Susceptance를 이용한 피부수화도 측정 장비의 개발 및 평가

김홍식,장우영,신건수,조백환,김인영,김선일,Kim, Hong-Sig,Jang, Woo-Young,Shin, Kun-Soo,Cho, Baek-Hwan,Kim, In-Young,Kim, Sun-I. 대한의용생체공학회 2008 의공학회지 Vol.29 No.6

In this paper, a novel system is proposed to measure skin hydration using the susceptance method. This system largely consists of a low-voltage(${\pm}2.6$ V) driven circuit and minimized electrodes of size($5{\times}5mm^2$). To evaluate the accuracy of the novel system in measuring skin hydration, skin hydration values from 105 subjects are measured by the proposed system. The measurements are then compared to those obtained by the golden reference device based on the capacitance method in terms of Intraclass Correlation Coefficient(ICC) and correlation coefficient. All measurements are performed on 7 sites, which are forehead, Crow's foot, cheek, chin, volar forearm, dorsal forearm, and back of the hand, in a room where the temperature and humidity are maintained at an uniform level of $22{\pm}2^{\circ}C$ and $50{\pm}5%$, respectively. ICC values are above 0.9(p=0.001), signifying that the skin hydration values measured by the two methods show a good level of reliability. Correlation coefficient between the two methods is also 0.562(p=0.001). Based on these results, it is expected that the proposed system may be applicable in a variety of clinical or cosmetic areas.

有 · 無限伸育型 콩 品種間 雜種初期世代에서 量的形質들에 대한 組合能力

Hong Sig Kim(金弘植),Eun Hi Hong(洪殷熹),Seok Dong Kim(金奭東),Sang Il Park(朴相一),Seung Keun Jong(鄭丞根) 한국육종학회 1994 한국육종학회지 Vol.26 No.3

This study was conducted to study on the inheritance of growth characters and yield components at the Crop Experiment Station, Rural Development Administration, Suwon, Korea in 1990. Some quantitative characters in F₁ and F₂ of crosses between four determinate soybean cultivars as male parents(Hwangkeumkong, Jangyeobkong, Paldalkong and Suwon 148) and two indeterminate soybean cultivars as female parents(Clark and L63-2404) were investigated and analyzed for combining ability. In F₁ general combining ability(GCA) of male parents indicating principal contribution of additive genetic effects was significant in stem height, number of nodes on mainstem, number of branches, number of nodes per plant, and mainstem dependence rate of number of nodes. GCA of male and female as well as specific combining ability(SCA) was significant in days to maturity, suggesting that additive and non-additive genetic effects of male and female act simultaneously. Both GCA and SCA effects were not significant in yield components. In F₂, both GCA of male and SCA effects were significant in stem height, number of nodes, number of pods, mainstem dependence rate, and grain weight per plant. GCA effect of male was significant in number of branhces. GCA effects of both male and female were significant in days to maturity and 100 seed weight. Non-additive genetic effects were shown in number of pods on mainstem, number of pods per plant and number of grains per plant. The significance and directions of GCA and SCA effects varied according to males and females, generations and relevant characters. According to the estimated GCA, Clark and Deogyukong were found to be desirable parents for greater vegetative growth and yield, whereas Suwon 148 for earlyness, Hwangkeumkong for large seed size, and Paldalkong for high mainstem dependence rate and short stem. The estimated SCA effect for yield and lateness was great in the crosses of L63-2404×Dogyukong and Clark×Suwon 148, while SCA effect for earlyness was large in L63-2404×Suwon 148 and Clark×Dogyukong.

有ㆍ無限伸育型 콩 品種間 雜種初期世代에서 量的形質의 遺傳力 및 形質間 相互關係

Hong Sig Kim(金弘植),Sang Il Par(朴相一),Seung Keun Jong(鄭丞根),Eun Hi Hong(洪殷熹),Seok Dong Kim(金奭東) 한국육종학회 1994 한국육종학회지 Vol.26 No.4

This study was carried out at the Crop Experiment Station, Rural Development Administration, Suwon, Korea in 1990. F₁ and F₂ generations from crosses between four determinate soybean cultivars of male parents(Hwangkeumkong, Jangyeobkong, Paldalkong and Suwon 148) and two indeterminate soybean cultivars of female parents(Clark and L63-2404) were used as materials to study on the inheritance of growth characters and yield components. Heritability estimate for days to maturity was fairly high. Stem height, number of nodes on mainstem and mainstem dependence rate of number of nodes and pods had relatively high heritabilities. The heritability of branch-related and yield-related characters was low. Genotypic and phenotypic correlations were significantly positive between seed weight per plant and most of growth or yield components except 100-seed weight. But highly negative correlations were existed between seed weight per plant and mainstem dependence rate of number of nodes and pods. Path corefficient analysis revealed that number of seeds per plant had the greatest direct effect on seed weight per plant, while the total number of nodes per plant and number of nodes on branches showed the greatest direct effects on the number of seeds poer plant.

