Slide Session : OS-IFD-07 ; Infectious Disease : In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus

( Myung Jin Lee ),( Kye Hyung Kim ),( Jong Youn Yi ),( Su Jin Choi ),( Chung Jong Kim ),( Nak Hyun Kim ),( Kyoung Ho Song ),( Pyoeng Gyun Choi ),( Ji Hwan Bang ),( Wan Beom Park ),( Eu Suk Kim ),( San 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1

In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus Myung Jin LEE1, Kye-Hyung KIM1, Jongyoun YI2, SuJin CHOI1, Chung-Jong KIM1, Nak- Hyun KIM1, Kyoung-Ho SONG1, Pyoeng Gyun CHOI1, Ji-Hwan BANG1, Wan Beom PARK1, Eu Suk KIM1, Sang-Won PARK1, Hong Bin KIM1, Nam Joong KIM1, Myoung- Don OH1 Seoul National University College of Medicine, Korea1, Pusan National University School of Medicine, Korea2 Background: Severe fever with thrombocytopenia syndrome (SFTS) is an emerging infectious disease caused by a novel Bunyavirus, severe fever with thrombocytopenia syndrome virus (SFTSV). No effective antiviral therapy is proven yet, but clinical use of ribavirin (RBV) has been tried. We investigated the antiviral effect of RBV against SFTSV in vitro. Methods: To test for cytotoxicity of RBV, Vero cells were treated with different concentrations of RBV (3.90 to 500 μg/mL, two-fold dilution) and analyzed by cell viability MTS assay 48h post-infection. To determine antiviral activity of RBV against SFTSV, Vero cells were infected with SFTSV strain Gangwon/Korea/2012 at 100 TCID50 (50% tissue culture infective dose) per well in a 96-well plate, and RBV was added at the concentrations showing no or minimal cytotoxicity. Viral RNAs were extracted from the culture supernatants and quantifi ed using one-step real-time reverse transcription- PCR to amplify the partial large segment of SFTSV. Statistical analysis was done by one-way ANOVA with Tukey`s post hoc test. Results: Cytotoxicity due to RBV was not observed at RBV concentration =31.3 μg/ mL. Viral RNAs at 24h post-RBV treatment were reduced with increasing RBV concentrations (1-32 μg/mL), compared with those of mock-treated cells (P <0.01, Figure). Half maximal inhibitory concentration (IC50) of RBV was 3.69 μg/mL at 24h post-RBV treatment. Conclusions: Our study shows that RBV has antiviral effect against SFTSV in a dose-dependent manner. Further studies are required to evaluate the effi cacy of RBV in SFTS.

부산지역 수돗물과 지하수의 중금속 농도

김준연,손지언,김형수,김두희,원미숙,김인식,이혜령 동아대학교 산업의학연구소 2000 산업의학연구소 논총 Vol.- No.5

This study investigated mean airborne CO concention of 15 workplaces, suspected of CO exposure and conducted self-reported questionnaire completion and indirect COHb concentration measure using Micro II Smokerlyzer to healthy 702 adult subjects from 1999 May to 1999 September in order to find a relation of CO exposure and occupational factors, socioeconomic factors, and health related behaviors and confirm the related conditions in the screening test for CO exposure. The results of this study were summarized as follows : 1. In the CO exposed and non exposed group, COHb concentrations of the smokers were 2.55±0.96% and 2.21±0.97% and that got a statistically significant difference, There were not statistically significant differences in the age and total smoking index. Passed times after the last smoking, Working time/day, and working duration were statistically significant difference (p〈0.05). 2. In the CO exposed and non exposed group, COHb concentrations of non-smoking group were 0.94±0.35% and 0.68±0.47% and that got a statistically significant difference. There were not statistically significant differences in the age and working duration but Working time/day was a statistically significant difference(p〈0.05). 3. In the CO exposed group, r-square of multiple regression of the smokers was 38.5 % and passed time after the last smoking, working time/day, and job category were statistically significant differences (p〈0.05). And r-square in the CO non-exposed group was 38.3 % and age, passed time after the last smoking, and total smoking index were statistically significant difference. 4. In the CO exposed group, r-square of multiple repression of the non-smokers was 66.3% and job category and airborne CO concentration were statistically significant differences(p〈0.05). But r-square of non-smokers in the CO non-exposed group was 1.0% and there was not a statistically significant difference(p〈0.05). 5. In the smokers of CO exposed and non exposed groups, relation of COHb concentration and passed time after the last smoking was expressed as exponential function, Y = 2.9182e-0.0083x and r-square of this function was 37.4%. Therefore it was more than 150 minutes that passed time after the last smoking was when COHb concentrations were measured as less than 1%. In conclusion, variable, that was statistically significant to COHb concentraion in the both CO exposed and non-exposed smokers, was a passed time after the last smoking. We suggest that you have to restrict the smoking of smokers at least 150 minutes in the exposed and non exposed group before COHb concentration measure in order to exclude smoking effects

