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金然桂,鄭東孝 中央大學校 食糧資源硏究所 1990 食糧資源硏究所 論文集 Vol.2 No.1
토양 시료로부터 선별과정을 거쳐 생전분을 분해시킬 수 있는 amylase를 생산하는 균주를 분리 하였고, 이 균주(K-1)에 대한 행태적·생리적 성질과 클로닝 가능성 그리고 생전분 가수분해 효소제의 특성을 측정한 결과 분리균주(K-1)는 Bacillus brevis K-1으로 동정되었다. 배양일수에 따른 균의 효소 생산을 측정하였을 때 2∼3일간의 배양에서 최대의 효소활성을 나타내었고 조효소의 최적온도는 35℃, 최적 pH 7.0∼7.5, 온도 안정성은 35℃이하 그리고 pH 안정성은 pH 7.5 이상임을 알 수 있었다. 한편, 효소액의 반응산물에 대한 paper chromatography는 glucose와 maltose가 주 반응 산물인 것으로 보아 본 효소는 당화형 amylase계의 효소임을 알 수 있었다. 그리고 균주의 생육조건은 pH 7.0이고 온도는 35℃로 나타났으며 탄소원에 대한 효소생산은 호화전분 또는 glucose나 maltose를 기질로 하였을 때보다 생전분(CLP)을 기질로 하였을 때 효소 생산력이 2∼5배 이상 높았다. 생전분 가수분해 효소 gene의 HindⅢ 단편과 EcoRV 단편은 pHY300 PLK에 클로닝되었 고 E. coli와 B. subtilis(amy-)에서 발현됨을 알 수 있었다. A bacterium which can utilize raw starch as a sole carbon source was isolated and identified as Bacillus brevis K-1 from its physiological, morphological and cultural properties. Optimal conditions to produce raw starch digesting enzyme were 2∼3 days at pH 7.0 and at 35℃. The crude enzyme had an optimum pH of 7.0∼7.5 and an optimum temperature of 35℃. The enzyme was stable at pH range between 7.0 and 12.0 and at temperature below 40℃. The paper chromatography showed that the crude enzyme was saccharifying amylase. HindⅢ and EcoRV fragments of raw starch digesting amylase gene were cloned in the plasmid pHY300PLK which are E. coli-B. subtilis shuttle vector using E. coli and B. subtilis as a host.
김연계,김유아,신순범,이태식,윤호동 한국식품과학회 2015 Food Science and Biotechnology Vol.24 No.2
The angiotensin-I converting enzyme (ACE) inhibitory activity of Semisulcospira coreana was investigated. Crude CH2Cl2 and MeOH extracts showed 59.3 and 25.6% ACE-inhibitory activities at 5 mg/mL, respectively. After 2 phase separations including n-hexane, 85% aqueous MeOH, n-BuOH, and H2O, the 85% aq. MeOH fraction exhibited the highest ACE-inhibitory activity of 62.1%. The inhibitory activity of 85% aq. EtOH subfraciton 5 (merf5) partially purified using C18 reversed-phase flash chromatography was increased up to 94.6% at 0.5 mg/mL. Typical fatty acids signals observed in 1H NMR and gas chromatography analysis of merf5 and n-BuOH subfractions 6 (burf6) indicated that components of merf5 and burf6 included fatty acids. The fatty acid fractions from S. coreana act as novel ACE inhibitors.
김연계,임치원,연소미,이문희,문호성,조현아,윤나영,윤호동,박희연,이두석 한국수산과학회 2011 Fisheries and Aquatic Sciences Vol.14 No.4
The jellyfish Nemopilema nomurai was hydrolyzed with papain and a novel dipeptide purified via ultrafiltration, gel filtration chromatography with Sephadex LH-20, and reverse phase chromatography using C_(18) and C_(12) columns. The IR, 1H NMR, 13C NMR, and MS spectrometer analyses showed that the dipeptide comprised tyrosine-isoleucine (Tyr-Ile). The IC_(50) and K_i values were 6.56 ± 1.12 and 3.10 ± 0.28 μM, respectively, indicating competitive inhibition of angiotensin-Ι-converting enzyme (ACE). As a novel ACE-inhibitory active peptide, Tyr-Ile may have potential for use in antihypertensive therapy.
김연계,남기호,박선영,김도엽,강상인,한상국,김진수 한국수산과학회 2019 한국수산과학회지 Vol.52 No.1
We investigated the nutritional components of the major commercial frozen seafood products (MCFSP) [sliced frozen- skipjack tuna (ST), -bigeye tuna (BET), -bluefin tuna (BFT), -yellowfin tuna (YT), fish steaks (FST), fish pancakes (FP), fish cutlets (FC), seafood cake balls (SCB), fried shrimp (FS), shrimp patties (SP), shrimp cutlets (SC)] in Korea. All species of sliced frozen tuna and FST were classified as low-calorie foods; the other frozen seafood products were classified as medium-calorie foods. The MCFSP were significant sources of nutritional and functional minerals: the SCB and SC provided calcium; the FST, FC, and SCB provided phosphorus; the BET, YT, and FST provided potassium; the FST, FC, and BFT provided magnesium; the FST, FP and SC provided iron; the SCB, FS, SP, and SC provided zinc; the YT and SCB provided copper; and the FC provided manganese. The total amino acid contents of the MCFSP were in the range of 6.85-26.34 g/100 g. Glutamic acid was the major amino acid in the SCB, FS, SP, and SC. Fatty acid contents were in the range of 386-2,925 mg/100 g; the major fatty acids in the ST, BFT and YT were 16:0, 18:1n-9, 22:6n-3. The MCFSP were not a significant source of vitamin A or riboflavin.
