Antithrombotic and Anticoagulant Components of Plants

Han, Yong-Nam 경희대학교 동서의학연구소 1999 INTERNATIONAL SYMPOSIUM ON EAST-WEST MEDICINE Vol.1999 No.1

Yong-Nam Han. Natural Products Research Institute, Seoul National University, Seoul, korea.Antithrombotic and Anticoagulant Components of Plants. Proceedings of International Symposium on East-West Medicine, Seoul. Page 149-152, 1999.-There are many kinds of herbal drugs related to "Blood"(現血藥)in the Oriental Traditional Medicines. In order to investigate the antithrombotic and anticoagulant activities of blood related medicinal plants, we have utilized some screening methods such as intrinsic and extrinisic anticoagulant and antiplatelet activities, in vivo and in vitro. In the first study to screen the antithrombotic activity, we have measured the bleeding time(BT), the whole blood recalcification clotting time(WCT) and the plasma reclaification clotting time(PCT), successively, after orally administering an extract of a herbal medicine to rats. An intrinsic pathway anticoagulant drug should prolong all the three kinds of barometers, an extrinsic pathway anticoagulant one should prolong only BT, and antiplatelet one should prolong both BT and WCT. In the second study to isolate activ principles, we have measured in vitro the inhibitory activities on PCT, TF(tissue factor) and PA(platelet aggregation) for the intrinisic pathway anticoagulant, the extrinsic pathway anticoagulant and the antiplatelet activities, respectively. Recently, we have isolated weveral TF inhibitors(the extrinsic pathway anticoagulant) such as pubescenolic acid (20-epi-pomoli acid) from Ilex pubescens roots (毛冬靑), zivu-glycoside Ⅱ and pomoli acid from Sanguisorba officinalis roots (地楡), and compund I and X from Cinkgo biloba leaves(銀杏藥).Their anticoagulant activities were evaluated in vivo and in vitro on rat brain and lung tissue, respectively. Rat TF was measured by its procoagulant activity using one stage clotting assay method modified by us. Ziyu-glycoside Ⅱ, one of the most potent TF inhibitor was effective in vitro, but not in vivo. Thus, we prepared a derivative, named ZYM201, which strongly inhibitied the rat brain TF activity in vivo, Oral administration of ZYM201 enhanced atherosclerotic index in dietary, streptozo-tocin-induced, and alcoholic hyperlipidemic rats (unpublished data).

만성중이염의 골도청력변화

박선홍,노용훈,이도용,나한조,김용기,최봉남,도남용 朝鮮大學校 附設 醫學硏究所 1996 The Medical Journal of Chosun University Vol.21 No.1

We have usually experienced bone conduction impairment in chronic otitis media, but it is unclear that raised bone conduction threshold is due to the reflection of cochlear dam- age and / or Carhart effect ( an artificial elevation of bone conduction threshold due to conductive defect ) or both. The purpose of this study is to verify the influence of the artificial elevation of the bone conduction threshold in chronic otitis media and to estimate Carhart effect. A total of 336 cases of tympanoplasty with unilateral chronic otitis media without any other causes of sensorineural hearing loss was studied. The average threshold levels of the bone conduction in diseased ears ranged from 18.5dB at 4kHz to 12.1dB at 250Hz The longer duration of the disease, the larger perforation size, the more severe damage of the middle ear structure and the presence of the cholesteatoma made bone conduction threshold increased. The postoperative thresholds of bone conduction were Improved in 36 cases (40.4%) of the 89 cases that underwent operation with type I tympanoplasty technique without electrie drilling. The degree of the maximal improvement was 14.3dB at 2000Hz and the minimum was 8.8dB at 250Hz. but these result did differ from a statistically significant degree. The result suggest that the bone conduction threshold in chronic otitis media is elevated artificially by conductive defect and improved by successful reconstructive surgery.

강원도 화천군 수달(Lutra lutra) 서식지의 식생 구조

서형수 ( Hyung Soo Seo ),신영섭 ( Young Seob Shin ),이경은 ( Kyung Eun Lee ),김윤미 ( Yoon Mi Kim ),전미나 ( Mina Jeon ),남택우 ( Taek Woo Nam ),한성용 ( Sung Yong Han ),정연숙 ( Yeonsook Choung ) 한국하천호수학회 2014 생태와 환경 Vol.47 No.special

