신규 방사성 항암제 DW-166HC 의 소핵시험

문은이(Eun Yi Moon),이진(Jin Lee),이원용(Won Yong Lee),최청하(Chung Ha Choi),이덕근(Dug Keun Lee),유제만(Jei Man Ryu),정용호(Yong Ho Chung),윤성준(Sung June Yoon),박경배(Kyung Bae Park) 한국응용약물학회 1997 Biomolecules & Therapeutics(구 응용약물학회지) Vol.5 No.3

DW-166HC (^(166)Holmium (^(166)Ho)-Chitosan complex) is a new radiopharmaceutic anticancer agent with a broad anti-tumorigenic spectrum, especially against human hepatic cancer. DW-166HC was evaluated for the appearance of micronucleus in polychromatic erythrocytes (PCEs) of mouse bone marrow cells after subcutaneous arid intravenous single administration. Bone marrow cells were prepared at 24 hr and 48 hr after DW-166HC-I (^(165)Ho-Chitosan complex : cold compound) administration and at 24 hr, 72 hr and 2 weeks after DW-166HC (^(166)Ho-Chitosan complex : hot compound) administration. The results showed there was no statistically significant increase of the numbers of PCEs with micronucleus in all DW-166HC-I administered groups compared with a negative control group but there was statistically significant increase of the numbers of PCEs with micronucleus at 24 hr arid 72 hr in all DW-166HC administered groups, which was recovered after 2 weeks from the drug administration. The results also showed the ratio of normochromatic erythrocytes (NCEs) to PCEs of all DW-166HC-I administered groups was not significantly different from that of a negative controi group but there was significant difference of this ratio at 24hr and 72 hr in all DW-166HC administered groups compared with that of negative group, which was also recovered after two weeks from the drug administration. These results suggested that DW-166HC-I may not cause any chromosomal damage but DW-166HC has in vivo mutagenic potential because of its radioactivity.

Protective Effect of Heme Oxygenase-1 on High Glucose-Induced Pancreatic β-Cell Injury

Lee, Eun-Mi,Lee, Young-Eun,Lee, Esder,Ryu, Gyeong Ryul,Ko, Seung-Hyun,Moon, Sung-Dae,Song, Ki-Ho,Ahn, Yu-Bae Korean Diabetes Association 2011 Diabetes and Metabolism Journal Vol.35 No.5

<P><B>Background</B></P><P>Glucose toxicity that is caused by chronic exposure to a high glucose concentration leads to islet dysfunction and induces apoptosis in pancreatic β-cells. Heme oxygenase-1 (HO-1) has been identified as an anti-apoptotic and cytoprotective gene. The purpose of this study is to investigate whether HO-1 up-regulation when using metalloprotophyrin (cobalt protoporphyrin, CoPP) could protect pancreatic β-cells from high glucose-induced apoptosis.</P><P><B>Methods</B></P><P>Reverse transcription-polymerase chain reaction was performed to analyze the CoPP-induced mRNA expression of HO-1. Cell viability of INS-1 cells cultured in the presence of CoPP was examined by acridine orange/propidium iodide staining. The generation of intracellular reactive oxygen species (ROS) was measured using flow cytometry. Glucose stimulated insulin secretion (GSIS) was determined following incubation with CoPP in different glucose concentrations.</P><P><B>Results</B></P><P>CoPP increased HO-1 mRNA expression in both a dose- and time-dependent manner. Overexpression of HO-1 inhibited caspase-3, and the number of dead cells in the presence of CoPP was significantly decreased when exposed to high glucose conditions (HG). CoPP also decreased the generation of intracellular ROS by 50% during 72 hours of culture with HG. However, decreased GSIS was not recovered even in the presence of CoPP.</P><P><B>Conclusion</B></P><P>Our data suggest that CoPP-induced HO-1 up-regulation results in protection from high glucose-induced apoptosis in INS-1 cells; however, glucose stimulated insulin secretion is not restored.</P>

