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Study on Leaf Production of Angelica acutiloba by Mulching with Polyethylene Film
Choi,Seong-Kyu,Yun,Kyeong-Won,Chon,Sang-Uk,Seo,Young-Nam,Seo,Kyoung-sun,Kwon,Byung-Sun 한국자원식물학회 2003 Plant Resources Vol.6 No.3
This study was conducted to produce effectively more leaves of Angelica acutiloba through year-round production system by using various mulching materials and polyethylene(PE) tunnel. Soil temperature by covering treatment was higher in PE mulching and PE tunnel than in rice straw mulching, especially showing the highest temperature in PE tunnel from April through May. Water content of soil was higher in PE mulching than in non-mulching. Leaf production of Angelica acutiloba through year-round production showed the highest in PE tunnel and root production in PE mulching. These results suggest that leaf production of Angelica acutiloba can be maximized through PE tunnel cultivation system.
A Basic Study on Leaf and Stem Production of Angelica acutiloba
Choi,Seong-Kyu,Yun,Kyeong-won,Chon,Sang-Uk,Lee,Jong-Ill,Seo,Young-Nam,Seo,Kyoung-Sun,Choi,Kyeong-Ju 한국자원식물학회 2004 Plant Resources Vol.7 No.3
This study was conducted to develop effective production system in greenhouse for leaves and stems of Angelica acutiloba by using connect pots in 2003 and 2004. Seed germination rate and plant biomass of Angelica acutiloba collected in 2004 were higher than those harvested in 2003. Germination rate of Angelica acutiloba seeds collected in 2003 was 10%, while germination rate of seeds collected in 2004 was above 90%. Especially, plant growth and yield of Angelica acutiloba grown in connect pots sized with 4×4×5cm(length×width×height) were the highest. These results indicate that leaf and stem production of Angelica acutiloba can be improved by using connect pots and optimizing seed collecting time in greenhouse.
Effect of Activated Charcoal on Growth of Zingiber mioga ROSC
Choi,Seong-Kyu,Yun,Kyeong-Won,Chon,Sang-Uk,Seo,Young-Nam,Seo,Kyoung-Sun,Kwon,Byung-Sun 한국자원식물학회 2004 Plant Resources Vol.7 No.2
Activate charcoal (AC) can be utilized as a soil conditioner in agricultural crop areas. This study was conducted to investigate the effect of AC on growth and yield of Zingiber mioga ROSC as affected by different amounts of AC. The results obtained are summarized as follows. Growth characteristics including plant height and leaf length were the highest when activated charcoal added with 10-30%, suggesting that optimum amount of activated charcoal was ranged from 10 to 30%. Growth and enlargement of the root were improved by 10% AC with higher rhizome length and weight.
재조합효모를 이용한 배양 중 pH변화에 따른 인간 라이소자임의 대량생산
최선욱,이승철,황용일 慶南大學校 附設 基礎科學硏究所 1998 硏究論文集 Vol.12 No.-
Human lysozyme(HLT ; EC 3.4.1.17) is a cell wall lytic enzyme which consists of 130 amino acid residues. We have already prepared an artificial HLY gene from chemically synthesized 38-oligomer with high codon usage in S. cerevisiae. For directing the synthesis and secretion of HLY in S. cerevisiae, a YEp 2-㎛ circle-based vector, pHK501 was constructed by inserting the HLY gene. First, for HLY production during recombinant cell fermentations, compositions of culture mediums, pH, and glucose concentration were optimized. The various complex media containing yeast extract were used to increase the cell mass and HLY activity, but were not recommendable because of low plasmid stability during fermentations. Higher extracellular HLY activity was obtained in the synthetic medium containing 4% initial glucose. Higher HLY activity was observed when pH was controlled to 5.5 in comparison with pH-uncontrolled fermentations. Second, for enhancing the secretion of HLY in recombinant S. cerevisiae, a pH shift process was examined. After reaching stationary growth phase, extracellular HLY activity was significantly increased when pH in culture broth was purposely dropped to 3.0. This pH shift process seems to be very effective strategy for secretion of HLY in periplasmic space.
