포르마린-에탤集卵法에 의한 腸內?蟲類感染의 變化 : Ether Sedimentation Technique

崔東翊,林建 최신의학사 1984 最新醫學 Vol.27 No.12

肝지스도마症의 皮膚反應 及 血淸學的 反應에 對한 硏究 第二報 : 第二編 肝지스도마症의 血淸學的反應

崔東翊 慶北大學校 醫科大學 1961 慶北醫大誌 Vol.3 No.1

In the serological reaction to the parasitic diseases, since Weiberg in 1909 utilized the complement fixation test for the diagnosis of hydatid diseases for the first time. Fairley, Sia, Greval, Sen Gupta, Senekji, Guerreiro, Machado and Kelser have applied the precipitin reaction and the complement fixation reaction for the diagnosis of Schistosomiasis, Leishmaniasis and Chaga's diseases. The recent reprosentative reports on the serological reaction to the parasitic diseases are as follows: Filariasis by Suessenguth, Schistosomiasis japonicum by Wright and Bozicevich, and Paragonimiasis by Yokogawa. As for the serological reaction to the Clonorchiasis, the data on the complement fixation test and the skin reaction are relatively few, and little has also been reported on the hemagglutination and the precipitin reaction of Clonorchiasis so far. Ⅰ. Precipitin reaction. 1) In the sera of the infected and sensitized rabbits, the precipitin reaction appears with protein fraction and polysaccharide fraction. 2) In the sensitizaton of rabbits, the precipitin reaction appears more easily and strongly with protein fraction than with polysacchride fraction. 3) The precipitin reaction in the sensitized rabbits appears within 5 days and the skin reaction does from 20 days to 30 days after the last sensitization in all cases. 4) In the infected rabbits, the skin reaction appears in 30 days and the precipitin reaction does in 40 days. 5) The precipitin reaction and the skin reaction in the sensitized rabbits appears earlier than those in the infected rabbits. 6) In human body, the precipitin reactions appears 85%(68 of 80) in protein frsction, 87.5%(70 of 80) in polysaccharide fraction, and 70.9%(44 of 60) in the saline extracts of adult Clonorchis sinensis. 7) In human body, the precipitin reaction shows better with with polysaccharide fraction than with protein fraction. 8) In Paragonomiasis, the group reaction shows 87.5%(70 of 80) with protein fraction, 95.6%(57 of 60) with polysaccharide fraction, and 91.3%(42 of 45) in the saline extracts of the adult clonorchis sinensis. Ⅱ. Hemagglutination. 1) There elicites no reaction in Clonorchiasis with polysaccharide fraction but shows 86.8%(96 of 128) with protein fraction. 2) In Paragonomiasis, the group reaction shows 73.4%(94 of 128) with protein fraction. 3) The titer of hemagglutination is higher in Paragonomiasis than in Clonorchiasis.

