비평형제2상의 용체화거동에 미치는 응고조직의 영향

윤의박,안갑우,서창제,허태수 대한금속재료학회(대한금속학회) 1980 대한금속·재료학회지 Vol.18 No.5

偏析型 2元系合金의 溶體化末期에 있어서 非平衡第2相의 固溶擧動에 대해서는 아직까지 理論的으로 정립되어 있지 않았다. 本 論文에서는 主로 dendrite element의 境界에 晶出된 非平衡第2相의 유효擴散距離로써 1次arm 間隔을 考慮한 擴散 model인 尹의 近似解에 의해 이들 擧動을 調査하였다. 이를 위해 天然가스등의 輸送材料로 囑望되는 高濃度 Al-8%Mg合金을 사용하였다. 實驗結果, 理論的인 尹의 近似解의 實驗値는 近似的으로 一致되었고, 특히 溶體化末期에서는 完全히 一致됨을 알 수 있다. 結局 從來의 Flemings의 解析値보다 더욱 實驗値에 近似하였다. The solution kinetics of nonequilibrium second phase of a segregated binary alloy in the later stages of solution treatment have not yet been made theoretically clear. This paper attempted mainly to investigate this behaviour by Yoon's approximate analysis given by a simple model based upon primary arm spacing as an effective diffusion distance of the second phase which has crystallized at the boundary of dendrite element. For this purpose, we used the high magnesium Al-8% Mg alloy which is expected to be used for transport of liguified natural gas. The theoretically calculated results are in the approximate agreement with that of experiments for complete dissolution time and solution kinetics (expecially at the later stages) of β-phase. The Yoon's approximate analysis was in better agreement with the present experimental results than the Flemings conventional analysis

Micropropagation of Plants and Mass Production of Adventitious Roots from Culture of Seedling Explants of Polygonatum odoratum

Yoon,Eui-Soo 한국자원식물학회 1998 한국자원식물학회지 Vol.11 No.4

When the leaves, roots and stem segments of seedling of Polygonatum odoratum were cultured on Murashige and Skoog medium with 2.0mg/l BAP, stem segments were the most efficient explants for adventitious shoot inductino. To observe the efficient combination of growth regulators on the adventitious shoot formation , stem segments were cultured on MS medium with various kinds of cytokinins (BAP, kinetin, zeatin). From this experiment, cytokinin treatement was prerequisite for theadventitious shoot formatino,especially BAP was the most effective. Auxin (NAA or IBA) in combination with cyotokinin highly enhanced the adventitious shoot formation. Twenty five percents of explants produced the adventitious shoots on medium with 2.0mg/l BAP solely, while 83% of explants produced the adventitious shoots on medium with 2.0mg/l BAP and 0.1mg/l IBA. Root formationform adventitious shoot was promoted after transfer to 1/2 MS medium supplemented with 0.1mg/l IBA and 0.5mg/l zeatin, thereafter the plantlets with shoots and roots were cultured on 1/2MS medium lacking growth regulators. When the stem segments were cultured to MS medium with 1.0mg/l 2,4 NAA and IBA , yellow and nodulous cali were formed from the stem segments which were developed into adventitious roots. These roots were actively grew after transferred to MS liquid medium lacking growth regulators.

Fordyce Granules

박의수,송준영,최윤애 啓明大學校 醫科大學 1993 계명의대학술지 Vol.12 No.3

Fordyce granules are small yellow spots that are multifocal and slightly elevated. They may occur in clusters or coalesce to from yellow plaques. They are bilaterally symmetrical and are located most frequently on the buccal mucosa of the cheeks, the inner surface of the lips, and on the mucosa distal to the mandibular molars. Fordyce spots are extremely common: probably 80% of the population have them. They are often not noticeable in children until after puberty, and they seem to be more obvious in males, in patients with greasy skin and in the elderly. The granules are totally benign, though the occasional patient or physician becomes concerned about them or misdiagnoses them as thrush, lichen planus or other diseases. We present two cases of Fordyce granules a 64 and a 50 year-old male who have cancer phobia, showing multiple involvement of minute yellowish white colored spots on the buccal mucosa and inner surface of the lip. Histopathologic finding shows a group of small but mature sebaceous lobules situated around a small sebaceous duct leading to the surface epithelium. We did not give any specific treatment but gave reassurance, presently under observation.

