염증성 치은조직과 치주인대세포에서 Cytokine에 의해 유도되는 열충격단백 발현에 관한 연구

조인호,김덕규,김은철,유형근,신형식,Cho, In-Ho,Kim, Doek-Kyu,Kim, Eun-Cheol,You, Hyung-Keun,Shink, Hyung-Shin 대한치주과학회 1998 Journal of Periodontal & Implant Science Vol.28 No.1

Prokaryotic and eukaryotic cells respond to heat stress and other environmental abuses by synthesizing a small set of stress proteins and by inhibiting post-transcription synthesis of normal proteins. The purpose of the present study was to document the stress response produced by inflamed gingival tissue in vivo, and cytokine inducted human periodontal ligament cells. Human PDL cells were exposed to TNF-$\alpha$(1ng/ml), INF-$\gamma$(200 U/ml), LPS(100ug/ml), combination of cytokine, and SDS-PAGE gels running and Western blotting analysis was done. In vivo studies, the healthy gingival tissusse of a control group and inflamed gingival tissue of adult periodontitis were studied by immunohistochemistry and histology. The results were as follows 1. HSP 47 was distributed on basal layer in healthy gingiva, but stronger stained in basal, suprabasal, and spinous layer of inflamed gingiva. 2. HSP 47 was rare on endothelial cells and mononuclear cells in healthy gingiva, but stronger expressed in inflamed gingiva. 3. HSP 70 expression was rare on epihelium and inflammatory cells hi both healthy & inflamed gingiva. 4. HSP 70 was actively expressed on endothelial cells and inflammatory cells of capillary lumen in moderately & mild inflamend gingiva. 5. PDL cells showed low level of HSP 47 protein expression which was significantly induced by cytokine stimulation (LSP only and combination). 6. Maximum HSP 70 protein induction was seen with stimulation by a combination of the cytokine, Combination of TNF-$\alpha$, INF-$\gamma$, LPS have been shown to synergistically effects of HSP 70 expression. On the above findings, HSP Is influenced by cytokine and chronic inflammation in vivo, and may be involved in protection of tissue during periodontal inflammatiom.

난소절제로 유도된 골다공증 흰쥐에서 implant 주위 조직 반응에 관한 실험적 연구

조인호,김종여,박수성,박종섭,임헌송,Cho, In-Ho,Kim, Jong-Yeo,Park, Su-Seong,Park, Jong-Sup,Lim, Heon-Song 대한치과보철학회 1998 대한치과보철학회지 Vol.36 No.1

This study was designed to investigate the peri-implant tissue reaction in ovariectomized osteoporotic female rats, and to evaluate effects of estrogen, calcitonin, parathyroid hormone on the bone - implant interface in osteoporotic rats. 120 Sprague - Dawley rats were used in this experiments. Osteoporosis was induced by bilateral ovariectomy. They were divided 5 groups : sham-operated control group(Sham), ovariectomized group (OVX), OVX and estrogen treated group (OVX+E), OVX and PTH treated group (OVX+PTH), and OVX and calcitonin treated group (OVX+CT). Eight weeks after ovariectomy, two titanium screw implants were inserted into the left tibia of each rat. Eight weeks after the insertion of the implants, the periotest values (PTV) of implant were examined, and the rats were sacrificed, and examined the reaction of bone tissue surrounding the implant both histologically and histomorphometrically. The bone density and ash weight of opposite right tibia were examined. Over 40 rats were fractured on left tibia that was implant inserted. On histologically finding, all groups were osseointegrated well, especially in OVX+PTH group. In OVX group, tibial cortical bone showed many large harversian canal and microfracture lines. The OVX+PTH group showed the lowest mean PTV (-2.33) (p<0.05), and the hightest mean bone - implant contact percentage (89%) (p>0.05). But the OVX+CT group showed the highest mean bone density ($5.45mg/cm^3$) and ash weight (56.12%) (p<0.05). The results indicate that PTH treatment enhances osseointegration of implant in OVX rats, and CT treatment depresses bone turnover and prevent the development of osteopenia in OVX rats.

