한국인 주요우울장애 환자에서 혈중 ACTH, Cortisol 농도와 해밀턴 우울 평가 척도의 신체증상 항목과의 상관관계

여혜빈,김린,함병주,심세훈,권영준,정희연,정한용,한상우,이민수,이화영 대한생물치료정신의학회 2011 생물치료정신의학 Vol.17 No.2

Objectives:Hypothalamic-Pituitary-Adrenocortical(HPA) system dysregulation plays an important role in the pathophysiology of major depressive disorder. Medically unexplained somatic symptoms comprised the predominant complaints of korean patients with major depressive disorder. It might be related to dysregulation of HPA system and somatic symptoms. The aim of this study was to investigate the association between Hamilton Depression Rating Scale severity and the Plasma Adrenocorticotropic Hormone(ACTH) & Serum Cortisol level in Korean patients with major depressive disorder. Methods:Our study design was prospective. A total 111 hospitalized patients with major depressive disorder (DSM-IV) at Anam Hospital, Korea University Medical Center between Jan 2009 and May 2011 were selected. Hamilton Depression Rating Scale was measured by psychiatrist. Plasma ACTH, Serum Cortisol levels were analyzed at the time of admission. Results:There are significant correlations(p<0.05) between Hamilton Depression Rating Scale of somatic component(HAM-D 11-15 item) and Plasma ACTH and Serum Cortisol level. However, other HAM-D sub items were not significantly correlated with Plasma ACTH and Serum Cortisol level except somatic component. Conclusion:Our Study results suggest that hyperactivity of HPA axis might be correlation with somatic symptoms in korean patients with major depressive disorder.

다공성 충전층반응기에서 기-액흐름양상의 전이와 액체체류량

손민일,함병호,조지훈,김태옥,장영준 明知大學校 産業技術硏究所 1996 産業技術硏究所論文集 Vol.15 No.-

For both downward and ward cement gas-liquid flow in a porous packed bed reactor the transition of gas-liquid flow pattern and liquid holdup were investigated experimentally in an air-water-Zeolite particle system. Experimental results showed that gas velocity at trickle-to-pulse flow transition boundary in the downflow system was decreased with increasing liquid velocity and that gas velocity at bubble-to-pulse flow transition boundary in the upflow system was nearly constant for lower liquid velocity, however, it was increased with increasing liquid velocity for turbulent flow region. We also found that the dynamic liquid holdup in the upflow system was higher than that in the downflow system and it was increased with increasing liquid velocity and with decreasing gas velocity. Furthermore,the dynamic liquid holdup was sensitively affected by liquid velocity at trickle flow in the downflow system while it was sensitively affected by gas velocity at bubble flow in the upflow system and at pulse flow in the downflow and upflow systems. For the porous particle system fluid velocities at gas-liquid flow transition boundaries and dynamic liquid holdup had nearly same that for the nonporous particle(glass bead) system.

Bacillus 속 분리주가 생산하는 박테리오신의 특성 조사

함승희(Seung-Hee Ham),최낙식(Nack-Shick Choi),문자영(Ja-Young Moon),백선화(Sun-Hwa Baek),이송민(Song-Min Lee),강대욱(Dae-Ook Kang) 한국생명과학회 2017 생명과학회지 Vol.27 No.2

