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田暢淇,石島芳郞 충남대학교 농업과학연구소 1978 農業技術硏究報告 Vol.5 No.2
The Present study was examined the response of mice of 5 strains to superovulation treatment. Female mice of CF #1, C57BL/6, ICR, dd and CHN strains, aged 60 days were used. Superovulation was induced by the subcutaneous injection of 5 IU PMS and followed 48 hours later by the subcutaneous injection of 5 IU HCG. All females were killed 24 hours after HCG and then ova in the oviduct were counted under a microscope. The average number of ova ovulated in CF #1, C57BL/6, ICR, dd and CHN were 21.3, 17.9, 20.7, 21.0 and 20.5, respectively.
過排卵處理 마우스에 있어서의 排卵數의 左右分布에 관한 硏究
田暢淇,石島芳郞 충남대학교 농업과학연구소 1977 農業技術硏究報告 Vol.4 No.1
The distribution of number of ovulation between right and left ovary in superovulated mice were examined. 50 adult female mice of CF#1 strain were used in this study. Superovulation was induced by the subcutaneous injection of 5 IU PMS and followed 48 hours later by the subcutaneous injection of 5 IU HCG. Females a were killed at 24 hours after injection of HCG, and ova in the oviducts were counted under a microscope. The number of ova ovulated from the left and the right ovary were 603 and 594. The number of ova in both sides were found equal frequency by X^2 test method. Ovulation occurred more frequently in the left ovary than in the right in 24 females and with equal frequency in both ovaries in 3 females, while more frequently in the right ovary than in the left in 23 females. The possitive correlation was found between right and left ovary.
전창기,석도방랑 ( C . G . Jeon ) 한국축산학회 1974 한국축산학회지 Vol.16 No.4
This experiment was studied of the peritoneal fluid when it was used for culture fluid of fertilized ova of rabbits and it was cultivated in vitro. The peritoneal fluid also can be gained much from the superovulated rabbits. The results were as follow. 1. When 2, 4, 8 cell stage ova of rabbit were cultivated in peritoneal fluid at 37℃ for 48 hours each ova was developed as much as 78.1%, 83.3% and 91.7%, and these records were superior to the useing of blood serum. 2. When 2 cell stage ova of rabbit was cultivated in peritoneal fluid, 88% of ova were rapidly growth for morula stage and 24% became blastcyst stage. These records are nearly same to the records in blood serum. At these points, the rabbit peritoneal fluid can be used effectively for ova culture fluid.
전창기,석도방랑 ( C . G . Jeon ) 한국축산학회 1974 한국축산학회지 Vol.16 No.4
This experiment was studied on the times of fetus-death during the gestation period of superovulated mows, and inspectation of implantation and living fetus number and the implantation points were done after 10, 12, 14, 16, 18 days from gestation. The results are as follows: 1. The coputaion rate was 68.1% (47/69) and the gestation rate was 68.1% (32/47) of superovulated mouse. 2. The average number of implantation was resulted like these, the 10 days after was 20.8, 12days after 18. 2, 14 clays after 17.1, 16 days after 17.0 and 18 days after 19.0, while the average number of living fetus were 10 days after 20.8 (100%: living fetus number/implantation number), 12 days after 16.3 (89.9%), 14 days after16.9 (98.3%), 16 days after 10.7 (62.7%) and 18 days after 10.2 (53.7%), and living fetus number was rapidly decreased from 16 days over. 3. The litter size of superovulated mouse was average 6.9 heads and did not over the control plot of which number is aveage 9.7 heads.