Chlortetracycline Fluoresence 분석을 통한 수정능 획득 과정에서의 $Ca^{2+}$-ATPase 역할

박경식,Park, Kyoung-Sik 대한생식의학회 1998 Clinical and Experimental Reproductive Medicine Vol.25 No.3

It has been reported that the $Ca^{2+}$-ATPase and the $Ca^{2+}-Na^+$ exchanger play an important role for the regulation of intracellular $Ca^{2+}$ in somatic cells, the $Ca^{2+}$-ATPase located in the plasma membrane helps the $Ca^{2+}$ concentration in maintain low $[Ca^{2+}]_i$. Roldan & Fleming reported that the spermatozoan $Ca^{2+}$-ATPase plays an important role in the capacitation and acrosome reaction. We used to assess $Ca^{2+}$ changes by chlortetracycline (CTC) patterns in the capacitation and acrosome reaction of human and hamster spermatozoa. In the present study applying quercetin which has been known as an ATPase antagonist, the enzymatic effect of $Ca^{2+}$-ATPase on capacitation and acrosome reaction was found to be remarkable: a significant increase of the transformation from the original type to the B type and the AR type of spermatozoa. This finding suggests that $Ca^{2+}$-ATPase play an important role in the efflux and the influx of the $Ca^{2+}$ which have been known to be an essential factor for the capacitation and acrosome reaction, and that the inhibitory action of the $Ca^{2+}$-ATPase might be a prerequsit step toward the capacitation and acrosome reaction. In conclusion, this study suggest the considerable evidence as follows: the increment of the intracellular $Ca^{2+}$ concentration occurred by controlling the slope of $Ca^{2+}$ concentration through $Ca^{2+}$-ATPase activites in both the intracellular and extracellulr fluid may be important procedures for the capacitation and the acrosome reaction, and finally for fertilization of the sperm and ovum.

흰쥐에 있어서 LY-117018 및 Tamoxifen이 생식기관에 미치는 영향

박경식,권종국,Park, Kyoung-Sik,Kwun, Jong-Kuk 대한생식의학회 1987 Clinical and Experimental Reproductive Medicine Vol.14 No.1

This study was carried out to investigate the effects of the antiestrogens, LY-117018 and tamoxifen on reproductive organ of ovariectomized immature rats and also to elucidate the mechanism of action of said compounds by bioassay. Each of LY-117018, tamoxifen and estradiol-17${\beta}$ was administered to ovariectomized immature rats at various dose levels. Forty hours after drug administration, tested rats were sacrificed and uterine wet weight, DNA and RNA contents in uterine and liver tissues were investigated. At the same time, uterine wet weight was also investigated with some other rats treated with 125${\mu}g$ of LY-117018 together with increasing doses of tamoxifen. Ovariectomized immature rats given 25${\mu}g$ single dose of each drug were sacrificed on Day 1, 2, 3, 4, and 5 after drug administration and uterine was weighed to estimate the duration of action of LY-117018 and tamoxifen. The results were summarized as follows: 1. The administration of LY-117018 or tamoxifen to ovariectomized rats increased uterine wet weight and DNA and RNA contents in uterine tissues with more increase in tamoxifen groups, but significant differences between groups treated at dose levels of 5${\beta}$ or more of both drugs were observed. Estradiol-17${\beta}$ groups showed significant increases in each group(P<0.01). 2. The administration of LY-117018 or tamoxifen to each group significantly increased DNA and RNA contents in liver tissues with more increase in tamoxifen groups. Estradiol-17${\beta}$ groups showed no significant differences between treatment groups of 5${\beta}$ or more. 3. Treatment with 125${\beta}$ of LY-117018 together with various doses of tamoxifen resulted in more increase of uterine wet weight than treatment with a single dose of LY-117018 or tamoxifen. 4. Treatment with 0.2${\beta}$ of LY-117018 or tamoxifen in ovariectomized rats decreased uterine wet weight,DNA and RNA contents in liver and uterine tissues compared with ovariectomized control. 5. The duration of effective action of LY-1l7018 and tamoxifen was 4 days or more. 6. There was significant difference(P<0.001) in uterine wet weight between Day 9after ovariectomy (two days after LY-117018 or tamoxifen treatment) and Day 10(63.7${\pm}$3.5mg, 39.2${\pm}$9.9mg, respectively).