토하젓에서 분리한 박테리오신 생산 균주 중 상대적으로 넓은 항균스펙트럼을 나타내는 1균주를 선발하였고 16S rRNA 유전자 염기서열 분석 결과 B. subtilis E9-1와 거의 일치하는 것으로 동정되었다. B. subtilis E9-1가 생산하는 박테리오신의 물리화학적 특성을 조사하였고, 박테리오신을 정제하였다. 이 박테리오신은 B. cereus KCCM 11204, M. luteus IAM 1056, L. monocytogenes KCCM 40307, E. faecium KCCM 12118 및 S. aureus subsp. aureus KCCM 40050 등에 대해서 항균활성을 나타내었다. pH 2.0~8.0 범위에서는 안정하였으나 8.0 이상에서는 항균활성이 감소하였다. Isopropanol, ethanol 및 methanol 등의 유기용매에서 100%까지, acetone과 acetonitrile에서는 80%까지 항균활성을 유지하였다. 내열성의 경우 40~100℃에서 60분까지는 안정하게 항균활성을 보였다. 박테리오신 농도를 증가시키면서 B. cereus, L. monocytogenes, E. faecium 및 S. aureus subsp. aureus 등 시험균 4주의 감수성을 조사한 결과 농도 의존적으로 시험균의 생육이 감소하였고 이중 L. monocytogenes 의 감수성이 가장 높게 나타났다. 박테리오신의 작용양상을 알아본 결과 B. cereus와 L. monocytogenes 배양액에 박테리오신 용액을 첨가한 후 흡광도와 CFU값이 감소하여 bactericidal임을 확인하였다. 식품에 적용 가능성을 알아보기 위해 실험한 결과 3일째부터 박테리오신을 처리하지 않은 대조구에 비해 실험구에서 생균수(CFU/ml)가 감소하였다. 아세톤을 이용한 배양상등액의 농축, superdex peptide HR 10/300 column 이용한 겔여과크로마토그래피, 역상 HPLC로써 박테리오신을 정제하였다. 역상 HPLC를 통해서 정제한 박테리오신의 분자량을 tricine SDS- PAGE로 분석한 결과 약 4 kDa이었고 질량분석법으로 측정한 정확한 분자량은 3347.6 Da로 나타났다. As an effort to find a potential biopreservative, we isolated bacterial strains producing bacteriocin from fermented foods. A strain was finally selected and characteristics of the bacteriocin were investigated. The selected strain was identified as Bacillus subtilis E9-1 based on the 16S rRNA gene analysis. The culture supernatant of B. subtilis E9-1 showed antimicrobial activity against Gram-positive bacteria. Subtilisin A, α-chymotrypsin, trypsin and proteinase K inactivated the antimicrobial activity, which means its proteinaceous nature, a bacteriocin. The bacteriocin activity was fully retained at the pH range from 2.0 to 8.0 and stable at up to 100℃ for 60 min. Solvents such as ethanol, isopropanol and methanol had no effect on the antimicrobial activity at the concentration of 100% but acetone and acetonitrile reduced the activity at up to 100% concentration. Cell growth of four indicator strains was dramatically decreased in dose-dependent manner. Listeria monocytogenes was the most sensitive, but Enterococcus faecium was the most resistant. Bacillus cereus and Staphylococcus aureus showed the medium sensitivity. The bacteriocin showed its antimicrobial activity against B. cereus and L. monocytogenes via bactericidal action. The number of viable cells of L. monocytogenes started to reduce after addition of bacteriocin to the minced beef. The bacteriocin was purified through acetone concentration, gel filtration chromatography and RP-HPLC. The whole purification step led to a 6.82 fold increase in the specific activity and 6% yield of bacteriocin activity. The molecular weight of the purified bacteriocin was determined to be 3.3 kDa by MALDI-TOF/TOF mass spectrometry.

한국의 노인우울증 환자에서 조발성 우울증과 만발성 우울증의 임상증상의 비교

박기홍,이화영,함병주,이민수,Park, Ki-Hong,Lee, Hwa-Young,Ham, Byung-Joo,Lee, Min-Soo 대한생물정신의학회 2010 생물정신의학 Vol.17 No.3

Objectives : Clinical differences between elderly patients with early and late onset depression have been described although these have been inconsistent. We aimed to compare differences of clinical symptoms using the 17 items Hamilton Rating Scale for Depression(HAM-D-17) between two groups. Methods : Data of 175 elderly patients with a diagnosis of major depressive disorder according to DSM-IV from January 2005 to November 2009 were collected. Seventy five patients were early onset depression and one hundred patients were late onset depression. Depressive symptoms were assessed by the 17-item Hamilton Rating Scale for depression. Results : There were some differences in HAM-D-17 scores between early and late onset depression. Early onset depression patients scored significantly higher in retardation(t = 2.41, p = 0.017) and somatic symptoms( general)(t = 2.37, p = 0.019) than late onset depression patients. Conclusion : We concluded that early onset depression patients have more severe psychomotor retardation and general somatic symptoms than late onset depression patients in Korea. Because of some limitations of this study, further investigations will be needed to validate this study results.