유제놀에 의한 즉시형 알레르기 반응의 억제

김상현,신태용,김형룡,이영미,이은희,신보경,김윤철,안년형,김형민 우석대학교 의약품개발연구소 1996 藥學硏究誌 Vol.1 No.-

The current study evaluates the capacity of eugenol to regulate immediate allergic reaction by control of histamine release. Administrations of eugenol (1 M/kg. i.p.) at 60 min before and 5.10 min after the compound 48/80 treatment (8 mg/kg.i.p.) were shown the mortality rates as 0.44.4. and 77.8%. respectively. A 60 min before administered group revealed a singificant inhibition of serum his-tamine release compared with those of 5 and 10 min after the compound 48/80 injection. Eugenol (6-48mM) was also showed a dose-dependent activity on the compound 48/80-induced histamine release from the highly purified population of Alcian Blue-positive peritoneal mast cells. These results indicate that in vitro treatment with exogenous eugenol inhibited the active response of mast cell populations and modulated its characteristics.

하지 분절 각도에 따른 수의 등척성 수축(MVIC)시 근전도 비교

김정자,이민형,김연정,채원식,한윤수,권선옥 한국운동역학회 2005 한국운동역학회지 Vol.15 No.1

J. J. KIM, M. H. LEE, Y. J. KIM, W. S. CHAE, Y. S. HAN, O. KWON. Comparison of the maximum EMG levels recorded in maximum effort isometric contractions at five different knee flexion angles. Korean Journal of Sport Biomechanics, Vol. 15, No. 1, pp. 197-206, 2005. The purpose of this study was to quantify the maximum EMG levels and determine if there are differences in these EMG levels with respect to different knee flexion angles. Eight university students with no known musculoskeletal disorders were recruited as the participants. The maximum voluntary isometric knee extensions and flexions were taken from each participant sat on the isokinetic exercise machine (Cybex 340) at five different knee flexion angles (10?30?50?70? 90?. After surface electrodes were attached to rectus femoris, vastus medialis, vastus laterlis, biceps femoris, and semitendinosus, maximum EMG levels at five different knee flexion angles were measured. The results showed that there was no significant difference in maximum EMG levels among five different knee flexion angles. Although there was no significant difference in EMG levels and were some variations among different knee flexion angles, the EMG signals of quadriceps in extension and biceps femoris in flexion were the greatest at 30? It seems that different joint angles or relative locations of body segments might affect the magnitude of EMG levels. Because the maximum EMG levels could change with a different knee flexion angle, an attempt should be made to more accurately measure these values. If then, %MVIC measure provides more reliable data and is most appropriate for EMG normalization.

농산물 소독과정에서 브롬화메틸에 노출된 근로자에게 발생한 신경병 2례

박태혁,김정일,손지언,김종국,김형수,정갑열,김준연 大韓産業醫學會 2000 대한직업환경의학회지 Vol.12 No.4

목적 : 농산물 소독과정에서 브롬화메틸에 노출된 근로자에게 발생한 신경병 2례 보고. 방법 : 피로감, 전신무력감, 어지럼증, 보행장애 등의 증상을 주소로 내원한 브롬화메틸을 사용하여 방역작업에 종사하는 건강한 20세와 18세의 근로자를 대상으로 혈액검사, 신경정도 및 근전도검사, 뇌자기공명영상 등의 임상검사와 작업력조사를 시행하였다. 결과 : 1례에서 뇌자기공명영상에서 연수와 소뇌의 양측 측충부의 고신호강도가 관찰되었고 11일 후의 추적조사에서 동일 부위의 고신호강토가 감소된 소견이 관찰되었으며 뇌병증으로 최종 진단되었다. 다른 1례는 신경전도검사에서 다발성 신경병 소견과 뇌유발전위검사에서 우측 하부 뇌간부위 병소가 관찰되었고 뇌병증 및 말초신경 병으로 최종 진단되었다. 결론 : 근로자들의 증상은 소독과정에서 브롬화메틸 노출로 인한 신경 병으로 판단되며 훈증소독과방역작업에 종사하는 근로자들의 실태조사와 안전에 대한 대책이 시급하다고 하겠다. Objectives : To report two cases of neuropathy due to methyl bromide intoxication. Methods : Workers, engaged in the fumigating process, complained fatigue, general weakness, ataxia, and hypersomnia. We evaluated them with blood tests, neurophysiologic studies and MRI and investigated their occupational history. Results : Increased signal intensities were found in the medulla oblongata and paraver mian of cerebellum in MRI and after 11 days, high signal intensities were reduced in the following MRI. In the other case, polyneuropathy and rlght lower brainstem lesion were observed In the NCV and BAEP studies. Conclusions : We confirmed that worker's symptoms were related to methyl bromide exposure in the fumigation. It is necessary that we should evaluate present condition of fumigating process and prepare appropriate methods to protect workers engaged in the fumigation.