흰쥐에 있어서 LY-l17018 및 tamoxifen이 이 착상에 미치는 영향

박경식,권종국,Park, Kyoung-Sik,Kwun, Jong-Kuk 대한생리학회 1986 대한생리학회지 Vol.20 No.2

These studies were carried out to investigate the effects of the antiestrogens, LY-117018 and tamoxifen, on implantation in ovariectomized or intact adult rats. A Quantity of $80\;{\mu}g$ of LY-l17018 or tamoxifen was given to adult female rats on Day 1, 2, 3 and 4 of pregnancy and investigated the implantation sites on Bay 8 of pregnancy. The rats were ovariectomized at the first day of pregnancy and treated with various doses of LY-l17018 or tamoxifen together with progesterone daily from Day 2 to 8 of pregnancy and then investigated the implantation sites on Day 8 of pregnancy The results were summarized as follows; When a single dose of $80\;{\mu}g$ LY-l17018 and tamoxifen was given during the first 4 days of pregnancy, the implantation was intesively inhibited in the pregnant rat treated with LY-l17018 on Day 2 $(14.4{\pm}3.5%),\;3(16.3{\pm}5.3%)\;and\;tamoxifen\;on\;Days\;2\;(17.4{\pm}4.6%),\;3\;(16.3{\pm}2.8%)\;and\;4\;(13.9{\pm}3.5%).$ LY-l17018 was apt to inhibit more potently the implantation than tamoxifen except on Day 4 of pregnancy In rats ovariectomized on Day 1 of pregnancy and treated continucusly with 12? r9 of LY-117018 and tamoxifen together with progesterone showed the highest implantation rate, compared with the rats treated continuously with different doses of the two drugs. The correlation coefficients between the dosage of drugs and implantation rate were r= 0.91 (LY-117018), 0.51 (tamoxifen), respectively, except treatment with $625\;{\mu}g$ of the drugs. Tamoxifen was apt to stimulate the implantation more potently than LY-l17018 except groups treated with $625\;{\mu}g$ of the two drugs.

자침이 뇌에 미치는 영향

박경식,Park Kyoung-Sik 대한예방한의학회 2000 대한예방한의학회지 Vol.4 No.2

This study was carried to identify whether acupuncture of several acupuncture points can affect the brain and to observe which aspects appear in EEG mapping, using electroencephalography. Those results are as follows ; 1. The pattern of resting computerized EEG map in intact human is appered normal 2. Each Acupuncture in Kwan Weon or Jog Sam Ri meridian points bring about the increase in $\theta,\;\alpha-wave$ activity and at various area of the cerebrium and the decrease in $\delta,\;\beta-wave$ activity. It strands to reason that brain function is elevated On the other hand , synchronous acupuncture bring about the decrease of brain function in view of the decrease of $\delta,\;\theta-wave$ activity at frontal area, and the unstable brain state in view of the increase of $\beta-wave$ activity. 3. Acupuncture in Hyeon Jong meridian point bring about the increase of $\delta,\;\theta-wave$ activity at frontal area and $\beta-wave$ activity at temporal area. From these we deduce that brain function is declined and brain is unstable. Synchronous acupuncture with other meridian points reversly showed that brain function is elevated. 4. Synchronous acupuncture in Kwan Weon , Jog Sam Ri, Hyeon Jong bring about the decrease of the brain function and the unstable brain state, showing the pattern of increased $\delta,\;\theta-wave$ activity at frontal, parietal area, and increased $\beta-wave$ activity at temporal area.

수태양소장경근(手太陽小腸經筋)의 해부학적(解剖學的) 연구(硏究)

