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Yi‑Chia Kuan,Venkatesan Thiruvengadam,Jia‑Shin Lin,Jia‑Hsin Liu,Tsan‑Jan Chen,Hsin‑Mao Wu,Wen‑Ching Wang,Liang‑Jwu Chen 한국식물생명공학회 2018 Plant biotechnology reports Vol.12 No.1
The broad-specificity amino acid racemase (Bsar) from Pseudomonas putida catalyzes the racemization of various amino acids, offering a flexible and feasible platform to develop a new non-antibiotic selectable marker system for plant transformation. In the present study, we demonstrated that a Bsar variant, Bsar-R174K, that is useful as a selectable marker gene in Arabidopsis and rice that were susceptible to l-lysine and D-alanine. The introduction of wild-type Bsar, Bsar-R174K or Bsar-R174A into E. coli lysine or asparagine auxotrophs was able to rescue the growth of these microorganisms in minimal media supplemented with selectable amino acid enantiomers. The transformation of Arabidopsis with Bsar or Bsar variants based on d-alanine selection revealed that Bsar-R174K had the greatest efficiency (2.40%), superior to kanamycin selectionbased transformation (1.10%). Whereas, l-lysine-based selection exhibited lower efficiency for Bsar-R174K (0.17%). The progenies of selected Bsar-R174K transgenic Arabidopsis revealed normal growth properties. In addition, Bsar-R174K transgenic rice was obtained on l-lysine medium with an efficiency of 0.9%, and the progenies of the transgenic rice revealed morphologically normal phenotypes comparable with their wild-type counterparts. This study presents the first report of broad range amino acid racemase Bsar-R174K as a non-antibiotic selectable marker system applied in transgenic plants.
Yi-Hsuan Chen,Yi-Hsiang Lai,Ping-Heng Wu,Li-Syuan Chen,Yung-Sen Lin,Chih-Ming Chen 한국공업화학회 2020 Journal of Industrial and Engineering Chemistry Vol.83 No.-
Surface metallization of polymer substrate andfilm adhesion are crucial to the development offlexibleelectronics. This study demonstrates the co-silanization engineering on the polyimide (PI)film by mutualintercropping of two organosilane molecules to improve their grafting orientability and enhance theadsorbability toward the PI substrate and loaded metal atoms. The mutual intercropping-inspired cosilanizationengineering is implemented by using 3-[(trimethylsilyl)ethynyl]pyridine (TEP) as asupporting organosilane to spatially confine the grafting orientation of the supported aminosilane, 3-[2-(2-aminoethylamino)ethylamino]propyl-trimethoxysilane (ETAS), leading to the formation of a wellorientedorganosilane composite nanolayer as adhesion promotion layer forflexible conductors. Theflexible Cu conductor electrolessly deposited on the PIfilm by co-silanization shows improved adhesionstrength (0.9 kgf/cm) compared to those based on mono-silanization (0.51 kgf/cm) and sputtered Ta/Cu(0.4 kgf/cm). Superior deformability (bendability) was also achieved for the co-silanized Cu/PI sample byretaining good electrical property after bending cycle up to 1000.
An integrate information technology model during earthquake dynamics
Chen, Chen-Yuan,Chen, Ying-Hsiu,Yu, Shang-En,Chen, Yi-Wen,Li, Chien-Chung Techno-Press 2012 Structural Engineering and Mechanics, An Int'l Jou Vol.44 No.5
Applying Information Technology (IT) in practical engineering has become one of the most important issues in the past few decades, especially on internal solitary wave, intelligent robot interaction, artificial intelligence, fuzzy Lyapunov, tension leg platform (TLP), consumer and service quality. Other than affecting the traditional teaching mode or increasing the inter-relation with users, IT can also be connected with the current society by collecting the latest information from the internet. It is apparently a fashion-catching-up technology. Therefore, the learning of how to use IT facilities is becoming one of engineers' skills nowadays. In addition to studying how well engineers learn to operate IT facilities and apply them into teaching, how engineers' general capacity of information effects the results of learning IT are also discussed. This research introduces the "Combined TAM and TPB mode," to understand the situation of engineers using IT facilities.
