Stain improvement in the white button mushroom ‘Seolgang’ and its varietal characteristics in Agaricus bisporus

Byung-Joo Lee,Mi-Ae Lee,Yong-Gyun Kim,Kwang-Won Lee,Yong-Pyo Lim,Byung-Eui Lee,Ho-Yeon Song 한국버섯학회 2012 한국버섯학회지 Vol.10 No.4

The button mushroom (Agaricus bisporus) is one of the most widely cultivated important edible mushroom species. In the breeding of new button mushroom, ‘Seolgang’ was developed by crossing two monokaryons ‘CM020913-27’ and ‘SSU423-31’. Because of the secondarily homothallism, only a small percentage of the basidia produce 3 or 4 spores, which are mostly haploid (n) and do not fruit. Single spore cultures derived from these types of spores produce a vegetative mycelium that also contain a variable number of genetically identical nuclei per cell called monokaryon. The lack of clamp connections between monokaryon and dikaryon required a series of mycelial culture and fruiting test. After crossing, hybrids were cultivated on a small scale and on a commercial scale at a farm. For this, the spawn was made by a commercial spawn producer and the spawned compost by a commercial compost producer. Mycelial growth of ‘Seolgang’ on CDA was better at 20℃ and 25℃ when it was compared with that of ‘505 Ho’. The mature cap shape of new strain ‘Seolgang’ is oblate spheroid and the immature cap shape is round to oblate spheroid. The cap diameter was 41.2 mm on average. In comparison with white strain ‘505 Ho’, the strain had a yield that was 9% higher. It produced fruiting bodies which had a higher weight on average per fruiting body and were 19% firmer with a good shelf life. Days of fruiting body were 3-4 days later than those of ‘505 Ho’. The physical characteristics such as elasticity, chewiness, adhesiveness were better than that of ‘505 Ho’. Genetic analysis of the new strain ‘Seolgang’ showed different profiles compared to ‘505 Ho’, CM02913-27, SSU413-31, when RAPD primers A02 and O04 were used.

췌장염에 의한 가성낭종내에 생긴 가성동맥류 : 1례 보고

오연희,이채경,김승현,이성우,양창현,이정호,이영현 동국대학교 경주대학 1996 東國論集 Vol.15 No.-

만성 췌장염의 합병증으로 생긴 가성동맥류는 드문질환으로, 반복적으로 다량의 출혈을 일으킬 수 있으며, 치료를 하지 않을 경우 사망율이 높기 때문에, 조기 진단과 치료가 필수적이다. 저자들은 38세 남자 환자에서 췌장염의 합병증으로 생긴 가성낭종내에서 발생한 가성동맥류 1예를 경험하였기에 보고하는 바이다. 가성 동맥류의 색혈류도플러상 낭종내에 양방향 흐름의 와류를 볼 수 있었다. Pancreatic Pseudoaneurysm within Pseudocyst from Pancreatitis: 1 Case Department of Diagnostic Radiology and Internal Medicine, College of Medicine,DongGuk University Yeon Hee OH M.D., Chae Kyeong Lee M.D., Seoung Hyeon Kim M.D., Sung Woo Lee M.D., Chang Heon Yang M.D., Jung Ho Lee M.D., Young Hyun Lee M.D. Pseudoaneurysm from pancreatitis is uncommon, but it can cause recurrent and massive hemorrhage. Because of high morbidity and mortality, early detection and treatment of the pseudoaneurysm is needed. We report a case of pseudoaneurysm within pseudocyst from pancreatitis. Color-flow Doppler sonography shows bidirectional flow and turbulent arterial flow, within anechoic mass.

2003년 국내 중증급성호흡기증후군 진료 현황 및 문제점 분석

이진수,김은실,정문현,백제중,정선화,안주희,최영화,이선희,고철우,김성범,김민자,박승철,기현균,송재훈,최상호,김양수,이상오,조용균,박영훈,정숙인,김연숙,이흥범,손창희,장성희,정희진,김우주 대한감염학회 2004 감염과 화학요법 Vol.36 No.3