박경식,Park, Kyoung-Sik 대한약침학회 2004 Journal of pharmacopuncture Vol.7 No.2

This study was carried to identify the component of Small Intestine Meridian Muscle in human, dividing the regional muscle group into outer, middle, and inner layer. the inner part of body surface were opened widely to demonstrate muscles, nerve, blood vessels and the others, displaying the inner structure of Small Intestine Meridian Muscle. We obtained the results as follows; 1. Small Intestine Meridian Muscle is composed of the muscle, nerve and blood vessels. 2. In human anatomy, it is present the difference between a term of nerve or blood vessels which control the muscle of Meridian Muscle and those which pass near by Meridian Muscle. 3. The inner composition of meridian muscle in human arm is as follows ; 1) Muscle ; Abd. digiti minimi muscle(SI-2, 3, 4), pisometacarpal lig.(SI-4), ext. retinaculum. ext. carpi ulnaris m. tendon.(SI-5, 6), ulnar collateral lig.(SI-5), ext. digiti minimi m. tendon(SI-6), ext. carpi ulnaris(SI-7), triceps brachii(SI-9), teres major(SI-9), deltoid(SI-10), infraspinatus(SI-10, 11), trapezius(Sl-12, 13, 14, 15), supraspinatus(SI-12, 13), lesser rhomboid(SI-14), erector spinae(SI-14, 15), levator scapular(SI-15), sternocleidomastoid(SI-16, 17), splenius capitis(SI-16), semispinalis capitis(SI-16), digasuicus(SI-17), zygomaticus major(Il-18), masseter(SI-18), auriculoris anterior(SI-19) 2) Nerve ; Dorsal branch of ulnar nerve(SI-1, 2, 3, 4, 5, 6), br. of mod. antebrachial cutaneous n.(SI-6, 7), br. of post. antebrachial cutaneous n.(SI-6,7), br. of radial n.(SI-7), ulnar n.(SI-8), br. of axillary n.(SI-9), radial n.(SI-9), subscapular n. br.(SI-9), cutaneous n. br. from C7, 8(SI-10, 14), suprascapular n.(SI-10, 11, 12, 13), intercostal n. br. from T2(SI-11), lat. supraclavicular n. br.(SI-12), intercostal n. br. from C8, T1(SI-12), accessory n. br.(SI-12, 13, 14, 15, 16, 17), intercostal n. br. from T1,2(SI-13), dorsal scapular n.(SI-14, 15), cutaneous n. br. from C6, C7(SI-15), transverse cervical n.(SI-16), lesser occipital n. & great auricular n. from cervical plexus(SI-16), cervical n. from C2,3(SI-16), fascial n. br.(SI-17), great auricular n. br.(SI-17), cervical n. br. from C2(SI-17), vagus n.(SI-17),hypoglossal n.(SI-17), glossopharyngeal n.(SI-17), sympathetic trunk(SI-17), zygomatic br. of fascial n.(SI-18), maxillary n. br.(SI-18), auriculotemporal n.(SI-19), temporal br. of fascial n.(SI-19) 3) Blood vessels ; Dorsal digital vein.(SI-1), dorsal br. of proper palmar digital artery(SI-1), br. of dorsal metacarpal a. & v.(SI-2, 3, 4), dorsal carpal br. of ulnar a.(SI-4, 5), post. interosseous a. br.(SI-6,7), post. ulnar recurrent a.(SI-8), circuirflex scapular a.(SI-9, 11) , post. circumflex humeral a. br.(SI-10), suprascapular a.(SI-10, 11, 12, 13), first intercostal a. br.(SI-12, 14), transverse cervical a. br.(SI-12,13,14,15), second intercostal a. br.(SI-13), dorsal scapular a. br.(SI-13, 14, 15), ext. jugular v.(SI-16, 17), occipital a. br.(SI-16), Ext. jugular v. br.(SI-17), post. auricular a.(SI-17), int. jugular v.(SI-17), int. carotid a.(SI-17), transverse fascial a. & v.(SI-18),maxillary a. br.(SI-18), superficial temporal a. & v.(SI-19).

한국의 미숙아 및 정상분만아 산모유의 조성

박경식,강국희 ( Kyung Sik Park,Kook Hee Kang ) 한국낙농학회 1990 韓國酪農學會誌 Vol.12 No.1

한국의 미숙 및 정상분만아의 식이별 (食餌別) 장내균총에 (腸內菌叢) 관한 연구 - II. 식이별 정상분만아의 장내균총의 변화

박경식,강국희 ( Kyung Sik Park,Kook Hee Kang ) 한국낙농학회 1991 韓國酪農學會誌 Vol.13 No.1

Anatomical Study on the Foot Soeum Meridian Muscle in Human

박경식,Park, Kyoung-Sik Society for Meridian and Acupoint 2012 Korean Journal of Acupuncture Vol.28 No.4

