소 수정란의 공배양 및 동결이 체외발생에 미치는 영향에 관한 연구

이종진,이명헌,남윤이,이만휘,이봉구,김상근 충남대학교 수의과대학 동물의과학연구소 1996 動物醫科學硏究誌 Vol.4 No.-

The studies on the carried out to investigate the effects of co-culture with cumulus cells and oviduct epithelial cells on the in vitro fertilization and cleavage rate of bovine follicular oocytes, and to determine the optimum thawing temperature and equilibration time of frozen bovine embryos and on survival rate and in vitro developmental rate of bovine embryos. The ovaries were obtained from slaughtered Korean native cows. The follicular oocytes were cultured in TCM-199 medium containing 10 IU/㎖의 PMSG(Sigma, USA), 10 IU/㎖의 hCG, 1 ㎍/㎖의 β-estradiol(Sigma, USA) and 10% FCS(Sigma, USA) for 24-48 hrs in incubator with 5% CO_2 in air at 38.5℃ and then matured oocytes were again cultured for 12-18 hrs with motile capacitated sperm by preincubation of heparin. The bovine embryos following dehydration by cryoprotective agents and a various concentrations of sucrose were directly plunged into liquid nitrogen and thawed in 30℃ water. The results are summarized as followes : 1. The in vitro fertilization and in vitro developmental rates of bovine oocytes co-cultured with cumulus cells in TCM-199 media were 75.0%-76.8% and 17.3%-27.6%, respectively. And in-vitro fertilization rates of cumulus-enclosed oocytes(55.4%) were significantly(p<0.05) higher than cumulus-denuded oocytes(23.1%). 2. The in vitro fertilization and in vitro developmental rates of bovine oocytes co-cultured with 1 ×10^4 cells/㎖, 1 ×10^6 cells/㎖, 1 ×10^8 cells/㎖ and 1 ×10^15 cells/㎖ oviduct epithelial cells in TCM-199 media were 74.5%-77.8% and 15.7%-21.2%, respectively. 3. The in-vitro fertilization and in vitro developmental rates of bovine oocytes co-cultured in TCM-199 media containing PMSG, hCG, PMSG + hCG, PMSG + β-estradiol, hCG + β-estradiol 0 to 40 hrs after insemination were 74.0%-77.4% and 18.9% - 23.1, respectively. 4. The survival rates of bovine embryos thawed after rapid freezing in the media containing various kinds of cryoprotective agents added 0.25M and 0.50M sucrose were 14.7% - 35.1% and 17.6% - 31.6%, respectively. The survival rates of bovine embryos thawed after rapid freezing in the freezing media containing a various concentration of sucrose added 1.5M and 2.0M glycerol, 1.5M and 2.0M DMSO, and 1.5M and 2.0M propanediol were 23.5% -31.4% and 20.6% - 34.1%, respectively. 5. The temperature thawed at 30℃ after rapid freezing of bovine embryos resulted in a significantly higher embryos survival rate than did at 20℃ and 35℃. 6. The equilibration time on the survival rates of bovine embryos was attained after short period of time(2.5-5 min.) in the freezing medium higher than ling period of time(10-20min.).

1년간의 자발적인 운동참여가 중년 남성 제2형 당뇨병 환자의 혈중 지질 성분에 미치는 영향

허만동(Man Dong Huh),이진훈(Jin Hoon Lee),류승필(Sung Pil Ryu) 한국발육발달학회 2006 한국발육발달학회지 Vol.14 No.3

The purpose of this study is to investigate the effects of voluntary exercise participation on blood lipids in type II diabetes mellitus middle-aged men. Eighteen male volunteers were recruited and exercised, jogging, cycle ergometer, swimming, in the sports center for 12 months without any supervision. They had educated heart rate calculation method to keep their target heart rate during exercise. Before and after the training, their body composition, maximal oxygen uptake, and blood lipids profiles were analyzed. Body weight was not changed during experimental periods; however body fat ratio was statistically decreased at the same periods. Blood glucose, TC, LDL-C, and TG were decreased significantly and HDL-C was significantly increased (p<.05). RQ was significantly lower compared to the before exercise and their maximal oxygen consumption and exercise time to fatigue were increased significantly (p<.05). These results suggested that voluntary exercise with simple exercise prescription was also effectual to enhance blood lipids in type II diabetes mellitus middle-aged men.