연령에 따른 한국인의 음향지표 변화와 특성

김형태,조승호,윤성문,선동일,김민식 대한이비인후과학회 2000 대한이비인후과학회지 두경부외과학 Vol.43 No.1

전방경추부 응합술시 단피질 나사못과 양피질 나사못의 비교연구

박형천,윤승환,박현선,김은영 대한신경외과학회 1998 Journal of Korean neurosurgical society Vol.27 No.5

合成洗劑가 빨간집모기의 生態, 生理學的 特性에 미치는 影響

金正和,李炯來,朴垠澈,李容揆,崔觀善 충북대학교 농업과학기술연구소 1995 農業科學硏究 Vol.12 No.2

It is considered a general experience that migration from rural to urban areas will go on in any developing countries pursuing economic development through industralization. In Korea, migration is considered one of the serious socio- economic problems because it has been made excessively fast than any other countries' experiences. The socio- economic problems originates in migration can be classified in two ways ; the one is the overpopulated problems of urban areas and emptied villages problems of rural areas, and the other is the shartage agricultural labor farce problems symbolized by the increasing idle cultivated land. To relieve migration effectively, the developing policies should be converted from pursuing fast industralization and urbanization to balance growth between areas and industries. Moreover problem solving political measure should be converted from partial and corresponding approaches to total approaches containing economic, social and institutional side-view.

천마의 항 불안 효과

김여환,최형철,손의동,이광윤,김원준,박형배,하정희 대한생물치료정신의학회 1996 생물치료정신의학 Vol.2 No.2

천마(Gastrodia elata Blume)의 근경을 말린 것은 한방에서 오래전부터 여러 형태의 간질발작 치료 목적으로 이용되어지고 있었으나 어떤 활성물질에 의해 또 어떤 작용 기전을 통해 약리작용을 나타내는지를 충분하게 설명하지 못하고 있다. 최근 항경련 작용기전 연구 결과 천마추출물의 GABA성 신경전달계에 대한 조절작용과 관련이 있을 것이라는 연구 결과가 보고되었으며, 소량으로도 뇌억제 및 진정작용이 있다는 문헌을 참고하여 볼 때 천마의 항불안 작용 가능성이 시사되었다. 이에 본 연구를 통하여 천마를 사용하여 실험동물에서 항불안 작용을 검색하고 그 작용기전의 일부를 밝히고자 항불안작용 기전에 중요한 benzodiazepine 수용체와의 상호작용을 관찰하였다. 실험 결과, 천마 추출물의 경구 장기투여는 생쥐의 elevated plus maze 검사에서 개방 통로에로의 진입 및 개방통로에서의 체류시간을 증가시킴으로써 항불안 효과를 나타내었다. 천마의 에탄올 추출물은 benzodiazepine수용체 길항제인 [³H] Ro15-1788결합을 억제하였으며, 천마 추출물은 [³H] Ro15-1788의 수용체 결합에 대한 최대 결합력(Bmax)은 변화시키지 않고, 친화력(affinity)을 감소시킴으로써 상경적인 결합 양상을 나타내었다. 또 천마의 에탄올 추출물은 benzodiazepine 수용체의 효현제인 flunitrazepam의 수용체 결합반응을 억제시켰는데, 이러한 억제는 GABA 존재하에서 항진되는 것을 관찰하였다. 이상의 결과로 미루어보아 천마의 에탄올추출물 내에는 항불안작용을 나타내는 성분이 함유되어 있을 가능성을 예상케한다. Gastrodia elata Blume is a medicinal plant which has been used as anticonvulsant in oriental medicine, and has been used as sedatives. A survey of the relevant literature has indicated that the putative anxiolytic activity of G.elata Bl. has not been scientifically investigated. Therefore, the present study was designed to assess anxiolytic property and interaction with benzodiazepine receptor of G. elata BI. The putative anxiolytic activity of ethanol extract of G. elata BI. was performed in mice using an elevated plus maze paradigm. Chronic oral administration of G. elata BI. showed anxiolytic action in mice. It was suggested that regulation of GABAergic neurotarnsmission may be important in the action of G. elata BI. The interaction of G. elata BI. with benzodiazepine receptor was investigated using rat cortices. Ethanol extract of G. elata inhibited the binding of [³H] Ro15-1788, a selective benzodiazepine receptor antagonist to benzodiazepine receptor. The inhibition of [³H] Ro15-1788 binding by G. elata BI. appeared to be competitive. Further, GABA significantly enhanced the potency of this extract in inhibiting [³H] flunitrazepam, a selective benzodiazepine receptor agonist, binding to benzodiazepine receptor. From these findings, it can be concluded that substance or substances with neurochemical properties characteristic of a benzodiazepine receptor agonist may contribute to the anxiolytic property of G. elata BI.

