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- 저자 : ( Zhi-qiang Song ) (검색결과 22 건)
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Song, Zhi-Qiang,Cheng, Ju-E,Cheng, Fei-Xue,Zhang, De-Yong,Liu, Yong The Korean Society of Plant Pathology 2017 Plant Pathology Journal Vol.33 No.2
Tylenchulus semipenetrans is an important and widespread plant-parasitic nematode of citrus worldwide and can cause citrus slow decline disease leading to significant reduction in tree growth and yield. Rapid and accurate detection of T. semipenetrans in soil is important for the disease forecasting and management. In this study, a loop-mediated isothermal amplification (LAMP) assay was developed to detect T. semipenetrans using DNA extracted from soil. A set of five primers was designed from the internal transcribed spacer region (ITS1) of rDNA, and was highly specific to T. semipenetrans. The LAMP reaction was performed at $63^{\circ}C$ for 60 min. The LAMP product was visualized directly in one reaction tube by adding SYBR Green I. The detection limit of the LAMP assay was $10^{-2}J2/0.5g$ of soil, which was 10 times more sensitive than conventional PCR ($10^{-1}J2/0.5g$ of soil). Examination of 24 field soil samples revealed that the LAMP assay was applicable to a range of soils infested naturally with T. semipenetrans, and the total assay time was less than 2.5 h. These results indicated that the developed LAMP assay is a simple, rapid, sensitive, specific and accurate technique for detection of T. semipenetrans in field soil, and contributes to the effective management of citrus slow decline disease.
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Zhi-Qiang Song,Ju-E Cheng,Fei-Xue Cheng,De-Yong Zhang,Yong Liu 한국식물병리학회 2017 Plant Pathology Journal Vol.33 No.2
Tylenchulus semipenetrans is an important and widespreadplant-parasitic nematode of citrus worldwideand can cause citrus slow decline disease leading tosignificant reduction in tree growth and yield. Rapidand accurate detection of T. semipenetrans in soil isimportant for the disease forecasting and management. In this study, a loop-mediated isothermal amplification(LAMP) assay was developed to detect T. semipenetrans using DNA extracted from soil. A setof five primers was designed from the internal transcribedspacer region (ITS1) of rDNA, and was highlyspecific to T. semipenetrans. The LAMP reaction wasperformed at 63°C for 60 min. The LAMP productwas visualized directly in one reaction tube by addingSYBR Green I. The detection limit of the LAMP assaywas 10–2 J2/0.5 g of soil, which was 10 times moresensitive than conventional PCR (10–1 J2/0.5 g of soil). Examination of 24 field soil samples revealed that theLAMP assay was applicable to a range of soils infestednaturally with T. semipenetrans, and the total assaytime was less than 2.5 h. These results indicated thatthe developed LAMP assay is a simple, rapid, sensitive,specific and accurate technique for detection of T. semipenetrans in field soil, and contributes to the effectivemanagement of citrus slow decline disease.
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Song, Wen-Jie,Jiang, Ping,Cai, Jian-Ping,Zheng, Zhi-Qiang Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.15
Background: Cancers have dysfunctional redox regulation resulting in production of reactive oxygen species (ROS), damaging DNA, RNA and free NTPs, and causing the accumulation of oxidative nucleic acids in cytoplasm. The major types are 8-oxo-7,8-dihydroguanine(8-oxoGsn) in RNA and 8-oxo-7,8-dihydro-2' deoxyguanosine(8-oxodGsn) in Mt-DNA. The MTH1 protein sanitizes oxidized nucleotide pools from NTPs to monophosphates, preventing the occurrence of transversion mutations. This study concerned cytoplasmic 8-oxodGsn/Gsn and MTH1 expression in gastric cancer and para-cancer tissues and elucidated roles of nucleic-acid oxidation and anti-oxidation. Materials and Methods: A polymer HRP detection system was used to detect 8-oxo-Gsn/dGsn and MTH1 expression in 51 gastric cancer and para-cancer tissue samples. Analyses of patient clinical and pathological data were also performed. Results: The expression of MTH1 and the 8-oxo-dGsn/Gsn ratio were significantly higher in cancer tissues than para-cancer tissues (P<0.05). Cytoplasmic 8-oxo-Gsn and MTH1 were both found to positively correlate (P<0.05) with tumor differentiation, while no significant associations were found with gender, age, invasion depth, lymph node metastasis and clinical stage (P>0.05). Conclusions: We found 8-oxo-dGsn/Gsn and MTH1 are both highly expressed in gastric cancer tissues, especially in well differentiated lesions. In addition, oxidated mtDNA is prevalently expressed in gastric cancers, while 8-oxo-Gsn expression in cytoplasmic RNA is a bit lower, but more selectively.