구미지역 직업성질환 감시체계

김성아,김진석,전혜리,정상재,김상우,이채용,함정오,유재영,최태성,하봉구,조민환,우극현 大韓産業醫學會 2003 대한직업환경의학회지 Vol.15 No.1

목적: 구미지역 내 대학병원이자 특수건강진단·보건관리대행기관의 산업의학전문의, 보건관리대행 간호사, 사업장 간호사를 중심으로 직업성질환감시체계를 구축하여 주요 직업성질환들의 발생현황과 규모를 파악하고자 하였다. 또한 연차적으로 산업, 직업, 업무내용 등에 따른 분포나 원인을 기존 자료원(특수건강진단의 직업병 유소견자와 산재요양승인 되는 직업병자)과 비교하여 분석·기술하고 예방 및 관리를 포함한 보건의료기술개발까지을 포함하는 종합적인 감시체계를 구축하고 운용하고자 하였다. 방법: 구미지역의 산업적 특성에 맞는 직업성질환감시체계를 구축하고 지역 감시체계의 모델을 제시하였다. 6대 감시대상 직업성질환(독성 간염, 직업성천식, 직업성 근골격계질환, 직업성 피부질환, 직업성 암, 수지진동증후군)의 실행적 환례정의, 보고양식 및 보고체계 개발, 분석양식 및 결과 배포 형식을 개발하였으며, 그 유병규모를 파악하고, 감시질환의 분포 및 특성, 관련인자 등을 분석·기술하고자 하였다. 이때, 1년간의 환례분석시 직업병 유소견자 및 직업병 요양자에 대한 자료와 함께 비교 분석하였으며, 노동통계자료를 이용하여 년간 발생률을 추정하였다. 결과: 2001. 1. 1일부터 2002.4.30일까지 총 287건이 보고되었으며, 직업성 근골격계질환 132건(46.0%), 직업성 피부질환 100건(34.8%), 독성간염 34건 (11.8%), 직업성천식 16건 (5.6%), 수지진동증후군 5건 (1.7%) 이었으며 직업성 암은 한건도 보고되지 않았다. 보고경로별로는 보건관리대행에서 33건 (11.5%), 해당 유해인자별로 실시되는 특수검진 외의 각종 건강진단업무 수행 중 발견된 환례에 대한 보고가 206건(71.7%)이었다. 직종별 분류의 결과는 섬유업종사자, 단순노무종사자, 음식업종사자 등이 다수를 차지하였다. 근골격계질환의 경우, 수근관증후군이 42건(33%), 외상과염/내상과염이 39건(30%)로 가장 많았다. 직업성 피부질환은 97건이 접촉성 피부염으로 유기용제류가 59건(59%)으로 가장 많고, 니켈 및 에폭시수지에 의한 알레르기성 접촉성 피부염이 각각 6건과 5건이었다. 독성 간염은 모두 DMF 나 DMAc에 의한 것으로, 근무시작 후부터 간기능이상이 발견되기까지의 근무기간은 대부분이 3개월 이하이며, 1개월 이하인 경우도 10건이나 되었다. 동 기간 중 감시환례에 대해 산재요양 자료와 중복여부를 확인한 결과, 중복되는 것은 하나도 없었다. 특수건강진단에서 직업병유소견자로 판정된 경우는 독성간염에서 3건이 있었으며, 나머지 질환은 중복되는 것은 없었다. 2001도 구미지역의 근로자수와 보고환례건수를 이용하여 연간 발생률을 추정하였다. 근로자 10만 명당 연간 발생률은 상지 근골격계질환은 63.6명, 피부질환 48.2명, 독성 간염 17.9명, 천식 7.7명, 수진진동증후군 2.4명이었다. 참여예상인원은 총 46명이었으며, 실제 참여자수는 21명, 평균참여율은 46%, 실제 참여 1이당 평균 보고건수는 산업의학전문의가 28건으로 가장 많았고, 대행간호사는 45,건, 사업장 간호사는 3.3건이었다. 결론: 지역 감시체계는 기존 통계자료들에서 발견되지 않았던 직업성질환의 발견에 유용한 통계원이며, 특히 지역의 특성과 여건을 고려하여 체계화된 시스템을 구축하여 운용한다면 더 성과가 클 것이다. Objectives: Area-based occupational disease surveillance in Kumi (KODS) was used to collect data on occupational diseases. The data was used to estimate the magnitude of the diseases, to analyze for their characteristics and to find links for their intervention and prevention. Methods: Since January 2001, occupational physicians and nurses in Occupational Health Service (OHS) have reported six major occupational diseases; occuaptional musculoskeletal disorders of the upper extremities (MSDUE), occupational