( Sun Uk Choi ),( Yong Il Hwang ),( Bo Youn Jang ) 한국응용생명화학회(구 한국농화학회) 2011 Applied Biological Chemistry (Appl Biol Chem) Vol.54 No.1
To investigate the effects of PimM and PimR on the increase of natamycin production, exconjugants highly expressing pimM and pimR were constructed. Transcript amounts of pimM and pimR from the exconjugants for their over-expression were 2-fold higher than those of the control strain. Natamycin production of exconjugant for pimM also showed a 2.4-fold increase in liquid culture; however, no effect of pimR was observed. Therefore, promotion of pimM transcription only increases natamycin production.
Kim, Sun-Uk,Park, Young-Ho,Kim, Jin-Man,Sun, Hu-Nan,Song, In-Sung,Huang, Song Mei,Lee, Sang-Hee,Chae, Jung-Il,Hong, Su,Sik Choi, Sung,Choi, Seung-Cheol,Lee, Tae-Hoon,Kang, Sang Won,Rhee, Sue Goo,Chang AlphaMed Press 2014 Stem Cells Vol.32 No.4
<P>Redox balance has been suggested as an important determinant of 'stemness' in embryonic stem cells (ESCs). In this study, we demonstrate that peroxiredoxin (Prx) plays a pivotal role in maintenance of ESC stemness during neurogenesis through suppression of reactive oxygen species (ROS)-sensitive signaling. During neurogenesis, Prx I and Oct4 are expressed in a mutually dependent manner and their expression is abruptly downregulated by an excess of ROS. Thus, in Prx I(-/-) or Prx II(-/-) ESCs, rapid loss of stemness can occur due to spontaneous ROS overload, leading to their active commitment into neurons; however, stemness is restored by the addition of an antioxidant or an inhibitor of c-Jun N-terminal kinase (JNK). In addition, Prx I and Prx II appear to have a tight association with the mechanism underlying the protection of ESC stemness in developing teratomas. These results suggest that Prx functions as a protector of ESC stemness by opposing ROS/JNK cascades during neurogenesis. Therefore, our findings have important implications for understanding of maintenance of ESC stemness through involvement of antioxidant enzymes and may lead to development of an alternative stem cell-based therapeutic strategy for production of high-quality neurons in large quantity.</P>
Jin-Uk Choi,Nak-Jun Choi,홍석경,Tae-Hyun Kim,Min Ae Keum,Se Ra Kim,Sun Ju Lee,Sung-Dae Shin 대한외상중환자외과학회 2018 Journal of Acute Care Surgery Vol.8 No.2
Purpose: Patients in the intensive care unit (ICU) are more susceptible to nosocomial infections, including central line-associated bloodstream infection (CLABSI), surgical site infection, urinary tract infection or ventilator-associated pneumonia. This study is a comparative analysis of how central venous catheter (CVC) management staff affects CLABSI.Methods: We performed a two-phase review of all patients transferred to the surgical ICU (SICU) from January 2013 to June 2014. CVC management staff was introduced in October 2013. Electronic medical records provided the data for a comparative analysis of incidence rates and risks of CLABSI, as well as the subjects’ general characteristics.Results: This study included 248 patients before the introduction of a CVC management staff member and 196 patients after the introduction. General patient characteristics before and after the CVC management staff was in place did not differ significantly. The CLABSI rate decreased by 4.61 cases/1,000 device days after the introduction (6.26 vs. 1.65; odds ratio, 4.47; 95% confidence interval, 1.39∼14.37; p=0.009). However, the mortality rate and length of ICU stay did not change after CVC management staff was in place (12.9% vs. 10.7%, p=0.480; 16.00±24.89 vs. 15.87±18.80, p=0.954; respectively).Conclusion: In this study, the introduction of CVC management staff effectively reduced CLABSI rates in current ICU system.