유도 '되치기 본'의 개선 방안에 관한 연구

김의환,김도준,김규수,김관현,김종달,최종삼,조용철,박순진,윤익선,안병근,정 훈,김미정,한성철 龍仁大學校 武道硏究所 1999 武道硏究所誌 Vol.10 No.1

The purpose of this study was to reform practically a Korean Judo's Doechigi-Bon(Forms of Counterattack, judo's Gaeshi no Kata, Judo's Uradori no Kata, Judo's Gonosen no Kata) that was established in 1955 Korea, according to changing of techniques by Judo's modernization, in order to have Judo's carefulness and systematic diffusion. Reform procedure of Judo's Doechigi-Bon was 1st stage, Questionnaire survey 303 judokas, 2nd stage, Technical seminar by judo experts(12 judo professor) 4 times, 3rd stage, wording report for reform, 4rd stage, Discussion and judgement of Teaching and Judgement commission of Korean judo Association(KJA), 5th stage, Public hearing for reform in KJA, 6th stage, Report and decision of board of directors in KJA, 7th stage, public publication of Judo News(No.53) in KJA. Basic principle of reform of Judo's Doechigi-Bon were as table 1. Table 1. Basic principle of reform of Judo's Doechigi-Bon ------------------------------------------------------------------------------- Items Reformed Key Points of Judo's Doechigi-Bon ------------------------------------------------------------------------------- 1. Structure 1) Grand classification : Classified by 3 parts(1,2,3Gyo) 2)Medium classification : Te waza, Goshi waza, Ashi waza devided per each part(Gyo) 3)Sub-classification : Classified five techniques per each part(Gyo) 2. Contents 1) Selected established techniques as possible 2) Considered rationalty and overlapping of counterattack techniques 3. Decision of Conterattack techniques 1) Refered to results of Basic Questionnaire survey 2) Priority to decisions of Judo expert technical seminar -------------------------------------------------------------------------------- Reformed Korean Judo's Doechigi-Bon(Forms of Counterattack techniques-KJA, 1999) are as follows: 1. Gyo; ① Uki otoshi -> Uchi mata ② Harai goshi -> Harai goshi gaeshi ③ O soto gari -> O soto gake ④ Ko uchi gari -> Sasae tsurikomi ashi ⑤ O uchi gari -> Ko soto gari 2. Gyo; ① Ippon seoi nage -> Okuri eri jime ② Tsuri domi goshi -> Uki waza ③ Okuri ashi harai -> Okuri ashi harai ④ Ko soto gari -> Tai otoshi ⑤ Hiza guruma -> Hiza guruma 3. Gyo; ① Kata guruma -> Sumi gaeshi ② Tai otoshi -> Ko soto gari ③ Hane goshi -> Harai tsurikomi ashi ④ Uchi mata -> Tai otoshi ⑤ Tomoe nage -> O uchi gari