dermatoses, toxic hepatitis, occupational asthma (OA), hand-arm vibration syndrome (HAVS), and occupational cancer. For the respective diseases, a reporting sheet and operational diagnostic criteria were developed by the KODS. An analysis of the KODS data, collected over a certain period, was compared with data from the Korea Labor Workers Corporation (KLWC) and the Specific Health Examination (SHE), and the incidence rates of the diseases estimated. Results: Between Jan 2001 and Apr 2002, 287 cases of the six major occupational disease were reported. Of these, there were 132 (46.0%), 100 (34.8%), 34 (11.8%), 16 (5.6%), and 5 (1.7%), cases of MSDUE, occupational dermatoses, toxic hepatitis, OA, and HAVS, repectively. But, there were no cases of occupational cancer. 33 (11.5%) of the incidence were reported via the OHS, 206 (71.7%) from health checkups, and the other 48(16.8%) from other routes. The synthetic fibers and electronic components manufacturing industries accounted for the greatest number of reported cases. With resport to occupation, the greatest numbers of incidence were reported from the elementary occupations, textile workers, assemblers, and cooks and food services worker, in that order. Of the MSDUE cases, carpal tunnel syndrome and epicondylitis were the two most common diseases. Most of the occupational dermatoses were due to contact dermatitis, with organic solvents (59%), nickel (6%), and epoxy resin (5%) accounting for the majority of these cases. All cases of toxic hepatitis were induced by dimethylformamide or dimethylacetamide, which were reported by a unique monitoring system. During same period, there were no cases claimed by workers to the KLWC, and only three cases from SHE, were identified. Using data from workers' number and surveillance, the annual incidence of MSDUE, dermatoses, hepatitis, OA, and HAVS, per 100,000 workers were 63.6, 48.2, 17.9, 7.7, and 2.4, respectively. Conclusions: These results show that an area-based surveillance system can be very effective for the collection of data on work-related diseases, at least in Kumi.

건설현장 미세먼지 억제를 위한 물 입자 분사 드론 시스템 개발에 대한 연구

최영민(Young-Min Choi),함영복(Young-Bog Ham),박중호(Jung-Ho Park),김홍욱(Hong-Uk Kim) 한국산학기술학회 2021 한국산학기술학회논문지 Vol.22 No.10