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Zhong, Zhi-Qiang,Song, Mao-Min,He, Ying,Cheng, Shi,Yuan, Hui-Sheng Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.8
Background: Pancreatic cancer is one of the most aggressive tumors with a dismal prognosis. The membrane cytoskeletal crosslinker Ezrin participates in several functions including cell proliferation, adhesion, motility and survival. There is increasing evidence that Ezrin is overexpressed in vast majority of malignant tumors and regulates tumor progression. However, its roles in pancreatic cancer remain elusive. Methods: Three pairs of specific Ezrin siRNAs were designed and synthetized and screened to determine the most efficient one for construction of a hairpin RNA plasmid targeting Ezrin. After transfection into the Panc-1 pancreatic cancer cell line, real-time quantitative PCR and Western blotting were performed to examine the expression of mRNA and protein. The MTT method was applied to examine the proliferation and the drug sensibility to Gemcitabine. Flow cytometry was used to assess the cycle and apoptosis, while capacity for invasion was determined with transwell chambers. Furthermore, we detected phosphorylated-Erk1/2 protein and phosphorylated-Akt protein by Western blotting. Results: Real-time quantitative PCR and Western blotting revealed that Ezrin expression was notably down-regulated at both mRNA and protein levels by RNA interference (P< 0.01). Proliferation was inhibited and drug resistance to gemcitabine was improved (P< 0.05). Flow cytometry showed that the proportion of cells in the G1/G0 phase increased (P< 0.01), and in G2/M and S phases decreased (P< 0.05), with no apparent differences in apoptosis (P> 0.05). The capacity for invasion was markedly reduced (P< 0.01). In addition, down-regulating Ezrin expression had no effect on phosphorylated-Akt protein (P>0.05), but could decrease the level of phosphorylated-Erk1/2 protein (P< 0.05). Conclusions: RNA interference of Ezrin could inhibit its expression in the pancreatic cancer cells line Panc-1, leading to a potent suppression of malignant behavior in vitro. Assessment of potential as a target for pancreatic cancer treatment is clearly warranted.
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Zhang, Zhi-Qiang,Kim, Young-Min,Song, Soo-Chang American Chemical Society 2019 ACS APPLIED MATERIALS & INTERFACES Vol.11 No.38
<P>Intravenous (IV) route is the most commonly used drug-delivery approach. However, the targeting efficiency to tumor through IV delivery is usually less than 10%. To address this limitation, we report a new systemic delivery method utilizing injectable and quadruple-functional hydrogels to improve targeting efficiency through passive, active, and magnetic targeting, and hydrogel-controlled sustained release. The hydrogels consist of a folate/polyethylenimine-conjugated poly(organophosphazene) polymer, which encapsulates small interfering RNA (siRNA) and Au-Fe<SUB>3</SUB>O<SUB>4</SUB> nanoparticles to form a nanocapsule (NC) structure by a simple mixing. The hydrogels are localized as a long-term “drug-release depot” after a single subcutaneous injection and sol-gel phase transition. NCs released from the hydrogels enter the circulatory systems and then target the tumor through enhanced permeability and retention/folate/magnetism triple-targeting, over the course of circulation, itself prolonged by the controlled release. In vivo experiments show that 12% of NCs are successfully delivered to the tumor, which is a considerable improvement compared to most results through IV delivery. The sustained targeting of gold to tumor enables two cycles of photothermal therapy, resulting in an enhanced silencing effect of siRNA and considerable reduction of tumor volume, which we are unable to achieve via simple intravenous injection.</P> [FIG OMISSION]</BR>
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Xiao-Qiang Li,Gui-Qiang Guo,Hong-Zhi Gao,Nan Song,Dong-Sheng Li 한국정밀공학회 2015 International Journal of Precision Engineering and Vol. No.