본 연구는 족소음경근의 구성요소에 대하여 문헌 해석과 인체의 층별 해부를 통하여 해부학적 관점에서 관찰해 보기 위하여 수행되었다. 그리하여 다음과 같은 결론을 얻을 수 있었다. 1. 본 연구 결과 족소음경근의 구성요소와 분석방법에 있어서 기존의 연구와 다소 차이를 보여준다. 2. 족소음경근의 경로와 병증 증상 등을 고려할 때 족소음경근은 근육, 근막, 인대, 그리고 관련 신경을 포함하는 포괄적 개념으로 보인다. 3. 족소음경근의 분포는 경락과 밀접한 관련성이 있으나 일치하지는 않는 것으로 보이며 특히 치골부위 이상의 부위에선 그러할 것으로 사료된다. 4. 이론적으로 MPS와 Anatomy train 같은 가설과는 약간의 차이가 있어 보이며 굳이 관련시켜 생각할 이유가 없다고 생각한다. Objectives : This study was investigated to observe Foot Soeum Meridian Muscle in human. Methods : In order to expose components related to Foot Soeum Meridian Muscle, cadaver was dissected in the order of their depth; being respectively divided into superficial, middle, and deep layer. Results : Anatomical components related to Foot Soeum Meridian Muscle in human are composed of muscles such as flexor digitorum brevis tendon, abductor hallucis muscle, psoas major m., erector spinae m., and flexor retinaculum, fascia such as plantar aoneurosis, ligament such as sacrotuberal ligament, sacrospinous lig., nuchal lig., nerves such as plantar cut. br. of med. plantar nerve, med. crural cut. br. of saphenous n., br. of tibial n., post. femoral cut. n., spinal n.(dorsal rami of C4-6, T7-12, L1-3, and S1-3), and autonomic nervous system(sacral plexus, pelvic splanchnic n., etc.), and etc. Conclusions : This study shows comparative differences from established studies on anatomical components related to Foot Soeum Meridian Muscle, and the methodical aspects of analytic process. In addition, Foot Soeum Meridian Muscle in human is a comprehensive concept including the relevant nerves, but it remains questionable.

Effect of Platelet-Activating Factor on Cyclic Nucleotide Level in Rat Uterine tissue during Preimplantation Period

박경식,권종국,Park, Kyoung-Sik,Kwun, Jong-Kuk The Korean Society for Reproductive Medicine 1991 Clinical and Experimental Reproductive Medicine Vol.18 No.2

본 연구의 목적은 임신 초기 자궁 조직중의 cyclic nucleotide 의 변화 및 PAF 가 이들에 미치는 영향을 관찰함으로써 PAF 가 흰쥐의 초기 임신에 어떻게 관련하는지를 조사하기 위함이다. 시험구로써 임신 각 일에 $1{\mu}g$ 의 PAF 혹은 이것의 수용체 길항제인 1.25mg의 BN-52021이 근육내 조사되었고 비 임신구 및 대조구에 대하여는 PBS만이 주사되었다. 자궁 조직중의 cAMP 및 cGMP 농도는 분석용 test kit를 사용하여 분석되었다. 비 임신구 경우 자궁 조직중 cAMP 농도는 단백질 mg 당 $2.91{\pm}0.33$ pmol로서 임신 보다도 낮았고 cGMP 농도 또한 $0.39{\pm}0.20$ pmol로서 임신구보다 낮은 경향이 었다. 자궁 조직중 cAMP 의 최고농도는 임신 3일째 ($5.92{\pm}1.72$ pmol/mg protein) 였고 cGMP 경우는 임신 4일째($1.03{\pm}0.22$ pmol/mg protein) 이었다. 임신 각일에 PAF 는 PAF 처리하지 아니한 대조구에 비하여 증가된 cAMP 를 보여주었으나(임신 0, 2, 그리고 4 일째 경우 p<0.05) BN-52021은 감소된 경향을 나타내었다. cGMP에 대하여는 PAF나 BN-52021 공히 일정한 효과적 경향을 보이지 아니하였다. 따라서, 임신은 자궁 조직중 cyclic nucleotide에 영향을 미칠 수 있으며 흰쥐의 착상기동안 PAF는 cGMP에 대하여 보다는 cAMP에 영향을 미침으로써 착상에 관련된 일련의 반응에 영향을 미칠 것으로 사료된다. This study was carried out to observe the change in uterine cyclic nucleotide level and the effect of PAF on cyclic nucleotides in uterine tissue in early pregnany in order to understand reciprocal relation ship between PAF and cyclic nucleotides in pregnancy in the rat. The test groups were injected intramuscularly with $1{\mu}g$ of PAF or 1.25mg of BN-52021 on day 0, 1, 2, 3, 4 and 5 of pregnancy. The level of cyclic nucleotide in removed uterine tissue was assayed by using cyclic nucleotides test kits. The results showed that the cyclic AMP content in uterine tissue of non-pregnant at pro-oestrus rat was $2.91{\pm}0.33$ pmol/mg protein which was lower than those of pregnant rat. The cyclic GMP content in uterine tissue of non-pregnant rat was $0.39{\pm}0.20$ pmol/mg pro-tein which was also lower than those of pregnant rats. The maximum level in cAMP was $5.92{\pm}1.72$ pmol/mg protein on day 3 and cGMP, $1.03{\pm}0.22$ pmol/mg protein on day 4. On each day of pregnancy, PAF induced the increased cAMP level ompared with that of intact rat. That was significant on day 0, 2 and 4 of pregnancy, p<0.05, on the other hand PAF receptor antagonist, BN-52021 ecreased cAMP level in uterine tisssue. PAF as well as BN-52021 had not an consistent effect on changes in cGMP level. These results suggest that cyclic nucleotide levels in uterine tissue ware increased during early pregnancy and PAF influences cAMP level in uterine rather than cGMP level during peri-implantation period, accordingly demonstrating a possible involvement of PAF in the regulation of implantation-related events through cAMP-mediated process.