CAD/CAM을 이용하여 제작한 All-ceramic core의 변연 적합도

김동근,조인호,임주환,임헌승,Kim Dong-Keun,Cho In-Ho,Lim Ju-Hwan,Lim Heon-Song 대한치과보철학회 2003 대한치과보철학회지 Vol.41 No.1

Novel methods producing supplementary and prosthetic material by cutting or discharge processing via computer design have been proposed as alternatives for traditional casting methods and are being utilized for commercial purposes. The CAD/CAM system used in dentistry can be classified into three-dimensional input of target values, restoration design, and material processing. The marginal fidelity in production of In-Ceram core has important clinical implications and is a key consideration issue in CAD/CAM production as well. Through this research, the author arrived at the following conclusion aaer conducting comparison analysis of marginal fidelities between the In-Ceram core produced via CAD/CAM and that produced through the traditional method ; 1. In the cases of mesial, distal, and lingual margins, the core margins via CAD/CAM produced lower values than those via the traditional method, but the differences were found to be statistically insignificant. 2. In the case of labial flange, the core margins via CAD/CAM produced lower values than those via the traditional method and the differences were found to be statistically significant. (p<0.05) 3. In comparision with overall marginal fidelity, the core margins via CAD/CAM produced lower values than those via the traditional method, but the differences were found to be statistically insignificant. 4. Among the core margins produced via the traditional method did not have statistically significant differences but fir those produced via CAD/CAM had statistically significant differences between labial and lingual sides and between labial and mesial sides. (p <0.05).

공진 주파수 분석법에 의한 임플랜트의 안정성 측정에 관한 연구

박철,임주환,조인호,임헌송,Park Cheol,Lim Ju-Hwan,Cho In-Ho,Lim Heon-Song 대한치과보철학회 2003 대한치과보철학회지 Vol.41 No.2

Statement of problem : Successful osseointegration of endosseous threaded implants is dependent on many factors. These may include the surface characteristics and gross geometry of implants, the quality and quantity of bone where implants are placed, and the magnitude and direction of stress in functional occlusion. Therefore clinical quantitative measurement of primary stability at placement and functional state of implant may play a role in prediction of possible clinical symptoms and the renovation of implant geometry, types and surface characteristic according to each patients conditions. Ultimately, it may increase success rate of implants. Purpose : Many available non-invasive techniques used for the clinical measurement of implant stability and osseointegration include percussion, radiography, the $Periotest^{(R)}$, Dental Fine $Tester^{(R)}$ and so on. There is, however, relatively little research undertaken to standardize quantitative measurement of stability of implant and osseointegration due to the various clinical applications performed by each individual operator. Therefore, in order to develop non-invasive experimental method to measure stability of implant quantitatively, the resonance frequency analyzer to measure the natural frequency of specific substance was developed in the procedure of this study. Material & method : To test the stability of the resonance frequency analyzer developed in this study, following methods and materials were used : 1) In-vitro study: the implant was placed in both epoxy resin of which physical properties are similar to the bone stiffness of human and fresh cow rib bone specimen. Then the resonance frequency values of them were measured and analyzed. In an attempt to test the reliability of the data gathered with the resonance frequency analyzer, comparative analysis with the data from the Periotest was conducted. 2) In-vivo study: the implants were inserted into the tibiae of 10 New Zealand rabbits and the resonance frequency value of them with connected abutments at healing time are measured immediately after insertion and gauged every 4 weeks for 16 weeks. Results : Results from these studies were such as follows : The same length implants placed in Hot Melt showed the repetitive resonance frequency values. As the length of abutment increased, the resonance frequency value changed significantly (p<0.01). As the thickness of transducer increased in order of 0.5, 1.0 and 2.0 mm, the resonance frequency value significantly increased (p<0.05). The implants placed in PL-2 and epoxy resin with different exposure degree resulted in the increase of resonance frequency value as the exposure degree of implants and the length of abutment decreased. In comparative experiment based on physical properties, as the thickness of transducer increased, the resonance frequency value increased significantly(p<0.01). As the stiffness of substances where implants were placed increased, and the effective length of implants decreased, the resonance frequencies value increased significantly (p<0.05). In the experiment with cow rib bone specimen, the increase of the length of abutment resulted in significant difference between the results from resonance frequency analyzer and the $Periotest^{(R)}$. There was no difference with significant meaning in the comparison based on the direction of measurement between the resonance frequency value and the $Periotest^{(R)}$ value (p<0.05). In-vivo experiment resulted in repetitive patternes of resonance frequency. As the time elapsed, the resonance frequency value increased significantly with the exception of 4th and 8th week (p<0.05). Conclusion : The development of resonance frequency analyzer is an attempt to standardize the quantitative measurement of stability of implant and osseointegration and compensate for the reliability of data from other non-invasive measuring devices It is considered that further research i

