DJ-1/park7 modulates vasorelaxation and blood pressure via epigenetic modification of endothelial nitric oxide synthase

Won, Kyung Jong,Jung, Seung Hyo,Jung, Soo Hyun,Lee, Kang Pa,Lee, Hwan Myung,Lee, Dong-Youb,Park, Eun-Seok,Kim, Junghwan,Kim, Bokyung Oxford University Press 2014 Cardiovascular research Vol.101 No.3

<P><B>Aims</B></P><P>DJ-1/park7, a multifunctional protein, may play essential roles in the vascular system. However, the function of DJ-1/park7 in vascular contractility has remained unclear. The present study was designed to investigate whether the DJ-1/park7 is involved in the regulation of vascular contractility and systolic blood pressure (SBP).</P><P><B>Methods and results</B></P><P>Norepinephrine (NE) elevated contraction in endothelium-intact vessels in a dose-dependent manner, to a greater extent in DJ-1/park7 knockout (DJ-1/park7<SUP>−/−</SUP>) mice than in wild-type (DJ-1/park7<SUP>+/+</SUP>) mice. Acetylcholine inhibited NE-evoked contraction in endothelium-intact vessels, and this was markedly impaired in DJ-1/park7<SUP>−/−</SUP> mice compared with DJ-1/park7<SUP>+/+</SUP>. Nitric oxide (NO) production (82.1 ± 2.8% of control) and endothelial NO synthase (eNOS) expression (61.7 ± 8.9%) were lower, but H<SUB>2</SUB>O<SUB>2</SUB> production (126.4 ± 8.6%) was higher, in endothelial cells from DJ-1/park7<SUP>−/−</SUP> mice than in those from DJ-1/park7<SUP>+/+</SUP> controls; these effects were reversed by DJ-1/park7-overexpressing endothelial cells from DJ-1/park7<SUP>−/−</SUP> mice. Histone deacetylase (HDAC)-1 recruitment and H3 histone acetylation at the eNOS promoter were elevated and diminished, respectively, in DJ-1/park7<SUP>−/−</SUP> mice compared with DJ-1/park7<SUP>+/+</SUP> controls. Moreover, SBP was significantly elevated in DJ-1/park7<SUP>−/−</SUP> mice compared with DJ-1/park7<SUP>+/+</SUP> controls, but this elevation was inhibited in mice treated with valproic acid, an inhibitor of Class I HDACs including HDAC-1.</P><P><B>Conclusion</B></P><P>These results demonstrate that DJ-1/park7 protein may be implicated in the regulation of vascular contractility and blood pressure, probably by the impairment of NO production through H<SUB>2</SUB>O<SUB>2</SUB>-mediated epigenetic inhibition of eNOS expression.</P>

근린공원 환경의 만족도가 신체활동과 건강에 미치는 영향 - 창원시 성산구와 의창구를 대상으로 -

박경훈,이우성,김태환,김은정,Park, Kyung-Hun,Lee, Woo-Sung,Kim, Tae-Hwan,Kim, Eun-Jung 한국조경학회 2014 韓國造景學會誌 Vol.42 No.3