腹腔渗出細胞를 移入받은 마우스와 햄스터에서 肝吸蟲에 대한 免疫應答의 比較

崔東翊,鄭東一,李龍宰 慶北大學校 醫科大學 1987 慶北醫大誌 Vol.28 No.2

肝吸蟲의 代謝産物로 마우스와 golden햄스터를 感作하여 4日 經過한 後 이들 動物에서 腹腔渗出細胞를 採集하여 正常마우스와 햄스터의 腹腔內에 注入한 다음 challenge 感染시켜 肝吸蟲에 對한 BALB/c마우스와 golden햄스터의 免疫應答을 比較하였다. 마우스와 햄스터는 各各 2群으로 나누어 第Ⅰ群 마우스는 adjuvant와 肝吸蟲 代謝産物의 混合物을 腹腔內에 注入하였고 第Ⅱ群 마우스는 同混合物을 footpad에 皮下注射하였으며 第Ⅲ群 햄스터는 同混合物을 腹腔內, 第Ⅳ群 햄스터는 同混合物을 footpad의 皮下에 注射하였다. 4日 經過한 後 마우스와 햄스터를 모두 屠殺하여 腹腔渗出細胞를 採集하였다. recipient마우스는 2群으로 나누어 第1群은 第Ⅰ群 마우스의 腹腔渗出細胞를 第2群에는 第Ⅱ群 마우스의 腹腔渗出細胞를 各各 腹腔內에 注入하였다. recipient 햄스터도 2群으로 나누어 第3群은 第Ⅲ群 햄스터의 腹腔渗出細胞를, 第4群에는 第Ⅳ群햄스터의 腹腔渗出細胞를 腹腔內에 注入하였으며 第5群은 마우스와 햄스터를 對照群으로 두었다. 腹腔渗出細胞를 移入한 4日後에 마우스와 햄스터에 肝吸蟲 被襄幼蟲을 經口的으로 challenge 感染시켜 隔日로 EpG로 計測하였으며 約 40日 後에 모두 屠殺하여 마우스와 햄스터에서의 EpG變動狀, worm burden 및 脾臟當 plague形成細胞數等을 比較하였다. 마우스에서의 EpG는 challenge感染後 23日에 처음 나타났고 29日과 31日에 最大値를 나타내었는데 比하여 햄스터에서는 18日에 처음 나타났고 그後 서서히 增加되었으며 39日에서 43日 사이에 最大値를 나타내었다. 肝吸蟲의 蟲體回收率과 plaque形成細胞數는 마우스와 햄스터 間에 有意的 差異를 認定할 수 없었다. 以上의 成績으로 미루어보아 肝吸蟲의 實驗에는 마우스보다 햄스터가 적절한 것으로 나타났다. This study was performed to compare the role of peritoneal exudate cells between recipient BALB/c mice and golden hamsters. The peritoneal exudate cells obtained from donor mice and golden hamsters were sensitized with the admixture of the metabolic products of Clonorchis sinensis and incomplete adjuvant. The immunologic reactions in mice were compared with those in hamsters. The donor mice and hamsters were divided into 2 groups. Group Ⅰ mice were injected intraperitoneally with the admixture, and Group Ⅱ mice into the footpads. Similarly, Group Ⅲ hamsters were injected intraperitoneally and Group Ⅳ hamsters into the footpads. Four days after sensitization, the mice were killed by neck fracture and the hamsters by deep anesthesia, and then the peritoneal exudate cells were collected from both animals. Recipient mice and hamsters were divided into 2 groups. The mice of Group 1 were injected intraperitoneally with peritoneal exudate cells from Group Ⅰ donor mice and those of Group 2 were injected with the cells from Group Ⅱ mice. At the same time, the hamsters of Group 3 were injected intraperitoneally with peritoneal exudate cells from Group Ⅲ donor hamsters and those of Group 4 were injected with the cells from Group Ⅳ hamsters. The Group 5 mice and hamsters served as controls. Four days after the injection of donor's peritoneal exudate cells, recipient mice were challenged orally with 20, and hamsters with 50 metacercariae, and Eggs per Gram(EpG) of fecal samples were counted from 15 to 43 days after challenge. Mice and hamsters were killed about 40 days after challenge, and the transfer of immunity to the recipients was estimated by significant differences in EpG, mean worm burdens, and immune cells per spleen between experimental and control groups, and between mice and hamsters. The experiments revealed that the EpG of mice appeared for the first time in 23 days, then reached the peak between 29 and 31 days after challenge, whereas, the EpG of hamsters appeared in 18 days and increased progressively, then reached the peak between 39 and 3 days. However, there were no significant differences in the worm burdens and the number of plaque forming cells between mice and hamsters. It is likely in the present study that the golden hamster is a more appropriate animal in the transfer of immunity against C. sinensis than the mouse.

