고양이 감각 및 운동겉질에서 calbindin D-28k와 parvalbumin에 대한 면역세포화학적연구

김종중,장인엽,정윤영,윤상필,문정석 대한해부학회 1998 Anatomy & Cell Biology Vol.31 No.2

고지방 식이 및 참당귀의 엑기스 투여가 흰쥐 관상동맥벽의 탄력섬유에 미치는 형태학적 연구

김종중,문정석,정주현,문영희 朝鮮大學校 附設 醫學硏究所 1983 The Medical Journal of Chosun University Vol.8 No.1

Observation of the elastic fibers of the coronary artery walls in adults rats has been carried out by administering a high fat diet for 100 days and Angelica gigas Nakai extract for 20 days. The results obtained are summarized as follows : 1. The development of the elastic fibers in the coronary artery walls was much poorer in the high fat diet group than in the standard diet group. 2. The development of the internal elastic membrane in the tunica intima was rich in all experimental groups. 3. Scattered irregular fibers and fine and cut elastic fibers were observe in the media of all groups. 4. External elastic membranes and adventitia were not observed in any of the groups. 5. Highest development of internal elastic membranes was observed in the A, B-IV and C-IV, groups which were standard diet and Angelica gigas Nakai extract administration groups. 6. Poor development of internal elastic membranes was observed in groups B-III and C-II. 7. There was high development of the internal elastic membranes in groups fed the Angelica gigas Nakai extract.

모체 Thyroxine 투여가 태아알코올효과를 가진 흰쥐 대뇌겉질 및 해마에서 NPY함유 신경세포의 생후 발달에 미치는 영향

김복,박상기,박영란,김종중,문정석,김주수,문영민,현영식,천관영,정윤영 朝鮮大學校 附設 醫學硏究所 2005 The Medical Journal of Chosun University Vol.30 No.2

Background and Objectives: Maternal alcohol abuse is considered to be one of the most prominent cause of neurobiological malformations in the postnatal and adult life of the offspring. In this study, we investigated the effects of maternal alcohol drinking on the postnatal development of NPY-containing neuron, and, the influence of thyroxine treatment on the cerebral cortex and hippocampus of pups of alcohol abused mother. Materials and Method: Time-pregnant rats were divided into three groups. Alcohol-fed group A received 35 calories of liquid alcohol diet daily from gestation day 6; control pair-fed group B was fed a liquid diet in dextrin replaced alcohol isocalorically: alcohol + T4 group C received 35 calories liquid alcohol diet and exogenous thyroxine subcutaneously. Results: Group C showed prominent NPY immunoreactivity in the cerebral cortex compared to group A and B at P7. In group C, NPY-containing neurons were widely distributed in the all layers of cerebral cortex after P14. Besides, numerical decrease of NPY-containing neuron as age increases was not found in group C. However, the decrease of NPY-containing neuron was clearly observed in group A compared to group C after P14. In hippocampus, group Band C were appeared similar patterns after P7. Additionally, in group C, NPY immunoreactivity was prominently appeared in CA2 and CA3 at P14 as compared to group B. Conclusions: The present results showed the increase of intensity and number of NPY-containing neurons in cerebral cortex and hippocampus of pups of exogenous T₄ supplemented alcohol-exposed dams as compared to control pair-fed and alcohol-exposed pups at P7. It presumably suggest that NPY-containing neurons might be regulated by the early postnatal growth stimulatory effect of the exogenously supplemented T₄. Therefore, the increase of NPY synthesis caused by maternal administration of exogenous thyroxine may ameliorate fetal alcohol effect, one of the ill effects as a result of the dysthyroid state following maternal alcohol abuse.

척수를 손상시킨 후 꼬리정맥에 주입한 사람탯줄혈액세포가 뇌줄기에 미치는 영향

김종중,정윤영,박영란,문영민,현영식,정영욱,문정석 朝鮮大學校 附設 醫學硏究所 2007 The Medical Journal of Chosun University Vol.32 No.1

