모과내 기능성 유용성분 용매추출공정의 최적화

전주영 ( Ju Yeong Jeon ),조인희 ( In Hee Jo ),경현규 ( Hyun Kyu Kyung ),김현아 ( Hyun A Kim ),이창민 ( Chang Min Lee ),최용희 ( Yong Hee Choi ) 한국산업식품공학회 2010 산업 식품공학 Vol.14 No.2

본 연구에서는 모과내의 여러 가지 기능성 유용성분을 효과적으로 추출하기 위해서, 모과나무의 익은 열매로 만든 약재인 모과를 사용 하였다. 모과의 기능성 유용성분용매 추출 공정의 최적 조건을 확립하고자 하였다. 모과를 에탄올에 추출하여 반응표면 분석법으로 모니터링하여 최적 용매 조건을 설정하였다. 중심합성계획법에 따라 시료에 대한 용매비(X1)와 추출온도(X2), 추출시간(X3)을 요인변수로 하고 추출수율(Y1), 총페놀 함량(Y2), 전자공여능(Y3), 갈색도(Y4), 환원당(Y5)을 종속변수로 하여 시행하였다. 실험 결과 추출수율은 추출 온도와 추출 시간에 유의하게 영향을 받음을 알 수 있었다. 안장점에서 추출조건은 시료에 대한 용매비는 26.38mL/g, 추출온도는 72.82oC, 추출시간은 74.86 min에서 최대값을 나타내었다. 총페놀 함량은 용매비와 시간에 영향을 거의 받지 않았고 추출시간에는 영향을 받았으며, 최대값은 20.70mg/mL 로 나타났다. 이때의 추출조건은 시료에 대한 용매비는 22.61mL/g, 추출온도는 84.49oC, 추출시간은 77.25 min으로 나타났다. 전자공여능은 추출온도에 따라 유의하게 영향을 받은 것으로 나타났다. 안장점에서의 추출조건인 시료에 대한 용매비 10.65mL/g, 추출온도 67.78oC, 추출시간 96.75 min에서 추출수율은 94.12%로 예측되었다. 갈색도에 대한 추출조건은 시료에 대한 용매비 23.77mL/g, 추출온도 87.27oC, 추출시간 96.68 min 일 때 안장점이 나타났다. 환원당은 시료에 대한 용매비 26.83mL/g, 추출온도 82.167oC, 추출시간 81.94 min에서 10.55mg/mL로 최대값을 나타내었고 추출시간에 영향을 받았다. In this study, various active functional components in Chinese Quince were extracted by solvent extraction method. A central composit design for optimization was applied to investigate the effects of independent variables such as solvent to sample ratio (X1), extraction temperature (X2), and extraction time (X3) on the soluble solid contents (Y1), total phenols (Y2), electron donating ability (Y3), browning color (Y4) and reducing sugar contents (Y5). It was found that extraction temperature and extraction time were the main effective factors in this extraction process. The maximum soluble solid contents of 35.77% was obtained at 26.38mL/g (X1), 72.82oC (X2) and 74.86 min (X3) in saddle point. Total phenols were rarely affected by solvent ratio and extraction time, but it was affected by extraction temperature. The maximum total phenols of 20.70% was obtained at 22.61mL/g (X1), 84.49oC (X2), 77.25 min (X3) in saddle point. The electron donating ability was affected by extraction time. The maximum electron donating ability of 94.12% was obtained at 10.65mL/g (X1), 67.78oC (X2), 96.75 min (X3) in saddle point. The maximum browning color of 0.32% was obtained at 23.77mL/g (X1), 87.27oC (X2), 96.68 min (X3) in saddle point. The maximum value of reducing sugar content of 10.55% was obtained at 26.83mL/g (X1), 82.167oC (X2), 81.94 min (X3). Reducing sugar content was affected by extraction time.