Al-Cu-Mg aluminum alloy sheet is widely used for aircraft skin. Multi-stage forming combined with Solution Heat Treatment (SHT) is necessary for some geometrically complex productions in aircraft skin components. However, the effects of pre-strain and natural ageing time on the formability of Al-Cu-Mg aluminum alloy sheet are not fully understood. In this study, the deformation responses of an Al-Cu-Mg aluminum alloy sheet, over a range of pre-strain levels (0% to 6%) and natural ageing time levels (0 h to 2 h) were investigated. A modified Hollomon constitutive model coupling with pre-strain and natural ageing time was proposed to characterize the deformation responses of the Al-Cu-Mg aluminum alloy sheet. The hemispherical dome tests were performed at natural ageing time levels ranging from 0.25 h to 1.5 h. Marciniak and Kuczynski (M-K) theory was used to obtain the forming limit prediction model which takes pre-strain and natural ageing time into account. The theoretical Forming Limit Curves (FLCs) were compared with the experimental results.
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Wang, Zhi-Jun,Wang, Mao-Qiang,Duan, Feng,Song, Peng,Liu, Feng-Yong,Wang, Yan,Yan, Jie-Yu,Li, Kai,Yuan, Kai Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.3
Objective: This work aimed to evaluate the safety and clinical efficacy of transcatheter arterial chemoembolization (TACE) combined with c-arm cone-beam CT guided synchronous radiofrequency ablation (RFA) in treatment of large hepatocellular carcinoma (HCC). Methods: 21 patients with large HCC were studied from January 2010 to March 2012. TACE combined with synchronous C-arm cone-beam CT guided RFA were performed on a total of 25 lesions. Conventional imaging examination (CEUS, enhanced CT or MRI) and AFP detection were regularly conducted to evaluate the technical success rate of combined treatment, complications, treatment response, time without disease recurrence and survival rate. Results: The technical success rate of combined treatment was 100%, without any significant complication. After 1 month, there were 19 cases with complete response and 2 cases with partial response, with an complete response rate of 90.4% (19/21) and a clinical effective rate of 100% (21/21). The complete response rates of single nodular lesions (100%, 17/17) was significantly higher than that of multiple nodular lesions (50%, 2/4) (P<0. 05). During 2 to 28 months of follow-up, in 19 cases with complete response, the average time without disease recurrence was $10.8{\pm}6$ months. The total survival rates of 6, 12 and 18 months in 21 patients were 100%, respectively. Conclusion: TACE combined with synchronous C-arm CT guided RFA is safe and effective for treatment of large HCC. The treatment efficacy for single nodular lesion is better than that for multiple nodular lesions.
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Chen, Shu-Dong,Song, Mao-Min,Zhong, Zhi-Qiang,Li, Na,Wang, Pi-Lin,Cheng, Shi,Bai, Ri-Xing,Yuan, Hui-Sheng Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.3
Radixin, encoded by a gene on chromosome 11, plays important roles in cell motility, invasion and tumor progression. However, its function in pancreatic cancer remains elusive. In this study, radixin gene expression was suppressed with a lentivirus-mediated short-hairpin RNA (shRNA) method. We found that radixin shRNA caused down-regulation of radixin in PANC-1 cells, associated with inhibition of pancreatic cancer cell proliferation, survival, adhesion and invasive potential in vitro. When radixin-silenced cells were implanted in nude mice, tumor growth and microvessel density were significantly inhibited as compared to blank control cells or nonsense shRNA control cells. Thrombospondin-1 (TSP-1) and E-cadherin were up-regulated in radixin-silenced PANC-1 cells. Our results suggest that radixin might play a critical role in pancreatic cancer progression, possibly through invvolvement of down-regulation of TSP-1 and E-cadherin expression.
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Shuai Huang,Xiumin Xie,Qiang Xu,Xinhua Zhao,Guangwei Deng,Qiang Zhou,You Wang,Hai-Zhi Song 한국광학회 2020 Current Optics and Photonics Vol.4 No.6
A practical single photon source for fiber-based quantum information processing is still lacking. As a possible 1.55-µm quantum-dot single photon source, an InGaAsP/InP-air-aperture micropillar cavity is investigated in terms of fabrication tolerance. By properly modeling the processing uncertainty in layer thickness, layer diameter, surface roughness and the cavity shape distortion, the fabrication imperfection effects on the cavity quality are simulated using a finite-difference time-domain method. It turns out that, the cavity quality is not significantly changing with the processing precision, indicating the robustness against the imperfection of the fabrication processing. Under thickness error of ±2 nm, diameter uncertainty of ±2%, surface roughness of ±2.5 nm, and sidewall inclination of 0.5°, which are all readily available in current material and device fabrication techniques, the cavity quality remains good enough to form highly efficient and coherent 1.55-µm single photon sources. It is thus implied that a quantum dot contained InGaAsP/InP-air-aperture micropillar cavity is prospectively a practical candidate for single photon sources applied in a fiber-based quantum information network.
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