文川池 淡水魚에서의 吸蟲類 被囊幼蟲의 寄生狀

崔東翊,朴鍾大 慶北大學校 醫科大學 1986 慶北醫大誌 Vol.27 No.1

1984年 3月부터 同年 9月까지 慶北 慶山郡 珍良面 文川池에서 淡水魚를 採集하여 吸蟲類 被襄幼蟲의 寄生與否와 그 寄生程度를 調査하였다. 文川池에서 採集한 6種 鯉科魚類에서 6種의 吸蟲類 被襄幼蟲과 所屬未定 被襄幼蟲을 찾아 볼 수 있었다. 肝吸蟲 被襄幼蟲은 3種의 淡水魚, 몰개, 참붕어 납지리에 構川吸蟲 被襄幼蟲은 1種의 淡水魚 붕어에 Cyathocotyle orientalis 幼蟲과 Exorchis oviformis 幼蟲은 5種의 淡水魚에 Metacercaria hasegawai 幼蟲은 2種 Metarchis orientalis 幼蟲은 3種의 淡水魚에 寄生되어 있었다. 魚肉 1 gram 當 肝吸蟲 被襄幼蟲는 몰개에서는 6.9個로 가장 많았고 납지리에서는 0.5個로 가장 적었으며 참붕어에서는 2.6個로 中間値를 나타내었다. 構川吸蟲 被襄幼蟲數는 붕어에서 0.01個로 심히 낮았다. Fresh-water fish caught at the pond Muncheon, Chinyang myun, Kyungsan county were examined for the presence of larval trematodes from March to September, 1984. Six kinds of metacercariae and one kind of undetermined larva were found from six species of fish collected in the pond. In thses, larval trematodes, Clonorchis sinensis metacercaria was found from three species, Gnathopogon atromaculatus, Pseudorasbora parva and Paracheilognathus rhombea, and Metagonimus yokogawai cyst from one species, Carassius carassius. Also, Cyathocotyle orientalis and Exorchis oviformis cysts were found in five, Metacercaria hasegawai in two, and Metorchis orientalis in three species of the fish. The intensity of infestation, expressed in the average number of C. sinensis metacercariae per gram of flesh was high (6.9) in G. atromaculatus, low (0.5) in P. rhombea, and intermediate (2.6) in P. parva. In C. carassius, the average number of M. yokogawai metacercaria was very low (0.01).

南大川 銀魚에서의 橫川吸蟲 被囊幼蟲의 寄生樣相

崔東翊,金英愛 慶北大學校 醫科大學 1986 慶北醫大誌 Vol.27 No.1

慶北 珍面郡 平海面 南大川에서 銀魚를 採集하여 構川吸蟲 被襄幼蟲의 寄生狀을 1984年 6月부터 同年 9月까지 調査하였다. 南大川의 河口와 下流에서 採集한 銀魚에서의 構川吸蟲 被襄幼蟲의 寄生率은 모두 100%였다. 魚體部位別로는 魚肉 100%, 꼬리 94.6%이며 지느러미 86.6%, 鱗片 66.6%의 順位였다. 銀魚 魚肉 1 gram 當 本幼蟲數의 平均値는 238.7個였고, 鱗片 200個當 本幼蟲數의 平均値는 3.0個였다. 構川吸蟲 被襄幼蟲의 月別 寄生狀에 있어서는 6月에 採集한 銀魚에는 全然 寄生되어있지 않았는데 比하여 7月과 9月에 採集한 銀魚에는 모두 寄生되어 있었고 魚肉 1gram當 本幼蟲數의 平均値는 7月 140.6個, 9月 330.5個로써, 7月보다 9月에 더 많았다. From June to September, 1984, sweetfish, Plecoglossus altivelis collected at the delta area and downstream of the river Namdae were examined for the presence of Metagonimus yokogawai cysts. The infestation rate for M. yokogawai was found to be 100 per cent. In detail, the rate was 100 per cent in the flesh, 94.6 per cent in the tail, 86.6 per cent in the fins and 66.6 per cent in the scales, in decreasing order. Intensity of infestation, expressed in the average number of M. yokogawai cysts per gram of flesh was 238.7 and per 200 scales, only 3.0. In the monthly incidence, no M. yokogawai metacercaria was found from the fish examined in June. In contrast, those collected in July and September were heavily infested with the cysts. The average number of metacercariae per gram of flesh was 140.6 in July, and increased to 390.5 in September.