낭성법랑모세포종, 함치성낭, 치성각화낭의 방사선소견과 Ki-67, PCNA, Cytokeratin 발현과의 연관성에 관한 연구

송만용,이삼선,이진구,이원진,허민석,이재일,민병무,최순철 대한구강악안면방사선학회 2004 Imaging Science in Dentistry Vol.34 No.2

Purpose : To compare the proliferation potential of the epithelial cells between unicystic ameloblastoma (UA), dentigerous cyst (DC), and odontogenic keratocyst (OKC) and to correlate this proliferation potential with the radiographic features of these three pathoses. Materials and Methods : Immunohistochemical expression of PCNA, Ki-67, and cytokeratin as a proliferation marker were assessed for 15 cases of UA, 15 cases of DC, and 15 cases of OKC. The degree of immunochemical expression of three proliferation markers were correlated with the radiographic features, especially cortical expansion (negative and positive) and shape of border (scalloped and round). Results : Using PCNA and Ki-67, OKC showed the highest proliferation potential and UA the lowest. Statistically significant differences were found between the OKC and the UA (p.0.05). However, no statistically significant difference was present according to the radiographic features in all pathoses. Using cytokeratin, there was no significant differences of proliferation potential among three pathoses. Conclusions : OKC epithelium has the most intense proliferation potential, followed by the dentigeous cyst and then unicystic ameloblastoma. There is no significant relation between the radiographic features and the proliferation potential of epithelium of these three pathoses.

건진센터 수검자 자료를 이용한 비만지표에 대한 연구

김상만,최희정,이득주,김광민,윤수진 대한비만학회 1997 The Korean journal of obesity Vol.6 No.2

We have examined l5145 persons(7468 men 7677 women) to determine the role of Obesity Index(body mass index, WHR, body fat percent) on the risk of various chronic disease. All the subjects were pooled from Health Examination Center who took the annual health examination from June, 1994 through Jan, 1997, BMI(Body mass index, kg/㎡), WHR(Waist hip ratio) were measured and correlated with the frequency of chronic disease. In order to evaluated the BMI, WHR, Body Fat as an independent factors on rnorbidity, analysis of covariance was applied adjusted for age. The risk of variows chronic diseases were also analyzed with obesity, defined by BMI above 26kg/㎡ (men), 25kg/㎡ (women), by logistic regression adjusted age and sex. Risk of hypertension was 2.6 with 95% eonfidence interval of 1.9-2.8 in obese group. Risk of diabetes mel1itus, hypercholesterolemia, hypertriglyceridemia, gall stone, fatty liver were 3.2(2.3-4.4), 1.2(0.9-1.4), 2.1(1.7-2.8), 1.4(1.1-2.6) and 1.3(0.8-2.3) respectiveiy. The risk of various chronic diseases were also analyzed with obesity, WHR above 0.9(men), 0.81(women), by logistic regression adjusted age and sex. Risk of hypertension was 1.7 with 95% confidence interval of 1.4 -2.1 in obese group. Risk of diabetes mellitus, hypercholesterolemia, hypertriglyceridemia, gall stone, fatty liver were 3.1(2.3-4.0), 1.2(0.9-l.4), 1.4(1.1-2.6), 2.4(l.8-4.8) and 2.1(1.2-4.1) respectively. The BMI, WHR were independent risk factors for various chronic diseases.