두부회전에 따른 측모두부방사선 계측치의 변화

김광수,황미선,최의환,김광원,윤영주 대한치과교정학회 2000 대한치과교정학회지 Vol.30 No.1

본 연구는 측모두부방사선사진 촬영시 발생될 수 있는 두부회전이 측정된 선, 각계측치들에 어느 정도의 투사오차를 야기시키는지 알아보기 위해 조선대학교 의과대학 해부학교실에 소장중인 건조두개골 중 비교적 상태가 양호하고 특별한 비대칭이 없는 영구치열기의 건조두개골 17개를 표본으로 선택하여 시행하였다. 각각의 건조두개골을 수직축(Z축)을 중심으로 기준위치(0˚)에 대해 1˚ 간격으로 ±15˚ 까지 실험적으로 회전시켜 총 527장의 측모두부방사선사진을 촬영하였다. 이를 근거로 기준위치(0˚)에서의 계측치와 각 회전각에서의 계측치들 사이에 paired t-test를 시행하여 측모두부방사선사진 계측치 간의 차이를 규명하였으며, 이를 통해 투사오차의 관점에서 교정학적으로 유용한 측모두부방사선 계측항목을 구한 결과 다음과 같은 결론을 얻었다. 1.각계측항목이 선계측항목에 비해 투사오차가 작았다. 2.각계측항목은 정중시상면에 위치한 기준점들을 많이 포함할수록 투사오차가 작았다. 3.수평선계측항목의 길이는 필름방향으로 회전됨에 따라 점진적으로 감소되었으나, 초점 방향으로 회전됨에 따라서는 증가되다가 감소되었으며 상대적으로 그 변화양이 작았다. 4.두부회전에 따른 투사오차는 수직선계측항목에 비해 수평선계측항목에서 컸다. 5.수직선계측항목은 회전축으로부터 거리가 증가함에 따라 투사오차가 증가하였다. 이상을 종합해 볼 때 두부회전에 따른 측모두부방사선사진 계측치의 투사오차를 최소로 하기 위해서는 선계측항목보다는 각계측항목을 사용하는 것이 유용할 것으로 사료된다. This study was performed to find out the effect of projection errors on cephalometric linear and angular measurements according to head rotation during taking lateral cephalometric radiographs. Seventeen skulls with permanent dentition and no gross asymmetry were obtained from the Department of Anatomy, Medical School, Chosun University. Total 527 x-ray films were taken with 1˚ interval from the reference position(0˚) to ±15˚ around the vertical axis (Z axis) which is perpendicular to the midpoint of the line connecting the center of two ear rods in submento-vertex direction. Statistical analysis was performed by faired t-test if there were statistically significant differences between the mean of the reference position(0") and that of each rotation angle. The following results were obtained. 1.The projection errors of angular measurements were smaller than those of linear measurements. 2.The projection errors of angular measurements including midline landmarks were smaller than those including bilateral landmarks. 3.The horizontal linear measurements were gradually decreased when the skull was rotated toward the film, but slightly increased and then decreased when the skull was rotated toward the focal spot. However, the changes were smaller in focal direction. 4.The projection errors of horizontal linear measurements were larger than those of vertical linear measurements. 5.The projection errors of vertical linear measurements were increased with increased distance from the rotation axis to vertical measurements. It is concluded that the use of angular measeurements rather than linear measurements is recommended to minimize the projection errors.

터널 및 地下採掘 現場에서의 V-CUT심빼기를 應用한 長孔發破에 對한 硏究

金益壽,鄭義鳳,金琮鈗,金鎭錫 三陟大學校 産業科學技術硏究所 2002 産業科學技術硏究論文集 Vol.7 No.1

This research is on developing a new blasting technique which will complement the problems of the ordinary burn-cut blasting technique. This new technique employ a v-cut long hole blasting technique. the on-site testing of this v-cut technique has shown a number of advantages to the ordinary burn-cut blasting technique. When tested on the whole-section digging on the large section of a standard mining hole. the v-cut technique has shown the equivalent result to the ordinary burn-cut technique. However, the v-cut technique is more effective than the burn-cut technique, since it makes drilling simpler, and reduces both cost and amount of construction time.