고유섭과 탑파연구

박경식(Park Kyonngshik) 한국미술사학회 2005 美術史學硏究 Vol.- No.248

일제강점기부터 석탑에 대한 주목이 이루어져 많은 연구가 진행되었는 바, 우리나라 사람으로 이를 최초로 주목한 분이 又玄 高裕燮 선생이다. 선생의 연구는 미학에서부터 탑파, 불상, 도자, 회화에 이르기까지 모든 분야를 망라하고 있다. 하지만, 이 중에서도 하나를 꼽으라면 누구나 탑파연구를 주목함에 주저함이 없을 것으로 생각된다. 선생께서 집필하신 탑파 관련 논고는 모두 11편으로 『韓國美術史及美學論考』, 『韓國塔婆의 硏究』, 『韓國塔婆의 硏究-各論草稿』에 수록되어 있다. 선생의 우리나라 석탑에 대한 연구는 철저한 현지조사와 문헌고증을 바탕으로 양식론과 역사적 상황의 접목은 물론 불교교리에 이르기까지 폭넓게 진행되었다. 뿐만 아니라 우리나라 석탑의 양식 근원은 목탑에 두고 있음을 규명함과 동시에 우리나라 석탑의 발달사를 정립했다. 선생의 탑파연구는 일제강점기 하에서 우리 문화의 암흑기와 같았던 1930년대에 집중적으로 이루어졌기에 그간의 순수학문적인 평가에서 진일보해야 할 것으로 생각한다. 즉 탑파연구를 통해 우리의 문화가 강건한 기반과 오랜 전통을 가지고 있음은 물론, 이를 통해 일본보다 더 우수한 문화가 이 땅에 있음을 당시의 지식층은 물론 일반인들에게 전파했다. 그러므로 선생의 학문세계에 대한 평가는 當代에 활동했던 민족주의 사학자들과 같은 관점에서 평가되어야 할 것으로 생각한다. 즉, 석탑을 비롯한 문화유산을 통해 민족의 기개와 자존심을 높이려 했던 측면에서도 조명되어야 할 것으로 생각한다. The modern scholarship of Korean Buddhist pagodas started in the Japanese colonial period. The first korean who studied stone pagodas was Woohyeon Go Yuseop. He studied various subjects such as aesthetics, stone pagodas, Buddhist sculptures, ceramics and paintings. Among his diverse achievements, the most notable one was on stone pagodas. He wrote many books and articles. Especially, he wrote eleven articles about stone pagodas. They were published in such works as Studies in Korean Art History and Aesthetics (韓國美術史及美學論考), A Study of Korean Buddhist Pagodas (韓國塔婆의 硏究). A Study of Korean Buddhist Pagodas: Preliminary Writings (韓國塔婆의 硏究-各論草稿). He explored in depth stone pagodas. When he wrote on a stone pagoda, he always conducted field survey and examined Buddhist and historic circumstance to support his theory. Also he researched styles of pagodas in various periods. With his study, he proposed that the stone pagoda style originated in wooden pagodas. This is one of his greatest scholarly accomplishment. Another important contribution is the stylistic classification of Korean Buddhist pagodas. Most of his works were done during 1930s, which was a dark age for Korean people due to the severe colonial oppression of the Japanese. In this period, he demonstrated that Korea has a long and great cultural tradition. He also reminded Korean people of that historically Korean culture was superior to Japanese culture. Go Yuseop stands high in this period along with other nationalist historians for his endeavor to help Koreans recover pride through the knowledge of Korean culture.