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( Xiang Yang Ge ),( Yan Xu ),( Xiang Chen ),( Long Yun Zhang ) 한국미생물 · 생명공학회 2015 Journal of microbiology and biotechnology Vol.25 No.1
This study describes a novel strategy to regulate the metabolic flux for lactic acid production in Lactobacillus casei. The ldhL gene encoding L-lactate dehydrogenase (L-LDH) was overexpressed in L. casei, and a two-stage oxygen supply strategy (TOS) that maintained a medium oxygen supply level during the early fermentation phase, and a low oxygen supply level in the later phase was carried out. As a consequence, a maximum L-LDH activity of 95.6 U/ml was obtained in the recombinant strain, which was over 4-fold higher than that of the initial strain. Under the TOS for L. casei (pMG-ldhL), the maximum lactic acid concentration of 159.6 g/l was obtained in 36 h, corresponding to a 62.8% increase. The results presented here provide a novel way to regulate the metabolic flux of L. casei for lactic acid production in different fermentation stages, which is available to enhance organic acid production in other strains.
AFSO: An Adaptative Frame Size Optimization Mechanism for 802.11 Networks
( Xiaohu Ge ),( Cheng-xiang Wang ),( Yang Yang ),( Lei Shu ),( Chuang Liu ),( Lin Xiang ) 한국인터넷정보학회 2010 KSII Transactions on Internet and Information Syst Vol.4 No.3
In this paper, we analyze the impact of different frame types on self-similarity and burstiness characteristics of the aggregated frame traffic from a real 802.11 wireless local area network. We find that characteristics of aggregated frame traffic are affected by both mean frame size and the proportion of specified frame types. Based on this new knowledge, an adaptative frame size optimization (AFSO) mechanism is proposed to improve the transmission efficiency by adaptively adjusting data frame size according to the proportions of different frame types. Simulation results show that our proposed mechanism can effectively regulate the burstiness of aggregated frame traffic and improve the successful delivery rate of data frames when a fixed throughput target is set for 802.11 wireless networks.
Investigation on flutter mechanism of long-span bridges with 2d-3DOF method
Yang, Yongxin,Ge, Yaojun,Xiang, Haifan Techno-Press 2007 Wind and Structures, An International Journal (WAS Vol.10 No.5
A two-dimensional flutter analysis method (2d-3DOF method) was developed to simultaneously investigate the relationship between oscillation parameters and aerodynamic derivatives of three degrees of freedom, and to clarify the coupling effects of different degrees of freedom in flutter instability. With this method, the flutter mechanism of two typical bridge deck sections, box girder section and two-isolated-girder section, were numerically investigated, and both differences and common ground in these two typical flutter phenomena are summarized. Then the flutter stabilization effect and its mechanism for long-span bridges with box girders by using central-slotting were studied by experimental investigation of aerodynamic stability and theoretical analysis of stabilizing mechanism. Possible explanation of new findings in the evaluation trend of critical wind speed through central vent width is finally presented.
Investigation on flutter mechanism of long-span bridges with 2d-3DOF method
Yaojun Ge,Haifan Xiang,Yongxin Yang 한국풍공학회 2007 Wind and Structures, An International Journal (WAS Vol.10 No.5
A two-dimensional flutter analysis method (2d-3DOF method) was developed to simultaneously investigate the relationship between oscillation parameters and aerodynamic derivatives of three degrees of freedom, and to clarify the coupling effects of different degrees of freedom in flutter instability. With this method, the flutter mechanism of two typical bridge deck sections, box girder section and two-isolated-girder section, were numerically investigated, and both differences and common ground in these two typical flutter phenomena are summarized. Then the flutter stabilization effect and its mechanism for long-span bridges with box girders by using central-slotting were studied by experimental investigation of aerodynamic stability and theoretical analysis of stabilizing mechanism. Possible explanation of new findings in the evaluation trend of critical wind speed through central vent width is finally presented.