파라핀 오일을 이용한 W/O 에멀젼법하에 구형 γ-알루미나 분체 제조시 유화제 종류 및 조성변화의 영향

이융,이상우,이창일,함영민 한국공업화학회 2002 응용화학 Vol.6 No.2

In this work, the average particle size, the degree of monodispersity of particle size and the morphology of v-A1_2O_3 powder were investigated by changing the type and composition of emulsifiers under 0/W emulsion method. Water phase(W) was aq. v-A1_2O_3 sol of pH=3 and oil phase(O) was paraffin oil containing diverse main surfactants and[or] cosurfactants, which were sp8O, tw80, n-butanol, n-octanol and n-dodecanol. The mixing rate & mixing time and the O:W volumetric ratio to form emulsion were 2,000rpm & lhr and 8:1, respectively. As results, the morphology of v-A1_2O_3 powder prepared under all conditions was spherical form and the min. average particle size, 0.56㎛, was obtained when mixed surfactants of 2vol.%[sp80 & tw8O] of HLBm=5 were used. and the max. monodispersity of particle size, 1.06, was achieved when 0.4vol.%n-butanol, as a cosurfactant, was used with 2vol.%[sp80 & tw8O] of HLBm=5.

Visualization and Quantification of Radiochemical Purity by Cerenkov Luminescence Imaging

Ha, Yeong Su,Lee, Woonghee,Jung, Jung-Min,Soni, Nisarg,Pandya, Darpan N.,An, Gwang Il,Sarkar, Swarbhanu,Lee, Won Kee,Yoo, Jeongsoo American Chemical Society 2018 ANALYTICAL CHEMISTRY - Vol.90 No.15

<P>Determination of radiochemical purity is essential for characterization of all radioactive compounds, including clinical radiopharmaceuticals. Radio-thin layer chromatography (radio-TLC) has been used as the gold standard for measurement of radiochemical purity; however, this method has several limitations in terms of sensitivity, spatial resolution, two-dimensional scanning, and quantification accuracy. Here, we report a new analytical technique for determination of radiochemical purity based on Cerenkov luminescence imaging (CLI), whereby entire TLC plates are visualized by detection of Cerenkov radiation. Sixteen routinely used TLC plates were tested in combination with three different radioisotopes (<SUP>131</SUP>I, <SUP>124</SUP>I, and <SUP>32</SUP>P). All TLC plates doped with a fluorescent indicator showed excellent detection sensitivity with scanning times of less than 1 min. The new CLI method was superior to the traditional radio-TLC scanning method in terms of sensitivity, scanning time, spatial resolution, and two-dimensional scanning. The CLI method also showed better quantification features across a wider range of radioactivity values compared with radio-TLC and classical zonal analysis, especially for β<SUP>-</SUP>-emitters such as <SUP>131</SUP>I and <SUP>32</SUP>P.</P> [FIG OMISSION]</BR>

혈액 투석 환자에서 중심정맥 협착에 대한 스텐트 삽입술 : Wallstent Placement

임대승,노상필,이유선,정승현,김보영,이정우,강정아,김정희,이민수,정준용,최시완,정진옥,성인환,이강욱,신영태 충남대학교 의과대학 의학연구소 2002 충남의대잡지 Vol.29 No.1

Stenosis of central vein is a common complication arising after percutaneous subclavian vein catheter insertion performed for temporary vascular access in chronic renal failure patients undergoing hemodialysis. There are several treatment methods for the condition like percutaneous angioplasty(PTA), stent insertion, and surgery, but recent trend is toward PTA and stents. Among the patients diagnosed with chronic renal failure from March 1993 to May 2002 and undergoing hemodialysis through AV fistula, the 14 Patients in whom central vein stenosis arose were selected for the study. A total of 28 percutaneous interventions(5 PTA and 23 stent placement) were performed, and restenosis rate and the time taken till the restenosis in de novo lesions and instant lesions were compared. All 28 cases were operated successfully. The 14 cases that received both anigioplasty and stent placement initially. (de novo lesion : 14 cases), Among the 10 cases with de novo lesion that followed up more than 1 year, 3 cases are currently undergoing hemodialysis without restenosis, and the remaining 7 cases have recurred stenosis with the mean time to restenosis of 10.9 months. In the 7 cases in whom stenosis recurred, 11 interventions were done(instent lesion: 11 cases). 4 of these were using only ballon angioplasty with 100% restenosis rate and the mean time of 3 months until restenosis. The remaining 7 cases were using both balloon angioplasty and stent placement, also with 100% restenosis rate but with the mean time of 12 months until restenosis, which was later than the group receiving only balloon angioplasty. In treating the patients with central vein stenosis, stent placement seems to be more advantageous over PTA in terms of restenosis rate and the mean duration of patency. In the case of instent lesion, inserting the stent for the second time after stenosis recurred lengthened the duration of patency compared to performing balloon angioplasty alone.