In order to determine whether vegetation would be one of the factors for the selection of otter home range, vegetation structure and other potential factors were studied in Hwacheon, Korea. Thirteen sites, otter’s activity found and not found, were investigated in North Han River and connected tributary streams of Hwacheon-gun. Three types of vegetation were classified by cluster analysis, which is short grass, tall grass and shrub type. Vegetation zone of each channel is composed of either one type, or mosaic of tall grass and shrub type. Short grass type is common in Lake Paro and upper North Han-river where water level is highly variable throughout a year. Therefore, annual species such as Persicaria nodosa, Fimbristylis dichotomam and Chenopodium ficifolium are the most dominant. Shrub type is common at the downstream sites of Jichon stream and along mainstream of North Han River down Lake Paro. A shrub species, Salix koreensis, is the most common. Tall grass type is dominant occupying the most vegetation zone of the tributary channels. Phragmites japonica is absolutely dominant. Due to its dense cover, a few plant species are co-existed. Otter activity was found in all three vegetation types and no marked activity was found at some sites of tall grass type. There is no difference in species composition and physiognomy between tall grass sites with and without otter activity, while it shows significant difference in fish availability between two groups. Overall we found that home range of otters in the region is along the mainstream and downstream of tributary streams with high fish availability in all vegetation types and in various human activity levels.

國民學校 兒童들의 運動傷害 調査 : focussed on 4 districts in Kangweon province 江原道內 4個地域을 中心으로

韓相俊,金龍洙,吳壽一,朴南煥,洪寬伊,兪玉在 江原大學校附設 體育科學硏究所 1983 江原大學校附設體育科學硏究所論文集 Vol.- No.8

To suggest the basic data for a precautionary measures against sports injuries and safety educaion in Elementary school boys and girls, 1,149 boys and 1,111 girls(Total 2,260 answerers) who are 4th, 5th, 6th grade of Elementary school residing 4 districts((city, farm, fishing, and mining village) in Gang-weon province were answered on the questionair which is contents, 21 numbers of questions from Oct. 3, 1982. to Nov.3, 1982. The conclusions from gained results by analyzed data are as follows: 1. The most favorite sports Boys, Baseball and soccer Girls: Running and lope jumping Especially, boys residing in city area answered that the most favorite sports is baseball, and boys residing in the other districts answered that it is soccer. 2. The time required for carry out sports. About 85% of tatal answerer carry out sports 30min. to 1 hour per day. 3. Causes of sports injuries. Taking no notice of boys and girls carelessness and insufficiency of practice on the skill is the most. The next order, foul and a dangerous place in boys and fitness difficency in girls. 4. Situation injuries. When fall down a body toward the front was the most. During mining and collision against the other body were next order. 5. Injuried places and the weather. Out-door ground was most, the weather when injuried was clear. On the other hand, over 80% of total answere injuried season were summer and fall. 6. Sports events which caused sports injuries. Boys: Mainly soccer and baseball Girls: Running and lope jumping 7. Injuried part of the body was represented following order: ①The knee ②The ankle ③Hand and the charecteristic special features of injuries were sprain and laceration in addition, its symptom was acute form of decease. 8. treatment after injuried Boys and girls care at their home but 3.9% among total answeres who were injuried, and duration of curing was and day to 30 days. 9. A trend of experience on sports injury. 84% among total answerers in boys, and 75% among total answerers in girls. Thus it shows the experience ratis are more in boys than in girls.

자기통제 피드백이 배구 언드핸드 토스의 수행과 학습에 미치는 영향

한남익,박종태,표내숙,박철용 釜山大學校 附設 體育科學硏究所 1998 體育科學硏究所 論文集 Vol.14 No.-

The purpose of this study was to investigate where a feedback(self-controlled) schedule controlled by learner would be a more effective means of delivering feedback than any predetermined feedback schedule. Subjects(n=60) were randomly assigned to one of five conditions : Control condition, 50% relative frequency feedback condition, bandwidth feedback condition, self-controlled performance feedback condition, and yoked control condition. Experiment was designed to determine the optimal feedback condition for performance and learning of underhand toss. In experiments, subjects performed 50 acquisition and two retention trials blocks were analyzed with two experimental conditions to which they had been assigned. Data obtained from all subjects in five acquisition and two retention trials blocks were analyzed with two separate analyses of variance for each of four error scores. Analysis performed on the acquisition trials were a 5×5(condition ×trial block) analysis of variance(ANOVA) with repeated measures on the second factor. The retention trials were analyzed in a 5 ×2(condition ×trial block) analysis of variance with repeated measures on the second factor. Dependent measures were absolute error(AE), and variable error(VE). In conclusion, it should be stressed that the results of this study do support the guidance hypothesis of feedback and these findings showed that self-controlled subjects tend to produce improvements in the learning of underhand toss.