본 논문은 소규모 건설현장을 포함하는 도심지 건설현장에 대한 미세먼지 저감 방안에 대하여 드론기반의 water fog 분사시스템에 관한 것이다. 직경 200 ㎛의 노즐을 사용하여 8 ~ 15 ㎫ 압력 조건에서 약 10 ㎛ 직경의 미분수 분포를 확인하였다. 시뮬레이션 해석결과, 1,000 RPM에서 노즐 토출압력 7.83 ㎫, 총 유량 1.03 ℓ를 나타내며 분사시간에 따라 점차적으로 감소하였고, 모터 회전속도에 비례하여 토출압력이 증가하였다. 한편 2000 RPM에서 노즐한 개당 유량은 1.42 ℓ로써 10 ℓ의 용량으로 10분간 분사가 가능한 것으로 나타났다. 실제, BLDC모터를 1,000 RPM으로 구동하여 한 개의 노즐에서 최대 토출압력 5.51 ㎫ 및 총 유량 0.69 ℓ를 나타냈다. 그리고 모터 회전속도에 비례하여 토출압력 및 유량이 증가하였으며, 2,500 RPM에서 노즐 한 개당 1.00 ℓ/min이상의 유량과 10 ㎫이상의 압력을 나타냈다. 따라서 드론 기반의 water fog 분사시스템 구축이 가능한 것으로 나타났다. In this paper, a drone-based water fog spraying system was investigated for its effectiveness in reducing fine dust at urban construction sites. A nozzle with a diameter of 200㎛ was used to determine the distribution of differential fractions with a diameter of about 10㎛ under a pressure of 8 to 15 ㎫. The result of the simulation analysis shows that the outlet pressure of the nozzle was 7.83 ㎫, and the total flow rate was 1.03 liter at 1,000 RPM. The outlet pressure was increased proportional to the rotation speed of the motor. The integrating flow rate of the nozzle was 1.42 liter at 2000 RPM. The BLDC motor was driven at 1,000 RPM, resulting in a maximum outlet pressure of 5.51 ㎫ and a flow rate of 0.69 liter/min at the nozzle. The outlet pressure and flow rate were increased with the rotational speed of the motor. The flow rate was more than 1.00 liter/min for the nozzle, and the outlet pressure of more than ten ㎫ at 2,500 RPM. Therefore, these results suggest that it is possible to establish a water fog spraying system using drones.

동적 신장 신티그래피 검사 시 사용되는 Gates 법과 Modified Gates 법의 사구체 여과율 비교에 관한 고찰

함준철,반영각,박민수,조석원,임한상,김재삼,이창호,Ham, Jun-Cheol,Bahn, Young-Kag,Park, Min-Soo,Cho, Seok-Won,Lim, Han-Sang,Kim, Jae-Sam,Lee, Chang-Ho 대한핵의학기술학회 2012 핵의학 기술 Vol.16 No.1