간접골성 고정원을 이용한 상악 구치부 원심이동 장치 종류에 따른 치아 이동 양상 평가

김수진,전윤식,정상혁,박선형 대한치과교정학회 2008 대한치과교정학회지 Vol.38 No.6

본 연구의 목적은 pendulum 장치, 미니임플란트를 동반한 pendulum장치(펜듈럼), 오픈코일 스프링 및 미니임플란트를 동반한 오픈코일 스프링 이용 시 각각의 치아이동 양상을 3차원적으로 분석하는 데 있었다. 상악 좌측 치조골 및 치아 모형을 제작하고, Calorific machine을 이용하여 모형상에서 대구치를 3 mm 원심이동시켰다. 실험은 5회씩반복 실시하였다. 모델을 전산화 단층 촬영한 후 V-Works를 이용하여 3차원 모델을 제작하였다. Rapidform상에서 3차원적으로 이동방향과 이동량을 계측하였고, 각각의 장치에 관한 통계적 유의성을 검정하였다. 교정용 미니임플란트를 간접 골성 고정원으로 이용하여 오픈코일 스프링으로 구치부를 원심으로 이동시켰을 때가 치체이동에 가장 가까운 이동양상을 보였고, 고정원 소실도 적게 나타났다 (p < 0.05). 오픈코일 스프링, 펜듈럼 장치 모두 미니임플란트를 부가적으로 이용했을 때 고정원 소실량이 적었다 (p < 0.05). 미니임플란트를 이용하지 않은 경우에는 두 장치의 고정원 소실량이 비슷하였다. 미니임플란트의 이용 여부와 관계없이 펜듈럼 장치로 상악 구치 원심 이동 시 오픈코일 스프링에 비해 제1대구치가 조절성 경사이동 양상으로 이동되었고 (p < 0.05), 제2대구치는 비조절성 경사이동 양상을 보였으며, 치관의 협측경사이동이 일어났다 (p < 0.05). 이와 같은 결과를 근거로 간접 골성 고정원을 이용한 오픈코일 스프링이 상악 구치의 원심 치체이동에 가장 효과적인 장치였으며, 펜듈럼 장치를 이용한 구치부 원심이동 시에는 추가적인 조절이 필요하다고 할 수 있다. Objective: The purpose of this study was to compare the three dimensional changes of tooth movement using four different types of maxillary molar distalization appliances; pendulum appliance (PD), mini-implant supported pendulum appliance (MPD), stainless steel open coil spring (SP) and mini-implant supported stainless steel open coil spring (MSP). Methods: These experiments were performed using the Calorific machine which can simulate dynamic tooth movement. Computed tomography (CT) images of the experimental model were taken before and after tooth movement in 1 mm thicknesses and reconstructed into a three dimensional model using V-works 4.0. These reconstructed images were superimposed using Rapidform 2004 and the direction and amount of tooth movement were measured. Results: The mean reciprocal anchor loss ratio at the first premolar was 17 - 19% for the PD and SP groups. The appliances using mini-implants (MPD or MSP) resulted in less anchorage loss (7 - 8%). On application of a pendulum appliance or MPD, distalization was obtained by tipping rather than by bodily movement. Furthermore, the maxillary second molar tipped distally and bucally. But on application of MSP, distalization was achieved almost by bodily movement. Conclusions: Regarding tooth movement patterns during molar distalization, stainless steel open coil spring with indirect skeletal anchorage was relatively superior to other methods.