肝吸蟲代謝産物과 蟲體構成物로 感作한 햄스터에서 免疫의 移入

崔東翊,朴武吉 慶北大學校 醫科大學 1987 慶北醫大誌 Vol.28 No.3

肝吸蟲의 代謝産物과 蟲體構成物로 感作한 近交系 golden 햄스터의 腹腔渗出細胞, 脾臟細胞 및 血淸이 recipient햄스터에 免疫을 移入하는지 究明하였다. Donor 햄스터는 5마리씩 2群으로 나누어 第Ⅰ群에는 0.1㎖의 代謝産物과 Freund's incomplete adjuvant의 同量 混合物을, 第Ⅱ群에는 0.1㎖의 蟲體構成物과 adjuvant의 同量 混合物을 ㄹootpad에 2回 注射한 다음 2週後에 麻醉시켜 腹腔渗出細胞, 脾臟細胞 및 血淸을 採集하여 recipient햄스터의 腹腔內에 注入하였다. Recipient햄스터는 5마리씩 6群으로 나누어 第1群에는 第Ⅰ群 donor 햄스터의 腹腔渗出細胞 5×10 exp (5) 및 血淸 1㎖를, 第2群에는 第Ⅰ群의 脾臟細胞 2×10 exp (6) 및 血淸 1㎖를, 第3群에는 第Ⅱ群 donor 햄스터의 腹腔渗出細胞 5×10 exp (5) 및 血淸 1㎖를, 第4群에는 第Ⅱ群의 脾臟細胞 2×10 exp (6) 및 血淸 1㎖를, 第5群에는 非感作對照햄스터의 腹腔渗出細胞 5×10 exp (5) 및 血淸 1㎖를, 第6群에는 對照햄스터의 脾臟細胞 2×10 exp (6) 및 血淸 1㎖를 各各 腹腔內에 注入하여 第1次 感作하였다. 第1次 感作한 7日後 全햄스터에 肝吸蟲 被襄幼蟲 30마리를 經口 challenge感染시켜 15日부터 EpG의 計算을 하였고 challenge 感染後 50日에 모두 屠殺하여 對照群의 worm burden 및 脾臟當 plaque形成細胞數를 基準으로 하여 이들 細胞와 血淸의 注入으로 recipient햄스터에 免疫이 移入되는지 그 與否를 判定하였다. Challenge 感染後 第1, 第5 및 第6群 햄스터에서는 第15日째, 第2, 第3 및 第4群 햄스터에서는 第16日째에 肝吸蟲卵이 formalin-ether 集卵法으로 나타났으며 Eggs per Gram은 Stoll氏 計算法으로 第16日 및 第17日째부터 나타났고, 단도에서 肝吸蟲의 排出로 推定되는 EpG의 急激한 增加는 感作群인 第1, 第2 및 第4群에서는 第28日째, 第3群에서는 31日째에 나타났는데 比하여 非感作對照群인 第5 및 第6群에서는 40日 또는 43日째에 나타났다. 햄스터 단도내 worm burden은 感作群 햄스터(平均 13.2∼15.0마리)는 非感作對照群 햄스터(平均 18.4∼19.0마리)에 比하여 有意的으로 적었다. 脾臟에서 plaque形成細胞는 非感作對照群에서는 檢出할 수 없었고 感作群인 第1, 第2, 第3 및 第4群 햄스터에서는 少數 檢出할 수 있었다. 이 成績으로 미루어 보아 肝吸蟲의 代謝産物과 蟲體構成物로 感作한 햄스터의 腹腔渗出細胞 및 血淸과 脾臟細胞 및 血淸의 recipient햄스터의 腹腔內注入은 免疫이 移入됨을 나타내었다. This study was undertaken to evaluate the role of peritoneal exudate cells(PEC), spleen cells (SC) and serum in the transfer of immunity against Clonorchis sinensis in the isogenic golden hamsters. The donor hamsters (DH) were divided into two groups. One group was sensitized with twice injections of the admixture of the metabolic products of C. sinensis and Freund's incomplete adjuvant into footpads, and the other group was sensitized with the admixture of somatic constituents and the adjuvant. Two weeks after sensitization, the DH were killed by deep anesthesia and the PEC, SC and serum were collected. Recipient hamsters (RH) were divided into 6 groups. The hamsters of Group Ⅰ were injected intraperitoneally (IP) with 5×10 exp (5) PEC and 1 ㎖ of serum from Group Ⅰ DH, those of Group 2 were injected IP with 2×10 exp (6) SC and 1 ㎖ of serum from Group Ⅰ DH, those of Group 3 were injected IP with 5×10 exp (5) PEC and 1㎖ of serum from Group Ⅱ DH and those of Group 4 were injected IP with 2×10 exp (6) SC and 1 ㎖ of serum from Group Ⅱ DH. The hamsters of Group 5 were injected IP with 5×10 exp (5) PEC and 1 ㎖ of serum and those of Group 6 were adminstered IP with 2×10 exp (6) SC and 1 ㎖ of serum from non-sensitized controls. Seven days after primary sensitization, RH were challenged orally with 30 metacercariae and Eggs per Gram (EpG) of fecal samples were counted from 15 to 49 days after challenge. The RH were killed 50 days after challenge, and the transfer of immunity to the RH was estimated by significances in EpG, mean worm burdens, and plaque forming cells per spleen between sensitized and non-sensitized groups. The eggs of C. sinensis appeared in the 15th day after challenge in Groups 1,5,6 and the 16th day in Groups 2,3,4, by the formalin-ether sedimentation and EpG noted in the 16th and the 17th days by Stoll's egg counting techniques. The sudden increase of EpG, which assumed to be expelled the flukes into the intestinal tract, was encountered in the 28 days in Groups 1,2,4, and the 31st day in Group 3. Whereas, it appeared in the 40th day in Group 5 and the 43rd day in Group 6. The mean numbers of sensitized groups (RH of Groups 1,2,3,4) harbored fewer flukes than the non-sensitized groups and the differences in mean worm burdens were significant by the paired t-test. The plaque forming cells were found in the all sensitized groups, but no plaque forming cell was encountered in the nonsensitized groups. It is likely that the intraperitoneal injection of peritoneal exudate cells and serum or spleen cells and serum from the donor hamsters sensitized with the metabolic products and somatic constituents of C. sinensis caused the transfer of immunity to the recipient hamsters.

