Partial Sequence Analysis of Puumala Virus M Segment from Bats in Korea

Yun, Bo-Kyoung,Yoon, Jeong-Joong,Lee, Yun-Tai The Korean Society of Virology 1999 Journal of Bacteriology and Virology Vol.29 No.1

Hantavirus is a genus of the Bunyaviridae family causing two serious diseases, hemorrhagic fever with renal syndrome (HFRS) and hantavirus pulmonary syndrome (HPS). Puumala virus is a member of hantavirus originally found in Europe, and its natural reservoir is Clethrionomys glareolus. It is also associated with the human disease nephropathia epidemica, a milder form of HFRS. To identify the hantaviruses in bats, bats were collected from Jeong-Sun, Won-Joo, Chung-Ju and Hwa-Cheon area in Korea, and nested RT-PCR was performed with serotype specific primer from M segment. Interestingly, Puumala virus was detected in bats (Rhinolophus ferrum-equinum) only from Won-Joo. The 327 bp nested RT-PCR product, was sequenced. The sequence database search indicates that the sequence is homologous to the published sequence of Puumala viruses. The sequence similarities were ranged from 71% to 97%. The highest sequence similarity was 97% with Puumala virus Vranicam strain, and the lowest was 71% with Puumala virus K27 isolate. Puumala virus Vranicam strain was isolated from a bank vole (Clethrionomys glareolus) in Bosnia-Hercegovina. Puumala virus K27 was isolated from human in Russia. This analysis confirms that bats (Rhinolophus ferrum-equinum) in Korea are natural reservoir of Puumala virus.

Detection of Puumala and Hantaan Virus among Bats in Korea by Nested RT - PCR

Yun Tai Lee,Bo Kyoung Yun,Jeong Joong Yoon 대한바이러스학회 1998 Journal of Bacteriology and Virology Vol.28 No.2

Hantavirus is a genus of the Bunyaviridae family consisting following serotype groups: Hantaan, Seoul, Puumala, Prospect Hill, Thailand, Belgrade, Thotta palayam, Sin Nombre. Most of Hantavirus group have been associated with many clinically similar disease known collectively as hemorrhagic fever with renal syndrome (HFRS). Hantaan virus is the prototype of the genus hantavirus, originally isolated from Apodemus agrarius. Bat was found as a natural host for Hantaan virus in Lee's lab for the first time. Then, Hantaan-like virus was isolated Hantaan-like virus from bat. To identify hantaviruses that are present in Korea among bats, bats were collected from Jeong-Sun, Won-Joo, Chung-Ju and Hwa-Cheon area, RNA was isolated from lung and serum. RT-PCR was performed with a universal primer from M segment. Nested RT- PCR was carried out to differentiate Hantaan, Seoul and Puumala virus using serotype specific primers. As we expected, Hantaan viruses were detected in bats and Seoul virus was not detected. Interestingly, Puumala viruses were also detected in bats from Won-Ju, but not in other areas. Puumala virus is originally isolated from Clethrinomys glareolus, and cause light HFRS. Recently, Paradoxomis webbiana, a wild bird turn out to be a reservoir for Puumala virus in Korea. These data indicate that bat is a new natural reservoir of Puumala virus.

Detection of Puumala and Hantaan Viruses among Bats in Korea by Nested RT-PCR

Lee, Yun-Tai,Yun, Bo-Kyoung,Yoon, Jeong-Joong The Korean Society of Virology 1998 Journal of Bacteriology and Virology Vol.28 No.2

Hantavirus is a genus of the Bunyaviridae family consisting following serotype groups: Hantaan, Seoul, Puumala, Prospect Hill, Thailand, Belgrade, Thotta palayam, Sin Nombre. Most of Hantavirus group have been associated with many clinically similar disease known collectively as hemorrhagic fever with renal syndrome (HFRS). Hantaan virus is the prototype of the genus hantavirus, originally isolated from Apodemus agrarius. Bat was found as a natural host for Hantaan virus in Lee's lab for the first time. Then, Hantaan-like virus was isolated Hantaan-like virus from bat. To identify hantaviruses that are present in Korea among bats, bats were collected from Jeong-Sun, Won-Joo, Chung-Ju and Hwa-Cheon area, RNA was isolated from lung and serum. RT-PCR was performed with a universal primer from M segment. Nested RT-PCR was carried out to differentiate Hantaan, Seoul and Puumala virus using serotype specific primers. As we expected, Hantaan viruses were detected in bats and Seoul virus was not detected. Interestingly, Puumala viruses were also detected in bats from Won-Ju, but not in other areas. Puumala virus is originally isolated from Clethrinomys glareolus, and cause light HFRS. Recently, Paradoxomis webbiana, a wild bird turn out to be a reservoir for Puumala virus in Korea. These data indicate that bat is a new natural reservoir of Puumala virus.

