Biologically Active and C-Amidated HinnavinII-38-Asn Produced from a Trx Fusion Construct in Escherichia coli

강창수,손승렬,방인석 한국미생물학회 2008 The journal of microbiology Vol.46 No.6

The cabbage butterfly (Artogeia rapae) antimicrobial peptide hinnavinII as a member of cecropin family is synthesized as 37 residues in size with an amidated lysine at C-terminus and shows the humoral immune response to a bacterial invasion. In this work, a synthetic gene for hinnavinII-38-Asn (HIN) with an additional amino acid asparagine residue containing amide group at C-terminus was cloned into pET-32a(+) vector to allow expression of HIN as a Trx fusion protein in Escherichia coli strain BL21 (DE3) pLysS. The resulting expression level of the fusion protein Trx-HIN could reach 15~20% of the total cell proteins and more than 70% of the target proteins were in soluble form. The fusion protein could be purified successfully by HiTrap Chelating HP column and a high yield of 15 mg purified fusion protein was obtained from 80 ml E. coli culture. Recombinant HIN was readily obtained by enterokinase cleavage of the fusion protein followed by FPLC chromatography, and 3.18 mg pure active recombinant HIN was obtained from 80 ml culture. The molecular mass of recombinant HIN determined by MALDI-TOF mass spectrometer is 4252.084 Da which matches the theoretical mass (4252.0 Da) of HIN. Comparing the antimicrobial activities of the recombinant hinnavinII with C-amidated terminus to that without an amidated C-terminus, we found that the amide of asparagine at C-terminus of hinnavinII improved its potency on certain microorganism such as E. coli, Enterobacter cloacae, Bacillus megaterium, and Staphylococcus aureus.

膨脹率이 一定한 超音速 노즐흐름에 있어서 非平衡 凝縮이 傾斜衝擊波에 미치는 影響

강창수,권순범,김병지,홍종우 대한기계학회 1990 대한기계학회 춘추학술대회 Vol.- No.-

外筒과 윗板이 回轉하는 二重圓筒內에서의 定常層流 流動解析

姜昌壽,權純範,李鍾鵬 경북대학교 공과대학 1981 工大硏究誌 Vol.10 No.-

In this paper, steady laminar flow in a tank of double cylinder with rotating outer cylinder and upper disc was analyzed numerically. For simplicity, the stream function and vorticity have been introduced into the Navier-Stokes and the continuity equations. The transformed governing equations were converted into the fimite difference equations which were solved numerically by ADI (Alternating direction implicit) and SUR (Successive under relaxation) method.

회전굽힘피로시 크랙의 전파에 관하여

姜昌壽,權永斗 慶北大學校 1977 論文集 Vol.24 No.-

The propagations of crack in rotary bending fatigue test were investigated. The results can be summurized as follows. (1+ / _0)=(1+ _f/ _0)^(Exp[K(N*-1)]) It contains initial crack(or notch) length, final crack length which can be expressed in terms of stress, and experimentally determined constant. The final crack lengthes of smooth or notched specimen are equal near the fatigue limit of the smooth specimen and show a certain linear relationship with the stresses.

國産 鋼板의 成形性에 對한 硏究

姜昌壽,金載昊,崔泰珉 慶北大學校 1978 論文集 Vol.25-26 No.-

To investigate the Plastic-Formability of domestic steel sheets, the sheets were cold-rolled to final thickness and were annealed at various temperatures for various soaking times in a furnace filled with inert-gas. The properties relative to plasticformability, n--values, r--values, CCV and Erichsen values, along with the common mechanical properties are carefully examined for each annealing condition. The optimal conditiconfor plastic-formability are investigated and determined as 600℃-700℃ annealing temperature with 30 min soaking time. Data of all the test results are contained in Appendix for reference.