鷄龍山의 水棲昆蟲 群集에 관한 硏究

남상호,조영호,박영준,한용구,이정수 대전대학교 기초과학연구소 2002 自然科學 Vol.13 No.2

천황봉에서 발원하여 산간 계류를 형성하고 흐르다가 유성천과 합류해 금강으로 유입되는 계룡산 동학사 계곡의 수선곤충 군집을 조사한 결과 총 5목 17과 37종이 출현하였다. 이중에서는 하루살이류가 6과 14종으로 가장 많이 출현하였으며, 현존량은 파리류가 전체 1,165개체중 569개체(48.84%)로 가장 높게 나타났다. 전체 우점종은 꼬마하루살이와 깔다구류로 0.15의 우점도지수를 기록하였다. 종다양도는 제 4지점인 남매탑 계류의 합류점이 0.93으로 가장 높았고, 제 1지점인 은선산장 위 부근이 0.09로 낮게 나타났다. 각 지점별 오수생물계열은 초강부수성-강부수성으로 매우 낮게 조사되었다. Aquatic insect communities were investigated in a mountain streams of YIt. Iiyeryong1 one of the central areas of Korea, from March to September, 2002. As a result, 37 species of aquatic insects in 17 families and in 6 orders appeared during the study period, the order Ephemeroptera and Diptera of which were the most appeared. Ephemeroptera was consisted of 14 species of 6 families, and Diptera was consisted of 589 individuals(48.84%j of total individual nun-her. Dominant species was Baetis ther-micus and the subdominant species was Chironmidae sp.. Dominant species index of Baetis thermicils and Chironmidae sp. was O.15 The highest species diversity index was observed of site 4 as 0.93 and the lowest species diversity index was observed of site 1 as 0.09. Dominance indices ranged between 0.15 and 1.00 while diversity indices between 0.09 and 0.93. In relation to species diversity indices, Mt. Iiyeryong streams were determined as Isosaprobity-Polysaprobity area. In general, it is inferred from the aquatic insect community that typical mountain streams of Korea are low.

pV47-2 다좌위탐식자를 이용한 인체 게놈에서 다형성 유전좌위의 분리

남용석,이혜린,한길로,황적준 大韓法醫學會 1997 대한법의학회지 Vol.21 No.2

Two polymorphic loci, so- called FS106 and FS185, have been isolated from the human genome, using a multilocus probe pV47-2, which is extensively used in Korea for forensic investigation such as resolving paternity disputes. Among the several plaques selected from λ Fix-II genomic libraries, fourteen clones have been characterized. Restriction maps of 14 clones were constructed to define the flanking as well as repeat parts. The repeat-free flanking DNA fragments were tested for single locus specific polymorphism, and repeat containing DNA fragments were sequenced for the design of PCR primers. None of the repeat-free flanking DNA fragments was not shown any polymorphisms by RFLP analysis. The (GGT)??-rich sequences in most of repeat containing DNA fragments were identified by sequencing analysis. Most of repetitive sequences consists of major units of (GGT)??, but a regular repetition pattern can not be found in all clones. Two sets of primers designed from flanking sequences of repeat containing DNA fragments were shown length polymorphisms by PCR analysis, when tested in 50 unrelated individuals. Three and four alleles were detected at FS106 and 0.58 for FS185. In addition, two loci, FS106 and FS185, have been mapped on chromosome 5 and 3, respectively, by somatic cell hybrid analysis.