Background and Objectives: Stem cells are a valuable resource for treatment of many disease, but limited access to stem cells in some organs such as brain restricts their utility. Many approaches have been attempted to restore the function following brain stem injury (BSI) and spinal cord injury (SCI). The use of the human umbilical cord blood cells (hUCB) - a rich source of nonembryonic or adult stem cells - has recently been reported to ameliorate the behavioral consequences of stroke. Mateiials and Methods: Forty rats were divided into 8 groups: (1) SCI l+hUCB (infused 1 day post injury); (2) SCI 2+hUCB (infused 2 days post injury); (3) SCI 3+hUCB (infused 3 days post injury); (4) SCI 4+hUCB (infused 4 days post injury); (5) SCI 5+hUCB (mfusedt 5 days post injury); (6) SCI 6+hUCB (infused 6 days post injury); (7) LO+hUCB (laminectomy+hUCB); and (8) LO (laminectomy only). SCI was produced by compressing the spinal cord for one minute with an aneurysm clip calibrated to a closing pressure of 50 g. We report here that immunhistotochemical identification of fluorescent hUCB positive cells in the brain stem after compressed spinal cord injury using mouse anti-human mitochondria monoclonal antibody (MAB1273). Results: All SCI+hUCB (1-8) groups contained fluorescent hUCB positive cells in the all area of the brain stem. But especially a large number of fluorescent hUCB positive cells were observed in the whole area of the brain stem of the experimental 5 (SCI 5+hUCB) and 6 (SCI 6+hUCB)groups. No hUCB positive cells were found in the brain stem of group with non-injured spinal cord of these animals and group with laminectomy only. Conclusion: These results suggest that hUCB are potentially useful as a vector for treating a variety of the central nervous system disorders, and we are sure that continuous of stem cell study will give an best opportunity to treat the uncurable disorders in the future.

園藝栽培 團地에서의 진딧물相에 關한 硏究

金宗中 조선대학교 농업연구소 1980 農業硏究 Vol.1980 No.1

園藝作物 裁培地域에서의 진딧물相을 調査하기 爲하여 진딧물 採集器(Yellow pan Trap)에 依해 1979年 5月1日부터 同年 10月 末日까지 調査한 바 다음과 같은 結果를 얻었다. (1) 本 調査 地域에서는 植物 Virus病을 媒介하는 28種의 진딧물을 포함여 46種이 分布되어 있으며 Virus 媒介 진딧물의 數는 全 採集 個體水의 88.32%였다. (2) 本 調査 地域 진딧물 個體數의 78.69%를 占하는 優占種 8種은 다음과 같다. (1) 복숭아 흑 진딧물(Myzus persicae(sulzer) (2) 양배추 가루 진딧물(Brevicoryne brassicae (Linnaeus) (3) 목화 진딧물(Aphis gossypii Glover) (4) 아카시아 진딧물(Aphis craccivora koch) (5) 무우 테두리 진딧물(Lipaphis erysimi Kaltenbach) (6) 조팝나무 진딧물(Aphis spiracola patch) (7) 소루쟁이 진딧물(Aphis rumicis Linnaeus) (8) 쟈바 못털 진딧물(Capitophorus Lippophaes javanieus H.R Lambers) (3) 季節的 發生型은 溫帶型인 春, 秋2回의 Peak를 이루고 있으며 이 時期에 發生하는 진딧물數는 全個體數의 78.69%였다. In order to investigate the aspect of Aphis on the growing areas of garden plants, investigation was done from May 1st, 1979 till the end of October of the same year. The following results were. obtained: 1. 46 kinds of Aphids including 28kinds carrying the palnt Virus Inffection were distributed on the investigated areas, the number of Aphids carrying. Virus Inffectioh was 88.32% of the whole sample. 2. In these investigated areas, the 8 predominant species occupying 78.69% of the individual number of Aphids were as follows: (1) Myzus persicae (Sulzer) (2) Brevicoryne brassicae (Linnaeus) (3) Aphis gossypii Glover (4) Aphis craccivora Koch (5) Lipaphis enysimi kaltenbach (6) Aphis spiraecola Patch (7) Aphis rumicis Linnaeus (8) Capitophorus hippophaes javanicus H. R. Lmabers 3. The temperate zone type of seasonal development, Spring and Autumn, was the most dominant, the number of Aphids developing in this period was 78.69% of the whole sample.