고등학생의 진로결정에 관한 구조방정식모형의 구축

정윤경 한국직업능력개발원 2002 직업능력개발연구 Vol.5 No.2

The purpose of this study was 1) to identify the high school students' career decision related variables, and 2) to construct a structural equation model on high school students' career decision making, and 3) to examine fit of a structural equation model on high school students' career decision making using LISREL. The sample of this study was Jeong(2000)'s raw data which included 1,165 high school students. The results demonstrated the following points : 1) This study find evidence of hypothesis 1 through hypothesis 11 except hypothesis 10. 2) There were significant direct effects of career informaion on career decision level. 3) There were significant direct effects of self confident level on career decision level. 4) There were significant direct effects of anxiety about self career on career decision level. 5) There were not significant direct effects of insecurity about career decision making on career decion level. 6) There were significant direct effects of career decision level on carer preparation behavior level. Based on the results of this study, He findings suggest that 1) Researchers/practitioners in career guidance institutes should concentrate on developing career informations, and 2) they should try to deliver career informations to high school students. 3) Career guidance program should support high school students to develop self confidence on career decision and to cope with anxiety about self career. Also the findings suggest that 4) Ministry of education and human resources development should support public administrative, and financial needs of career guidance for high school students.

반복성 단늑골 다지 증후군 1예

방성윤,최규연,박은희,강미경,김미경,이정재,이임순 순천향의학연구소 2005 Journal of Soonchunhyang Medical Science Vol.11 No.1

Short-rib polydactyly syndromes(SRPS) comprise a group of rare, generally lethal skeletal dysplasias. This group is manifested by short-limb short stature, short ribs with thorasic hypoplasia, and polydactyly. This heterogenous group of recessively inherited disorder has distinct imaging findings and ancillary findings on both pre-and postnatal assessments may enable individual cases to be classified into one of four subtypes : SRPS Ⅰ(Saldino-Noonan); SRPS Ⅱ (Majewski); SRPS Ⅲ (Verma-Naumoff); and SRPS Ⅳ (Beemer-Langer)1,2,3). All forms of the SRPS described to date are thought to be inherited in an autosomal recessive manner. There are difficulties in the clssification of these disorders because of the frequent overlap. We experienced one case of recurrent short-rib polydactyly syndrome and presented with review of literature.