An integrate information technology model during earthquake dynamics
Chen-Yuan Chen,Ying-Hsiu Chen,Shang-En Yu,Yi-Wen Chen,Chien-Chung Li 국제구조공학회 2012 Structural Engineering and Mechanics, An Int'l Jou Vol.44 No.5
Applying Information Technology (IT) in practical engineering has become one of the most important issues in the past few decades, especially on internal solitary wave, intelligent robot interaction, artificial intelligence, fuzzy Lyapunov, tension leg platform (TLP), consumer and service quality. Other than affecting the traditional teaching mode or increasing the inter-relation with users, IT can also be connected with the current society by collecting the latest information from the internet. It is apparently a fashion-catching-up technology. Therefore, the learning of how to use IT facilities is becoming one of engineers’ skills nowadays. In addition to studying how well engineers learn to operate IT facilities and apply them into teaching, how engineers’ general capacity of information effects the results of learning IT are also discussed. This research introduces the “Combined TAM and TPB mode,” to understand the situation of engineers using IT facilities.
陳亭伊(Ting-Yi Chen),謝杏慧(Hsing-Hui Hsieh),林漢明(Han-Ming Lin),陳怡昌(Yi-Chang Chen),黃招憲(Jau-Hsien Huang),郭殷豪(Yin-Hao Kuo),Hsin-Mien Wang 한국외국어대학교 대만연구센터 2016 대만연구 Vol.- No.9
For the promotion of community development, the first step is to investigate the specialty resources and human resources, to understand the process and the current appearance of community development, and to explore the future development vision or direction should be promoted. Secondly, the local smart people must have the abilities of cooperation, communication, cross-domain integration, and linking external support. It is one of the keypoints of adding-value workforce. Therefore, leaders of a community must find the local characteristics of the championship, and have the capacity to promote citizen participation and to integrate external support and cooperation, so that communities can acquire and integrate diverse and sufficient resources in order to succeed in community sustainability. Therefore, the main purpose of this paper is to establish a model of resource inventory and integration. Through the methods of literature analysis and focus group discussion, this paper explores the community cases of Guantu area in Taipei, Shennong Street and Jingliao community in Tainan how to inventory both natural and human resources, to seek community development and value-added directions, to promote civic participation, cooperation, and to link external groups, so as to achieve the success of internal and external resource integration.
Yu-Li Chen,Kung-Liahng Wang,Min-Yu Chen,Mu-Hsien Yu,Chen-Hsuan Wu,Yu-Min Ke,Yi-Jen Chen,Yin-Yi Chang,Keng-Fu Hsu,Ming-Shyen Yen 대한부인종양학회 2013 Journal of Gynecologic Oncology Vol.24 No.1
Objective: To evaluate the clinical outcome and parameters related to coexisting endometrial carcinoma in women with tissuediagnosed endometrial hyperplasia. Methods: Between January 1991 and December 2009, three hundred and eighty-six patients with the presumptive diagnosis of endometrial hyperplasia were retrieved. Among these, one hundred and twenty-five patients were identified as having coexisting endometrial carcinoma in hysterectomy specimens. The three hundred and eighty-six patients were divided into two groups: the hyperplasia-benign group (261 cases) and the hyperplasia-malignant group (125 cases). Several clinical parameters including age, menopausal status, history of abnormal uterine bleeding, obstetrical history, medical history of diabetes and hypertension, BMI, and preoperative pathologic results were investigated. Results: Age ≥53 (odds ratio [OR], 2.40; 95% confidence interval [CI], 1.26 to 4.57), menopausal status (OR, 2.07; 95% CI, 1.14to 3.76), diabetes history (OR, 7.33; 95% CI, 2.79 to 19.26), abnormal uterine bleeding (OR, 3.99; 95% CI, 1.22 to 13.02), atypical endometrial hyperplasia (OR, 7.38; 95% CI, 4.03 to 13.49), and body mass index ≥27 (OR, 3.24; 95% CI, 1.76 to 5.97) were independent risk factors for prediction of endometrial hyperplasia coexisting with endometrial carcinoma. The diagnostic efficacy of atypical endometrial hyperplasia to predict the endometrial hyperplasia coexisting with endometrial carcinoma was better than or similar to those of other independent factors and combinations of these factors. Conclusion: Coexisting malignancy should be considered when examining endometrial hyperplasia patients with the related risk factors, especially atypical endometrial hyperplasia.
The back contact modification in high-efficiency Cu₂ZnSn(S,Se)₄ solar cells by a thin MoO₃ layer
Septia KHOLIMATUSSADIAH,Cheng-Ying CHEN,Wei-Chao CHEN,Yi-Rung LIN,Shao-Hung LU,Meng-Chia HSIEH,Jan-Kai CHANG,Chih-I WU,Ruei-San CHEN,Kuei-Hsien CHEN,Li-Chyong CHEN 한국진공학회 2016 한국진공학회 학술발표회초록집 Vol.2016 No.8