사구체 여과율은 신기능의 평가 및 신 질환의 조기 발견 및 만성 신 질환 환자의 경과 관찰에 중요한 지표가 된다. 본 연구에서는 동적 신장 신티그래피의 Gates법과 Modified Gates법을 채혈을 이용한 MDRD법 공식을 기준으로 그 차이를 비교, 분석하고자 한다. 2010년 11월부터 2011년 8월까지 본원에 내원하여 신장 신티그래피 검사를 시행한 45명의 환자를 대상으로 시행하였다. 이 중 20명의 환자는 AGUS(Philips Medical System, Cleveland, OH, USA) 장비의 Gates법과 MDRD법(Modification of Diet Renal Disease) 공식에 의한 사구체 여과율 값을 비교하였으며, 20명의 환자는 INFINIA (General Electric Healthcare, Wisconsin, MI, USA) 장비의 Modified Gates법과 MDRD법 공식에 의한 사구체 여과율 값을 비교하였다. 마지막으로 경과 관찰 시 MDRD법 공식에 의한 사구체 여과율 변화가 없는 환자 5명을 대상으로 Gates법과 Modified Gates법을 비교하였다. Gates법과 Modified Gates법 모두 MDRD법 공식에 의한 사구체 여과율 값과 높은 상관 관계를 보였으며($p$<0.01, r=0.903, r=0.867), Gates법의 대응 차 평균은 $2.05{\pm}2.54mL/min/1.73m^2$로 나타났고, Modified Gates법의 대응 차 평균은 $25.2{\pm}3.71mL/min/1.73m^2$로 나타났다. 마지막으로 5명의 환자에서 실시된 Gates법과 Modified Gates법의 비교는 높은 상관 관계를 보였으며($p$<0.05, r=0.949), 대응 차 평균은 $20.4{\pm}8.84mL/min/1.73m^2$ 로 나타났다. Gates법과 Modified Gates법 및 MDRD법 공식에 의한 사구체 여과율 값은 서로 높은 상관 관계를 보였다. 동적 신장 신티그래피 검사 시 사용되는 Gates법과 Modified Gates법의 상관 관계를 인지하고 검사를 진행 한다면, 진단능을 향상시켜 정확한 신기능의 평가가 이루어질 것으로 사료된다. Purpose: Glomerular filtration rate is an important index for assessment of renal function, early discovery of renal disease, and progress observation of chronic renal disease patients. In the present study, the objective is to conduct a comparative analysis of differences between Gates and Modified Gates method in dynamic renal scintigraphy based on MDRD (Modification of Diet Renal Disease) formula using blood collection. Materials and Methods: Renal scintigraphy was performed for 45 patients who visited our hospital between November 2010 and August 2011. For 20 patients of those tested, glomerular filtration rates from Gates method and MDRD formula using AGUS equipment, were compared. For the other 20 patients, glomerular filtration rates from Modified Gates method and MDRD formula using INFINIA equipment. Finally, Gates and Modified Gates method were compared for 5 patients who indicated no change in glomerular filtration rates from MDRD formula during progress observation. Results: Glomerular filtration rates from both Gates and Modified Gates method showed a high correlation with those from MDRD formula ($p$<0.01, r=0.903, r=0.867), with a paired difference mean for Gates method of $2.05{\pm}2.54mL/min/1.73m$, and that for Modified Gates method of $25.2{\pm}3.71mL/min/1.73m$. Finally, the values for Gates method and those for Modified Gates method showed a high correlation for the five patients ($p$<0.05, r=0.949), with a paired difference mean of $20.4{\pm}8.84mL/min/1.73m$. Conclusion: Glomerular filtration rates from Gates method, Modified Gates method and MDRD formula showed mutually high correlations. If the tests are performed with recognition for the correlations between Gates and Modified Gates method used in a dynamic renal scintigraphy, then an accurate assessment of renal function is considered possible with an improved diagnostic ability.

Phytochemical profiles of Roots of Buckwheat Extract

Young-Min Ham,Seon-A Yoon,Weon-Jong Yoon,Woo-Sam Yang,Bo Ram Go,Dae-Ju Oh 한국식품영양과학회 2018 한국식품영양과학회 학술대회발표집 Vol.2018 No.10

The CH2Cl2<i></i>Extract Fraction from<i> Ficus erecta</i> var. <i>sieboldii</i> (Miq.) King Suppresses Lipopolysaccharide-mediated Inflammatory Responses in Raw264.7 Cells

Ham, Young-Min,Yoon, Weon-Jong,Hwa Sohn, Eun,Won Park, Dae,Jeon, Hyelin,Jung, Yong-Hwan,Ryul Lee, Sung,Chan Kang, Se Science and Education Publishing Co., Ltd. 2018 Journal of food and nutrition research Vol.6 No.6

Anti-inflammatory Activities of the Compound Isolated from Undaria peterseniana in LPS-stimulated RAW 264.7 cells

Young-Min Ham,Sang-Mok Song,Dae-Ju Oh,Seon-A Yoon,Cang-Sik Ko,Jong-Cheol Lee,Chang-Sook Kim,Weon-Jong Yoon 한국식품영양과학회 2015 한국식품영양과학회 학술대회발표집 Vol.2015 No.8