Quartz 자극에 의한 iNOS 발현의 자가조절

김경아,남혜윤,문제혁,정진숙,임영,정치경 大韓産業醫學會 2001 대한직업환경의학회지 Vol.13 No.4

목적 : Rat2 섬유모세포에서 α-quartz에 의해 유도된 NOS의 조절기전을 알아보기 위하여 본 연구가시도 되었다. 방법 : Rat2 세포에 있어서 α-quartz 자극에 의한 NO와 H₂O₂의 분비 및 iNOS의 발현을 관찰하였고, iNOS 억제제인 L-NIL과 H₂O₂ 억제제인Catalase를 전처리하고 마찬가지로 NO와 H₂O₂분비 그리고 iNOS의 단백발현 정도를 western blot-ting을 이용하여 관찰하였다. 결과 : o-quartz는 Rat2 세포에서 iNOS에 의한NO와 H₂O₂의 분비를 유도하였다. L-NIL은 R3t2세포에서 D-quartz에 의한 H₂O₂의 분비와 iNOS발현을 완전히 억제하였다. Catalase로 전처리 하였을 때 o-quartz로 유도된 iNOS에 의하여 생산증가된 H2Oa가 iNOS를 억제하는 autoinhibitorypathway가 차단되어 결과적으로 H₂O₂의 분비와 iNOS의 발현이 증가하였다. 결론 : Rat2 세포에서 α-quartz에 의하여 유도된iNOS는 H₂O₂의 분비를 촉진하고 이 H₂O₂는 iNOS기능을 조절하는 자가조절기전에 중요한 역할을 하는 것으로 생각된다. Objectives : This study was performed in order to investigate the molecular mechanism regulating nitric oxide synthase (NOS) induced by α-quartz in Rat2 fibroblast. Methods : α-quartz-induced nitric Oxide (NO) anti H₂O₂ formation anti α-quarts-induced iNOS protein expression in Rat2 fibroblast were monitored. With iNOS inhibitor (L-N6- (1-iminoethyl) lysine hydrochloride, L-NIL) or antioxidant (catalase), we observed NO and H₂O₂ formation and iNOS protein expression in Rat2 fibroblast stimulated with α-quartz. Results : α-quartz stimulated iNOS-induced NO and H₂O₂ formation in Rat2 fibroblast. L-NIL inhibited H₂O₂ formation and iNOS protein expression by α-quartz in Rat2 fibroblast. Pretreatment with catalase blocked the autoinhibitory pathway of iNOS by iNOS-induced H₂O₂ therefore H₂O₂ and NO production and iNOS protein expression were increased in Rat2 fibrobalst stimulated with α-quartz Conclusion : α-quartz-induced iNOS Stimulated H₂O₂ formation in Rat2 fibroblast. INOS-induced H₂O₂ by α-quartz plays an important role in the autoinhibition pathway for regulating the iNOS function in Rat2 fibroblast

일부 산업장근로자들의 피로자각증상과 일상생활요인과의 관련성

김정윤,조영채,이동배,박내경 大韓産業醫學會 1998 대한직업환경의학회지 Vol.10 No.2

For the purpose of obtaining reference materials for the prevention and management of mental health promoting in industrial workers, this survey was investigated the relationship between subjective fatigue symptoms and its related factors such as demographic, job and health related variables. 442 cases of industrial workers which occurred in 7 factories of machine and metal manufacturing industries in Taejon industries area surveyed by self-recorded questionnaire. The results were as following: 1. In the complaint rates of fatigue, "eye strain" was the highest(21.9%) and followed by "feel like lying"(12.4%) and "feel a pain in the low back"(12.4%), "feel drowsy"(12.2%), "yawning a lot"(11.8%) and "whole body feels tired"(11.1%) in the descending order. 2. In the average weighted scores of fatigue complaints, dullness and sleepness group(Ⅰ) was the highest, followed by difficulty in concentration group(Ⅱ) and bodily projection of fatigue group(Ⅲ) in the descending order. 3. The average weighted scores of fatigue complaints by general characteristics were significantly higher in the lower age group, lower education group and unmarried divorce group. But there was no significant difference in sex. 4. By the working condition, the fatigue scores were significantly higher in manual workers and shift worker than in clerical worker and day worker. 5. By the life style, the fatigue scores were significantly lower in 7-8 sleeping hour group and every day eating breakfast group than in other groups. But fatigue score of everyday alcohol drinking group and the lower health practice indecies group were significantly higher than that of other groups. 6. By the health status and psychological factors, the fatigue scores were higher in unhealthy group, unsatisfaction income level group, unsatisfaction worker contents group and the group of badly self-control in work. 7. In the stepwised multiple regression, factors affecting the fatigue symptoms scores were depression symptom score, health status, marital status, job satisfaction, job repeatedness and body mass index.