Improved Properties of Nanocrystalline Cellulose Isolated from Kraft Pulp by Electron Beam Irradiation

Kim, Sun-Young,Lee, Byoung-Min,Lee, Yeong-Ju,Kim, Du-Yeong,Jeun, Joon-Pyo,Kang, Phil-Hyun American Scientific Publishers 2016 Journal of nanoscience and nanotechnology Vol.16 No.10

<P>In this study, we investigated an improvement to the properties of nanocrystalline cellulose (NCC) from kraft pulp using electron beam irradiation (EBI). The EBI of the kraft pulp was performed at various doses (25, 50, and 75 kGy) and then hydrolyzed with 55% sulfuric acid at 50 degrees C (preheated) for 20 min. The hydrolysate of kraft pulp was characterized using X-ray diffraction (XRD), particle size distribution, thermogravimetric analysis (TGA), and transmission electron microscopy (TEM). The NCC of pristine kraft pulp was obtained with a broad size distribution (0-700 nm), an average particle size of 220 nm, and a 75.5% crystallinity index after 20 min of acid-hydrolysisat 50 degrees C. However, isolated NCC from 75 kGy irradiated kraft pulp was obtained with a narrow size distribution (0-530 nm), an average particle size of 160 nm, and 83.2% crystallinity after 20 min of acidhydrolysisat 50 degrees C. These results indicate that the size and crystallinity of NCC were easily controlled by adjusting the EBI dose.</P>

Radicicol Inhibits iNOS Expression in Cytokine-Stimulated Pancreatic Beta Cells

Cha Kyung Youn,Seon Joo Park,Mei Hong Li,Min Young Lee,Kun Yeong Lee,Man Jin Cha,Ok Hyeun Kim,Ho Jin You,In Youp Chang,Sang Pil Yoon,Young Jin Jeon 대한생리학회-대한약리학회 2013 The Korean Journal of Physiology & Pharmacology Vol.17 No.4

Here, we show that radicicol, a fungal antibiotic, resulted in marked inhibition of inducible nitric oxide synthase (iNOS) transcription by the pancreatic beta cell line MIN6N8a in response to cytokine mixture (CM: TNF-Ձ, IFN-Ճ, and IL-1Ղ). Treatment of MIN6N8a cells with radicicol inhibited CM-stimulated activation of NF-ՊB/Rel, which plays a critical role in iNOS transcription, in a dose-related manner. Nitrite production in the presence of PD98059, a specific inhibitor of the extracellular signal-regulated protein kinase-1 and 2 (ERK1/2) pathway, was dramatically diminished, suggesting that the ERK1/2 pathway is involved in CM-induced iNOS expression. In contrast, SB203580, a specific inhibitor of p38, had no effect on nitrite generation. Collectively, this series of experiments indicates that radicicol inhibits iNOS gene expression by blocking ERK1/2 signaling. Due to the critical role that NO release plays in mediating destruction of pancreatic beta cells, the inhibitory effects of radicicol on iNOS expression suggest that radicicol may represent a useful anti-diabetic activity.

Radicicol Inhibits iNOS Expression in Cytokine-Stimulated Pancreatic Beta Cells

Youn, Cha Kyung,Park, Seon Joo,Li, Mei Hong,Lee, Min Young,Lee, Kun Yeong,Cha, Man Jin,Kim, Ok Hyeun,You, Ho Jin,Chang, In Youp,Yoon, Sang Pil,Jeon, Young Jin The Korean Society of Pharmacology 2013 The Korean Journal of Physiology & Pharmacology Vol.17 No.4

Here, we show that radicicol, a fungal antibiotic, resulted in marked inhibition of inducible nitric oxide synthase (iNOS) transcription by the pancreatic beta cell line MIN6N8a in response to cytokine mixture (CM: TNF-${\alpha}$, IFN-${\gamma}$, and IL-$1{\beta}$). Treatment of MIN6N8a cells with radicicol inhibited CM-stimulated activation of NF-${\kappa}B$/Rel, which plays a critical role in iNOS transcription, in a dose-related manner. Nitrite production in the presence of PD98059, a specific inhibitor of the extracellular signal-regulated protein kinase-1 and 2 (ERK1/2) pathway, was dramatically diminished, suggesting that the ERK1/2 pathway is involved in CM-induced iNOS expression. In contrast, SB203580, a specific inhibitor of p38, had no effect on nitrite generation. Collectively, this series of experiments indicates that radicicol inhibits iNOS gene expression by blocking ERK1/2 signaling. Due to the critical role that NO release plays in mediating destruction of pancreatic beta cells, the inhibitory effects of radicicol on iNOS expression suggest that radicicol may represent a useful anti-diabetic activity.

