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Hur, Yeoun,Tae, Sookil,Koh, Yun-Joo,Hong, Sung-Hyun,Yoon, Young Ho,Jang, Haejong,Kim, Sooji,Kim, Kyeong Ho,Kang, Seung Woo,Lee, Youngshin,Han, Sang Beom Korean Society for Mass Spectrometry 2014 Mass spectrometry letters Vol.5 No.2
A specific and sensitive liquid chromatography-electrospray ionization tandem mass spectrometry method (LC-ESI-MS/MS) was developed and validated for the simultaneous quantification of porphyrins (coproporphyrin, pentacarboxylporphyrin, hexacarboxylporphyrin, heptacarboxylporphyrin, and uroporphyrin) in human plasma and urine. Acidified plasma samples and urine samples were prepared by using liquid-liquid extraction using ethyl acetate and protein precipitation with acetonitrile, respectively. The separation was achieved onto a Synergi Fusion RP column ($150mm{\times}2.0mm$, $4{\mu}m$) with a gradient elution of mobile phase A (0.1% formic acid in 2 mmol/L ammonium acetate, v/v) and mobile phase B (20% methanol in acetonitrile, v/v) at a flow rate of $450{\mu}L$/min. Porphyrins and the internal standard (IS), coproporphyrin I-$^{15}N_4$, were detected by a tandem mass spectrometer equipped with an electrospray ion source operating in positive ion mode. Multiple reaction monitoring (MRM) transitions of the protonated precursor ions and the related product ions were optimized to increase selectivity and sensitivity. The proposed method was validated by assessing selectivity, linearity, limit of quantification (LOQ), precision, accuracy, recovery, and stability. The calibration curves were obtained in the range of 0.1-100 nmol/L and the LOQs were estimated as 0.1 nmol/L for all porphyrins. Results obtained from the validation study of porphyrins showed good accuracy, precision, recovery, and stability. Finally, the proposed method was successfully applied to clinical studies on the autism spectrum disorder (ASD) diagnosis of 203 Korean children.
( Yeoun Hur ),( Sookil Tae ),( Yun Joo Koh ),( Sung Hyun Hong ),( Young Ho Yoon ),( Haejong Jang ),( Sooji Kim ),( Kyeong Ho Kim ),( Seung Woo Kang ),( Youngshin Lee ),( Sang Beom Han ) 한국질량분석학회 2014 Mass spectrometry letters Vol.5 No.2
A specific and sensitive liquid chromatography-electrospray ionization tandem mass spectrometry method (LC-ESIMS/ MS) was developed and validated for the simultaneous quantification of porphyrins (coproporphyrin, pentacarboxylporphyrin, hexacarboxylporphyrin, heptacarboxylporphyrin, and uroporphyrin) in human plasma and urine. Acidified plasma samples and urine samples were prepared by using liquid-liquid extraction using ethyl acetate and protein precipitation with acetonitrile, respectively. The separation was achieved onto a Synergi Fusion RP column (150 mm × 2.0 mm, 4 μm) with a gradient elution of mobile phase A (0.1% formic acid in 2 mmol/L ammonium acetate, v/v) and mobile phase B (20% methanol in acetonitrile, v/ v) at a flow rate of 450 μL/min. Porphyrins and the internal standard (IS), coproporphyrin I-15N4, were detected by a tandem mass spectrometer equipped with an electrospray ion source operating in positive ion mode. Multiple reaction monitoring (MRM) transitions of the protonated precursor ions and the related product ions were optimized to increase selectivity and sensitivity. The proposed method was validated by assessing selectivity, linearity, limit of quantification (LOQ), precision, accuracy, recovery, and stability. The calibration curves were obtained in the range of 0.1-100 nmol/L and the LOQs were estimated as 0.1 nmol/L for all porphyrins. Results obtained from the validation study of porphyrins showed good accuracy, precision, recovery, and stability. Finally, the proposed method was successfully applied to clinical studies on the autism spectrum disorder (ASD) diagnosis of 203 Korean children.
이지연(Ji Yeoun Lee),정문모(Moon Mo Jung),허문회(Moon Hye Hur),안문규(Moon Kyu Ahn) 대한약학회 1998 약학회지 Vol.42 No.3
A method for the determination of basic drugs based on their reaction with picric acid to form an ion-association complex extractable into several plasticizers was developed. Basic drugs-picric acid complexes in acid medium could be extracted quantitatively into several plasticizers except phosphates. For example, the chlorpromazine-picric acid complex showed maximum absorbance at near 410nm and was applied to extraction spectrophotometric determination of chlorpromazine. The calibration curves are linear (r>0.998) within a range from 10-6 to 5 X 10-4M and the precision of the method was acceptable because RSD was less than 2.6% (n=7). The factors affecting the extraction system was discussed.