매체순환식 가스연소기 적용을 위한 Ni계 및 NiO계 산소공여입자의 반응특성

류호정,배달희,조성호,진경태 한국화학공학회 2004 Korean Chemical Engineering Research(HWAHAK KONGHA Vol.42 No.1

매체순환식 가스연소기에 적용하기 위한 최적의 산소공여입자를 결정하기 위해 금속산화물(NiO)의 함량 변화에 따라 네 가지 (NiO 26, 44, 58, 78wt%)의 산소공여입자를 제조하여 열중량 분석기에서 환원반응특성을 측정 및 비교하였다. 제조된 입자들 중에서 NiO 약 60wt%인 입자가 산소전달능력 및 반응속도 면에서 다른 입자들에 비해 우수한 성능을 나타내었다. 산소공여입자의 제조과정에서 금속산화물 형태의 영향을 살펴보기 위해 금속산화물의 출발물질로 Ni과 NiO를 사용하여 NiO 함량이 동일한(약 60%) 두 종류의 입자를 제조하였으며 열중량 분석기와 고정층 반응기를 이용하여 반응속도, 재생성, 환원반응 유지시간 및 CO_(2) 선택도 등을 측정 및 고찰하였다. 금속산화물의 출발물질로 NiO를 사용한 입자가 Ni을 사용한 입자에 비해 반복 실험시에 정상반응조건에 빨리 도달하였고 반응속도와 CO_(2) 선택도가 높았으며 환원반응 유지시간인 길게 나타났다. 실험결과에 의해 본 연구에서 고려한 산소공여입자 중에서 금속산화물의 출발물질로 NiO를 사용한 NiO 함량이 60wt%인 입자를 최적의 산소공여입자로 선정하였다. To find a suitable oxygen carrier particle for chemical-looping combustor, four kinds of particles (NiO 26, 44, 58, 78wt%) were prepared and the reactivity of the reduction was measured and investigated by a thermo-gravimetrical analyzer. From the viewpoint of oxygen transfer capacity and reaction rate, the particle containing 60wt% of NiO showed better performance than the other particles. Also, we have prepared two kinds of oxygen carrier particles to investigate the effect of the starting material for a metal oxide component(i.e., Ni based and NiO based particles, where the weight percents of both samples were set at 60wt% as NiO). The reaction rate, regenerative ability, duration of the reduction, and CO₂ selectivity were easured and investigated by a thermo-gravimetrical analyzer and a fixed bed reactor. The results obtained here indicated that the NiO based oxygen carrier particle containing 60wt% of NiO showed better regenerative ability, reaction rate, CO₂ selectivity, and duration of the reduction, compared with the other particles, therefore we selected this particle as an optimum oxygen carrier particle.

頭鍼이 中風患者의 단기간 NIHSS와 MBI에 미치는 영향

류재춘,박인범,김상우,김철홍,서정철,윤현민,장경전,송춘호,안창범 동의대학교 한의학연구소 2002 동의한의연구 Vol.6 No.-

Objective ; The aim of this study was to investigate the effect of Scalp acupuncture on recovery of motor disorders in stroke patients. Methods ; Twenty two patients with poststroke-hemiplegia were randomized into two groups. Ten patients(test group) treated by 2 methods-Scalp acupuncture and body acupuncture. The other twelve patients(control group) treated only by body acupuncture. The activity of daily living was measured with a National Institutes of Health stroke scale(NIHSS) and Modified Barthel Index(MBI). The therapy was performed one a day for 2 weeks. Results ; In terms of score of NIHSS, the test group showed statistically meaningful decrease after 2 week treatment, but the control group showed statistically meaningful decrease after 1 week(p<0.05). And in terms of score of MBI, the test group did not show statistically meaningful increase but the control group showed statistically meaningful increase after 1 week(p<O.05). There was no statistically meaningful difference after 1 and 2 week treatment between the groups. Conclusions ; These results support that test group has almost same effectness compared with control group in improvement of the activity of daily living of poststroke-hemiplegic patients.