목적 : 2002년 말 중국에서 SARS가 발생한 이후 국내에서도 2003년 10월까지 총 3명의 추정환자, 17명의 의심환자가 보고되었다. 향후 추가적인 SARS의 유행이 우려되는 상황에서, 그간의 SARS 환자 진료에 있어서의 실질적인 준비사항, 진료 현황 등에 대한 조사를 통해 문제점을 파악하여, 향후 더 나은 대비가 될 수 있도록 개선점을 제시하고자 하였다. 재료 및 방법 : SARS로 의심되는 환자를 진료 경험이 있는 병원의료진을 대상으로 2003년 10월에 설문조사를 실시하였다. 설문에는 SARS 환자 진료 시의 실질적인 조치, 진료 현황, 병실, 응급실 및 외래에서의 격리 시설과 준비사항, 보건당국의 관리와 지원에 관한 사항을 포함하였다. 결과 : 대상이 되는 22개 병원 중 17개(17/22, 77.2%) 병원이 설문에 응하였다. SARS 환자를 위한 격리실은 응급실, 외래, 일반병실 및 중환자실에서 각각 9개(9/17, 52.9%), 5개(5/17, 29.4%), 15개(15/16, 93.7%), 4개(4/16, 25%) 병원에서 음압처리가 되어있지 않은 일인실 혹은 다인실이 사용되었고, 1개(1/16, 6.3%) 병원에서만 일반병실에서 음압격리실이 운영되었다. 입원환자의 진찰 시 개인보호구의 착용은 거의 모든 의료기관에서 이루어졌다. 보건당국에서 SARS지정병원의 시설 등을 사전에 확인한 곳은 1곳(1/12, 8.3%)이였고, 14개 병원(14/15, 93.3%)에서는 보건당국에 의뢰한 검사결과를 통하 받지 못하였다. 결론 : 의료기관에서 SARS 환자용 격리실뿐만 아니라 기존의 격리실 설비 등이 미흡하였으며, 특히 중환자실 및 외래의 준비가 더욱 부족하였다. 보건당국의 의료기관에 대한 종합적인 지원이 부족하였고, 병원과의 원활한 연계가 잘 이루어지지 않았다. SARS 만이 아닌 격리를 필요로 하는 질환의 적절한 진료를 위해 향후 병원 시설의 정비와 정부차원에서의 보다 구체적이고 실질적인 대책마련이 필요하다. Background : There was an worldwide outbreak of the Severe Acute Respiratory Syndrome (SARS) originated from China in late 2002. During that period three cases of suspected SARS and 17 cases of probable SARS were reported in Korea. With the concerns about the reemergence of SARS-coV transmission, it is important to be prepared for any possibility. So, this study is aimed to analysis the past measures in managing SARS and propose the amendatory plans to improve the preparedness. Materials & Methods : Questionnaires were collected among clinicians with any experience in managing the probable or suspected SARS cases in Oct. 2003. 17 out of 22 hospitals responded to the questionnaire. The contents in the questionnaire were practical activities, personal equipments, response plans, isolation facilities in emergency centers, outpatient clinics, general wards and intensive care units, and relationship with the public health department. Results : The dedicated isolation rooms in emergency centers, outpatient clinics, general wards, and intensive care units were prepared in 9 (9/17, 52.9%), 5 (5/17, 29.4%), 15 (15/16, 93.7%), and 4 (4/16, 25.0%) hospitals, respectively. Except for one hospital that newly made negative pressure room for SARS, single or multi-bed rooms without airborne infection control were used in all the other hospitals. The personal precaution principles were kept quite well in general wards. Before the designation of SARS hospital by the public health department prior evalution to see if the hospital was suitable for managing SARS was conducted in only 1 (1/12, 8.3%) hospital. The results of laboratory diagnosis were reported back in 1 (1/15, 6.6%) hospital. Conclusions : The isolation facilities which can control airborne infection were almost deficient not only for SARS but also for other respiratory transmissible diseases. For the infection control of transmissible diseases including SARS, more investment is needed on medical facilities and comprehensive support from the public health department required.