數種 漢藥材가 肝癌細胞(Hep G2)와 마우스 腹腔 大食細胞에 미치는 影響

康城溶,韓宗鉉,趙南根 대한본초학회 1997 大韓本草學會誌 Vol.12 No.1

The action mechanism of herb drugs on the cytotoxicity and apoptosis in tumors undoubtedly are diverse. Apoptosis often is particularly prominent near foci of confluent necrosis, where mild ischemia is likely to be involved in its initiation. Apoptosis in tumors found in living body is occured by cytokines released from macrophage or cytotoxic T-lymphocyte, but the mechanism is not known accurately. A variety of cancer chemotherapeutic agents have been shown to induce extensive apoptosis in rapidely proliferating normal cell population, lymphoid tissues and tumors. The way in which anti-cancer drugs induce apoptosis is unknown. However, there is an additional important consequence of the realization that anti-cancer drugs mediate their therapeutic affects by triggering apoptosis. The activated macrophage can selectively destroy tumor cells Therefore, macrophage-mediated tumor cytotoxicity can have a important significance. Recently, it has been reported that some crude drugs stimulates IFN-v production in T-lymphocyte, and IFN-v stimulate nitric oxide production in peritoneal macrophage. The nitric oxide can affect the cytotoxicity and apoptosis in tumor cells. In this study, antineoplastic activity against human hepatocellular carcinoma cell line(Hep G2) was tested in four species of herb drugs. The plant materials were extracted with water, and the cytotoxic activity was tested using a calorimetric tetrazolium assay(MTT assay), the apoptosis was tested using a DNA electrophoresis and flow cytometry. The nitric oxide production from mouse peritoneal macrophage was tested using a Griess method. The four species of herb drugs were Arisaemitis Tuber, Bupleuri Radix and Coptidis Rhizoma extracts against the proliferation of Hep G2 cells showed cytotoxicity at the concentration of less than 100ug/㎖, and Bupleuri Radix and Coptidis Rhizoma extracts increased the cytotoxicity of mitomycin C and Coptidis Rhizoma extracts increased the cytotoxicity of cisplatin on Hep G2 cells. Bupleuri Radix and Coptidis Rhizoma extracts against the proliperation of BALB/c 3T3 cells showed cytotoxicity, and Coptidis Rhizoma extracts aginist the proliperation of mouse thymocytes showed cytotoxicity at the concentration of less than 100ug/㎖. Arisaemitis Tuber extracts increased nitric oxide production from macrophage.

PMDA-ODA 폴리이미드의 합성 및 특성에 관한 연구

정용재,한태성,최성부,남석태,김병식 동국대학교 산업기술연구원 1996 산업기술논문집 Vol.8 No.-

Pyromellitic dianhydride(PMDA) 와 Oxydianiline(ODA)를 N, N-dimethylacetamide (DMAc)에 녹여 Polyamic acid(PAA)를 2단계 열이미드화법으로 제조하였다. 제조된 폴리이미드 막의 이미드화도는 FT-IR에 의하여 분석되었다. 또한 PAA의 농도를 10~30wt%로 변화시키며 합성하여 열이미드화 시킨 후 농도에 따른 단면의 상태를 SEM사진을 통하여 관찰하였다. 농도 변화에 따른 밀도와 T_g의 변화는 혼합액체부상법과 DSC로 분석되었다. 열처리 온도를 증가시킬수록 이미드화도는 증가되었고 PAA의 농도가 증가할수록 필름의 단면이 치밀해짐을 알 수 있었다. 또한 제조된 PMDA-ODA 폴리이미드의 T_g는 215℃ 이었다. Polyamic acid(PAA) was synthesised with Pyromellitic dianhydride(PMDA) and Oxydianiline (ODA) in N, N-dimethylacetamide(DMAc). PMDA-ODA polyimide membranes were obtained by two step thermal imidization, and the degree of imidization of polyimide membranes was observed by the FT-IR. After the thermal imidization through the concentration of varied synthesis of PAA, 10~30wt%, SEM photographies of section state were observed. The density and glass transition temperature on the varied concentration were replace with investigated test of density and Differential Scanning Calorimetry (DSC). With increasing thermal treatment temperature, the degree of imidization was increased. While the concentration of PAA increases, the section of membrane was densed. The glass transition temperature was maintained to be constant(215℃) within the PAA concentration range.

표고 폐골목으로 제조한 목질보드의 성질

최용순,황원중,한태형,김남훈,권진헌 江原大學校 森林科學硏究所 1998 Journal of Forest Science Vol.14 No.-

要約표고폐골목의 재활용을 목적으로 폐골목과 정상재의 칩을 일정비율로 혼합하여 얻은 목질보드의 성질을 조사, 검토하였다. 그 결과 폐골목의 목섬유는 정상재보다 다소 짧고 표 면이 거칠었으며 폐골목 변재부의 밀도는 정상재에 비해 상당히 낮았다. 파티클보드의 두께 팽윤율 및 파괴계수의 시험결과 폐골목을 25~50% 정도 혼합한 경우는 정상재와 비슷한 값 을 나타냈다.결론적으로 폐골목은 파티클보드의 재료로 사용될 수 있고 정상재와의 혼합비율은 25~50% 범위에서 조정되어야 할 것으로 생각되었다.ABSTRACTTo provide further information for reutilization of the bed-logs decayed by oak mushroomfLentinus edodes), the properties of wood-based board prepared with mixed materials of normal wood and bed-logs were examined.Wood fibers in bed-logs showed different morphology from normal wood fibers, that is, shorter length and rough surface. Thickness swelling increased with increasing mixing ratio of bed-log. On the other hand, modulus of rupture(MOR) in bending strength decreased. In products, the visual surface of board prepared from higher mixing rate of bed-log chips was smooth.Consquently, it is considered that the optimum mixing rate of bed-log to normal wood is the range of 25-50% and the bed-log can be used for raw material of board instead of normal wood.