주제발표 : 제2주제 ; 벤처기업 활성화를 위한 대학창업보육센터의 역할 - 주성대학 주성테크노창업보육센터를 중심으로 -

김홍식 ( Kim Hong Sig ) 충북개발연구원 1999 벤처기업 육성방안과 전략 Vol.- No.-

저장방법이 삼백초 생근경 품질에 미치는 영향

김인재(Kim, In-Jae),김민자(Kim, Min-Ja),남상영(Nam, Sang-Young),윤태(Yun, Tae),김홍식(Kim, Hong-Sig),홍성수(Hong, Seong-Su),황방연(Hwang, Bang-Yeon) 한국자원식물학회 2005 한국자원식물학회 학술심포지엄 Vol.2005 No.-

기계수확용 다수성 조생 수수 ‘청풍’

윤건식(Geon-Sig Yun),이재웅(Jae-Wung Lee),황세구(Se-Gu Hwang),김익제(Ik-Jei Kim),홍성택(Seong-Taeg Hong),최명은(Myeong-Eun Choe),최규환(Gyu-Hwan Choi),김용순(Yong-Soon Kim),김홍식(Hong-Sig Kim) 한국육종학회 2019 한국육종학회지 Vol.51 No.4

To improve their income, farmers should cultivate sorghum varieties with high productivity, short breeding period, and easy double cropping. To increase purchases by consumers, the sorghum variety must have health-related functions. To meet these demands, the Cheongpung variety has been raised through net separation from the traditional species of the Chungbuk region as a sorghum variety that can be harvested by machine, have the highest yield in the country, and have the most abundant antioxidant content. The seed coat of ‘Cheongpung’ was reddish-brown, and had the characteristic of early maturation; it required only 107 days from sowing to harvest. Its culm length was 89 cm, the smallest among domestic cultivated varieties. It could also be harvested by machine without difficulty. Compared with the ‘Hwanggeumchal’ variety, the number of ear per plant in ‘Cheongpung’ was approximately 1.3, but each ear piece weighed 66.2 g, weighing 3.8 g, and the thousand-grain weight was 1.4 grams higher than the rough-grain weight. A local adaptation test in five regions nationwide for two years led to a ‘Cheongpung’ yield of 359 kg/10a, which was the highest among domestic cultivated varieties. In addition, the polyphenol content of ‘Cheongpung’ was 29% higher than that of ‘Hwanggeumchal’. (Registration No. 7717)

프로테옴 해석에 의한 벼 게놈 기능해석과 응용

우선희,김홍식,송범헌,이철원,박영목,정승근,조용구,Woo, Sun-Hee,Kim, Hong-Sig,Song, Berm-Heun,Lee, Chul-Won,Park, Young-Mok,Jong, Seung-Keun,Cho, Yong-Gu 한국식물생명공학회 2003 식물생명공학회지 Vol.30 No.3

In this review, we described the catalogues of the rice proteome which were constructed in our program, and functional characterization of some of these proteins was discussed. Mass-spectrometry is the most prevalent technique to rapidly identify a large number of proteome analysis. However, the conventional Western blotting/sequencing technique has been used in many laboratories. As a first step to efficiently construct protein cata-file in proteome analysis of major cereals, we have analyzed the N-terminal sequences of 100 rice embryo proteins and 70 wheat spike proteins separated by two-dimensional electrophoresis. Edman degradation revealed the N-terminal peptide sequences of only 31 rice proteins and 47 wheat proteins, suggesting that the rest of separated protein sports are N-terminally blocked. To efficiently determine the internal sequence of blocked proteins, we have developed a modified Cleveland peptide mapping method. Using this above method, the internal sequences of all blocked rice proteins(i, e., 69 proteins) were determined. Among these 100 rice proteins, thirty were proteins for which homologous sequence in the rice genome database could be identified. However, the rest of the proteins lacked homologous proteins. This appears to be consistent with the fact that about 45% of total rice cDNA have been deposited in the EMBL database. Also, the major proteins involved in the growth and development of rice can be identified using the proteome approach. Some of these proteins, including a calcium-binding protein that tuned out to be calreticulin, gibberellin-binding protein, which is ribulose-1.5-bisphosphate carboxylase/oxygense active in rice, and leginsulin-binding protein in soybean have functions in the signal transduction pathway. Proteomics is well suited not only to determine interaction between pairs of proteins, but also to identify multisubunit complexes. Currently, a protein-protein interaction database for plant proteins(http://genome.c.kanazawa-u.ac.jp/Y2H)could be a very useful tool for the plant research community. Also, the information thus obtained from the plant proteome would be helpful in predicting the function of the unknown proteins and would be useful be in the plant molecular breeding.