韓國人 成人 男子에 나타난 非 正常的인 脾臟의 1例

김종중,문정석,정주현 朝鮮大學校 醫學硏究所 1980 The Medical Journal of Chosun University Vol.- No.-

Authors took an abnormal spleen from a korean male adult cadava aged about 52 years in the Anatomy department of medical College, Chosun University. After dissection, we report views on autopsy and discussion on references. Particular matters were as follows 1. Spleen weight : 3,760gm ; Speen length : 32,50cm ; Spleen width : 17, 25cm, Spleen thickness : 11,50cm. 2. Spleen type : tetrahedral type.

손상된 척수에 이식한 사람배꼽혈액이 척수 기능회복에 미치는 영향에 관한 연구

김종중(Jong-Joong Kim),정윤영(Yoon-Young Chung) 대한해부학회 2003 Anatomy & Cell Biology Vol.36 No.3

최근 각광받고 있는 성체줄기세포로 잘 알려진 사람배꼽혈액세포(hUCB)는 태아를 출산할 때 산모의 배꼽이나 태반에 서 쉽게 얻을 수 있으며 뇌졸중을 포함한 신경성퇴행질환과 혈우병, 팡코니증후군 각종 암, 면역계통의 질병을 치료할 수 있는 자원이 풍부한 세포로서 동물은 물론 사람에게도 직접 이용하여 좋은 성과를 얻고 있다. 본 실험에서는 척수를 인위 적으로 손상 (SCI)시킨 후 hUCB세포를 꼬리정맥을 통하여 주입하여 척수기능의 회복상태를 확인하고자 본 실험을 시도 하였다. 흰쥐 25마리를 5개의 실험 군 (LO, LO±hUCB, SCI 1±hUCB, SCI 5±hUCB, SCI)으로 나누었다. SCI는 55 g 정도 압력을 유지할 수 있는 특수하게 제작한 정맥 집게 (clip)를 이용하여 척수를 압박 손상시켰다. hUCB세포를 주입시킨 후 1, 2, 3주 간격으로 맨눈관찰 (open field), 철망경사오르기 (grid incline), 뒷다리펴기 (leg extention), 발가락펴기 (toe spread)를 실시하였다. hUCB양성반응세포를 확인하기 위하여 면역형광염색과 면역조직화학염색을 실시하였다. SCI 실험 군에서 hUCB세포가 신경아교세포와 신경세포로 분화하는 양상을 관찰할 수 있었으나 LO와 LO±hUCB 실험 군에서는 관찰할 수 없었다. 행동평가에서 맨눈관찰, 철망경사오르기, 뒷다리펴기, 발가락펴기는 SCI 5±hUCB 실험 군이 다른 실험 군에 비 하여 척수 기능이 매우 향상되었으며 SCI 실험 군에서는 3주가 지나도 행동은 향상되지 않았다. 이는 척수를 손상시킨 후 곧 바로 hUCB세포를 주입하는 것보다는 5일 후에 주입하는 것이 척수기능을 회복시키는데 더 효과적이라고 판단할 수 있다. 이상의 결과로 보아 척수 손상 후 5일에 hUCB세포를 주입한 실험 군이 행동평가는 물론 면역형광염색과 조직화학염 색에서도 가장 좋은 양상을 보이는 결과를 얻을 수 있었다. Many approaches have been adopted to restore function following spinal cord injury (SCI). These have included transplantation of fetal neurons, neuronal progenitor cells, or glial cells, or transplantation of transfected cells which produce a variety of substances. The use of human umbilical cord blood cells (hUCB cells) has recently been reported to alleviate behavioral consequences of stroke injury. We report here that hUCB cells delivered intravenously in rats with compression injury of the spinal cord increase the rate behavioral recovery. Tweny-five rats were divided into 5 groups (laminectomy only, laminectomy±hUCB cells, SCI±hUCB cells devlivered at one day post-injury, SCI±hUCBcells delivered at 5 days post-injury, and SCI only). SCI was produced by compressing the spinal cord for one minute with an aneurysm clip calibrated to a closing pressure of 50 gms. Rats were assessed behaviorally at one, two and three weeks using the BBB behavioral scale, inclined platform, and extension and toe spread tests. Following behavioral testing, spinal cords from these rats were examined immunohistochemically to identify hUCB cells. Spinal cords from SCI±hUCB cells animals contained hUCB cells in the area of SCI: No hUCBcells cells were found in noninjured areas of spinal cord from these animals or in animals in which only a laminectomy was performed. Rats in the SCI±hUCBcells 1 day group were significantly different in recovery of motor function as compared to the SCI±hUCB cells 5 day group and laminectomy groups. By three weeks, SCI±hUCB cells animals were not significantly different from each other. These results indicate that hUCBcells cells may be beneficial in reversing the behavioral effects of SCI.