2,2' -디히드록시설포 아조메친 유도체에 의한 알미늄 및 갈륨의 형광 정량
이종원,이지연,허문회,안문규 慶星大學校 1998 論文集 Vol.19 No.2
Four azomethine dyes were synthesized in order to get excellent fluorescent reagent having hydroxyl and sulfo substituents. They are 2-hydroxy-3-sulfo-5chloroaniline-N-salicylidene(Ⅰ), 2-hydroxy-3-sulfo-5chloroaniline-N-2, 4-dihydroxybenzylidene (Ⅱ), 2-hydroxy-5-sulfoaniline-N-salicylidene(Ⅲ) and 2-hydroxy-5-sulfoaniline-N-2, 4-dihydroxybenzylidene(Ⅳ). These compounds react with aluminum and gallium in an aqueous dimethyl-formamide solution to form fluorescent 1:1 (metal-azomethine) complexes. Several conditions, such as the pH of the solution, the reagent concentration, the time of standing and fluoresence characteristics cincellar media were investigated. the maxium fluorescence intensity of the aluminum complex was found at pH 6 (heated for 10 min, at 50℃), and the gallium complex was at pH 4. The fluorescence was stable at least for 2 hours by using 2-hydroxy-5sulfoanilin-2-, 4-dihydroxybenzylidene in Triton X-100 media, 0.1∼4 ㎍ of aluminum and 0.5∼30 ㎍ of gallium in 25 mL solution could be determined. Ni(Ⅱ), Cu(Ⅱ), Fe(Ⅱ), Mg(Ⅱ), and Zn(Ⅱ) ions interfered with the determination. On the other hand, the effect of surfactants on the fluorescence characteristics in Triton X series media show a remarkable enhancement of fluorescence intensity as compared with that of azomethine complexes in aqueous media.
Dibucaine-금속 요오드 착물을 이온교환체로서 이용한 Dibucaine의 정량
최현영,이지연,허문회,안문규 慶星大學校 1998 論文集 Vol.19 No.1
Dibucaine-selective poly(vinyl chloride) menbrance electrodes were designed based on ion-association complex between dibucaine and metal iodide complex such as H??, Bil?? and CdI??. Stable potentiometric response was obtained with Meyer reagent at pH 3.0-5.5, with Dragendorff reagent at pH 3.0-5.0 and with Marume reagent at pH 3.0-5.5. The best plasticizer was 49 w/w% acetyl-tri-n-butly critrate for Meyer reagent, 65.3 w.w% for Marume reagent. The electrodes exhibited a linear response based on Meyer, Dragendorff and Marume complex were the concentration range of 2×10?4×10?M ,2×10×4×10?M, 2×10?1×10?M,respectively. The potentiometric response slope of optimized membrane electrodes based on Meyer, Dragendorff and Marume complex for dibucaine were 56.12,57.81 and 56.45?? with relative standard deviation of 2.75, 2.29 and 1.96%, respectively. This ISE methods are found to be sensitive, rapid, fairly accurate and are able to be empolyed successfully for the determination of dibucaine preparations.
A computational approach for identifying pathogenicity islands in prokaryotic genomes
Yoon, Sung Ho,Hur, Cheol-Goo,Kang, Ho-Young,Kim, Yeoun Hee,Oh, Tae Kwang,Kim, Jihyun F BioMed Central 2005 BMC bioinformatics Vol.6 No.-
<P><B>Background</B></P><P>Pathogenicity islands (PAIs), distinct genomic segments of pathogens encoding virulence factors, represent a subgroup of genomic islands (GIs) that have been acquired by horizontal gene transfer event. Up to now, computational approaches for identifying PAIs have been focused on the detection of genomic regions which only differ from the rest of the genome in their base composition and codon usage. These approaches often lead to the identification of genomic islands, rather than PAIs.</P><P><B>Results</B></P><P>We present a computational method for detecting potential PAIs in complete prokaryotic genomes by combining sequence similarities and abnormalities in genomic composition. We first collected 207 GenBank accessions containing either part or all of the reported PAI loci. In sequenced genomes, strips of PAI-homologs were defined based on the proximity of the homologs of genes in the same PAI accession. An algorithm reminiscent of sequence-assembly procedure was then devised to merge overlapping or adjacent genomic strips into a large genomic region. Among the defined genomic regions, PAI-like regions were identified by the presence of homolog(s) of virulence genes. Also, GIs were postulated by calculating G+C content anomalies and codon usage bias. Of 148 prokaryotic genomes examined, 23 pathogenic and 6 non-pathogenic bacteria contained 77 candidate PAIs that partly or entirely overlap GIs.</P><P><B>Conclusion</B></P><P>Supporting the validity of our method, included in the list of candidate PAIs were thirty four PAIs previously identified from genome sequencing papers. Furthermore, in some instances, our method was able to detect entire PAIs for those only partial sequences are available. Our method was proven to be an efficient method for demarcating the potential PAIs in our study. Also, the function(s) and origin(s) of a candidate PAI can be inferred by investigating the PAI queries comprising it. Identification and analysis of potential PAIs in prokaryotic genomes will broaden our knowledge on the structure and properties of PAIs and the evolution of bacterial pathogenesis.</P>