최대/최소 경계 스키마 추출 기법을 이용한 XML 문서의 DTD 추출

박경현,최은선,이종연,박정석,류근호 충북대학교 컴퓨터정보통신 연구소 2000 컴퓨터정보통신연구 Vol.8 No.2

XML이 인터넷상의 데이터 교환의 표준으로 대두되면서 데이터 모델이나 플랫폼에 관계없이 데이터의 전송이 가능하게 되었다. 특히 데이터 중심의 XML 문서의 경우 전송시의 부하를 줄이기 위해 DTD없이 전송되는 경우가 일반적이다. 그러한 이유로 전송받은 XML 데이터의 저장 효율을 높이고 질의를 최적화하며 사용자에게 편의성을 제공해주기 위해서는 XML 데이터로부터 DTD 추출이 필수적으로 요구된다. 이 논문에서는 이러한 DTD를 추출하기 위해 반구조적 데이터의 스키마 추출 기법을 적용한다. 특히, 반구조적 데이터의 최소 경계 스키마를 추출하는데 데이터로그를 이용하는 기존의 방법보다 효율적인 방법인 시뮬레이션을 적용함으로써 보다 향상된 DTD 추출 기법을 제시한다. XML makes it possible for data to be exchanged regardless of the data model in which it is represented or the platform on which it is stored, serving as a standard for data exchange format on the internet. Especially, It is natural to send XML data without DTD on the internet when XML is data-centric XML. Therefore it is needed to extract DTD to store XML data efficiently, to optimize queries, and to help users browse XML data. In this paper, Techniques for upper/lower bound schema extraction are applied to extract DTD from XML data. Especially we propose an efficient technique for extracting DTD by using simulation which is better than datalog as a lower bound schema extraction method.

새로운 백금착체인 tetrachloro [bis(2-chloroethyl)ethylenediamine-N,N']platinum(IV)과 cisplatin이 마우스의 면역반응에 미치는 영향

표명윤,유경미,최지선,오현정 숙명여자대학교 약학연구소 1993 약학논문집-숙명여자대학교 Vol.9 No.-

Effects of a new platinum complex, tetrachloro-[bis(2-chloroethyl) ethylenediamine-N,N']platinum(IV)[PtCl₄-2(CEen)] and cisplatin on the immune response were investigated. Male ICR mice were treated at the respective LD50(20% or 60% LD50) of cisplatin and PtCl₄-2(CEen). The results were as following : LD50 of PtCl₄-2(CEen) for male ICR mouse was 122.5mg/kg(i.p.) and higher than cisplatin(16.3mg/kg, i.p.). Body-, spleen-, thymus-, liver-weight, number of WBC and RBC were reduced dependently on the dose and day of administeration of cisplatin and PtCl₄-2(CEen). But these toxicities produced by PtC^-^CEen) appeared to be relatively less and to improve faster than by cisplatin. The titers of hemagglutinin and hemolysin to SRBC in mice received cisplatin and PtCl₄-2(CEen) were decreased significantly and independently on the day of antigen injection. Cisplatin and PtCl₄-2(CEen) stimulated significantly contact hypersensitivity to DNFB when administered prior to sensitization(DNFB), but suppressed slightly when administered after sensitization.

혼합형 자가면역성용혈성빈혈과 동반된 Evans 증후군 1례

선길홍,윤찬영,박상곤,박경희,우정주,한경택,김진화,김영훈,정춘해,박치영 朝鮮大學校 附設 醫學硏究所 2005 The Medical Journal of Chosun University Vol.30 No.2

Evans syndrome is defined as a simultaneous or sequential occurrence of autoimmune hemolytic anemia and autoimmune thrombocytopenia. Autoimmune hemolytic anemia (AIHA) is usually induced by IgG warm autoantibody or cold-active IgM antibodies reacting specifically with antigens associated with a patient's RBC. AIHA is a fairly uncommon disorder, with estimates of the incidence at 1 to 3 cases per 100,000 per year. Mixed-type AIHA is a relatively uncommon form of AIHA, with studies noting the incidence of 7-8% among cases of AIHA. We experienced a patient, 46-year-old woman who was diagnosed having a very rare clinical presentation of mixed warm and cold antibody mediated Evans syndrome. She was treated with corticosteroid therapy only and has been maintaining a complete response for 15 weeks. 저자들은 혼합형의 자가면역성 용혈성 빈혈과 자가면역성 혈소판 감소증이 동시에 발생한 혼합형 Evans 증후군으로 진단하고 스테로이드 요법 후 혈액학적으로 회복된 상태로 15주가 지난 현재 steroid 5 mg/일 까지 감량한 상태에서 추적 관찰 치료중인 46세 여자 환자를 경험하였기에 문헌 고찰과 함께 보고하는 바이다.