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        젖소의 유방염 원인균 분리 및 약제 감수성 검사

        강희정,김익천,김진회,손원근,이두식,Kang, Hee-Jung,Kim, Ik-Chun,Kim, Jin-Hoe,Son, Won-Geun,Lee, Du-Sik 대한수의학회 2001 大韓獸醫學會誌 Vol.41 No.4

        Microorganisms were isolated and identified from bovine 296 quarters which showed positive reaction by California Mastitis Test (CMT) in 40 farms of Jeju from September 1999 to June 2000. The organisms associated with the mastitis of bovine were 11 different bacterial species in this study. Which of them, Staphylococcus aureus was the most predominant species as 152 (51.4%) isolates. Other identified species included 49 (16.5%) coliform, 47 (15.8%) Streptococcus dysgalactiae, 15 (5.1%) Bacillus spp., 8 (2.7%) Staphylococcus epidermidis, 6 (2.1%) Streptococcus agalactiae, 5 (1.7%) Enterococcus faecalis, 5 (1.7%) Corynebacterium spp., 3 (1.0%) Streptococcus uberis, 1 (0.3%) Pseudomonas aeruginosa and 1 (0.3%) Pasteurella haemolytica. Almost of all the islolated beacterial species showed high sensitivity against kanamycin (98.6%), cephalothin (98.0%), streptomycin (94.9%), gentamicin (94.6%), ampicillin (92.2%) and polymyxin B (90.2%). On the contrary, they showed resistance against penicillin (47.0%), tetracycline (37.2%), cefazolin (26.0%), bacitracin (22.6%) and erythromycin (19.9%). Eighty-one isolates were not resistant to any antibiotics and 215 drug resistant isolates showed 89 different drug resistance patterns from single to nine multiple antibiotics resistance patterns.

      • KCI우수등재

        자기회귀모형에서의 로버스트한 모수 추정방법들에 관한 연구

        강희정,김순영,Kang, Hee-Jeong,Kim, Soon-Young 한국데이터정보과학회 2000 한국데이터정보과학회지 Vol.11 No.1

        본 논문에서는 가장 많이 사용되는 시계열 모형중의 하나인 자기회귀모형에서 모수를 추정하는 방법으로 최소 절대 편차 추정법(least absolute deviation estimation)을 포함한 로버스트한 추정방법 (robust estimation)의 사용을 제안하고 모의 실험을 통하여 이러한 방법들을 기존의 최소 제곱 추정 방법과 예측의 관점에서 비교 검토하여 시계열 자료분석에서의 로버스트한 모수 추정 방법의 유효성을 확인해 보고자 한다. In this paper, we study several parameter estimation methods used for autoregressive processes and compare them in view of forecasting. The least square estimation, least absolute deviation estimation, robust estimation are compared through Monte Carlo simulations.

      • KCI등재

        건강보험료 부담의 형평성 변화

        강희정,박은철,이규식,박태규,정우진,김한중,Kang, Hee-Chung,Park, Eun-Cheol,Lee, Kyu-Sik,Park, Tae-Kyu,Chung, Woo-Jin,Kim, Han-Joong 대한예방의학회 2005 예방의학회지 Vol.38 No.1

        Objectives : We analyzed the changes from 1996 to 2002 in distributive equity of the contribution burden in the Korean National Health Insurance. Methods : The study subjects were a total of 8,923 employee households and a total of 7,296 self-employed households over the period from 1996 to 2002. Those were the households meeting the two criteria as completing each annual survey and having no change in the job of head of the household during that period from the raw data of the Household Income and Expenditure Survey annually conducted by the Korean National Statistical Office. The unit of analysis was a household, and this was the standard for assessing the contribution that is now applied on a monthly basis. Deciles Distribution Ratio, Contribution Concentration Curve and Contribution Concentration Index were estimated as the index of inequality. Multiple regression analysis was conducted to compare the annual ability-to-pay elasticity of the contribution to the reference year of 1996 for three groups (all households, the employee households, and the self-employed households). Results : For the index of inequality, the distributive equity of contribution was improved in all three groups. In particular, the employee group experienced a substantial improvement. Using multiple regression analysis, the ability-to-pay elasticity of the contribution in the employee group significantly increased ($\beta$=0.232, p<0.0001) in the year 2002 as compared to the reference year of 1996. The elasticity in the self-employed group also significantly increased ($\beta$=0.186, p<0.05), although its change was smaller than that in the employee group. Conclusions : The employee group had a greater improvement for the distributive equity of the contribution burden than the self-employed group. Within the observation period, there were two important integration reforms: one was the integration of 227 self-employed societies in 1998 and the other was the integration of 139 employee societies in 2000. We expected that the equity of the contribution burden would be improved for the self-employed group since the integration reform of 1998. However, it was not improved for the self-employed group until the year 2000. This result suggests that capturing exactly the beneficiaries' ability-to-pay such as income is the precedent for distributive equity of the contribution burden, although a more sophisticated imposition standard of contribution is needed.