肝吸蟲 代謝産物과 蟲體構成物로 感作한 마우스에서의 細胞性免疫

崔東翊,殷鍾大 慶北大學校 醫科大學 1985 慶北醫大誌 Vol.26 No.3

肝吸蟲 成蟲의 代謝産物과 蟲體構成物을 Freund incomplete adjuvant와 混合하여 emulsion을 만들어 ICR系 마우스의 footpad에 皮下注射하여 5日, 10日, 15日, 20日 및 30日후에 頸部骨折로 마우스를 잡아 Jerne plaque assay로 마우스 脾臟에서의 plaque 形成細胞의 出現與否와 그 經過를 보았다. 肝吸蟲의 代謝産物과 蟲體構成物로 感作한 마우스의 脾臟에서 plaque 形成細胞를 感作後 5日에 찾아볼 수 있었으며 15日에 그 數가 最大値를 나타내었고 20日 以後부터 적었다. 肝吸蟲 plaque 形成細胞數는 大體로 肝吸蟲의 蟲體構成物보다 代謝産物로 감작한 마우스에서 많았다. 肝吸蟲의 代謝産物과 蟲體構成物로 感作한 마우스에서의 plaque 形成細胞數는 肺吸蟲의 그 數보다 적었다. In order to clarify the cellular immunity of Clonorchis sinensis by the Jerne plaque assay, emulsions were prepared by mixing equal volumes of incomplete Fruend's adjuvant and the metabolites or somatic constituents of Clonorchis sinensis in Medium 199. The mice of highly inbred ICR strain were stimulated by an injection of the emulsions into their footpads. The mice were killed by neck fracture on 5, 10, 15, 20 and 30 days after a primary stimulation. Spleen cell suspensions were obtained from organs of the mice and were used for the determination of plaque forming cells (PFC) by the Jerne plaque assay. PFC were found in the spleen of mice sensitized with the metabolites and somatic constituents of C. sinensis in 5 days after sensitization. The peak of PFC was observed in 15 days and dropped in 20 days after immunization. In general, the mean number of PFC pers pleen of mice sensitized with the metabolites was higher than those with the somatic constituents. Similarly, the numbers of PFC in mice sensitized with C. sinensis were lower than those with Paragonimus westermani.