충남대학교 학술림 산책로 훼손실태 및 관리방안에 관한 연구

이준우,김명준,최윤호,전용준,오도교,김민호,이소연,이경형 충남대학교 농업과학연구소 2004 농업과학연구 Vol.31 No.2

This study concentrates an the trail of the CNU(Chungnam National University) forest. The amount of use and the pattern of damage are considered, as well as the extent of environmental damage. Finally, some managerial strategies are proposed in connection with sustainable maintenance. The trail of the university forest is composed of three sections; one is the neighborhood park and the Institute of Information Technology/Communications, the second is the Institute of Information Technology/Communications and the Dormitory, and the third section is the Dormitory and the Foreign prof's Apt. The total length of the trail is 2.6km. The daily amount of use is 1,269 visitors per day. The amount of using increased three times the amount of that in 1998. This study shows the pattern of damage of the trail in the order of root-exposure, widening, rock-exposure, deepening and the divergence in the frequency of occurrence of all these factors. Environmental damage is also graded thus: grade 3 is 27.4m, grade 4 is 0.8m, grade 5 is 1.5m, and grade 6 is 0.2m. Accordingly, some managerial strategies were proposed in connection with the sustainable trail maintenance.

Implication of necrosis-linked p53 aggregation in acquired apoptotic resistance to 5-FU in MCF-7 multicellular tumour spheroids

Lee, Su Yeon,Jeong, Eui-Kyong,Jeon, Hyun Min,Kim, Cho Hee,Kang, Ho Sung Spandidos Publications 2010 Oncology reports Vol.24 No.1

<P>Three-dimensional (3D) multicellular tumour spheroids (MTS) have been used as an in vitro model of solid tumours for drug resistance studies because they mimic the growth characteristics of in vivo tumours more closely than in vitro two-dimensional (2D) culture of cancer cell lines. As observed in solid tumours, MTS exhibits a proliferation gradient with outer regions consisting of proliferating cells that surround inner quiescent cells. The innermost cells in core regions undergo cell death mostly by necrosis to form necrotic core due to insufficient supply of oxygen and nutrient such as glucose with increasing size of spheroids. Tumour necrosis is thought to indicate a poor prognosis and to contribute to acquisition of chemoresistance in solid tumours; however, the mechanism underlying necrosis-mediated chemoresistance remains unclear. In this study, we examined the chemoresistance to 5-Fluorouracil (5-FU) using MCF-7 breast cancer MTS. 5-FU (400 microM) induced apoptosis in MCF-7 cell monolayer as determined by HO/PI staining, PARP cleavage, p53 induction, Bax induction, and Bcl-2 down-regulation. When MCF-7 breast tumour spheroids were cultured on agarose for 8 days, they reached approximately 700 microm in diameter, with a necrotic core. We found that 5-FU-induced apoptosis is markedly reduced in spheroids that were cultured for 9 days and had necrotic core, compared with MCF-7 monolayer cells and spheroids that were cultured for 6 days and had no necrotic core, indicating that the formation of necrotic core may be linked to acquisition of chemoresistance to 5-FU. We also found that a specific set of cellular proteins including p53 was aggregated into a RIPA-insoluble form during MTS culture. Furthermore, most of p53 induced by 5-FU was aggregated in MTS with necrotic core. Our results suggest that necrosis-linked p53 aggregation may contribute to acquired apoptotic resistance to 5-FU in MTS model system.</P>

UV/Persulfate 산화 및 전기분해 방법을 이용한 TMAH 용액 및 부산물 처리 연구

최효연 ( Hyo-yeon Choi ),이용호 ( Yong-ho Lee ),이효주 ( Hyo-joo Lee ),이수민 ( Su-min Lee ),박대원 ( Dae-won Pak ) 한국폐기물자원순환학회(구 한국폐기물학회) 2019 한국폐기물자원순환학회 춘계학술발표논문집 Vol.2019 No.-