效率的인 下水處理 改善方案에 關한 硏究

이진희,류제천,김송만,박춘수 忠南大學校 産業技術硏究所 1997 산업기술연구논문집 Vol.12 No.2

Greeting the local self-government age, local finance independece rate of local autonomy is on very poor state. So, the method of discharge a cleanest water with the least expense in sewage treatment is an important factor to supply clean water to Chung-Nam, Chung-Buk and Daejoen and to preserve a water quality of Daechong Lake. Therefore, in this study, we analysed not only a facilities outline of environment office and operating state for improvement of a water quality but also investment cost, maintenance cost, reduction method of working expenses for reduction of a budget. As a result of study, it is concluded to improve sewage pipe line of separate type and to install a flow control tank and to introduce a remove technique of T-N for the purpose of water quality improvement. Also, the relation of F/M ratio and outflow quality does not exist a regular proportional expression. But, water is the cleanest condition when the F/M ratio is 0.04. And, the most suitable injection rate of chemical material was 20㎎/ℓ for Al₂(SO₄)₃(8%) and 50㎎/ℓ(0.1%) for Polymer(0.1%). As a reduction method of operation cost, it is necessary to manage a technique support team and manage a foul water plant under township by environment office. As a result of comparison of an electric fee, a chemical cost, a by-produce recycle, and a facilities improvement, total expenses was reduced about 9% in 1996.

N-2-(6-치환-벤조티아졸일)-N'-치환-우레아류의 합성과 정량적 구조-항균력 상관관계에 대한 연구

李萬佶,閔庚鎭 嶺南大學校附設 基礎科學硏究所 1984 基礎科學硏究 Vol.4 No.-

6-Substituted-2-aminobenzothiazoles were prepared from p-Substitutedaniline through the method of Randvere rearrangement. The benzothiazoles were reacted with corresponding isocyanate and 21 of N-2-(6-Substituted(R₁) benzothiazolyl)-N'-substituted (R₂)ureas were synthesized, where R₁is H, methyl, ethyl, propyl and butyl, R₂is methyl, ethyl, propyl or phenyl, The structures of the compounds were determined by means of IR, NMR spectroscopy in addition to elemental analyses. Each compound was tested for antimicrobial activity in vitro on staphylococcus aureus ATCC 25923, Eschrichia coli ATCC 25922 and Cryptococcus neoformans. The results of QSAR of antibacterial activities against staphylococcus aureus indicate that lipophilicity and steric effect of substituent R₁influence on relatively potent inhibition to the growth of S. aureus.

식용 버섯과 약용버섯의 기능성 식품으로서의 전망

이진만,이상한 東國專門大學 1999 金龜論叢 Vol.7 No.1

최근 버섯은 식용으로서 뿐만 아니라 약용으로서의 기능이 있는 중요한 식품재료로 부각되었다. 우리는 여기에서 최근 식용으로 많이 사용하고 있는 송이, 양송이, 표고, 느타리버섯과, 약용으로서 인식된 아가리쿠스, 상황, 영지, 동충하초에 대하여 이들의 식품으로서의 특수한 기능에 대하여 소개한다. 특히 아직까지 체계적으로 정립되지 않은 이들 약용 버섯 또는 이들 버섯성분의 기능을 보다 더 체계적으로 연구하기 위한 예비단계로서, 여러 가지 항암활성 assay법을 서술하고자 한다. 버섯내의 특이성분에 대한 분자의학적인 mechanism이 밝혀진다면, 암 및 다른 성인병의 치료적인 면에서 버섯류의 역할이 중요하게 인식될 것이다. 미래의 농가소득의 증대와 국민의 보건향상의 측면에서도 이들 버섯산업 및 이와 관련된 의약품개발을 매우 활성화시켜야 하겠다.