김연계,정은지,이민섭,윤나영,윤호동,김재일,김형락 한국수산과학회 2011 Fisheries and Aquatic Sciences Vol.14 No.4
Inflammatory mediators such as inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) have been implicated in various inflammatory diseases. In this study, we investigated the anti-inflammatory activities of Chondria crassicaulis ethanolic extract (CCE) by measuring its effects on the expression of iNOS and COX-2 proteins in lipopolysaccharide (LPS)-treated RAW 264.7 murine macrophages. CCE significantly and dose-dependently inhibited the LPS-induced release of nitric oxide and prostaglandin E_2, and suppressed the expression of iNOS and COX-2 proteins in LPS-stimulated RAW 264.7 cells, without causing any cytotoxicity. It also inhibited the production of the pro-inflammatory cytokines such as interleukin (IL)-1β, IL-6, and tumor necrosis factor (TNF)-α in LPS-stimulated RAW 264.7 cells. Moreover, treatment with CCE strongly suppressed nuclear factor-κB (NF-κB) promoter-driven expression in LPS-treated RAW 264.7 cells. CCE treatment blocked nuclear translocation of the p65 subunit of NF-κB by preventing proteolytic degradation of inhibitor of κB-α. These results indicate that CCE regulates iNOS and COX-2 expression through NF-κB-dependent transcriptional control, and identifies potential candidates for the treatment or prevention of inflammatory diseases.
박기의,김연계,임치원,서영완,장미순,박희연 한국응용생명화학회 2005 Journal of Applied Biological Chemistry (J. Appl. Vol.48 No.4
Antioxidant activity of the ethanol extract from boiled-water of Hizikia fusiformis (EBH) were compared with those of BHA, L-ascorbic acid, gallic acid, caffeic acid and (-)-catechin. The free radical scavenging ability against DPPH (1,1-diphenyl-2-picrylhydrazyl), authentic peroxynitrite and reducing power were measured as indices of antioxidant activity. EBH showed the potent DPPH radical and peroxynitrite scavenging activities, showing 85.23 and 96.97% at final concentration of 1000 µg/ml, respectively. The reducing power increased with the increasing amount of EBH (final concentration of 1, 10, 100 and 1000 µg/ml). Total phenolic content of EBH was 588 mg (-)-catechin/g at the final concentration 1000 µg/ml. Total phenolic contents correlated with DPPH radical scavenging activity (R2= 0.766) and reducing power (R2= 0.944). These results suggested that EBH could be a natural antioxidative source containing antioxidative components.
이문희,김연계,문호성,김경덕,김관규,조현아,윤나영,심길보,박희연,이동석,임치원,윤호동,한상국 한국식품과학회 2012 Food Science and Biotechnology Vol.21 No.5
The proximate composition, fatty acid, and amino acid profile of the body wall and viscera of each red and black sea cucumber (Apostichopus japonicus) from Ulleungdo(Island) and Dokdo(Island) in Korea were compared. Moisture, ash, crude protein, and crude lipid contents ranged between 80.26-91.49, 2.57-6.85, 1.13-3.99, and 0.14-2.12%, respectively. The fatty acid values varied depending on the species and the regions of collection. The anteiso C17:0, C16:1Δ9, C17:1Δ7, C18:1Δ11, and C16:2Δ7 were only observed in the body wall. Among the tested fatty acids, the C18:1Δ11 was specific in red sea cucumber, and C20:4Δ6 (17.7%) and C20:5Δ3 (17.6%)were the predominant polyunsaturated fatty acids (PUFA)in all samples. The contents of the C18:0 dimethyl acetal (C18:0 DMA), C16:1Δ7, C16:1Δ5, and C18:1Δ5 were compared in details. Total amino acids (TAA) of body wall were 1.3-1.9 times higher than those of viscera. Glutamic acid and aspartic acid constituted the major TAA of sea cucumbers. The ratio of essential amino acids (EAA):nonessential amino acids (NEAA) on TAA ranged from 1.15 to 0.67 of sea cucumbers. Viscera of red sea cucumber from Dokdo(Island) were rich in free amino acids (FAA)and showed a high content in leucine.
이문희,김연계,윤나영,심길보,Natalya M. Aminina,Irina A. Kadnikova,임치원 한국수산과학회 2012 Fisheries and Aquatic Sciences Vol.15 No.4
We investigated ultraviolet (UV) absorption materials from Russian seaweeds. First, the UV absorptivities of five seaweeds Gloiopeltis fucatas, Mazzaella sp., Mastocarpus pacificus, Laminaria cichorioides, Saccharina japonica were evaluated by a UV spectrometer. Of these seaweeds, Gloiopeltis fucatas and Mazzaella sp. showed high levels of UV absorption. Column chromatography of active 50% aqueous ethanol extracts from Gloiopeltis fucatas and Mazzaella sp. resulted in the purification of two known compounds. Spectroscopic techniques identified their structures as shinorine and palythine. These materials exhibited UV absorptive capabilities at wavelengths of 333 and 320 nm, respectively. These results suggest that Gloiopeltis fucatas and Mazzaella sp. may be useful as natural cosmeceutical sources.