현재 TFT-LCD를 제조하는 과정에서 다양한 종류의 화학약품을 사용하며 이로 인해 다양한 종류의 폐수가 다량 발생되고 있다. 이 중 TMAH(Tetra methyl ammonium hydroxide)는 유입되는 질소의 주요 성분으로 TFT-LCD 제조 공정에 있어서 핵심 공정 중의 하나인 Photo 공정 중 포토 레지스트(photo resist)의 현상액으로 사용되는 강알칼리의 유기 아민계 용액으로, 무색, 투명의 강염기성 물질이고, 수용성, 가연성의 성질을 나타낸다. 또한 그 농도가 증가하면서 강한 독성을 배출하여 생물학적 분해가 어렵고, 분자 구조 속에 질소를 포함하고 있어, 분해 시 암모니아성 질소를 생성하는 특성을 가지고 있다. 이에 따라 자연 수중 생태계에 영향 등의 문제가 발생되기 때문에 TMAH 폐수의 효율적인 처리방법 도출이 필요한 사항이다. 현재 TMAH를 함유한 폐수는 호기성 생분해, 혐기성 생분해, 촉매 산화, 광촉매 분해(UV 조사) 또는 이온 교환 기술 등으로 처리되고 있습니다. 그 중 UV 조사는 과황산염 활성화를 위한 에너지의 한 형태로 적용되며 난분해성 분해에 많이 적용되고 있다. 과황산음이온(S₂O₈ ^2-)은 산화환원전위가 2.01 V인 강한 산화제이며 토양과 지하수에 존재하는 유기오염물질을 분해하는데 효과적이라고 한다. 과황산염 반응 메커니즘은 다양한 과황산염 활성화를 통한 강한 황산염 라디칼(SO₄^-·) 및 하이드록실 라디칼(HO^·)의 생성에 기반을 두고 있으며 1 mole의 황산염은 자외선 조사를 통해 2 mole의 황산염 라디칼을 생성하도록 활성화 될 수 있다고 보고되어 다양한 난분해성 유기 오염 물질을 분해에 적용되고 있다. 전기화학적 산화 처리공정은 고분자량을 가지는 화합물을 파괴하며 난분해성 유기물질의 독성을 감소시킬 수 있어 효과적인 것으로 제시되고 있으며, 고도처리공정들 가운데 오염물질 생분해성과 관계없이 높은 제거율과 처리시간이 짧은 운전특성을 나타내고 있어 유기화합물 또는 질소화합물의 제거에 있어서 응용가능성이 높은 것으로 평가되고 있다. 따라서, 본 연구에서는 TMAH 모형폐수를 제조하여 먼저 과황산염(S₂O₈ ^2-)을 이용한 UV 산화방법으로 TMAH를 분해하고 그에 따른 부산물을 전기분해 방법으로 처리하고자 하였다.

The Antioxidant Effect of the Rhaponticum uniflorum Extracts on the Oxidative Stress in H₂O₂ induced Human Keratinocytes

So Hee Ahn,Won Yeoung Choi,Ji Won Jeong,Geon Woo Park,Su Yeon Kang,Eun Jeong Kim,Su Rim Sin,Eun Seo Jang,Gye Won Lee,Young Ho Cho 한국식품영양과학회 2021 한국식품영양과학회 학술대회발표집 Vol.2021 No.10

In this study, To investigate the antioxidant activities of Rhaponticum uniflorum extracts (RUE), we assessed scavenging activities on DPPH, ABTS radicals, and reducing power. The DPPH and ABTS radical scavenging activities (SC50) of RUE were 96.97±1.19 μg/mL and 31.26±0.13 μ g/mL, respectively, and the reducing power significantly increased dose-dependent manner. To study the antioxidant effecters of RUE in H₂O₂ induced human keratinocytes, expression of Nuclear factor erythroid-2-related factor 2 (Nrf2) and heme oxygenase-1 (HO-1) were determined by Western blot and qRT-PCR. RUE induced the expression of HO-1, a critical target enzyme of Nrf2, in a concentration-dependent manner. The expression of HO- 1 mRNA was also induced in H₂O₂-treated HaCaT cells but no significant difference in the level of HO-1 mRNA was observed. Taken together, these results suggest that RUE may be useful as a potential ingredient for anti-aging products.

전자상거래가 거래가격에 미치는 영향부석 : 일본 벤처기업 AUCNET의 사례를 중심으로 A Case of Venture Company for On-line Used-car Transactions

李豪根,金晟淵 한국중소기업학회 1998 기업가정신과 벤처연구 Vol.1 No.2

AUCNET is an electronic market system for used-car transactions in Japan. AUCNET was built by an entrepreneurial used-car dealer, who foresaw that the redesign of the auto auction process using computers and telecommunications would significantly improve the market efficiency of used-car trading. We have found that the quality of secondhand cars traded in AUCNET is much higher than those sold in traditional markets. Separation of car transportation from the auction process using computer and communication technologies was revolutionary to most dealers who used to come to auction sites for trading cars. To overcome resistance and suspicion over the new market process, AUCNET had to establish its institutional rule to reduce transaction risks of uncertain qualities, standardizing the product representation and inspection process to establish trust for information of cars listed in its electronic market system. As a result, the quality of secondhand cars traded in AUCNET is much higher than those sold in traditional markets and this difference in car quality is a prime reason for the higher vehicle price in AUCNET. AUCNET's success provides a significant implication to venture business for electronic commerce. If qualities of products traded over electronic networks widely vary, the venture firm should establish institutional rules and policies so that market participants can trust product information in electronic markets.

