Anti-Inflammatory Activity of Questinol Isolated from Marine-Derived Fungus Eurotium amstelodami in Lipopolysaccharide-Stimulated RAW 264.7 Macrophages

( Xiu Dong Yang ),( Min Cheol Kang ),( Yong Li ),( Eun A Kim ),( Sung Myung Kang ),( You Jin Jeon ) 한국미생물 · 생명공학회 2014 Journal of microbiology and biotechnology Vol.24 No.10

In the present study, an anthraquinone derivative, questinol was successfully isolated from the broth extract of the marine-derived fungus Eurotium amstelodami for the first time. The structure of questinol was determined based on the analysis of the MS and NMR spectral data as well as comparison of those data with the published data. Moreover, the anti-inflammatory effect of questinol in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells was investigated. The results showed that questinol did not exhibit cytotoxicity in LPS-stimulated RAW 264.7 cells up to 200 μM. Questinol could significantly inhibit NO and PGE2 production at indicated concentrations. Questinol was also found to inhibit the production of pro-inflammatory cytokines, including TNF-α, IL-1β, and IL-6. Furthermore, the western blot analysis showed that questinol suppressed the expression level of iNOS in a dose-dependent manner. However, questinol could slightly inhibit the expression of COX-2 at the concentration of 200 μM. Therefore, our study suggests that questinol might be selected as a promising agent for the prevention and therapy of inflammatory disease.

Determination of Protein, Fat, Starch, and Amino Acids in Foxtail Millet [Setaria italica (L.) Beauv.] by Fourier Transform Near-Infrared Reflectance Spectroscopy

Xiu-Shi Yang,Li-Li Wang,Xian-Rong Zhou,Shaomin Shuang,Zhi-Hua Zhu,Nan Li,Yan Li,Fang Liu,San-Cai Liu,Ping Lu,Guixing Ren,Chuan Dong 한국식품과학회 2013 Food Science and Biotechnology Vol.22 No.6

Quantitative detection of protein, fat, starch,and amino acids in foxtail millet using Fourier transformnear-infrared spectroscopy (NIRS) was investigated. Foxtail millet samples (n=259) were analyzed using NIRS. Spectral data were linearized with data from chemicalanalyses. Calibration models were established using apartial least-squares (PLS) algorithm with cross-validation. Optimized models were tested using external validation setsamples with coefficients of determination in the externalvalidation (R2val) of >0.90. Residual predictive deviation(RPD) values were nearly equal to or >2.5 for crudeprotein, alanine, aspartic acid, glutamic acid, isoleucine,leucine, and serine. However, for glycine, histidine,phenylalanine, proline, threonine, tyrosine, and valine, theR2val values were >0.83 and RPD values were nearly equalto or >2.0. For crude fat, total starch, arginine, and lysine,the R2val values were >0.70 and RPD values were >1.5. NIRS is a rapid determination tool for foxtail milletbreeding, and for quality control.

Nanotherapeutics with immunoregulatory functions for the treatment of bacterial infection

Dongliang Yang,Meng Ding,Yanni Song,Yanling Hu,Weijun Xiu,Lihui Yuwen,Yannan Xie,Yingnan Song,Jinjun Shao,Xuejiao Song,Heng Dong 한국생체재료학회 2023 생체재료학회지 Vol.27 No.00

The advent of drug-resistant pathogens results in the occurrence of stubborn bacterial infections that cannot betreated with traditional antibiotics. Antibacterial immunotherapy by reviving or activating the body’s immune systemto eliminate pathogenic bacteria has confrmed promising therapeutic strategies in controlling bacterial infections. Subsequent studies found that antimicrobial immunotherapy has its own benefts and limitations, such as avoidingrecurrence of infection and autoimmunity-induced side efects. Current studies indicate that the various antibacterialtherapeutic strategies inducing immune regulation can achieve superior therapeutic efcacy compared with mono therapy alone. Therefore, summarizing the recent advances in nanomedicine with immunomodulatory functionsfor combating bacterial infections is necessary. Herein, we briefy introduce the crisis caused by drug-resistantbacteria and the opportunity for antibacterial immunotherapy. Then, immune-involved multimodal antibacterialtherapy for the treatment of infectious diseases was systematically summarized. Finally, the prospects and challengesof immune-involved combinational therapy are discussed.