식물의 구조와 기능'에 대한 초등학교 아동들의 지식구조와 학습성향과의 관계 : Reflected on the Concept Maps of the 'Structure and Function of Plant' Unit

김종중,송남희 한국과학교육학회 2002 한국과학교육학회지 Vol.22 No.4

본 연구는 '식물의 구조와 기능'단원의 학습 전 아동의 지식구조와 학습과정 및 학습 종료 7개월 후까지의 지식구조의 변화를 아동의 학습성향(유의미학습과 암기학습)과 관련지위 조사한 것이다. 연구단원과 관련된 기존의 지식구조를 알아보기 위하여 5학년 아동들에게 1차 개념도를 그리게 하고, 매 4차시 수업 후각각의 개념도를 그리게 했다. 그리고 지식구조의 지속효과를 알아보기 위하여 단원 학습이 종료된 직후와 3개원, 7개월 후에도 각각의 개념도를 그리게 했다. 학습과정 동안에는 기존의 개념도를 근거로 하여 개념도를 그리게 하였으며, 단원 학습 종료 후에는 기존의 개념도가 제시되지 않은 상태에서 개념도를 그리게 하였다. 아동들이 그린 각각의 개념도는 그 당시의 식물의 구조와 기능에 대한 이해 정도를 나타내었다. 초등학교 5학년 아동들은 '식물의 구조와 기능' 단원의 학습에 들어가기 전에 이와 관련된 여러 가지 개념들로 이루어진 사전 지식구조를 가지고 있으나 그 중 과학적으로 타당한 것은 개념도 상에서 2단계의 위계와 5개의 관계에 불과하였다. 한 단원의 학습이 진행되어감에 따라 아동들의 지식구조는 꾸준히 그리고 점진적으로 증가였다. 그러나 이러한 증가는 학습성향에 따라 차이를 보이며, 유의미 학습자가 암기 학습자보다 더 많이 증가하였다(관계, 연관, 예:p<.01. 위계: p<.05). 지식구조의 변화에 있어서 학습성향간에 다소 차이가 있으나 재구조화는 단원 학습성향간에 다소 차이가 있으나 재구조화는 단원학습의 초기에 높은 빈도로 일어났으며, 말기에는 전혀 일어나지 않았다. 조정은 유의미 학습자보다 암기 학습자에서 더 많이 일어나고 확장은 이와 반대로 유의미 학습자에서 더 많이 이어났다. 암기 학습자의 개념도 점수는 단원 학습을 마친 후 시간이 경과함에 따라 모든 범주에서 차츰 감소하였다. 그러나 유의미 학습자의 경우 단원 학습 후 3개월보다 7개월에서 오히려 소폭 증가했다. 그러므로 대체로 암기 학습자가 학습과정에서 새로이 구축하고자 하는 더 분화되고 정교한 지식구조가 유의미 학습자보다 불안정한 상태에 있으며, 지식구조의 분화 정도도 유의미 학습자에 비해 낮다고 할 수 있다. This study examined the knowledge structure constructed by children before formal instruction, and successive changes in the structural complexity of knowledge during and after the learning of 'Structure and Function of Plant' unit. It also investigated how those changes were affected by children's learning modes. The researchers made the 5th graders draw the first draft of their concept map to see the pre-existing knowledge structure concerned with the unit and four more concept maps after completing every fourth lesson. And to see how long their knowledge structures were preserved, the researchers made children draw additional concept maps in 3 days, 3 months, and 7 months after completing the unit. Children drew their current concept maps on the basis of the previous one while learning the unit and without the previous one after completing the unit. Each concept map drawn by children showed the degree of their current understanding on the structures and functions of plants. The results revealed that only two levels of hierarchy and five relationships among the components of the first concept map(relationship, hierarchy, cross link and example) were proven to be valid in terms of conceptual relevance. Growth in the structural complexity of knowledge took place progressively throughout the unit and the effects of learning mode on the growth were favorably reflected in concept map scores of meaningful learners over time(relationship, cross link, example: p<.01, hierarchy: p<.05). Although there were some differences on the concept map scores between two types of learners, they commonly showed that knowledge restructuring had occurred apparently in the early periods from the 1st to the 6th lesson and had not occurred at all in the last period of the unit. The frequency of tuning was higher in rote learners than in meaningful learners throughout the unit, but the frequency of accretion was reverse. Concept map scores of rote learners constructed in the course of learning of the unit decreased little by little gradually in all the categories after completing the unit. However, the average total map score of meaningful learners increased a little more in 7 months than in 3 months after completing the unit. Therefore it can be inferred that meaningful learners construct more stable and well-differentiated knowledge structures than the rote learners.