피복재료가 층층갈고리둥글레의 생육, 收量 및 잡초발생에 미치는 影響

김인재(Kim In Jae),김민자(Min Ja Kim),남상영(Sang Young Nam),이철희(Cheol Hee Lee),김홍식(Hong Sig Kim) 韓國雜草學會 2003 Weed&Turfgrass Science Vol.23 No.1

나물콩 재배시기가 종실 품질에 미치는 영향

김학신,김홍식,김경호,Kim, Hag-Sin,Kim, Hong-Sig,Kim, Kyong-Ho 한국작물학회 2006 Korean journal of crop science Vol.51 No.suppl1

These studies were conducted to clarify the effects of sowing date on seed quality characters in soybean-sprouts. Six sprout-soybean cultivars were planted on three sowing dates in 2000 and 2001. Percentages of purple spot seed stain, seed coat-cracking and immatured seeds were higher in the May 25 sowing than later sewings. Pungsannamulkong showed the lowest percentages of seed coat-cracking and immatured seeds, while Hannamkong showed the highest percentages. The protein contents decreased as the sowing dates were delayed. Tawonkong and Pungsannamulkong showed the highest protein content on May 21 and June 15 sewings, and on July 5 sowing, respectively. The oil contents was not affected by the sowing dates, however, Somyeongkong and Tawonkong showed the highest and the lowest oil contents, respectively. The aglycone contents of isoflavone were the highest in the lune 15 sowing followed by July S sowing and May 25 sowing in that order. Somyeongkong showed the highest contents of isoflavone and aglycone, while Tawonkong showed the lowest contents. 본 연구는 나물용 콩의 파종기가 콩나물 원료콩의 품질에 미치는 영향을 구명함으로써 양질 콩나물 품종육성과 재배기술 확립을 위한 기초 자료를 얻고자 $2000{\sim}2001$년에 호남농업연구소 전작시험포장에서 파종시기 시험과 콩나물 간이검정실에서 콩나물생장 시험을 수행하였다. 시험 결과를 요약하면 다음과 같다. 1. 종실의 자반립, 열피립 및 미숙립 발생율은 5월 25일 파종이 6월 15일과 7월 5일 파종에 비하여 높았고, 풍산나물콩이 자반립, 열피립 및 미숙립 발생율이 가장 낮았으며 한남콩이 발생율이 가장 높았다. 2. 조단백질함량은 파종기가 늦어지면서 감소하였고, 5월 25일과 6월 15일 파종에서 다원콩이 가장 높았고 7월 5일 파종에서는 풍산나물콩이 가장 높았다. 조지방함량은 파종기에 따른 일정한 경향이 없었으며 소명콩이 가장 높았고 다원콩이 가장 낮았다. 3. Isoflavone의 aglycone 함량은 6월 15일 파종이 가장 많았고 다음으로 7월 5일파종이 많았으며, 5월 25일파종이 적었고, 품종간에는 소명콩이 가장 많았고 다원콩이 가장 낮았다. 4. 종실의 품질향상을 위한 나물콩의 파종기는 지나친 일찍 또는 늦게 심는 것 보다 품종 특성을 고려하여야 할 것으로 판단되었다.

중부지방에서의 파종시기가 들깨의 생육 및 수량에 미치는 영향

김인재(Kim In Jae),김민자(Min Ja Kim),남상영(Sang Young Nam),이철희(Cheol Hee Lee),김홍식(Hong Sig Kim) 한국자원식물학회 2002 한국자원식물학회지 Vol.15 No.1

중부지방에 있어 들깨 파종한계기를 구명하기 위하여 대엽들깨를 시험품종으로 하여 4월 30일부터 7월 20일까지 20일 간격으로 파종을 실시하여 얻은 결과를 요약하면 다음과 같다. 1. 만파할수록 출아는 단축되었으며, 개화기, 성숙기는 늦어졌나 개화 후 성숙기까지의 소요일수는 차이가 미미하였다. 2. 파종기가 늦어짐에 따라 경장, 경태, 주당 분지수 그리고 주경절수는 감소하는 경향이었다. 3. 만파할수록 화방군장은 길었으나 화방군수, 화방군당 삭수는 적어지는 경향이었고, 1,000립중은 무거웠다. 4. 종실수량은 6월 10일까지의 파종에서는 차이가 없었으나, 6월 30일 파종에서는 10% 정도 감수되었으며, 7월 20일 파종에서는 62% 수준으로 감수 폭이 컸다. To investigate the critical seeding date for Daeyeupdlkkae in the middle area of Korea, we seeded from 30 April to 20 July with 20-day intervals on the experimental field in Chungbuk-Do agricultural research and extension service. As seeding date was delayed, days to emergence were shortened, flowering and maturing date were delayed, but maturing period was not significantly affected by seeding date. Delayed seed date resulted in decreased stem length, culm diameter, number of branches per plant and number of nodes on the main stem. Cluster length and 1,000 grain weight increased but number of cluster per plant and number of seed capsules per cluster decreased with delayed seeding. Seeding date up to 10 June showed no effected on grain yield, but 30 June and 20 July decreased 10% and 38%, respectively.