Coil Spring의 振動傳達 特性에 관한 考察

姜昌壽,金載昊 慶北大學校 1971 論文集 Vol.15 No.-

This study attempts an analytical investigation of coil springs with significant mass under vibratory motion. Equations are presented for both displacement of the free end and transmitted force on support of spring. Equations were solved by applying standard Fourier's techniques and several features of the results are pointed out. It is shown that the common spring may cause an uncommon problems unless more attention is paid to the dynamic characteristics of spring.

JPEG2000 코덱을 위한 최적의 데이터 형식 모델링

강창수,서춘원,Kang, Chang-Soo,Seo, Choon-Weon 대한전자공학회 2005 電子工學會論文誌 IE (Industry electronics) Vol.42 No.4

최근의 통신매체로는 함축적이고 인식이 용이한 영상/비디오가 매우 선호되고 있다. 그러나 이들 데이터는 그 양이 과다해 영상/비디오 데이터의 압축의 큰 연구과제가 되어 왔다. 본 논문에서는 영상압축 시 성능을 크게 좌우할 수 있는 데이터 형식의 최적화에 관련되고, 웨이블릿 변환과 이를 이용한 JPEG2000을 기반으로 한다. 본 논문에서는 이산 웨이블릿 변환 시 이용되는 데이터 형식을 결정하는데 있어서의 기준을 주파수 변환 및 양자화 과정에서의 데이터 손실 양으로 설정하였으며, 이를 바탕으로 실험적으로 최적의 데이터 형식을 도출하였다. 그 결과, 필터계수는 (1, 9)의 10-비트 고정소수점 형식, 웨이블릿 계수는 (9, 7)의 16-비트 고정소수점 형식이 최적임을 확인하였다. Recently, images/videos have been preferred as the communication media because of their information-implication and easy recognizability. But the amount of their data is so large that it has been the major research area to compress their data. This paper is related to optimization in the image data format which can make a great effect in performance of data compression and is based on the wavelet transform and JPEG2000. This paper established a criterion to decide the data format to he used in wavelet transform which is on the bases of the data errors in frequency transform and quantization. This criterion has been used to extract the optimal data format experimentally. The result were (1, 9) of 10-bit fixed-point format for filter coefficients and (9, 7) of 16-bit fixed-point data format for wavelet coefficients and their optimality was confirmed.

多工程加工時 切削條件의 最適化

姜昌壽,金載昊,權永斗,李永文 慶北大學校 産業開發硏究所 1979 硏究報告 Vol.7 No.-

The selection of maching conditions based on minimum production cost and maximum production rate is analyzed for a multi-stage machining model which consists of rough cuttings, finishing cutting and honing. The problems are formulated as standard mathematical programming problems, which subject to several constraints such as cutting velocity, feed rate, required roughness etc., and mixed penalty method is used to solve the problems. The procedure used in this study can be applied to many actural multi-stage machinings.