琴湖江 上流 永川 淡水魚에서의 肝吸蟲 被襄幼蟲의 寄生狀

崔東翊,具玄謨 慶北大學校 醫科大學 1988 慶北醫大誌 Vol.29 No.1

1987年 6月부터 同年 9月까지 慶北 琴湖江流域 上流인 永川에서 投網 및 사발모지等으로 淡水魚를 採集하여 肝吸蟲 被囊幼蟲의 寄生率과 寄生程度를 調査하였고, 그 成績을 西村(1943), Choi(1976), 朴(1978), Hwang 및 Choi(1980)의 成績과 比較하였다. 採集된 淡水魚 12種 중 8種, 즉 참붕어(P. parva), 큰납지리(A. taenianalis), 칼납자루(A. limbata), 몰개(G. atromaculatus), 동사리(O. obscura), 납지리(P. rhombea), 모래모치(P. esocinus) 및 돌고기(P. herzi)等에서 肝吸蟲 被囊幼蟲을 찾아볼 수 있었으며, 그 寄生率은 참붕어(P. parva), 모래모치(P. esocinus) 및 돌고기(P. herzi)에서는 모두 100%였고, 이어서 칼납자루(A. limbata) 65.7%, 납지리(P. rhombea) 61.0%, 몰개(G. atromaculatus) 52.8%, 동사리(O. obscura) 45.5%, 큰납지리(A. taenianalis) 41.4%의 順位로 낮았다. 寄生程度의 標識로서 魚肉 1g當 肝吸蟲 被囊幼蟲의 平均數는 참붕어(P. parva) 87.5個로서 가장 많았고 큰납지리(A. taenianalis)에서 0.7個로 가장 적었으며, 칼납자루(A. limbata), 몰개(G. atromaculatus), 동사리(O. obscura), 납지리(P. rhombea), 모래모치(P. esocinus) 및 돌고기(P. herzi)는 2.7∼33.3個로서 中間値를 나타내었다. 肝吸蟲 被囊幼蟲의 寄生率은 以前의 調査成績보다 大體로 높았으나 그 寄生程度는 魚種別로 若干의 變動을 나타내었다. From June to September, 1987, fresh-water fish were collected by throwing a cast net and using glass bowl containing crushed oil-cake as bait at Yongcheon area in the upper stream of river Kumho, Kyungpook Province, Korea. The infestation pattern of Clonorchis sinensis mesacercaria from the fish was determined and compared with the results reported by Nishimura(1943), Choi(1976), Park(1978) and Hwang and Choi(1980). Twelve kinds of fresh-water fish were collected in the river of Yongcheon, of which, eight kinds of fish, Acanthorhodeus taenianalis, Acheilognathus limbata, Gnathopogon atromaculatus, Odontobutis obscura, Paracheilognathus rhombea, Pseudogobio esocinus, Pseudorasbora parva and Pungtungia herzi were infested with the metacercaria of C. sinensis. The infestation rate for the metacercaria was the highest in P. esocinus, P. parva and P. herzi 100% ; followed by A. limbata 65.7%, P. rhombea 61.0%, G. atromaculatus 52.8%, O. obscura 45.5% and A. taenianalis 41.4%, in decreasing order. In the intensity of infestation with the metacercaria, P. parva was the most heavily infested and the average number of metacercariae per gram of the flesh was 87.5, A. limbata, G. atromaculatus, O. obscura, P. rhombea, P. esocinus and P. herzi were infested intermediately with from 2.7 to 33.3. Whereas, A. taenianalis was infested with the least number 0.7. It was found that the infestation rate for the metacercaria was higher than the results reported by former investigators, however, there was some fluctuation in the intensity of infestation with the cysts.