본 연구의 목적은 경상남도 창원시를 대상으로 근린공원 환경에 대한 만족도가 신체활동과 건강증진 목적의 공원이용횟수와 체질량지수(BMI)에 의한 건강수준에 미치는 영향을 분석하는 것이다. 설문조사는 창원시 성산구와 의창구 도심지역에 분포하는 8개 근린공원의 이용자와 그 주변에 거주하는 429명의 주민들을 대상으로 실시하였고, 설문분석은 근린공원 환경에 대한 만족도가 신체활동과 건강에 미치는 영향을 분석하기 위해 일대일대응 선형회귀분석을 활용하였다. 연구결과를 요약하면, 공원을 이용하지 않는 주된 이유는 시간적 여유의 부족, 공원까지의 이동거리와 소요시간, 공원 내 이용시설의 부족 등이고, 공원을 이용하는 주된 이유는 공원까지의 접근성, 걷기 또는 산책, 휴식시설, 수목이나 그늘, 운동공간의 다양성 등으로 나타났다. 공원 이용자의 경우, 공원에서 걷기나 산책을 가장 많이 하고, 주 2회 이상 평균 10분 정도 걸어가서 1시간 정도 운동을 하는 것으로 나타났다. 공원과 그 주변의 물리적 환경 중에서 집에서 공원까지의 거리와 이동하는 가로 환경에 대한 만족도가 높았고, 공원 내 수공간이나 볼거리 등에 대한 만족도가 낮게 나타났다. 다음으로, 운동목적의 공원이용 여부, 공원이용시간, 공원이용의 만족도와 건강증진 효과, 공원까지의 거리, 따른 건강증진 효과만이 유의수준 10% 이내에서 상호관련성이 있는 것으로 나타났다. 향후에도 공원의 신체활동과 건강 증진 효과에 대한 실증적 사례노약자들의 공원이용 편이성 등이 유의수준 5% 이내에서 신체활동과 건강증진 목적의 공원이용횟수에 긍정적인 영향을 보였고, 체질량지수와는 공원이용에 를 지속적으로 축적해 나갈 필요가 있으며, 이를 통해서 각종 개발용도로 전환되거나 그 면적이 축소되고 있는 도시지역의 공원 및 녹지공간을 보전 확대시켜 나가고, 신체활동 및 건강증진 장소로서의 기능을 제고하기 위한 공원계획 설계 요소를 도출하는데 근거 자료를 제공하는 것이 가능할 것으로 판단된다. The purpose of this study is to analyze the effects of satisfaction in neighborhood park environments located in the Changwon-si of Gyeongsangnam-do, South Korea, on physical activity, the number of parks used for health improvement, and health levels based on Body Mass Index(BMI). Accordingly, a survey was conducted of 429 nearby residents and users of eight neighborhood parks located in urban areas of Seongsan-gu and Uichang-gu in the Changwon-si. The correlation between the environmental perception of neighborhood parks and physical activity and health, which was observed in the survey results, was analyzed using one-by-one linear regression analysis. By summarizing the study results, it was found that the primary reasons for avoiding park use were lack of time, time and effort required to reach the park(i.e., distance from the park), and lack of facilities within the park. Conversely, the primary reasons for using the park included accessibility, walking or strolling, leisure facilities, trees or shade, and diversity in exercise areas. In the case of park users, walking or strolling was the most common activity in the park. On average, park users walked to the parks for 10 minutes and exercised for an hour at least twice per week. With respect to the physical environment of the parks and surrounding areas, park users showed a high level of satisfaction with the distance between their houses and parks and the street environment. On the contrary, they exhibited low levels of satisfaction with water spaces and sightseeing within the parks. Subsequently, it was shown that the number of people using the parks for physical activities and health improvement was positively influenced(within a 5% significance level) by the intent to use the park for exercise, time spent in the park, satisfaction with park use and health improvement, distance to the park, and the convenience of using the park for the elderly. However, only the health improvement gained from park use was found to exhibit a correlation with BMI at the 10% significance level. Continuous accumulation of practical case studies on physical activities in parks and their health improvement effects is required. Through this, park spaces that are under the threat of reduction or elimination owing to various development plans can be conserved and expanded. Furthermore, such case studies can be used to provide data as the basis for deriving park plans and designs that improve parks' functions as sites of physical activity and health improvement.

근린공원에 대한 환경지각이 이용자의 건강증진인식에 미치는 영향 - 창원시의 8개 근린공원을 대상으로 -

박영은,이우성,정성관,박경훈,Park, Young-Eun,Lee, Woo-Sung,Jung, Sung-Gwan,Park, Kyung-Hun 한국조경학회 2015 韓國造景學會誌 Vol.43 No.1