청소년유해정보의 규제구조에 관한 행정법적 고찰

李敏榮 法務部 商事法務課 2006 선진상사법률연구 Vol.- No.36

The Seoul Administrative Court and Seoul High Court of Justice have addressed different opinions of the action for nullifying Harmful Information to luverdles decision of lCEC(Information Communication Ethics Committee). But Harmful Information to Juveniles is coming to be exposed, because the end judgement of Supreme Court does not come out. Apparently it is important that a corresponding medium scandal corpse charstteristic is yes or no, so that solution of substantial dispute should lean to a conclusive judgement. The Regulation on Harmful Information to luverdles as state affair is axomplished by ICECs dedsion through application of deliberative prescription as Directive which takes effect by notice of NYQNational Youth Commission). Consequently, the opinion that it has nature of administrative disposal is rational for relief of rights and interests according to the aim of Administrative litigation Act, on the ground that ICECs dedsion estimates harmfulness substantially at its own discretion. 정보통신은리위원회의 청소년유해정보결정에 대한 취소소송에서 서울행정법원과 서울고등법원은 다른 논리를 취하고 있다. 하지만 조속히 확정 판결이 나오지 않는 상황에서 대법원의 종국적 판단이 기다려지는 가운데 여전히 청소년유해정보가 노출되어지고 있다. 원심과 항소심에서 위 결정의 처분성으로 논란이 되고 있지만, 결국 중요한 것은 해당 매채물의 유해성 여부인 것이고 이를 본안심리에서 판단하지 아니하고 형식적인 요건에만 치우친 나머지 이론적 오류에 근거한 논리로써 실질적인 분쟁의 해결에는 적극적이지 못한 사법부의 태도는 바람직하다고 볼 수 없는 양태를 보이고 있다. 청소년유해정보에 대한 규제라는 국가사무는 위와 같은 결정이 핵심이 되는 것이지나 이는 자기완결적 행정 작용이 아니라 국가청소년위원회의 고시처분으로써 효력을 발생하는 것이며, 따라서 유해성 여부에 대한 판단은 유해정보의 결정에서 이루어진다는 점에 비추어 그 전체적 구조를 파악할 수 있다. 이에 따르면 청소년유해매체물 가운데 전기통신회선을 통해 제공되는 것은 청소년유해정보로서 이에 대한 실질적 판단은 보조기관인 사무처의 보조작용에 따라 구체적 법집행을 수행하는 정보통신부 소속 합의제행정기관인 정보통신윤리위원회의 심의로 결정되고 이것이 당사자가 다투려는 소송물이라 할 것이며, 행정기관인 정보통신윤리위원회는 재량권의 행사로 위 결정을 행하며 이에 대한 판단기준은 정보통신윤리심의규정 제5조 내지 제8조에 규정된 사항으로 그 자체는 대외적 구속력이 인정되지 않는 내부적 사무처리기준에 준하지만 이를 통해 발현되는 처분으로 인하여 행정소송이 제기되면 이를 매개로 사법부가 당해처분의 위법성 여부를 심사하게 되므로 간접적 법규성을 보유하는 이른바 재량준칙에 해당한다는 것이다.

國際競爭力 向上을 위한 技術開發 促進戰略에 관한 硏究

李丙旼,許永道,高炳云 울산대학교 1990 연구논문집 Vol.21 No.2

本硏究에서는 技術이 一國의 經濟成長 및 國際競爭力의 向上에 있어서 차지하고 있는 役割의 重要性을 再認識하고 우리나라 企業들의 技術開發을 促進하기 위한 戰略을 探索해 보고자 하였다. 이러한 戰略 探索을 위해 우리나라의 技術開發過程 및 現況을 分析하여 問題點을 導出하였고 技術開發潛在力의 國際比較를 통해 우리의 脆弱點이 무엇인가를 糾明하였다. 마지막으로 이러한 問題點과 脆弱點을 補完하기 위해서 政府, 企業, 學界는 어떤 정책과 전략을 세워야 할 것인가에 대해 제시해 보았다. The focus of this study is on a search of the strategy for accelerating technology development by Korean firms and industries through re-examining the importance of the role of technology in promoting the international competitiveness and economic growth of a country. In searching of this strategy, we extracted various problems confronting Korean firms and industries, by examining the history of technology development and by analyzing present status of the activity. And we clarified the relative weaknesses of Korean firms and industries in technology development, by comparing internationally the potentiality of technology development. Finally, we suggested the possible strategies to solve above problems and to complement above weaknesses, which may be pursued by the government, business managers and academic members.