Effect of Ionizing Radiation on Rat Tissue: Proteomic and Biochemical Analysis

Park*, Eui‐,Chul,Yoon, Jong‐,Bok,Seong*, Jin‐,Sil,Choi, Kyoung‐,Soo,Kong, Eung‐,Sik,Kim, Yun‐,Jeong,Park, Young‐,Mee,Park, Eun‐,Mi Taylor Francis 2006 Preparative biochemistry & biotechnology Vol.36 No.1

<P>Reactive oxygen species (ROS), generated by ionizing radiation, has been implicated in its effect on living tissues. We confirmed the changes in the oxidative stress markers upon irradiation. We characterized the changes in the proteome profile in rat liver after administering irradiation, and the affected proteins were identified by MALDI-TOF-MS and ESI-MS/MS. The identified proteins represent diverse sets of proteins participating in the cellular metabolism. Our results demonstrated that proteomics analysis is a useful method for characterization of a global proteome change caused by ionizing radiation to unravel the molecular mechanisms involved in the cellular responses to ionizing radiation.</P>

高等學校 生物의 植物組織培養 實驗 Model

兪哲濬,尹義洙,金鍾均 공주대학교 사범대학 과학교육연구소 1992 과학교육연구 Vol.23 No.1

본 연구는 제5차 교육과정에 따른 인문계 고등학교 교과서에 소개된 조직 배양의 내용을 비교 분석한 결과 및 기초 실험의 결과를 토대로 다음과 같은 결론을 내리고 생물 교육 개선을 위한 자료로 삼고자 한다. 제3차 교육과정 이후 제4차 교육과정으로 개편하면서 조직배양에 대한 내용이 더욱 강화되었으며 그의 이용부분까지도 설명하고 있다. 또한 더욱 발전될 것을 제시하여 학생들의 탐구력 신장 및 흥미를 갖게 하였다. 인문계 고교 과학Ⅰ(상)에서 조직 배양에 관한 사항을 이용하는 단원은 5개 단원 모두에 해당되고 있다. 또한 생물 교과서에서도 5개 단원 중 3개 단원에 조직 배양에 관한 사항이 이용되고 있다. 실물 조직 배양의 교재적 의의는 매우 크다. 더우기 발전된 실험을 행할 경우 Biotechnology로써 유전자 교환, 세포융합, 형질 전환 등의 실험은 많은 학생에게 흥미와 관심을 갖게 할 것이다. 조직 배양에 사용되는 기구와 기재는 현재 학교에서 사용하는 실험 기구와 기재를 이용할 수 있으나 몇 몇 기재는 간이 기구 및 다른 방법으로 이용할 수 있으므로 별도의 구입은 필요로 하지 않는다. 조직 배양시 필요로하는 화합물 약 30여종으로 되어 있으나 거의 대부분 학교에 비치되어 있고 비타민류, 아미노산류, 식물호르몬류는 별도로 구입을 하여야 하나 아주 미량으로 사용되므로 많은 비용이 요구되지 않는다. Hyponex배지를 사용할 경우에는 화합물 준비는 더욱 용이하다. 조직 배지를 상품화한 것을 이용하여 실험할 수 있게 하는 것이 필요하다. Model화된 식물 조직 배양의 실험 방법은 현재 고등학교에 있는 기구를 이용하면 가능하다. 다소 어려움이 있더라도 학생에게 조금이라도 실제로 행하도록 하거나 관찰시키는 기회를 많이 갖게 하는 것이 바람직하다. 이렇게 하여 자연과학에 대한 흥미와 관심을 높여가는 것이 초 ·중 ·고를 통하여 증시되고 있는 탐구과정에도 상통하는 것이다. Model화를 위한 기초 실험에서 나타난 문제점을 간이 방법 및 간이 기구로 해결하여 실험을 하였으나, 좋은 재료와 기구 및 방법으로 실시하도록 많은 연구가 필요할 것으로 생각된다. This study is meant as a teaching aid for the improvement of biology as follows on the basis of the compared and analyzed result of the content on the tissue sulture introduced in cultural high school texts by the fifth curriculum. The third curriculum has been reorganized into the fourth and fifth, during which the presenting the further development students are enabled to have the expansion of research and interest. The items concerning tissue culture are contained in the five units of cultural high school science I, and in the three among five units of biology text, and tissue culture as a text counts for much and in case of developed experiment the experiment on the exchange of gene as a biotechnolgy, cell fusion and transformation are expected to interest many students. The instruments and material used for tissue culture can be replaced with the preequipped ones in school laboratory and some tools need not purchasing as some temporary tools can be substituted for them. The complex needed in tissue cult-ure consists of about thirty types, most of which re equipped in almost all schools and vitamins, amino acids and plant hormones which need seperate purchase are used in quite a fraction and so don`t require much expense. The use of hyponex medium makes it easier to prepare complex. It is necessary that tissue culture medium produced on a commercial sale should be used in experiment. The model of experiment in plant tissue culture can be conducted using the present tools and instrument and it is desirable that students perform themselves and observe. By doing so, the promotion of the interest in natural science is consistent and universal in the course of research valued in the primary and secondary school curriculum. The issues presented in the basic experiment for modelling are experimented by using some temporary methods and instruments, many studies are thought to be done using more favorable material and instrument.