항암항생제 Daunorubicin의 Aglycone, 7,9-Dideoxydaunomycinone의 합성 (제1보)

조인호,이풍래,노영쇠,In Ho Cho,Richard P. Rhee,Young Soy Rho,F. M. Hauser 대한화학회 1986 대한화학회지 Vol.30 No.1

항암항생제 Daunorubicin(2a)합성단계의 최종물질인 7,9-Dideoxydaunomycinone (32)를 3-methoxybenzoic acid(5)로 부터 합성하였다. 3-methoxybenzoic acid를 4-methoxy-3-(phenylsulfonyl)-1(3H)-isobenzofuranone(11)으로 변형시킨 뒤 Hauser와 Rhee가 개발한 고리접합법을 이용하여 trimethoxynaphthoate 16을 얻은 후에 phenylsulfonylnaphthofuranone 22로 변화시킨 뒤 이 물질을 7-(ethylenedioxy)-2-octenoate(23)과 Michael형태의 반응을 전개시켜 anthracenoate 24를 얻었다. Anthracenoate을 tetracyclic 화합물 28로 바꾼뒤 ring B와 C에 붙은 methyl기들을 제거하여 7,9-Dideoxydaunomycinone(32)를 만들었다. 7,9-Dideoxydaunomycinone (32), a late-stage precursor of the aglycone of antitumor antibiotic daunorubicin(2a) was prepared from 3-methoxybenzoic acid(5). Thus, 3-methoxybenzoic acid was converted to 4-methoxy-3-(phenylsulfonyl)-1(3H)-isobenzofuranone(11), which furnished trimethoxynaphthoate 16 upon ring annelation developed by Hauser and Rhee. The trimethoxynaphthoate 16 upon ring annelation developed by Hauser and Rbee. The trimethoxynaphtboate 16 was then transformed into phenylsulfonylnaphthofuranone 22, which was used to make anthracenoate 24 via Michael type reaction with 7-(ethylenedioxy)-2-octenoate(23). Conversion of anthracenoate 24 to tetracyclic product 28, followed by subsequent deprotection of the methyl groups in ring-B and C furnished 7, 9-Dideoxydaunomycinone(32).

9,10-Dideoxy-${\beta}$-rhodomycinone의 합성

조인호,노영쇠,소상문,유동진,이준용,한병구,In Ho Cho,Young Soy Rho,Sang Moon Sho,Dong Jin Yoo,Jun Yong Lee,Byoung Ku Han 대한화학회 1992 대한화학회지 Vol.36 No.6

${\beta}$-rhodomycinone(1)의 전 단계물질인 9,10-dideoxyrhodomycinone(30)의 전 합성이 이루어졌다. Phthalide sulfone 4를 몇 단계의 반응을 진행시켜서 naphthalide sulfone 12로 변형시킨 뒤, 음이온으로 변형된 naphthalide sulfone 12를 5-ethyl-1,3-cyclohexenone(21)과 Michael 부가반응시켜서 선형으로 결합된 tetracyclic 케톤 화합물 26을 좋은 수율로 얻었다. 화합물 26의 케톤기는 sodium borohydride로 환원시키고 메틸기로 보호하여 화합물 28을 얻었다. 9,10-Dideoxy-${\beta}$-rhodomycinone(30)은 pentamethoxy tetracyclic 화합물 28을 oxidative demethylation시키고 메틸기를 제거시켜서 합성하였다. Total synthesis of 9,10-dideoxyrhodomycinone(30) which is the late-stage precursor of naturally occuring ${\beta}$-rhodomycinone(1) is described. After phthalide sulfone 4 was converted to naphthalide sulfone 12 by the several step. Michale addition of naphthalide sulfone 12 which was converted to an anion with 5-ethyl-1,3-cyclohexenone(21) gave a good yield of linearly condensed tetracyclic ketone compound 26. The keto group of 26 was reduced with sodium borohydride and protected by methyl group to afford compound 28. 9,10-Dideoxy-${\beta}$-rhodomycinone(30) was synthesized from pentamethoxy tetracyclic compound 28 by oxidative demethylation and demethylation of 29.