도시 첨단산업클러스터의 입지요인 분석

김종중 대한국토·도시계획학회 2009 國土計劃 Vol.44 No.2

This study aims to analyze a spatial concentration and network of Seoul Digital Valley as a representative case of urban industrial complex in Korea and secondly, to identify locational factors of Seoul Digital Valley and finally to suggest a policy implication. Also this study extract variables affecting business' decision to stay or leave to other areas and interpret its meaning. The frequency of firms surveyed by business type, business scale, development phase etc is analyzed. The forward and backward linkage of planning-R&D-production-marketing are also analyzed. And also the location of R&D service is almost in Seoul Digital Valley. The results show that the businesses in Seoul Digital Valley are R&D-oriented small and medium enterprises. The frequency and the magnitude of the locational advantages is analyzed. The result shows that the advantages responded frequently are as follows: location cost, transportation infrastructure, service facilities, pleasant environment, telecommunication infrastructure, accessibility to urban center or major facilities. This show the result of factor analysis on locational advantages to extract new factors representing the similar group of locational advantages. There are five factors which has eigen value over 1.0. And those five factors can explain 62.8% of all variance. Five factors could be named as locational factor of business support, locational factor of physical facilities, locational factor of environment, locational factor of business clustering, locational factor of support policies. The probability of continuous stay is analyzed to find out which locational advantage affect the decision on stay or not of the firms in Seoul Digital Valley. The result of logistic regression shows that the variables positively affecting the decision of remaining are accessibility to customers and market, concentration of the same business type for technology cooperation, technology cooperation with a higher research and education institute, easiness of using technology support institute, support policy or program of local government, easiness of expansion, brand value of Seoul Digital Valley, transportation easiness to related firms, telecommunication infrastructure, linkage with R&D and production, accessibility to urban center or major facilities, service facilities. The result of logistic regression shows that the variables negatively affecting the decision of remaining are accessibility to suppliers, easiness of securing a high class work force, concentration of business support service, enlargement of market, regional quality for innovation and cooperation, support policy or program of central government, plenty of learning opportunity, location cost, transportation infrastructure, pleasant environment. In summary, the locational advantages of Seoul Digital Valley are formed focusing on physical and environmental factors and these could induce high technology firms to Seoul Digital Valley. But the support on technology innovation and R&D related innovation is necessary to upgrade the competitiveness of Seoul Digital Valley.

식물의 구조와 기능 단원의 학습과정에서 초등학교 아동들의 지식구조의 변화

김종중(Jong Jung Kim),송남희(Nam Hi Song) 한국초등과학교육학회 2002 초등과학교육 Vol.21 No.1

This study examined the knowledge structure constructed by children before formal instruction, and successive changes in the structural complexity of knowledge during the learning of the `Structure and Function of Plants` unit. The researchers let the 5th graders draw the first draft of their concept maps in order to examine the pre-existing knowledge structure concerned with the unit and also four concept maps after completing every fourth lesson. Each concept map drawn by children on the basis of the previous one showed the degree of their current understanding on the structure and function of plants. The results revealed that only two levels of hierarchy and five relationships among the components of the first concept map(relationship, hierarchy, cross link and example) were proven to be valid in terms of conceptual relevance. According to the standard map, there was no change in hierarchy from the 2nd to the 3rd map, and in example from the 2nd to the 4th map. However, the gradual and successive increases of the scores in all components appeared in the children`s maps throughout the unit. Knowledge restructuring occurred strongly in the early periods from the 1st to the 6th lesson, and the significant stable changes in tuning and accretion appeared throughout the whole lessons. The results also showed that there were no significant gender differences on the 5th grader`s knowledge structuring.