無酸素性 運動能力과 生理學的 變因의 關聯性

김기진,윤성원,김학열,정정진,우도영,노성규,손태열,안의수 江原大學校附設 體育科學硏究所 1991 江原大學校附設體育科學硏究所論文集 Vol.- No.16

Wingate test 및 Supramaximal exercise를 이용한 무산소성 운동능력의 측정시 나타난 변인의 관련성을 살펴본 본 연구를 요약하면 다음과 같다. Wingate test시 혈중젖산농도 최고치는 9.04±0.62mM로서 Supramaximal exercise시 보다 유의하게 높았으며, 혈장 LDH 및 CPK활성도는 두가지 운동부하후 모두 유의한 변화는 나타나지 않았다. 근전도상의 적분치, 중간파형빈도 및 평균파형빈도도 유의한 변화는 없었다. 등속성 수축시의 최대토오크는 양쪽 다리 모두에서 초당 60도의 각속도에서가장 높았다. Wingate test시의 최대파워는 등속성 수축시 최대토오크 및 Wingate test시 중간파형빈도의 직접적인 영향을 받는 것으로 나타났으며, 평균파워는 Wingate test시의 적분치 변화, 듣는 쪽 슬관절의 등속성 수축시최대토오크 및 Supramaximal exercise시 혈장 LDH 활성도의 직접적인 영향을 받는 것으로 나타났다. 이상의 결과에서 무산소성 운동능력과 관련지어 그 기전을 살피고자 할 때 체내 화학적 변화, 전기생리적 변화 및 발현능력의 복합성을 고려해야 하며 최대누적 산소결핍량의 활용가능성은 측정강도 결정방법을 중심으로한 계속적인 연구가 요구된다. The present study examined the relationship of variables related to anaerobic capacity with Wingate test and supramaximal exercise. Peak value of blood lactate concentration with Wingate test showeda significantly higher than suprammaximal exercise, and plasma LDH & median power frequency and mean power frequency were not any difference between two tests. Peak torque of isokinetic contractionh on knee joint showed a highest value in 60 degrees of angular velocity among a various angular velocity. Peak power of Wingate test was related to peak torque of isokinetic contraction and median power frequency of EMG with Wingate test, and mean power of Wingate test was related to integrated EMG with Wingate test, peak torque with isokinetic contraction of dominant knee joint and plasma LDH activity with supramaximal exercise. In this result, when it analyzed the mechanism of anaerobic capacity, ti was required the consideration of complexity according to biochemical alteration, electrophysiological alteration and revelation of performance, Appicapability of maximal accumulated O₂deficit was demaned a continuous study with the focus of exercise intensity.

