳ and Ⅴ and RNase inhibitors complexed with three isozymes in the laryngeal cancer tissue might play roles in carcinogenesis and suppression of laryngeal cancer.

Consensus building in the resolution of complex environmental issues: A case study of the Hantan River Dam

고재경,김고운,이미홍 한국행정학회 2009 International Review of Public Administration Vol.13 No.3

In this paper, we evaluate a consensus-based solution to a real social and environmental conflict in South Korea. There is increasing concern that the complexity and multi-stakeholder nature of environmental issues demands further consensus-based frameworks to resolve conflicts. However, it is arguable whether the consensus-building approach works in reality. Given the long awaited democracy in South Korean modern history and the debate over the workability of consensus building, it is noteworthy to investigate the first social experiment to adopt deliberative mediation: The parties related to the Hantan River Dam case agreed to use mediation to resolve their conflict. We argue that the institutional scheme should take into account the social systems of which it is a part. Judging the results by the direct outcome criterion, the social experiment to resolve the Hantan River Dam conflict was not successful because the produced agreement was not implemented. This failure has been attributed to the influence of external politics, and representation and accountability problems. There were, however, intangible outcomes such as increased understanding of other parties' interests, resolution of difficult technical issues, and improved relationships.

Effect of Glucocorticoids on Glutamine Metabolism in Rats Treated ith Ammonium Chloride

고재경,김용술 생화학분자생물학회 1984 BMB Reports Vol.10 No.4

泌尿器系 腫瘍組織의 Nuclease 活性度에 대한 硏究

吳達逢,金東翰,高在炅 한양대학교 의과대학 1982 한양의대 학술지 Vol.2 No.1

In order to understand the metabolic significance of changes in the cativities of enzymes involved in degradation of nucleic acids in urologic tumor tissues, the activities of acid DNase, acid RNase and 5'-nucleotidase were determined in tissues of benign prostatic hyperplasia, prostate cancer, bladder cancer and renal cell cancer. Also determined were concentrations of protein, DNA and RNA in these tumor tissues. The activities of the serum enzymes were measured to see whether there were any changes in serum in patients with these tumors. (1) Activities of acid DNase and RNase were greatly increased in tissues of bladder cancer suggesting that these two enzymes may play important roles in carcino-genesis of urinary bladder cells, facilitating the reactions involved in DNA integration and the biosynthesis of cancer specific RNA. (2) In prostate cancer tissues, activities of acid DNase, acid RNase and 5'-nucleotidase were all decreased significantly. The decreases in the enzyme activities may be due, in part, to tissue destruction reported to take place in certain types of cancer and this assumption is supported by the observation that concentrations of protein, DNA and RNA were also decreased significantly in prostate cancer tissues. (3) While the activity of acid RNase was significantly increased in renal cell cancer tissues, activities of acid DNase and 5'-uncleotidase were not changed. (4) In bladder cancer tissues, concentrations of DNA and RNA were significantly increased, but the concentration of protein was unchanged. On the othe hand, concentrations of protein and RNA were significantly increased in renal cell cancer tissues, and the DNA centent was not changed. (5) While activities of serum acid DNase and 5'-nucleotidase were not changed in patients with urologic tumors, the activity of serum acid RNase was significantly increased in patients with prostate cancer. These results suggest that the measurement of serum acid RNase may be an useful tool for diagnosis of prostate cancer.

小兒急性白血病에 있어서 4種의 血淸酵素에 關한 硏究

薛仁俊,朴鍾茂,高在炅 한양대학교 의과대학 1985 한양의대 학술지 Vol.5 No.2

In order to find out whether some of serum enzyme levels could be used as markers for acute leukemia in children, activities of amylase, alkaline phosphatase, 5'-nucleotidase and ribonuclease (RNase) and positive rates of these enzymes as markers for acute leukemia in children were determined in serum of patients with acute lymphocytic leukemia (ALL) and acute non-lymphocytic leukemia (ANLL). Since the activity of neutral RNase in serum was greatly increased in children with acute leukemia, the serum enzyme was isolated and purified using a DEAE-cellulose column chromatography to investigate whether the enzyme specific to acute leukemia in children was present in serum of the patients. The results obtained were as follows; 1. As compared with controls, the activity of serum amylase was significantly increased in patients with ALL, Old. but unchanged in patients with ALL. New. and ANLL. 2. The activity of serum alkaline phosphatase was unchanged in ALL, New. but significantly increased in ALL, Old. and ANLL, New. The positive rates of the enzyme as a marker for acute leukemia in children appeared to be high. 3. The activity of serum 5'-nucleotidase was significantly increased in all ALL. and ANLL. and the positive rates of the enzyme as a marker for acute leukemia in children were relatively high. The activity of this serum enzyme, per se, was considerably low either in controls and the patients with acute leukemia. 4. In all cases of leukemia studied in the present study, the activity of serum neutral RNase and positive rates of the enzyme as a marker for acute leukemia in children were markedly elevated. 5. Serum neutral RNase in patient with acute leukemia in children was isolated in 4 peaks by DEAE-cellulose column chromatography. New peaks not present in control serum was not observed in leukemic serum, but chromatographic pattern of serum RNase in leukemia was not idertical with that in control serum. Of the four serum enzymes studied in the present study, the activity of neutral RNase was markedly increased in leukemic serum and the positive rate of the enzyme as a marker for acute leukemia in children was found to be high. The results suggest that the activity of serum neutral RNase could be used as a marker for acute leukemia in children. It could not, however, be confirmed that the RNase unique to leukemia was present in serum of the children with acute leukemia.

Micropapular Sarcoidosis 1예

고재경,문기찬,최지호,김경진,배지영,서호석,성경제 대한피부과학회 2002 大韓皮膚科學會誌 Vol.40 No.5

Cutaneous sarcoidosis occurs in 20-25% of systemic sarcoidosis and be presented without other organ involvement. Most frequent clicical are maculopapule, nodule, plaque, subcutaneous mokule, infiltrative scar and lupus pernio, but uncommon forms including ulcer, alopecia, hypopiminted and micropapular type have been described. Micropapular type is a rare from of cutaneous sarcoidosis with good prognosis. We present a case of generalized micropapular sarcoidosis without other organ involvement