경주 남리사지에 사용된 석재의 암석학적 특성과 원산지 해석

이찬희,정연삼,정민호,이명성,김영택 공주대학교 문화재보존과학연구소 2004 문화재과학기술 Vol.3 No.1

이 연구는 경주 남리사지에 이용된 석재를 대상으로 암석의 성인적 분류, 광물 및 지구화학적 특성을 검토하여 석재의 기원지 추정과 고고지질학적 의미를 해석한 것이다. 남리사지에서 출토된 암석은 5종의 화강암질암 (알카리 화강암, 흑운모 화강암, 미문상 화강암, 등립질 화강암, 홍장석 화강암), 세립질 퇴적암 (셰일, 미사암), 호온펠스, 옹회암질암, 염기성 맥암 등이 있다. 이 중에서 서탑에 이용된 암석은 유백색의 중립질 알카리 화강암과 담홍색의 흑운모 화강암이 주류를 이룬다. 각 석재들의 대자율 값은 흑운모 화강암이 4.94~7.59 (평균 6.47)로서 제일 높았으며 미문상 화강암이 0.86~1.54 (평균 1.13)로 제일 낮았다. 알카리 화강암은 3.51, 홍장석 화강암이 5.88, 등립질 화강암이 4.89로서 분화경로와 산출상태에 따라 조금씩 다른 값을 보였다. 이 화강암류는 화학조성으로 볼 때, 거의 동일한 마그마 기원의 칼크-알카리 계열 화강암 영역에 해당된다. 이 석재의 화학적 풍화지수와 풍화 잠재지수는 각각 49.87~61.64와 4.12~8.46 범위로서, 현재 암석이 화학적 풍화작용이 왕성하게 발생하는 정도는 아니나 풍화잠재력은 매우 높은 것으로 나타났다. 이 화강암질암의 암석학적 및 지구화학적 특징으로 볼 때, 이들의 성인적 유형은 남산 일대에 분포하는 화강암과 동일하다. 따라서 남리사지의 석조문화재 건축에 사용된 석재는 현지에서 재료를 구하고 현장에서 제작한 자급자족형 석재와 수계의 상류지역에서 도입된 외래형 석재가 공준하고 있는 것으로 해석된다. 자급형 석재가 현지의 원료를 이용한 것은 확실하나 어느 지역에서 채석하여 어디서 가공을 하였는지는 불분명하다. Rock properties around the Namri temple site was studied source area and archaeogeological interpretations based on petrology, mineralogy and geochemistry. Excavated rocks from the area composed of granitic rocks (alkali granite, biotite granite, micrographic granite, eqigranular granite, pink feldspar granite), fine grained sedimentary rocks (shale and siltstone), hornfels, tuffaceous and basic dyke rocks. Almost rock materials of the west pagoda made of white medium grained alkali granite and biotite granite. As the magnetic susceptibility (MS), biotite granite ranges from 4.94 to 7.59 (mean 6.47), and micrographic granite is lowest value of 0.86 to 1.54 (mean 1.13). The mean MS values revealed that alkali granite = 3.51, pink feldspar granite = 5.88, eqigranular granite = 4.89, which are little variatins because of differentiation process and field occurrences. In the geochemical data, the granitic rocks represent a comagmatic calc-alkali series granite. The values of WPI and CIA for rock properties from the Namri temple site range from 49.87 to 61.64 and 4.12 to 8.46, respectively, which values don't going active weathering right now, but weathering potential represents very high. On the basis of petrology and geochemistry of the rock properties from the Namri temple site, the genetic types are the similar as those of the Namsan granite. Therefore, the using rocks of the Namri temple site suggested that domestic type rock properties getting material in the actual place and making in situ, and foreign type rock properties getting from the upstream area are coexisted. Domestic types are sure of making the actual place material, but unclear where is a quarry and working.