Disturbance-observer-based Neural Sliding Mode Repetitive Learning Control of Hydraulic Rehabilitation Exoskeleton Knee Joint with Input Saturation

Yong Yang,Xiu-Cheng Dong,Zu-Quan Wu,Xia Liu,De-Qing Huang 제어·로봇·시스템학회 2022 International Journal of Control, Automation, and Vol.20 No.12

Rehabilitation exoskeleton is a wearable robot for recovery training of stroke patients. It is a complex human-robot interaction system with highly nonlinearities, such as modeling uncertainties, unknown human-robot interactive force, input constraints, and external disturbances. This paper focuses on trajectory tracking control of a rehabilitation exoskeleton knee joint which is driven by a hydraulic actuator with input saturation. A radial basis function neural network (RBF-NN) sliding mode repetitive learning control strategy is presented for the exoskeleton knee joint, where the RBF-NN is combined with a sliding mode surface to compensate for the modeling uncertainties and the controller difference as well as enhanced the robustness of the system. Incorporating with a nonlinear observer, a repetitive learning scheme is constructed to estimate the unknown external disturbances and learn the periodic human-robot interactive force caused by repetitive recovery training. Utilizing the Lyapunov approach, the stability of the closed-loop control system and the observer are guaranteed. Comparative simulation results verify the effectiveness of the proposed control scheme.

Reaction force of ship stern bearing in hull large deformation based on stochastic theory

Zhang, Sheng-dong,Long, Zhi-lin,Yang, Xiu-ying The Society of Naval Architects of Korea 2020 International Journal of Naval Architecture and Oc Vol.12 No.1

A theoretical calculation model for ship stern bearings with large hull deformation is established and validated theoretically and experimentally. A hull simulation model is established to calculate hull deformations corresponding to the reaction force of stern bearings under multi-factor and multi-operating conditions. The results show that in the condition of wave load, hull deformation shows randomness; the aft stern tube bearing load obeys the Gaussian distribution and its value increases significantly compared with the load under static, and the probability of aft stern tube bearing load greater than 1 is 65.7%. The influence laws and levels between hull deformation and bearing reaction force are revealed, and suggestions for ship stern bearing specifications are proffered accordingly.

Catalytic topological insulator Bi₂Se₃ nanoparticles for <i>in</i> <i>vivo</i> protection against ionizing radiation

Zhang, Xiao-Dong,Jing, Yaqi,Song, Shasha,Yang, Jiang,Wang, Jun-Ying,Xue, Xuhui,Min, Yuho,Park, Gyeongbae,Shen, Xiu,Sun, Yuan-Ming,Jeong, Unyong Elsevier 2017 Nanomedicine Vol.13 No.5

<P><B>Abstract</B></P> <P>Bi<SUB>2</SUB>Se<SUB>3</SUB> nanoparticles (NPs) have attracted wide interests in biological and medical applications. Layer-like Bi<SUB>2</SUB>Se<SUB>3</SUB> with high active surface area is promising for free radical scavenging. Here, we extended the medical applications of Bi<SUB>2</SUB>Se<SUB>3</SUB> NPs further to <I>in vivo</I> protection against ionizing radiation based on their superior antioxidant activities and electrocatalytic properties. It was found that Bi<SUB>2</SUB>Se<SUB>3</SUB> NPs can significantly increase the surviving fraction of mice after exposure of high-energy radiation of gamma ray. Additionally, the Bi<SUB>2</SUB>Se<SUB>3</SUB> NPs can help to recover radiation-lowered red blood cell counts, white blood cell counts and platelet levels. Further investigations revealed that Bi<SUB>2</SUB>Se<SUB>3</SUB> NPs behaved as functional free radical scavengers and significantly decreased the level of methylenedioxyamphetamine. <I>In vivo</I> toxicity studies showed that Bi<SUB>2</SUB>Se<SUB>3</SUB> NPs did not cause significant side effects in panels of blood chemistry, clinical biochemistry and pathology.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Bi<SUB>2</SUB>Se<SUB>3</SUB> NPs can significantly increase the surviving fraction of mice up to 70% after Gamma radiation. </LI> <LI> Bi<SUB>2</SUB>Se<SUB>3</SUB> NPs can help to recover radiation-lowered red blood cell counts, white blood cell counts and platelet levels. </LI> <LI> Bi<SUB>2</SUB>Se<SUB>3</SUB> NPs behaved as functional free radical scavengers and significantly decreased the level of methylenedioxyamphetamine. </LI> </UL> </P> <P><B>Graphical Abstract</B></P> <P>Layer-like Bi<SUB>2</SUB>Se<SUB>3</SUB> with high active surface area can protect mice against ionizing radiation based on their superior antioxidant activities and electrocatalytic properties. Bi<SUB>2</SUB>Se<SUB>3</SUB> NPs can significantly increase the surviving fraction of mice up to 70% after exposure of high-energy radiation of gamma ray. Bi<SUB>2</SUB>Se<SUB>3</SUB> NPs behaved as functional free radical scavengers and significantly decreased the level of methylenedioxyamphetamine. <I>In vivo</I> toxicity studies showed that Bi<SUB>2</SUB>Se<SUB>3</SUB> NPs did not cause significant side effects in panels of blood chemistry, clinical biochemistry and pathology.</P> <P>[DISPLAY OMISSION]</P>

Does a High Circulating Tumor Cell Count in Portal Vein Predict the Involvement of Portal System in Pancreatic Cancer Patients?

