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Botrytis cinerea에 의한 바이올렛 잿빛곰팡이병
김형무 한국식물병리학회 2011 식물병연구 Vol.17 No.1
Gray mold caused by Botrytis cinerea occurred on Saintpaulia ionantha in flower shop of the Jeonju city in Korea. Typical symptoms with brown water-soaked and rotting lesions were appeared on the flowers, leaves and petiole of infected plants. Many conidia spores appeared on the lesions under humid conditions. Colonies were grayish brown and sclerotial formation on potato dextrose agar. Conidia were one celled, mostly ellipsoidal or ovoid in shape, and were colorless to pale brown in color. The conidia were 7~14×5~9 μm in size. Based on pathogenicity and morphological characteristics of the isolated fungus, the causal fungus was identified as B. cinerea Persoon: Fries. Gray mold of S. ionantha was proposed to the name of this disease. 전북 전주시 화원에서 Botrytis cinerea에 의한 바이올렛 잿빛곰팡이병이 발생하였다. 병징은 꽃, 잎과 잎자루가 수침상으로 물러지고 부패되었다. 다습조건에서 병반위에 잿빛색의 분생포자가 많이 형성하였다. 균총은 PDA 배지에서 회갈색이며 균핵을 형성하였다. 분생포자는 단포자, 난형 또는 타원형으로 무색 또는 옅은 갈색이고, 크기는 7~14×5~9 μm이었다. 바이올렛에 발생한 병징과 병원균의 균학적 특징을 조사한 결과 B. cinerea Persoon:Fries에 의한 바이올렛 잿빛곰팡이병으로 명명할 것을 제안한다.
무우 立枯性 羅病株에서 分離된 Rhizoctonia solani Kuhn의 菌사融合群에 관한 硏究
金炯武,鄭性洙,蘇仁永 전북대학교 농업과학기술연구소 1984 農大論文集 Vol.15 No.-
The experiments were conducted on the radish growing fields in Jeonbug province, 1983. Anastomosis phenomena among 280 isolates of Rhizoctonia solani that isolated from radish plants were divided into five groups and seven types (AG1 IA, AG1 IB, AG2-1, AG2-2, AG3, AG4, AG5). Each anastomosis groups distribution was AG1 IB(40%), AG3(25.7%), AG2-1 (12.5%), AG 4(12.5%), AG2-2(3.6%), AG 5 (3.2%), AC 1 IA (2.5%). AG 1 IB (64.5%) in Wanju. AG 3 (43.2%) in Jinan, AG 3 (37.0錄) in Jeongeub distributes highly. A number of nuclei Per a hyphal cell in each anastomosis groups were polynuclei (3-13), AG5 was very much (7.8), AG4 was fewer (5.5). Hyphal growth rapidity of each anastomosis groups was difference, and AG1 IA type grew rapidly (30.0mm/24hrs.), AG2-1 was slightly (17.0mm/24hrs.). Main hyphal width of each anastomosis groups was 6-11.5㎛, AG3 was widest(10.1㎛) and AG4 was narrowest (7.6㎛).
수박 종자에서 Pseudomonas pseudoalcaligenes subsp. citrulli의 분리를 위한 선택배지
金炯武,李光植 全北大學校 1993 論文集 Vol.36 No.-
A semiselctive agar medium(PSC) was developed for the isolation of Pseudomonas pseudoalcaligenes subsp. citrulli(Ps.c.) from watermelon seed. The medium contains following per liter : 20g proteose peptone #3, 5.0㎖ glycerol, 1.5g K2HPO4, 1.5g MgSO47H2O, 15g agar, 1㎎ ampicillin, 100㎎ cycloheximide 1㎖ 1.5% bromthymol blue, 1㎖ 1.0% methyl violet and 1㎎ vancomycin. Colonies of Ps.c. were 3-4㎜ in diameter, smooth, round, slight convext, dark green(lighter margin) and glistening after 5 days at 39C on the PSC medium. The recovery of 10 strains including ATTC type 29625, on PSC medium in comparison to KB ranged from 93% to 105%(mean 97.8%). Reduction in seed flora of 10 watermelon seed lots on PSC medium was 97.2%. Recovery of Ps.c. added to watermelon seed ranged from 91.3% to 103.4%(mean 96.7%).
감나무 가지마름 (枝枯) 병 症狀에 關與하는 病原菌의 分離
金炯武,李貴宰,蘇仁永,金又喆,崔貞植 전북대학교 농업과학기술연구소 1992 農大論文集 Vol.23 No.1
A black spot and dieback symptom did occur on persimmon trees in Chonbuk plovince in August 1991. Isolated fungi from stems and twigs were Gloeosporium sp., Alternaria sp., Fusarium sp., Penicillium sp., and not-identification fungi, The pathogenicity of Isolated fungi were as follows : the leaves appeared with the black spot symptom by genus of Pestalotia species, Gloeosporium species, Alternaria species and four not-identification fungi, and the stems appeared with the exetreme dieback symptom by genus of Pestalotia sp., Gloeosporium sp. and two not identification fungi.
硅酸質系微分末混合 塗布防水材料가 시멘트모르터의 透水性 및 細孔量에 미치는 影響
김형무 서울産業大學校 1994 논문집 Vol.39 No.1
This paper describes the effect of siliceous coating on water permeability and pore volume of mortar substrate. Coefficient of permeability and pore volume were measured for evaluation of watertightness of the.. Coefficient of permeability and pore volume of coated mortar were obviously less than those of non-coated one. Effect of siliceous coating reached the inside 5 to 10mm of coated morta. Reduction of pore volume was caused by the decrease of capillary pores within the size range 0.1 to 1/μm. Relationship of them can be described by the equation y=3.25×10?x??, where y is coefficient of permeability and x is pore volume.
고추냉이 세균성 근경 부패병의 발생 및 병원균의 분리 동정
김형무,송완엽,강미형,소인영 전북대학교 농업과학기술연구소 1996 農大論文集 Vol.27 No.1
A disease occurrence of rhizome rot at the Japanese horse radish growing fields in Chonbuk area was investigated and soft rot-causing bacteria were isolated and identified by morphological and biochemical characteristics to verify a causal bacterium of rhizome soft-rot disease of Japanese horse radish. The rhizome soft-rot was occurred on April and continued to October·. It was most severe on August but decreased on September. Disease occurrence reflected regional difference such as 38% in Chonju and Iksan and 27% in Muju. Four major bacterial genera, Erwinia, Pseudomonas, Xanthomonas and Corynebacterium, were isolated and showed the isolation frequency of 54.6%, 17.2%, 21.2% and 6.8%, respectively. They also showed a seasonal fluctuation of isolation frequencies in genera and Erwinia sp. was the most dominant species during the high temperature season. Rhizome soft rot-causing isolates were selected by the pathogenicity test. They showed negative results in Gram's staining, growth in 5% NaCl and growth on KCN media but positive in casein hydrolysis and gelatin liquefaction. They utilized galactose, lactose, trehalose and citrate but did not utilize maltose, melibiose, raffinose and inullin. They also showed the soft-rot symptom on the petiole and rhizome of the horse radish but weak soft rot on potato tuber. This causal pathogen was identified as Erwinia carotovora subsp. wasabiae by these results of bacteriological characteristics.