근 피판술을 이용한 기관흉막루의 치료

류한영,설정현,김정철,한승세,우상현,최시호 大韓成形外科學會 1991 Archives of Plastic Surgery Vol.18 No.2

Bronchopleural fistula and chronic empyema are the difficult problems in the thoracic surgery field. We experienced 3 cases of chronic empyema and complicated bronchopleural fistulas treated with muscle flap and concomitant thoracoplasty. The causative primary diseases were pulmonary tuberculosis and bronchiectasis. All the three cases have failed to control the chronic empyema and bronchopleural fistula with conventional method such as closed or open drainage and Eloesser flap after lobectomy. The unresponsive infection on bronchopleural fistula and pleural space was well controlled after muscle flap with thoracoplasty. Complete decortication, closure of bronchopleural fistula as much as possible and coverage of closed fistula with good vascularized tissue are the essential factors for the success of operation on chronic empyema and bronchopleural fistula. The results of operations were satisfactory and the respiratory function was well preserved or improved in one patient. But, the contour of chest wall was deformed in patient with extensive concomitant thoracoplasty.

퇴행성 슬관절염 환자 평가 설문도구의 내적 신뢰도, 상관성에 관한 연구

류성룡,안경애,서병관,강중원,이상훈,이재동,최도영,김건식,이두익,이윤호 EAST-WEST MEDICAL RESEARCH INSTITUTE KYUNG HEE UNI 2005 東西醫學硏究所 論文集 Vol.2005 No.-

Objective : The aim of this study is to evaluate internal reliability and correlation among assessment instruments of knee OA. Methods : Data were obtained from 85 patients with knee OA. They were asked to answer VAS pain scale, two disease-specific questionnaires (WOMAC and LFI) and one instrument about health-related quality(KHAQ). Reliability was assessed using cronbach's alpha. Correlation was assessed by examining the pearson's correlation coefficients. Results : Cronbach's alpha of all Index except WOMAC stiffness subscale(.6679) exceeded the value of 0.70. A good correlation was obtained between WOMAC Subscale (especially Pain Subscale) and LFI (.726<cronbach's alpha<.791). Also, Correlation between KHAQ and two disease-specific questionnaires (WOMAC and LFI) was good(.612<cronbach's alpha<.662). Conclusions : All used instruments have good internal reliability. also, There are good correlation among them. The treatment of knee OA must be focused on health related quality. For this, we should use proper assessment instruments.

용존산소농도 예측을 위한 추계학적 다중 입력모형

류병로,한양수,두민호,조성근 大田産業大學校 1998 한밭대학교 논문집 Vol.15 No.1

In the most cases we analyze a single time series without explicitly using information contained in the related time series In many forecasting situations, other events will systematically influence the series to be forecasted(the dependent variables), and therefore, there is need to go beyond a univariate forecasting model. Thus, we must build a forecasting model that incorporates more than one time series and introduces explicitly the dynamic characteristics of the system. Such a model in called a multiple time series model or transfer function model. The purpose of this study is to develop the stochastic stream water quality model of Puyea station in Keum river system. The performance of the multiplicative ARIMA model and the multiple input-single output transfer function model were examined through comparisons between the historical and generated monthly dissolved oxygen series. The result reveal that the multiple input-single output model lead to teh improved accuracy.

법랑아세포종의 임상병리학적 연구

성일용,류성호,신상훈,김욱규,김종렬,정인교,변기정 大韓顎顔面成形再建外科學會 2003 Maxillofacial Plastic Reconstructive Surgery Vol.25 No.1

