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심호기,백반석,황성원,배만종 경산대학교 생명자원개발연구소 1996 생명자원과 산업 Vol.1 No.-
감초에서 분리한 각각의 추출물(HWE, ME, AE)을 BALB/c mouse에 투여하여 탐식작용, 용혈반형성, 용혈소 역가특정 및 Rosette형성 실험을 통해 감초가 면역응답에 미치는 영향을 조사하였다. 1. 탐식능 측정에서 복강침출세포(PEC)와 비장세포(spleen cell)는 CON군에 비하여 가각의 약물투여군이 Candida parapsilosis에 대한 효과가 약간 높은 경향을 나타냈지만 유의성은 없었다. 2. 용혈반 형성 및 용혈소 역가측정에서는 CON군에 비하여 HWE, ME, AE투여 군이 유의성을 나타냈다. 3. Rosette형성에 대한 측정에서는 CON군에 비하여 ME, AE투여 군이 높은 유의성을 나타냈다. 본 실험 결과에서 감초 추출물들은 Candida paraprapsilosis에 대하여 약간의 탐식증진효과를 나타냈으며, 항체생성세포의 용혈반 시험 및 용혈소 역가측정과 Rosette형성측정에서의 유의성을 나타낸 것은 감초가 세포성 면역을 주도하는 T세포를 활성화시킬 뿐만 아니라, 체액성 면역을 주도하는 B세포를 활성화시킨다는 것을 확인 할 수 있다. The inverstigation of effecting immune response on BALB/c mouse was experimented with hot water extract(HWE), 50% methanol extract(ME) and aaceton extract(AE) 〔which was extracted from Glycyrrhiza glabra〕. The experiment was carried out by phagocytosis, hemolytic plaque assay, hemolysin titration and rosette assay. Peritoneal exudative cells and spleen cells in phagocytosis ability; the experimental group has shown a slight increase in Candida parapsilosis(HWE, ME & AE). The experiment shows no relevant correlation. The experiment of hemolytic plaque assay and hemolysin titration was treated by HWE, ME and AE. The experimental group was treated by ME and AE through the measurement of rosette assay. The experimental group has given significant increase compared with the control. As the result, the extracts of Glycyrrhiza glabra (HWE, ME and AE) has effedted slightly to Cnadida parapsilosis in phagocytosis. The reason of effecting the immunocyte in hemolysin titration, rosette assay and hemolytic plaque assay ; Glycyrrhiza glabra has activated the T-cell which controlled cell mediated immunity. Same with B-cell which could also control humoral immunity.
Differential Proteomic Analysis of Secreted Proteins from Cutinase-producing Bacillus sp. SB-007
Ban, Yeon-Hee,Jeon, Mi-Ri,Yoon, Ji-Hee,Park, Jae-Min,Um, Hyun-Ju,Kim, Dae-Soon,Jung, Seung-Ki,Kim, Keun-Young,Lee, Jee-Won,Min, Ji-Ho,Kim, Yang-Hoon The Korean Society of Plant Pathology 2008 Plant Pathology Journal Vol.24 No.2
Bacillus sp. SB-007 was isolated from pea leaves harvested from the southwestern parts of South Korea through screening on a minimal medium containing 0.2% purified cutin for its ability to induce the cutinase production. However, no cutinase was produced when it was grown in a minimal medium containing 0.2% glucose. A proteomic approach was applied to separate and characterize these differentially secreted proteins. The expression level of 83 extracellular proteins of the cutinase-producing Bacillus sp. strain SB-007 incubated in a cutinase-induced medium increased significantly as compared with that cultured in a non cutinase-induced medium containing glucose. The extracellular proteome of Bacillus sp. SB-007 includes proteins from different functional classes, such as enzymes for the degradation of various macromolecules, proteins involved in energy metabolism, sporulation, transport/binding proteins and lipoproteins, stress inducible proteins, several cellular molecule biosynthetic pathways and catabolism, and some proteins with an as yet unknown function. In addition, the two protein spots showed little similarities with the known lipolytic enzymes in the database. These secreted proteome analysis results are expected to be useful in improving the Bacillus strains for the production of industrial cutinases.