高等學校 生物의 遺傳工學 槪念 導入을 위한 實驗 Model

박상익,윤의수,김종균 공주대학교 사범대학 과학교육연구소 1995 과학교육연구 Vol.26 No.1

제 4차 교육과정 이후 제 5차 교육과정으로 개편되면서 생물 교과서에 유전 공학 개념 및 응용면이 더욱 강조 되었고, 제 6차 교육과정에서는 더욱 심화된 내용이 소개될 것으로 본다. 특히 일본의 고교 생물에서는 유저너 공학에 관한 내용을 학생들이 실험 할 수 있게 수록되어 있다. 그리고 앞으로의 자연과학은 유전공학 분야에서도 눈부신 발전을 가져올 것으로 기대되면 이에 맞추어 고등학교에서 유전공학 개념의 이해를 위한 유전자의 삽입, 형질 전환, 조직 배양 등의 실험을 실시하는 것은 학생들에게 많은 흥미와 관심을 갖게 하며 탐구력 신장에도 도움을 줄 것으로 생각한다. 본 연구는 제 5차 교육과정에 따른 고등학교 생물 교과서에 소개된 유전공학 내용을 분석한 결과 및 잎 절편체가 모상근으로 형질전환되는 기초 실험의 결과를 토대로 다음과 같은 결론을 내리고 탐구적 생물교육 자료로 삼고자 한다. 1.Agrobacterium tumefacieus, Agrobacterium rhizogenes을 이용한 모상근 발생에 관한 기초 실험의 특징은 형질전환체를 가시적인 뿌리로 얻을 수 있어 관찰이 용이하다. 2.형질 전환이 되었음을 GUS 활성 검사에 의해 쉽게 증명할 수 있어 유전공학 개념 도입을 위한 실험으로 고등학교에 도입하는 데 어려움이 없을 것으로 본다. 3.실험에 이용한 균은 감염 부위에 모상근을 형성하는 이외는 다른 병증을 나타내지 않기 때문에 대외적인 문제 없이 학교의 실험실에서도 취급할 수 있다. 4.유전 공학 실험에 필요로하는 화합물은 일선 학교에 대부분이 준비되어 있으며 kanamycin, Claforan은 미량 필요한데 쉽게 적은 비용으로 구입할 수 있다. 5.실험 기구는 간이 배양실만 고안 사용하면, 고등학교에 준비되어 있는 기구를 이용하여 해결이 가능하다. 유전 공학에 관한 실험을 하는데 실험실의 환경 조건이나 다인수 학급에서 어려움이 있더라도, 학생들에게 실험을 통해 유전 공학 개념을 형성시키는 기회를 갖게하는 것은 매우 중요하다. 이렇게 하여 자연과학에 대한 흥미와 관심을 높여가는 것은 우리나라의 기초과학 발전에 도움이 될 것으로 본다. The concept of biotechnology and its practical application is much more emphasized in the fifth curriculum than in the fourths and further intensified contents are supposed to be introduced in the sixth. Especially highschool biology in Japan, the contents on the biotechnology are recorded for the students to experiment and the future natural science is expected to develop brilliantly in a field of biotechnology. Therefore, students' experiments on gene insertion, transformation, tissue culture and so on, for their understanding of biotechnology concepts, are thought to stimulate their curiosity and extend their spirit of inquiry. This study is meant as an teaching aid, and reaches the following conclusions on the basis of the results of the biotechnology contents introduced in the fifth highschool biology texts and those of basic experiments on the transformation from explant to hairy root. The characteristic of basic experiments on hairy root growth used by Agrobacteriurm tumefacieus, Agrobacterium rhizogenes is that the observation is easy, since the transformational object is a visible root. The experiments are likely to be introduced without difficulty for highschool biotechnology concepts, since the transformation is easily proved by the GUS activation test. The germs used in experiments don't cause other diseases except forming hairy roots in infected parts, so they can be treated in school laboratories without outside interferance. Almost all schools are equipped with the complex materials needed for biotechnology experiments and kanamycin and claforan, just a little quantity of which is needed, can be purchased at a low price. The instruments can be replaced with the preequipped ones in school, only the temporary growthchamber needs to be devised. Though there are difficulties in biotechnology experiments because of unsufficient condition in laboratories or classes made up of a large number of students, it is very important for the students to have opportunities to form biotechnology concepts through experiments. To promote curiosity and interest in natural science in this way can be helpful in national basic science development.