본 연구는 근린공원에 대한 이용자의 공원지각환경이 건강증진인식에 미치는 영향을 분석하기 위해 경상남도 창원시의 8개 근린공원을 대상으로 공원별 현장조사 및 설문조사를 실시하였다. 연구결과에 따르면, 먼저 공원별 공원지각환경에서는 대부분의 공원이 집에서 공원까지의 거리와 집에서 공원까지의 길 환경이 높게 평가된 반면, 수공간과 공원 내 다양한 볼거리는 충분하지 못한 것으로 분석되었다. 다음으로 23개의 지각환경 변수들을 요인분석한 결과, 경관성, 안락성, 접근성, 활동성, 편의성, 쾌적성의 6개 요인으로 분류되었다. 이를 토대로 건강증진인식과 지각환경요인과의 관련성을 분석한 결과, 8개 공원 중 4개 공원에서 지각환경요인이 신체적 건강증진인식에 유의한 영향을 주는 것으로 나타났다. 특히, 접근성과 활동성이 3개의 공원에서 영향력이 있는 변수로 분석되었다. 또한, 심리적 건강증진인식의 경우, 8개 공원 중 5개 공원에서 유의한 영향을 주는 것으로 나타났으며, 접근성이 4개의 공원에서 영향력이 있는 변수로 평가되었다. 이상과 같은 결과들은 기존의 공원 환경을 개선하고, 새로운 공원을 조성하고자 할 때 효율적인 기초자료로 활용될 수 있을 것으로 판단된다. The purpose of this study was to analyze the effect of environmental perception in neighborhood parks on user's recognition of health improvement. This study used objective field survey data and subjective user survey data at 8 neighborhood parks in Changwon City. According to the results, the perceptions of distance and street environment from home to park were evaluated highly but the perception of water spaces and various attractions within the parks were rated lowly in most of parks. As a result of factor analysis, 23 environmental perception variables were classified into 6 factors such as scenery, comfort, accessibility, activity, convenience and amenity. For the result of regression analysis between environmental perceptions and user's recognition of health improvement based on 6 factors, the recognition of physical health improvement was significantly influenced from environmental perception factors in 4 parks among 8 parks. 'Accessibility' and 'activity' were analyzed as the meaningful factors in 3 parks. Also, recognition of mental health improvement was significantly influenced from environmental perception factors in 5 parks among 8 parks and especially 'accessibility' was showed as the significant factor in 4 parks. The findings from this study can contribute to improve the physical environments of the present parks and can be a basic data for new park development.

박춘무의 패션 디자인에 나타난 미적 특성 - 2010 FW ~ 2017 SS 컬렉션을 중심으로 -

박은경 ( Eun Kyung Park ) 한국디자인트렌드학회 2018 한국디자인포럼 Vol.23 No.4

연구배경 최근 세계적으로 한국 패션에 대한 관심이 높아지면서 한국 패션의 역사 기록과 특성에 대한 연구가 중요해지고 있다. 본 연구는 국제와 국내 무대에서 활발히 활동하고 있는 한국 중견 패션 디자이너 박춘무의 작품을 분석하고 디자인의 미적 특성을 밝혀 한국 현대 패션 역사의 일부를 기록하고 한국 패션의 정체성을 논의할 수 있는 자료를 제공하고자 하였다. 연구방법 본 연구는 인터넷 검색 사이트를 이용하여 박춘무 디자이너와 그의 컬렉션, 디자인에 대한 기사와 리뷰를 수집하였고, 그가 뉴욕패션위크에 참가한 2010 FW~ 2017 SS까지의 컬렉션 사진자료를 수집하여 디자인 특성을 실증적으로 분석하였다. 사진 자료는 여러 패션전문 매체 인터넷 사이트를 이용하여 수집하였고 컬렉션 주제와 영감, 조형 특성, 이미지를 분석하였다. 그리고 이를 기반으로 디자이너 작품세계의 미적 특성을 총체적으로 논의하였다. 연구결과 박춘무 관련 기사와 컬렉션 작품을 분석한 결과 그의 디자인에서 반복적으로 나타나는 대표 디자인 특성은 무채색과 모노톤, 구조적 라인과 비정형적 라인의 조화, 소재 대비와 혼합에 의한 조형 표현, 미니멀리즘, 모호한 성의 표현, 스포티즘, 그리고 한국적인 미학의 이미지 표현으로 나타났다. 결론 30년의 역사를 지닌 브랜드 디자이너 박춘무는 비교적 일찍부터 해외 진출을 시도하여 성과를 거두었고, 상업성과 디자이너 감성의 조율을 통해 성장하였다. 그는 아방가르드와 모던한 디자인 감성을 기본으로, 최근에는 한국적 감성의 도입을 시도하고 있다. 본 연구결과 박춘무의 컬렉션에 나타난 디자인의 미적특성은 ‘컨템포러리 아방가르드’,‘한국적 미학의 아방가르드’로 결론내릴 수 있었다. Background Recently, the record and research on the characteristics of Korean fashion is becoming important. The purpose of this study is to analyze the works of Korean fashion designer Park Choonmoo, who is actively engaged in international and domestic stage, to reveal her design aesthetic features. Methods This study collected articles and reviews on Park Choonmoo and her fashion collection, using an internet search site. And study analyzed the design of collection from 2010 FW to 2017 SS in New York Fashion Week. Collection photographs were collected from various fashion media web sites and brand facebook. The collection theme, inspiration, formative characteristics, and images were analyzed. Result The characteristics of Park’s design appeared as achromatic and monotone, harmony of structural line and irregular line, formative expression by mixture of material and material. Also images of minimalism, ambiguous sex expression, sportism, and expression of Korean aesthetics was appeared. Conclusion Park Choonmoo has been based on avant-garde and modern design sensibility, and recently she is trying to introduce Korean sensibility. As a result of this study, the aesthetic characteristics of the design shown in Park's collection appeared as ‘contemporary avant-garde' and ‘Korean aesthetic avant-garde’.