흉추의 병적골절을 보인 심한 신성 골이영양증 1례 : 증례 보고

이송주,이은미,임학,정연순,장재호 고신대학교의과대학 2008 고신대학교 의과대학 학술지 Vol.23 No.4

A 33-year-old woman presented with tingling sensation and weakness in the both hands and feet. She had a diagnosis of secondary hyperparathyroidism caused by renal failure. Her height was shortened due to severe kyphosis. On plain radiograph, there were fractures on both femoral necks and pathologic fracture on thoracic vertebra. An MRI scan of the spine demonstrated a expansile, heterogeneous hypointense lesion of the 2nd thoracic spine, which remained hypointense on T2 images. Following total parathyroidectomy the patient underwent a thoracic decompression and spinal stabilization from the back. Hyperparathyroidism may cause brown tumor and tumor associated pathologic fracture in the spine. Involvement of the spine with neural compression is extremely rare. An early spinal decompression with parathyroidectomy leads to remission of symptoms.

개선된 중성염 전해공정을 이용한 모의 방사성금속폐기물의 제염

이지훈,육완이,양호연,하종현 대한방사선 방어학회 2002 방사선방어학회지 Vol.27 No.2

원자력발전소에서 주로 발생되는 금속폐기물인 탄소강을 중성염전해질인 1.7M의 황산나트륨(Na₂SO₄)과 질산나트륨(NaNO₃)을 이용하여 기존전해제염과 개선된 전해제염공정의 비교실험을 수행하였다. 양극은 인코넬, 음극은 티타늄으로 하여 상온에서 1시간동안 반응시켜 금속폐기물 모재의 weight loss, 두께변화, 전해질 내 침전물농도, SEM을 이용하여 제염전후의 금속폐기물 표면의 형상을 분석하였다. 실험결과 개선된 전해제염 적용시 전해질 종류별 전류밀도 변화에 따른 실험에서는 전류밀도가 0.1∼0.6A/cm²으로 증가함에 따라 1.7M의 황산나트륨 적용시 금속폐기물 모재의 두께변화는 0.48±0.005∼67.7±0.02um, 1.7M의 질산나트륨 적용시에는 0.06±0.005∼17.7±0.05로 나타나 같은 전류밀도에서 황산나트륨 적용시 금속폐기물 모재의 표면 제염효율이 더욱 높은 양상을 보였다. 또한 전류밀도 0.3 A/cm² 및 1.7M의 황산나트륨의 조건에서 개선된 전해제염 적용 시 9.8±0.01um의 금속폐기물 두께변화를 보여 기존전해제염 적용시인 3.7±0.03um의 금속폐기물 두께변화보다 2배 이상의 표면 제염효과를 보였다. Conventional and modified electrolytic decontamination experiment were performed in the 1.7 M solution of sodium sulfate and sodium nitrate for decontamination of carbon steel as the simulated metal wastes which have been produced in large amounts from nuclear power plants. Anode and cathode were used as inconel and titanium respectively. The reaction time and temperature were 1 hr and 25℃. The analyses were performed of the characteristics such as weight loss and thickness change of metal waste, suspended solid in electrolyte and SEM observation. In modified electrolyte decontamination system with increased current density ranged from 0.1 to 0.6 A/cm², the metal waste showed thickness changes of 0.48±0.005 to 67.7±0.02um in 1.7 M sodium sulfate and those of 0.06±0.005 to 17.7±0.05um in sodium nitrate. Metal waste in modified electrolyte decontamination system showed the thickness change of 9.8±0.01um while it reacted up to 3.7±0.03um in conventional system with 0.3 A/cm² of current density and 1.7 M sodium sulfate. Decontamination efficiencies of modified electrolytic process are much higher than that of conventional electrolytic process when both are applied to metal waste.