琴湖江 참붕어에서의 肝吸蟲 被囊幼蟲 寄生狀의 變動

趙元顯,崔東翊 啓明大學校 醫科大學 1984 계명의대학술지 Vol.3 No.2

琴湖江에서 採集되는 참붕어에서 肝吸蟲 被裏幼蟲의 寄生狀의 變化를 究明하기 위해 1983年 3月부터 同年 9月까지 投網과 사발모지로 참붕어를 잡아 肝吸蟲 被裏幼蟲의 寄生率과 그 寄生程度를 調査한 다음 李 및 金(1958), 金(1961), 李(1968), Choi(1976) 및 Hwang 및 Choi(1980)의 調査成積과 比較하였다. 肝吸蟲 被裏幼蟲의 寄生程度의 標識으로서 魚肉 1gram當 被裏幼蟲數는 1958年 63.3個, 1968年 34.3個, 1976年 51.4個, 1980年 41.8個로써 그 數가 起伏을 나타내면서 減少되었으며 이번 1983年 調査에서는 7.9個로 急速히 減少되었음을 나타내었다. In order to determine the recent changes in infestation pattern of Clonorchis sinenisi metacercariae in the southern topmouthed minnow, Pseudorasbora parva, the fish were collected in the river Kumho, throwing a castnet and using a glass bowl containing crushed oil-cake as bait from March to September, 1984. The infestation rates and intensity of infestation for C. sinensis in P. parva were determined and compared with the results reported by Lee and Kim(1958), Kim(1961), Lee(1968), Choi(1976), and Hwang and Choi(1980) in the same river. The intensity of infestation with C. sinensis in P. parva, expressed in the average numbers of cysts per gram of flesh, were decreased with undulation from 1958 to 1980. The average number was 63.3 in 1958, 34.3 in 1968, 51.4 in 1976, and 41.8 in 1980, then dropped abruptly to 7.9 in 1983.

肝吸蟲에서 分離한 蛋白分劃(CPF)의 抗原性에 關한 硏究

崔東翊 경북대학교 의학연구소 1964 慶北醫大誌 Vol.5 No.1

A protein fraction of the adult clonorchis sinen-sis (CPF) was extrated, and its antigenicities were studied by means of intradermal and precipitation reaction on the rabbits and precipitation reaction on the rabbits and human subjects. Rabbits used in this study were divided into three groups; the first group was experimentally infected with metacercaria of Clonorchis sinensis (infected rabbit), the second group sensitized with CPF (sensitized rabbit), the third group served as control (non-infected rabbit). The humen su-bjects was also divided into groups; the first the patient of clonorchiasis, the second the control. Simultaneously, a protein fraction was also extracted from the adult worms of Paragonimus westermani (PPF) and its antigenicity was studied on the human patients of paragonimiasis and controls. Furthermore, the cross reaction of antigenicity between these two different fluk infestation was studied. The results obtained were as follows; 1) In the infected rabbits, the wheal of the intradermal reaction reached the maximum size in 50 minutes and erythema in 60 minutes after the intracutaneus injection of the CPF antigen. 2) In the sensitized rabbit group, wheal and erythema of the intradermal reaction reached maximal size in 60 minutes. 3) In the control group, only wheal was produced following antigen infection, which appeared in 10 minutes and sustained also for 10 minutes, then disappeared in 70 minutes. 4) The intradermal reaction, in the infected rabbits, turned positive on the thirtieth day of experimental infection, reaching maximum size of wheal (5―7㎜.) in 30 days and maintained average diameter of 5 to 6㎜. thereafter. The precipitation began to show positive (1 : 1000 to 1 : 2000) on the fourtieth day of experimental : infection, reaching peak level (1 : 4000) in 100 days, and maintained titer at 1 : 2000 antigen dilution thereafter. The two reactions were parallel in reactivity from the 85 to 100th day after injection. 5) The sensitized rabbits began to show positive precipitation reaction on the fifth day of sensitization, reaction on the fifth day of sensitization, reaching maximum (1 : 16000 to 1 : 32000) in 10 to 20 days, then it decreased rapidly initially and slowly later. The intradermal reaction turned positive on the fifth day; the reactivity increasing while the precipitation decreasing between the 25th and 40th day, and former reaction reached the peak on the 85th to 130th day. 6) In the patients of the clonorchiasis, the positivity of intradermal reaction was more marked than that of precipitation, and no correlation was found between these two reactions. 7) The results of the both reactions by PPF antigen in paragonimiasis was resamble as above 6), but generally, all positivities was higher than above 6). 8) In the results of precipitation and intradermal reaction which were reciprocally tested with serum of clonorchiasis patient by PPF antigen and with serum of paragonimiasis patient by CPF antigen, there were many cross reactions and nospecificity. 9) In human subjects, there was no closs correlation between the intradermal and precipitation reactions, although intradermal reactions were mostly positive in the case of positive precipitations. 10) When both the CPF and PPF antigen were given intracutaneously on the same human patient of clonorchiasis or paragonimiasis, the former antigen prcduced the larger wheal in clonorchiasis, while the latter produced also the larger whcal in paragonimiasis as expected.