ZHAO Xudong,DONG Xiu,MA Yongsu,GAO Hongqiao,TIAN Xiaodong,ZHAO Xiaohang,YANG Yinmo 대한외과학회 2018 대한외과학회 학술대회 초록집 Vol.2018 No.11

An Overview of Remote Sensing of Chlorophyll Fluorescence

Xiao-Gang Xing,Dong-Zhi Zhao,Yu-Guang Liu,Jian-Hong Yang,Peng Xiu,Lin Wang 한국해양과학기술원 2007 Ocean science journal Vol.42 No.1

Besides empirical algorithms with the blue-green ratio, the algorithms based on fluorescence are also important and valid methods for retrieving chlorophyll-a concentration in the ocean waters, especially for Case II waters and the sea with algal blooming. This study reviews the history of initial cognitions, investigations and detailed approaches towards chlorophyll fluorescence, and then introduces the biological mechanism of fluorescence remote sensing and main spectral characteristics such as the positive correlation between fluorescence and chlorophyll concentration, the red shift phenomena. Meanwhile, there exist many influence factors that increase complexity of fluorescence remote sensing, such as fluorescence quantum yield, physiological status of various algae, substances with related optical property in the ocean, atmospheric absorption etc. Based on these cognitions, scientists have found two ways to calculate the amount of fluorescence detected by ocean color sensors: fluorescence line height and reflectance ratio. These two ways are currently the foundation for retrieval of chlorophyll-a concentration in the ocean. As the in-situ measurements and synchronous satellite data are continuously being accumulated, the fluorescence remote sensing of chlorophyll-a concentration in Case II waters should be recognized more thoroughly and new algorithms could be expected.

Associations Between Infiltrating Lymphocyte Subsets and Hepatocellular Carcinoma

Guo, Cun-Li,Yang, Hai-Chao,Yang, Xiu-Hua,Cheng, Wen,Dong, Tian-Xiu,Zhu, Wen-Jing,Xu, Zheng,Zhao, Liang Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.11

Aims: We aimed to analyze the phenotype of tumor-infiltrating lymphocytes (TILs) and non-tumor infiltrating lymphocytes (NILs) in HCC and non-tumor tissues, and evaluate relationships between changes in these cells and the prognosis of HCC. Methods: Lymphocytes were isolated from HCC and corresponding non-tumor tissues and tested by flow cytometry. For comparison, clinical parameters were analyzed. Results: Compared with the non-tumor tissue, tumor tissue had a lower intensity of NK, NKT andCD8+T cell infiltration. TILs had higher intensity of CD4+CD25+Foxp3+regulatory T cell (Treg cells) infiltration compared with that in NILs. The prevalence of Treg cells was associated with fewer CD8 + T lymphocytes in the HCC immune microenvironment. The frequencies of NK cells and CD8+T cells in TILs of HCC patients with metastasis less than 12 months were lower than those without metastasis. However, the frequency of Treg cells was higher than those without metastasis. Conclusion: These results suggest that the frequencies of CD8+T, NK and NKT cells as well as Treg cells in the tumor tissue of HCC are significantly associated with patient survival, and could be applied as predictive indicators for HCC prognosis.

An Overview of Remote Sensing of Chlorophyll Fluorescence

Xing, Xiao-Gang,Zhao, Dong-Zhi,Liu, Yu-Guang,Yang, Jian-Hong,Xiu, Peng,Wang, Lin The Korean Society of Oceanography 2007 Ocean science journal Vol.42 No.1

Besides empirical algorithms with the blue-green ratio, the algorithms based on fluorescence are also important and valid methods for retrieving chlorophyll-a concentration in the ocean waters, especially for Case II waters and the sea with algal blooming. This study reviews the history of initial cognitions, investigations and detailed approaches towards chlorophyll fluorescence, and then introduces the biological mechanism of fluorescence remote sensing and main spectral characteristics such as the positive correlation between fluorescence and chlorophyll concentration, the red shift phenomena. Meanwhile, there exist many influence factors that increase complexity of fluorescence remote sensing, such as fluorescence quantum yield, physiological status of various algae, substances with related optical property in the ocean, atmospheric absorption etc. Based on these cognitions, scientists have found two ways to calculate the amount of fluorescence detected by ocean color sensors: fluorescence line height and reflectance ratio. These two ways are currently the foundation for retrieval of chlorophyll-a concentration in the ocean. As the in-situ measurements and synchronous satellite data are continuously being accumulated, the fluorescence remote sensing of chlorophyll-a concentration in Case II waters should be recognized more thoroughly and new algorithms could be expected.