ATP Alters the Diffusion Mechanics of MutS on Mismatched DNA
Cho, Won-Ki,Jeong, Cherlhyun,Kim, Daehyung,Chang, Minhyeok,Song, Kyung-Mi,Hanne, Jeungphill,Ban, Changill,Fishel, Richard,Lee, Jong-Bong Elsevier 2012 Structure Vol.20 No.7
<P><B>Summary</B></P><P>The mismatch repair (MMR) initiation protein MutS forms at least two types of sliding clamps on DNA: a transient mismatch searching clamp (∼1 s) and an unusually stable (∼600 s) ATP-bound clamp that recruits downstream MMR components. Remarkably, direct visualization of single MutS particles on mismatched DNA has not been reported. We have combined real-time particle tracking with fluorescence resonance energy transfer (FRET) to image MutS diffusion dynamics on DNA containing a single mismatch. We show searching MutS rotates during diffusion independent of ionic strength or flow rate, suggesting continuous contact with the DNA backbone. In contrast, ATP-bound MutS clamps that are visually and successively released from the mismatch spin freely around the DNA, and their diffusion is affected by ionic strength and flow rate. These observations show that ATP binding alters the MutS diffusion mechanics on DNA, which has a number of implications for the mechanism of MMR.</P> <P><B>Graphical Abstract</B></P><P><ce:figure id='dfig1'></ce:figure></P><P><B>Highlights</B></P><P>► The development and use of smFlow-FRET and smPolarization-TIRF microscopy ► Lesion-searching MutS rotationally diffuses in continuous contact with the DNA ► ATP-bound MutS spins freely and in discontinuous contact with the DNA backbone ► Direct visualization of multiple ATP-bound MutS clamps that diffuse along the DNA</P>
Estimation of CH₄ oxidation efficiency in an interim Landfill cover soil using CO₂/CH₄ ratios
Jin Kyu Park,Won Jae Lee,Jong Ki Ban,Eun Cheol Kim,Nam Hoon Lee 대한환경공학회 2015 Environmental Engineering Research Vol.20 No.2
The first objective of this study was to discuss the applicability of the CO₂/CH₄ ratio method in order to assess CH₄ oxidation efficiency. To achieve this objective, a comparison between CO₂/CH₄ ratios and the mass balance method was conducted. The second objective of this study was to estimate the CH₄ oxidation efficiency in an interim landfill soil cover and assess how a CH₄ influx influences the CH₄ oxidation efficiency. The results showed that despite the CO₂ problems brought by respiration, the CH₄ oxidation efficiencies obtained by the CO₂/CH₄ ratio method led to similar results compared to the mass balance method. In this respect, the CO₂/CH₄ ratio method can be an indicator of the CH₄ oxidation efficiencies for landfill cover soils. The CH₄ oxidation efficiencies derived in this study through the CO₂/CH₄ ratio method ranged between 46% and 64%, and between 41% and 62% through the mass balance method. The results imply that the Intergovernmental Panel on Climate Change``s (IPCC) default value of 10% for the CH₄ oxidation efficiency is an underestimation for landfill cover soils. CH₄ oxidation efficiency tends to be negatively correlated with CH₄ influx. Therefore, CH₄ influx reaching a landfill cover should be limited in order to increase the CH₄ oxidation efficiency.
Ginsenoside Rc and Re Stimulate c-Fos Expression in MCF-7 Human Breast Carcinoma Cells
Lee, Young-Joo,Jin, Young-Ran,Lim, Won-Chung,Ji, Sang-Mi,Cho, Jung-Yoon,Ban, Jae-Jun,Lee, Seung-Ki The Pharmaceutical Society of Korea 2003 Archives of Pharmacal Research Vol.26 No.1
We have found that ginsenoside Rc and Re induce c-fos in MCF-7 human breast carcinoma cells at both the mRNA and protein levels. However, neither ginsenoside activated the expression of reporter gene under the control of AP-1/TPA response elements. We have also examined the possibility that ginsenoside Rc and Re act by binding to intracellular steroid hormone receptors that act as transcriptional factors in the nucleus in inducing c-fos mRNA in MCF7 human breast carcinoma cells. However, ginsenoside Rc and Re did not bind to glucocorticoid, androgen, estrogen, or retinoic acid receptors as examined by the transcription activation of the luciferase reporter genes in CV-1 cells that were transiently transfected with the corresponding steroid hormone receptors and hormone responsive luciferase reporter plasmids. These data demonstrate that ginsenoside Rc and Re act via other transcription factors and not via estrogen receptor in c-Fos expression.