The authors analyzed retrospectively the 60 patients with ameloblastoma who were treated at Department of Oral and Maxillofacial Surgery, Pusan National University Hospital from MArch, 1990 to December, 1995. The results obtained were as follows: There were 26 males (43.3%) and 34 females (56.7%). The peak age of patients was the third decade (41.7%). The majority of cases occurred in the mandible 93.3%, especially in the angle region (91.1%). Eighteen of 60 patients (30.0%) were associated with an impacted tooth, by impacted 3rd molar teeth (16.6%), molar (10.0%), and premolar and canine (3.4%) in order., Fifteen of 60 patients (25%) were associated with an odontogenic cyst, by dentigenous cyst(13.3%), odontogenic keratocyst(8.4%), and radicular cyst(3.3%) in order. Radiographically, there were 32 unilocular types(54.2%) with average age of 24.9±10.8 years, 14 multilocular types(23.7%) with average age of 35.7±17.7 and 13 mixed types(22.0%) with average age of 41.4±15.8. Histopathologically, there were 42 follicular types(70.0%) with average age of 33.7±15.1 years and 18 plexiform types(30.0%) with average age of 26±14.6. Conservertive treatment was performed in the 33 patients(55.0%) and their average age was 25.2±13.9 years and radical treatment in the 27 patients(45.0%) and their average age was 39.3±41.8. Overall recurrence rate was 10%. A consistent correlation between the age of the patient and the radiographic or histologic type of mandibular ameloblastoma was observed. There was a tendency for ameloblastomas of the follicular type to show a multilocular or mixed appearance and for those of the plexiform type to show a unilocular appearance.

소방구급대원의 응급처치 적절성 평가와 재교육 필요성

류석용,김원율,김경환,이상래,이경호,김홍용,이승한 대한응급의학회 2000 대한응급의학회지 Vol.11 No.2

Background: The prehospital emergency medical system(EMS) for 119 rescue has progressed considerably, but leaves much to be desired. To improve prehospital EMS, we need to evaluate prehospital patient care, and reeducation. Methods: The records of 1,882 patients, who visited Sanggye Paik Hospital Emergency Department via 119 rescue from April 1 to July 30, 1999, were analyzed according to the quality of patient care as documented by the 119 rescue protocol and database. Patients are grouped into 5 classes based on the quality of the care received. Class 1A received adequate care, class 1B received inadequate care, class 1C did not receive the necessary care, and class 2A received unnecessary care, class 2B did not receive unnecessary care. The results of such analyses are presented to the fireman at monthly meetings, thus reeducating them. Results: The overall results were 1027 cases in Class 1A(55%), 83 in 1B(4%), 149 in 1C(8%), 21 in 2A(1%), and 602 in 2B(32%). Well performed care(1A+2B) occured in 1629 cases(87%) and badly performed care(1B+1C+2A) occured in 253cases(13%). Well perfomed care gradually increased from April to July (April 83%, May 85%, June 87%, and July 89%). Conclusion: Meticulous appraisal of the quality of prehospital patient care and reeducation through the regular meetings of EMS physicians and firemen in each community is needed for developing a model protocol for indirect retrospective medical control of the prehospital EMS.

고정화 시스템을 이용한 용균효소의 생산

류병호,박종옥,진성현 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.4

해양에서 분리된 용균활성이 우수한 균주의 균체고정화에 의한 연속생산을 위한 조건을 검토하였다. Bacillus subtilis SH-1의 균체를 고정화하여 column형 reactor에 의하여 연속적으로 효소생산을 실험하였다. 연속발효의 최적조건은 담체는 3.0% sodium alginate로 직경 2.4mm의 bead를 제조하여 기질의 주입속도는 20ml/h 공기의 주입속도는 2.0ml/min이였다. Column형 reactor(용량 2.8ℓ)에 의한 연속발효시 25일까지 76.5~88.0 units/ml의 최적의 효소활성을 갖는 용균효소를 생산할 수 있었다. Bacillus subtilis SH-1 screened from coastal sea water of South Korea was used to produce bacteriolytic enzyme. The production of bacteriolytic enzyme by immobilized cells was investigated. The optimum conditions for the continuous production of the bacteriolytic enzyme using immobilized cells were 2.4 mm diameter of 0.3% alginate beads, 20 ml/h of substrate feeding rate and 20 ℓ/min of aeration rate. A productivity of 76.5 to 88.0 units/ml could be obtained for 25 days by continuous column reactor under the optimum conditions.