Inhibition Effects of Pulp on Seed Germination of American Ginseng
Yao Ge Huang(황요각),Xiang Gao Li(이향고),Shu Yu Cui(최수옥),Ji Xiang Yang(양계상),Ren Song Liu(류인송),Hack Seang Kim(김학성) 한국약용작물학회 1997 한국약용작물학회지 Vol.5 No.2
The germination inhibitory effects of American ginseng (Panax quinquefolium Linne) pulp were discussed. The germination inhibitory effects of pulp juice were decreased in a concentration de-pendent manner. When the pulp juice was diluted 0 (original juice), 1, 2, 5, 10, 20, 50 and 100 times, the radicle lengths of the assay plant, Chinese cabbage (Brassica chinensis Linne), showed 0, 0.32, 0.72, 3.13, 4.83, 16.07, 16.73 and 23.50 mm, respectively (CK=25.98 mm) The pulp evidently inhibited the embryo growth in natural fruit. The longer was the duration that the pulp stayed around the seed, the longer was the time course needed for embryo getting free from the inhibitory effects of pulp. When the depulping was performed on the day 0, 15, 30 and 60 after harvest, the time courses needed for embryo extricating the residual inhibitory effects from pulp were 30, 75, 135 and 135 days, respectively. Moreov-er, if the pulp stayed around the seed with time, that would make the seed rotten ratio increase. When the pulp stayed around the seed for 0, 15, 30, 60 and 270 days, the seed rotten ratios were 5.47, 5.71, 19.05, 27.14 and 33.33%, respectively. Therefore, we concluded that the pulp could be included in the inhibitory components which made American ginseng seed get into dormancy.
Huang, Yao-Ge,Li, Xiang-Gao,Yang, Ji-Xiang,Kuang, Ya-Lan,Yan, Jie-Kun,Cui, Shu-Yu,Liu, Ren-Song,Kim, Hack-Seang The Korean Society of Ginseng 1996 Journal of Ginseng Research Vol.20 No.3
Dynamic parameters of physiology including embryo ratio (ER), embryo length to endosperm length, dry weight ratio between embryo and endosperm (DWR) and respiration rate (RR) in American send (Panax quinquefolium L.) seed were investigated. According to the changes of ER during seed stratification, the duration of embryo afterripening could be divided into three stages as \circled1 embryo slow growth stage (ESGS), ER increased from 7.31% to 20.48% (0.16% day-1): \circled2 embryo rapid growth stage (ERGS), ER increased to 80.98% (0.61% day-1) (75G5+ ERGS=morphological afterripening stage (MP,5)) and \circled3pysiological afterripening stage (PAS), ER Increased to 88.50% (0.094 day-1) only. DWR Increased slowly from 0.20% to 2.76clp (0.016% day-1) in MAS and rapidly to 8.81% (0.061% day-1) in PAS. The RR correlated significantly with ER as well (r=0.8934 > rot, 0.6610). The steep increment of both DWR and RR in PAS indicated that the PAS was not a static stage although the ER was not changed too much. All of these may provide some information for understanding the dormancy mechanisms of American sting seed.
충적과정중 미국인삼 종자의 생리 및 생화하적 지표에 관한 동태학적 연구 III
Huang, Yao-Ge,Li, Xiang-Gao,Kuang, Ya-Lan,Yan, Jie-Kun,Cui, Shu-Yu,Yu, Wen-Bo,Yang, Ji-Xiang,Liu, Ren-Song,Kim, Hack-Seang 고려인삼학회 1997 Journal of Ginseng Research Vol.21 No.1
Dynamic parameters of biochemistry including the POD (peroxidase) activity, contents of DNA and RNA, isozymes of POD and ES (esterase) in American send (Panax quinquefolium L.) seed are reported in the present paper. The dynamic changes of POD activity proved that the PAS (physiological afterripening stage) is a stage in which some substances are prepared for seed germination. The POD activity correlated with ER (embryo ratio) significantly DNA content changed little only within 0.0036∼0.013 mg/ml, which did not correlate with ER. RNA content changed from 0.1539 to 1.0313 mg/ml and correlated significantly with RE during all of the embryo afterripening. None of the POD isozyme band was obtained in ESGS (embryo slowly growth stage), but five bands in ERGS (embryo rapidly growth stage) and six bands in PAS. Four bands of ES isozymes were obtained in ESGS, but six bands in ERGS, particularly, the content of ES isozymes increased in PAS. All of these may provide some information for understanding the dormancy mechanisms of American song seed.