RAW264.7 세포에서 interferon-r 및 LPS에 의해 유도되는 NO생성에 미치는 TALT-35의 영향

박종일,박경석,김종석,박지훈,윤은진,송경섭,서강식,김훈,윤완희,박승길,임규,황병두 충남대학교 생물공학연구소 2006 생물공학연구지 Vol.12 No.-

TALP-35 purified from human term placenta is known to increase microtubule polymerization and stabilize the polymerized microtubule. To examine the effect of TALP-35 on immune system this study was performed. MTT assay was performed to investigate the effect of TALP-35 on the proliferation of RAW264.7 cells. TALP-35 dose dependently suppress the proliferation of RAW264.7 cells at high concentration (above 1 μM) in unstimulated cells, in case of 10 μM TALP-35 treated cells the suppression was 25% but in stimulated cells it was only 15%. Cosedimentation assay was carried out to investigate whether TALP-35 can bind to tubulin of RAW264.7, monocyte/macrophage lineage of mouse, and polymerize it. TALP-35 polymerize the tubulin of RAW264.7 cells and sedimented in dose-dependent manner. To investigate the effect of TALP-35 on the expression of iNOS protein western blotting was performed. The expression level of iNOS was decreased dose dependently in high concentration of TALP-35 treatment. To examine the activity of iNOS, secreted NO was determined by method based on Griess reaction. Interferon-γ and LPS-stimulated production of NO from RAW264.7 cells was decreased dose dependently above 0.1 μM concentration of TALP-35 and 50% is decreased at 10μM of it. This study shows TALP-35 can control cytokine induced-iNOS expression therefore it might control inflammatory diseases.

DJ-1 deficiency impairs synaptic vesicle endocytosis and reavailability at nerve terminals

Kyung, Jae Won,Kim, Jin-Mo,Lee, Wongyoung,Ha, Tae-Young,Cha, Seon-Heui,Chung, Kyung-Hwun,Choi, Dong-Joo,Jou, Ilo,Song, Woo Keun,Joe, Eun-Hye,Kim, Sung Hyun,Park, Sang Myun National Academy of Sciences 2018 Proceedings of the National Academy of Sciences Vol.115 No.7

<P>Mutations in DJ-1 (PARK7) are a known cause of early-onset autosomal recessive Parkinson's disease (PD). Accumulating evidence indicates that abnormalities of synaptic vesicle trafficking underlie the pathophysiological mechanism of PD. In the present study, we explored whether DJ-1 is involved in CNS synaptic function. DJ-1 deficiency impaired synaptic vesicle endocytosis and reavailability without inducing structural alterations in synapses. Familial mutants of DJ-1 (M26I, E64D, and L166P) were unable to rescue defective endocytosis of synaptic vesicles, whereas WT DJ-1 expression completely restored endocytic function in DJ-1 KO neurons. The defective synaptic endocytosis shown in DJ-1 KO neurons may be attributable to alterations in membrane cholesterol level. Thus, DJ-1 appears essential for synaptic vesicle endocytosis and reavailability, and impairment of this function by familial mutants of DJ-1 may be related to the pathogenesis of PD.</P>

저체중 또는 과체중 청소년에서 캠프 프로그램을 이용한 영양 교육효과에 관한 연구

성미경,승정자,류화춘,박재년,박동연,최미경,조경옥,최선혜,이윤신,김유경,이은주 대한지역사회영양학회 2003 대한지역사회영양학회지 Vol.8 No.4