      • KCI등재

        Sphingomonas sp. KS 301의 Superoxide Dismutase 정제 및 특성

        강희정,정재훈,최지혜,손승렬,Kang, Hee-Jeong,Jeong, Jae-Hoon,Choi, Ji-Hye,Son, Seung-Yeol 한국미생물학회 2007 미생물학회지 Vol.43 No.2

        Sphingomonas sp. KS 301, which was isolated from oil contaminated soil, was shown to have five different SODs (SODI, II, III, IV, V) which can be separated by DEAE-Sepharose chromatography, and SOD III was finally purified in this study by ammonium sulfate precipitation, DEAE-Sepharose chromatography, Superose 12 gel filtration and Uno-Q1 ion exchange chromatography. The molecular weight of SOD III was 23 kDa as determined by SDS-PAGE and the apparent molecular weight of the native enzyme was estimated to be approximately 71 kDa by Superose-12 gel filtration chromatography. These data suggest that the purified SOD consists of at least two subunits. The specific activity of the SOD III was higher than Mn type or Fe type SOD of Escherichia coli by 5 fold. To determine the type of SOD III, inhibitory effects of $NaN_{3},\;H_{2}O_{2},\;KCN$ were examined. 10 mM $NaN_{3}$ was able to inhibit 56% of the SOD III activity, which indicates that this SOD is Mn type. The optimum pH of the SOD III was 7.0 and the optimum temperature was $20^{\circ}C$. N-terminal amino acid sequence of purified SOD III was most similar to those of Psudomonase ovalis and Vibrio cholerae among bacteria. 유류오염 토양에서 난분해성 물질인 PAH (polycyclic aromatic hydrocarbon)들을 잘 분해하는 균주 중 SOD (superoxide dismutase) 활성이 높은 균주인 Sphingomonas sp. KS 301의 SOD특성을 알아보기 위하여 Ammonium sulfate 침전, DEAE-Sepharose 크로마토그래피, Superose-12 겔 여과 크로마토그래피, Uno-Q1 이온교환 크로마토그래피를 이용하여 SOD 단백질을 정제하였다. Sphingomonas sp. KS 301은 DEAE-Sepharose 크로마토그래피로 분석한 결과, 기존의 알려진 세균들과는 달리 서로 다른 5가지의 SOD 활성을 가지고 있는 것으로 나타났으며 본 연구에서는 그중 SOD III를 부분 정제하였다. 정제한 SOD III는 Mn type 및 Fe type Escherichia coli SOD와 비교했을 때 비활성도(specific activity)가 5배로 높게 나타났다. SOD III의 분자량은 SDS-PAGE에서는 23 kDa으로 측정되었으며 Superose-12겔 여과 크로마토그래피 후 native 상태의 분자량은 71 kDa으로 정제한 SOD는 3개의 소단위체로 구성되어 있는 것으로 보여진다. 정제한SOD III의 최적 pH는 7.0 이었고 $20^{\circ}C$에서 최적의 활성을 보였다. 또한 SOD의 종류를 알 수 있는 억제물질 $NaN_{3},\;H_{2}O_{2},\;KCN$를 이용한 억제효과를 살펴보았더니 $NaN_{3}$에만 억제되어 Mn type의 SOD임을 알 수 있었다. 또한 이 효소의 아미노 말단의 아미노산 서열은 Psudomonase ovalis 및 Vibrio cholerae의 SOD와 가장 유사하였다.

      • KCI등재
      • KCI등재

        무베어링 로터 허브의 공기역학적 항력 예측

        강희정(Hee Jung Kang) 한국항공우주학회 2012 韓國航空宇宙學會誌 Vol.40 No.8

        본 연구에서는 비정렬 중첩 혼합 격자계를 사용하는 전산유체기법으로 무베어링 로터허브의 공기역학적 항력을 계산하였다. 동체와 로터 허브 모두 점성 항력보다는 압력 항력이 주요 요소로 작용하고 있으며, 토크 튜브의 항력이 허브 항력에서 가장 큰 비중을 차지하고 있음을 확인할 수 있었다. 허브 항력은 동체 항력 대비 39~41%를 차지하는 것으로 나타났다. 최종적으로 개발된 헬리콥터의 항력 추세와의 비교를 통해, 설계된 무베어링로터 허브의 항력은 요구도를 충족시키는 것으로 확인되었다. In this study, aerodynamic drag of a bearingless rotor hub was predicted by computational fluid dynamics methodology using unstructured overset mixed meshes. The calculated results showed that the drag due to pressure forces rather than the viscous drag act as a major factor on both the fuselage and rotor hub, and the drag acting on the torque tube accounted for the largest portion in the hub drag. It was also found the hub drag accounted for 39 ~ 41% of the fuselage drag. Finally, the result confirmed the drag of the designed rotor hub satisfied the requirement of the aerodynamic hub drag by comparing with the drag trend of developed helicopter.

      • KCI등재
      • 3차원 비정렬 격자를 이용한 항공기 형상 주위의 유동해석

        강희정(Hee Jung Kang),권오준(Oh Joon Kwon) 한국전산유체공학회 1996 한국전산유체공학회 학술대회논문집 Vol.1996 No.-

        Inviscid compressible flows over civil transport aircraft configurations are calculated using three-dimensional unstructured tetrahedral meshes. The equations are integrated in time with an explicit multi-stage Runge-Kutta time-stepping algorithm. The flux terms are discretized based on a cell-centered finite-volume formulation with the Roe's flux-difference splitting. The tetrahedral meshes are generated using an advancing-front technique. Validations are made for flows over the ONERA M6 wing and RAE wing-body geometries. Good agreements are obtained between the numerical results and the experiment. The numerical technique is also applied for the K-100 civil transport configuration.

      • KCI등재

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