나노 구조에서 실리콘 산화 절연막의 스트레스 유기 누설전류

강창수 대한전자공학회 2002 電子工學會論文誌 IE (Industry electronics) Vol.39 No.4

In this paper, the stress induced leakage currents of thin silicon oxides is investigated in the ULSI implementation with nano structure transistors. The stress and transient currents associated with the on and off time of applied voltage were used to measure the distribution of high voltage stress induced traps in thin silicon oxide films. The stress and transient currents were due to the charging and discharging of traps generated by high stress voltage in the silicon oxides. The transient current was caused by the tunnel charging and discharging of the stress generated traps nearby two interfaces. The stress induced leakage current will affect data retention in electrically erasable programmable read only memories. The oxide current for the thickness dependence of stress current, transient current, and stress induced leakage currents has been measured in oxides with thicknesses between 113.4${\AA}$ and 814${\AA}$, which have the gate area $10^3cm^2$. The stress induced leakage currents will affect data retention and the stress current, transient current is used to estimate to fundamental limitations on oxide thicknesses. 본 논문에서 얇은 실리콘 산화막의 스트레스 유기 누설전류는 나노 구조를 갖는 트랜지스터의 ULSI 실현을 위하여 조사하였다. 인가전압의 온 오프 시간에 따른 스트레스전류와 전이전류는 실리콘 산화막에 고전압 스트레스 유기 트랩분포를 측정하기 위하여 사용하였다. 스트레스전류와 전이전류는 고스트레스 전압에 의해 발생된 트랩의 충방전과 양계면 가까이에 발생된 트랩의 터널링에 기인한다. 스트레스 유기 누설전류는 전기적으로 기록 및 소거를 실행하는 메모리 소자에서 데이터 유지 능력에 영향이 있음을 알았다. 스트레스전류, 전이전류 그리고 스트레스 유기 누설전류의 두께 의존성에 따른 산화막 전류는 게이트 면적이 10/sup -3/㎠인 113.4Å에서 814Å까지의 산화막 두께를 갖는 소자에서 측정하였다. 스트레스 유기 누설전류, 스트레스전류, 그리고 전이전류는 데이터 유지를 위한 산화막 두께의 한계에 대해 연구 조사하였다.

특별손익의 특성에 관한 실증연구

강창수 한국산업경제학회 2004 산업경제연구 Vol.17 No.4

This study is to reconsider the classification problem of ordinary and extraordinary earning by empirically examining 'extraordinary and transitory' condition of the latter. The view adopted in this paper is that when the two conditions are satisfied simultaneously, the classification does make sense. First, I examined 'extraordinary condition' by checking two earnings' independence. Next, I examined 'transitory condition' in two ways. First, I observed if increase of extraordinary earning make abnormal earings's price multiple lessened. Second, I observed if extraordinary earning has the transitory earning's characteristics which Ohlson(1999) showed theoretically.The results are as follows;First, extraordinary earning was not independent of ordinary one. Specifically, extraordinary gain was negatively correlated with ordinary earning.Second, increase of extraordinary earning share in accounting earning did not lessen abnormal earings's price multiple.Third, extraordinary earning has no persistence and informative relevance in stock pricing.In summary, extraordinary earning on income statement was transitory but not extraordinary which means that classification problem is still alive. 본 연구는 특별손익의 ‘비경상적이고 비반복적’ 인 특성을 실증적으로 조사해 봄으로써 경상손익과의 구분의 문제를 재조명해 보고자 하였다. 특별손익의 정의상 특성인 비경상성과 비반복성(이하 일시성이라 칭함.) 각각을 주 연구대상으로 하여 이러한 두 특성이 동시에 충족되는 경우 경상손익과의 구분문제는 발생하지 않는 것으로 보았다. 먼저 특별손익의 비경상적 특성은 경상손익과의 독립성을 통해 파악해 보고, 다음으로 특별손익의 일시성은 i) 특별손익의 비중의 증가가 초과이익평가모형상 초과이익 주가배수의 감소를 가져오는 지를 통해 그리고 보다 직접적으로 ii) 특별손익이 Ohlson(1999)이 정의한 일시적 손익의 특성에 부합하는 지를 통해 검증해 보았다. 연구결과는 다음과 같다. 첫째 특별손익의 발생은 경상손익의 발생과 독립적이지 않았다. 구체적으로는 특별이익과 경상손익이 유의한 음(-)의 관계를 보였다. 둘째 특별손익의 단순한 비중 증가는 초과이익 주가배수의 감소를 가져오지 않았다. 셋째 특별손익은 자체의 미래 지속성이 없으며 주식가치결정에 대한 정보적 관련성이 없음을 보여주었다. 요컨대 특별손익은 1997년 이후 6년간 그 일시적 특성을 유지하였으나 비경상성은 그렇지 못한 것으로 나타났다. 본 연구는 특별손익의 정의상의 특성인 비경상성 및 일시성을 실증했다는 점과 이를 기초로 자의성으로 인한 두 손익의 구분의 문제가 여전히 있을 수 있음을 부분적으로 확인하였다는 점에 그 의의가 있다 하겠다.