This study was performed to evaluate the effectiveness of a nutrition education program developed for nutritionally imbalanced adolescents. A summer nutrition camp was held for 23 overweight and 16 underweight subjects. Its effectiveness with regard to was evaluated at the end of the camp and 6 months later. Nutrition knowledge, nutritional attitude, food behavior, nutrient intake, exercise habit and ideal body figures desired by the subjects. The results showed significantly higher nutrition knowledge scores at the end of the camp as compared to those obtained prior to the camp program, and these scores were maintained for at least six months. Nutrition attitude scores also improved after the education program, and these improved scores also lasted for 6 months. However, the food behavior scores measured 6 months after the education program were not significantly different from those obtained prior to the camp. Also, the exercise habit, the ideal body figures and the body figures desired by the subjects remained unchanged. When nutrient intakes of subjects were assessed before the program and 6 months later, the mean daily vitamin C intake was significantly increased after the education program. Also, the intake of iron from plant food sources increased in the overweight subjects, while less iron from animal source were consumed by the underweight subjects. Both groups tended to consume more vegetables and fruits 6 months after the education program which may have contribute to the higher vitamin C and plant-based iron intakes. These results indicate that a 4-day nutrition education camp program sustained changes in nutrition knowledge and nutrition attitude for 6 months. The increased intake of vegetables and fruits was also achieved through this education program. However, changes in dietary behavior in adolescents may require repeated education.

심근 허혈-재관류 백서 모델에서 mitogen-activated protein kinase 활성화에 대한 α-lipoic acid의 효과

김연경,박은미,최준호,윤경호,유남진,이은미,김남호,정진원,김민선,박병림,오석규 圓光大學校 醫科學硏究所 2006 圓光醫科學 Vol.21 No.1

제초제 MCPP에 의한 화학적 화상

김은경 ( Eun Kyung Kim ),김혜성 ( Hei Sung Kim ),박영민 ( Young Min Park ),김형옥 ( Hyung Ok Kim ),이준영 ( Jun Young Lee ) 대한피부과학회 2011 대한피부과학회지 Vol.49 No.10

MCPP (2-(4-chloro-2-methyl phenoxy) propionic acid) is a phenoxy herbicide found in many household weed killers and lawn fertilizers. Some of the phenoxy acids, salts, and esters are moderately irritating to skin, eyes, and respiratory and gastrointestinal linings. In a few individuals, local depigmentation has apparently resulted from protracted dermal contact with phenoxy compounds. Although acute irritant contact dermatitis induced by herbicides is common, chemical burns induced by herbicides have been reported infrequently in the literature. Here we describe a chemical burn in a 25 year old female after sitting on a lawn that had been sprayed with MCPP 3 days earlier. This case illustrates potential hazards of the herbicide on the lawn of a public park. (Korean J Dermatol 2011;49(10):936∼939)

Repellent efficacy of essential oils and plant extracts against Tribolium castaneum and Plodia interpunctella

Lee Hwa‐Eun,Hong Su Jung,Hasan Najmul,Baek Eun Joo,Kim Jun Tae,Kim Young‐Duk,Park Mi‐Kyung 한국곤충학회 2020 Entomological Research Vol.50 No.9

This study was conducted to investigate the repellent efficacy of essential oils (Origanum vulgare, Pimpinella anisum, and Tanacetum cinerariifolium) and four plant extracts (Agastache rugosa, Capsicum annuum, Citrus reticulata, and Ginkgo biloba) against Tribolium castaneum (adults and larvae) and Plodia interpunctella (larvae). Gas chromatography/mass spectrometry analysis revealed the presence of carvacrol, anethole, and jasmolin I as the predominant constituent in O. vulgare, P. anisum, and T. cinerariifolium, respectively. Furthermore, ethyl hexopyranoside, 9,12‐octadecadienoic acid, cyclopentanol, and 2‐cresol were identified in A. rugosa, C. annuum, C. reticulata, and G. biloba, respectively. The repellent efficacy of each essential oil, plant extract, and the combination of oils was evaluated using a specially designed cylinder trap for 120 h. Among the three oils, O. vulgare and T. cinerariifolium had greatest repellent efficacy against P. interpunctella larvae. T. cinerariifolium exhibited effective repellence against the adults and larvae of T. castaneum. Therefore, O. vulgare (O) and T. cinerariifolium (T) were selected for further investigation of combined effects. Two essential oils were mixed in three different ratios of OT1 (1:3), OT2 (1:1), and OT3 (3:1). The repellent efficacies of OT1 and OT2 against the adults of T. castaneum were significantly greater than that of OT3. OT1 was effective against the larvae of T. castaneum, whereas OT2 was effective against the larvae of P. interpunctella. OT1 enhanced the repellent efficacy by approximately five times against larvae of T. castaneum, compared with that of T. cinerariifolium. Overall, OT1 was selected as the best repellent substance against all the tested insects.