항암항생제 6-Deoxybisanhydrodaunomycinone의 합성

조인호,노영쇠,박시호,안구현,신홍식,한병구,Cho, In Ho,Rho Young Soy,Park, Si Ho,Ahn Koo Hyeon,Sin Hong Sig,Han Byoung Ku 대한화학회 1993 대한화학회지 Vol.37 No.1

항암항생제 Danuorubicin(1b)의 aglycone인 daunomycinone의 전이물질 6-deoxybisanhydrodaunomycinone(20)의 전 합성이 이루어졌다. 만들어진 enone 화합물 4를 phthalide sulfone 7과 반응시킨 뒤 oxidation과 methylation을 시켜서 anthraquinone 화합물 10을 얻었다. 화합물 10의 benzyl기를 bromination시켜서 얻은 monobromo 화합물 11을 bis(tetrabutylammonium) dichromate로 고리화반응을 시켜서 hydroxyanthraquinone 화합물 16을 얻은 뒤 OH기를 thiophenol로 치환시켰다. sulfide 화합물 17은 phosphate buffer 용액속에서 m-CPBA로써 산화시켜서 anthraquinonyl sulfone 화합물 18을 얻은 뒤 methyl vinyl ketone(19)과 결합시켜서 화합물 20을 얻었다. A brief route for total synthesis of 6-deoxybisanhydrodaunomycinone(20) was described, namely the precursor of the daunomycinone, the aglycone of the anticancer antibiotic daunorubicin (1b). The prepared enone 4 was condensed with phthalide sulfone 7 to afford anthraquinone 10 after oxidation and methylation. The benzylic group of 10 was brominated, and subsequent oxidation with bis(tetrabutylammonium) dichromate followed by cyclization give hydroxyanthraquinone 16, which was displaced with thiophenol. Oxidation of 17 with m-CPBA in phosphate buffer solution afforded anthraquinonyl sulfone 18 which was condensed with methyl vinyl ketone (19) to furnish 20.

연구발표논문초록 ( 공정시스템 (Ⅰ) ) : 다단 증류탑의 최적 설계에 관한 연구

조인호,윤인섭 ( In Ho Cho,En Sup Yoon ) 한국화학공학회 1988 춘계학술발표회 Vol.- No.-

항암항생제 Aklavin의 7-Deoxyaklavinone 합성

조인호,정진순,한병구,유동진,이준용,노영소,In Ho Cho,Jin Soon Chung,Byoung Ku Han,Dong Jin Yoo,Jun Yong Lee,Young Soy Rho 대한화학회 1989 대한화학회지 Vol.33 No.6

Phthalide sulfone 2와 naphthalenone 6을 만들어서 Hauser-Rhee가 개발한 Michael addition의 새로운 고리 접합법으로 4개의 선형 고리화합물의 구조를 갖는 tetracyclic 화합물 11을 만들었다. 화합물 11의 ring A가 갖고 있는 중요한 이중결합을 이용해서 탄소수가 한 개 증가된 carbomethoxy 화합물 16으로 변형시키는 것은 Arndt-Eistert, Wolff rearrangement를 이용하였다. 그리고 나서 이들을 고리화 반응시킨 뒤 hydroxylation시켜서 최종 생성물인 (${\pm}$)-7-Deoxyaklavinone(18)를 합성하였다. Syntheses of phthalide sulfone 2 and naphthalenone 6 followed by new ring annelation methodology of Michael addition using the sulfone anion developed by Hauser-Rhee, furnished linear tetracyclic ring system compound 11. The double bond existing in A-ring of 11 was used to convert to carbomethoxy compound 16, possessing one carbon atom more via Arndt-Eistert synthesis and Wolff rearrangement. Cyclization and hydroxylation of 16 completed the construction of (${\pm}$)-7-Deoxyaklavinone (18).

총의치 인상

조인호,Cho, In-Ho 대한치과보철학회 1989 대한치과보철학회지 Vol.27 No.1