천마(天麻, Gastrodia elata Blume) 종균배양의 최적화에 대한 연구

이진만,김성호 東國專門大學 2002 金龜論叢 Vol.9 No.1

천마는 뽕나무버섯과 공생관계로 버섯에 의한 영양공급에 의해서만 생육이 가능한 것으로 알려져 있다. 따라서 천마의 다수확재배를 위한 가장 중요한 인자중의 하나로 우수한 품질의 종균배양은 천마의 재배지의 활착율에 큰 영향을 미치므로 생육 및 수확량, 재배기간 등과 직결된다고 생각된다. 한편 5-6년 전부터 경북 칠곡군 관내에서 대량인공 재배되기 시작하여 그 재배방법에 대한 기초연구가 절실한 실정이다. 따라서 천마재배에 대한 기초연구로서 천마의 종균배양의 최적화를 통해 천마의 다수확재배법의 구축을 위한 기초연구를 수행한 결과, 종균배양에 있어서 액체배양시 물에 용해될 때 친수기 부분이 이온으로 해리되지 않는 계면활성제인 Tween80의 첨가량을 0.0066%, 5mm glass bead ball 30개를 첨가하여 배양시 pulpy growth를 유도할 수 있었으며, 균사체의 질과 양적인 면에서 가장 우수하였다. 따라서 상기의 조건에서 배양한 종균을 참나무 톱밥배지에 5%접종하여 45일간 종균 배양후 25℃, 60%의 습도조건에서 참나무 활착도가 가장 우수하였다.

HP/3000에서 연속적인 값을 갖는 시스템의 모의 실험 방법에 관한 연구

李萬鎬,劉寬鍾,陳成日 충남대학교 자연과학연구소 1981 忠南科學硏究誌 Vol.8 No.2

There are four methods of solving differential equations appearing in engineering problems. First, analytic method. Second, numerical analytic method using digital computer. Third, method using analog computer. Last, method which simulates analog computer by using digital computer processors. In case where me equations are non-linear, the first and the second method are very difficult and the third method is not used well because of the rareness of the analog computer. So it is economical to simulate the analog computer by using digital computer widely used. But, since mere are no such digital-analog simulation processors in HP/3000 computers currently in KOREA, many computer users have difficulties in solving problems. In this paper, we design and implement a simulation processor which enables the fourth method in HP/3000 computers.

씨고자리파리의 에스테라제에 대한 Dichlorvos의 저해효과

이종진,최만영 전북대학교 유전공학연구소 1989 遺傳工學硏究所報 Vol.2 No.1

씨고자리파리의 변태에 따른 단백질 및 에스테라제의 변화, 에스테라제의 활성에 대한 dichlorvos의 영향, 그리고 에스테라제의 α-Naphthylacetate 가수분해 활성에 대한 dichlorvos의 저해효과를 전기영동법과 Gomori의 효소활성 측정법에 의해 조사한 결과 총 28개의 단백질과 14개의 에스테라제 밴드를 분리하였으며, dichlorvos의 중앙치사약량은 암컷성충에서 15.76μg/g, 3령 중기 유충에서 52.17㎍/g이었다. Dichlorvos 처리후 암컷 성충의 에스테라제 밴드의 변화가 관찰되었다. 雌成蟲 homogenate의 전체 esterase 활성중 약 40.9%가 dichlorvos농도 약 6×10^-5M에 의해서 沮害를 받은 것으로 나타났다. Changes in protein and nonspecific soluble esterase patterns, the effect of dichlorvos on esterase activity, and the inhibition of α-Naphthylacetate hydrolyzing activity of esterase by various concentrations of the dichlorvos in the seedcorn maggot, Delia platura(Meigen), were investigated using PAGE and Gomori's colorimetric methods. Through all stages, 28 electrophoretic protein bands and 14 esterase isozyme bands were separated. LD 50 values of the dichlorvos on the larvae and female adults were 52.17μg/g and 15.76μg/g, respectively. Difference of electrophoretic esterase isozyme band patterns between knock-downed, survived and control adults after treatment of dichlorvos were observed. 40.9% of esterase activity to a-Naphthylacetate in homogenate of female adults was inhibitable by 6×10^-5M dichlorvos.