Growth Inhibition of Colon Cancer through Inactivation of STAT3 Pathway by IL-10 and IL-1ra Released from Murine Macrophage

Jin Tae Hong, Dohee Won1, Mi Hee Park, Sun Mi Kown, Miran Jo, Sang-Yoon Nam, Beom Jun Lee, Young Won Yun, Ki-Wan Oh, Sang Bae Han 충북대학교 동물의학연구소 2011 Journal of Biomedical and Translational Research Vol.12 No.4

The objective of this study was to determine the effect of macrophages on growth of human colon cancer cells. The results showed that co-culture of colon cancer cells with macrophages inhibited the growth of colon cancer cells (HCT116 and SW620) depending on the number of macrophages, RAW 264.7 cells, and activated THP-1 cells accompanied by down regulation of pSTAT3 in cancer cells. We also found that expression and release of cancer cell growth inhibitory cytokines, IL-1 receptor antagonist (IL-1ra) and IL-10, was increased in macrophages. Blocking of the STAT3 pathway with specific inhibitor and siRNA of STAT3 abolished the growth of colon cancer cells and expression of IL-1ra and IL-10. In addition, neutralization of IL-1ra and IL-10 with antibodies resulted in reversal of macrophage-induced inhibition of cancer cell growth. These data showed that IL-1ra and IL-10 released from macrophages inhibit growth of colon cancer cells through inhibition of the STAT3 pathway

Flavobacterium meningosepticum에 의한 균혈증 1예

장성원,유진홍,진승원,김선우,김연식,박순민,이대훈,최민호,최주연,신완식,강문원,강지민 대한화학요법학회 1997 대한화학요법학회지 Vol.15 No.1

1996년 6월 카톨릭대학교 부천성가병원에 뇌경색으로 입원했던 61세 남자 환자의 혈액에서 Flavobacterium meningosepticum을 분리하였다. 환자는 폐렴을 동반한 패혈증의 예로 ceftriaxone과 metronidazole을 투여하였으나 입원 48시간만에 사망하였다. Flabovacterium meningosepticum is a rae cause of nosocomial infection which shows multi-drug resistance. It mainly invades patients with impaired immunity. Recently, we experienced a case of F. meningosepticum bacteremia in a patient with chronic debilitated state owing to stroke. The clinical progress was so rapid that he died within 48 hours. Blood culture revealed F. meningosepticum which showed characteristic yellow colony in blood agar plate.

급성 골수성 백혈병 환자에서 관해 유도 항암 화학요법 후 발생한 베르니케 뇌증

황진원,정재현,서광원,최병진,송영진,김종윤,이은지,임홍규,이상민 인제대학교 2009 仁濟醫學 Vol.30 No.-

Wernicke's encephalopathy is characterized by the triad of ocular symptoms, ataxia, and mental confusion and neuropsychiatric condition generally caused by acute thiamine deficiency. Although it is common in the severe alcoholics, several other causes also have been identified, such as total parenteral nutrition (TPN) use, hyperemesis gravidarum, anorexia nervosa, hemodialysis, uremia, and acquired immunodeficiency syndrome(AIDS). Chemotherapy in patient with malignanacy and long term total parenteral nutrition following bone marrow transplantation are related with Wernicke's encephalopathy. A 48-year-old woman had been treated with Daunorubicin and Cytarabine for remission induction chemotherapy of acute myeloblastic leukemia and intravenous hyperalimentation due to persistent hematochezia and ileus caused by infiltration of leukemic cell in terminal ileum. She suddenly complained of diplopia, tremor, and mental confusion at 32th day after chemotherapy and generalized tonic-clonic seizure at 34th day after chemotherapy. The diagnosis of Wernicke's encephalopathy was made with clinical presentations and brain MRI. Brain MR T2-weighted & Flair image revealed a high signal intensity lesions bilaterally at the medial thalamus and peri-acueductal grey matter of the midbrain. The patient was successfully treated with vitamin B1. We presented a case of acute Wernicke's encephalopathy developed after remission induction chemotherapy followed by intravenous hyperalirnentation in a patient with acute myeloblastic leukemia.

배구 서브리시브에 따른 팀 간 공격 차이 분석

김종원 ( Jong Won Kim ),김주학 ( Joo Hak Kim ),김진환 ( Jin Hwan Kim ),홍성진 ( Seong Jin Hong ) 한국스포츠정책과학원(구 한국스포츠개발원) 2011 체육과학연구 Vol.22 No.3

이 연구는 국내 남자프로배구경기에서 나타난 서브리시브에 따른 팀간 공격 차이를 서브리시브의 유효성, 세터/비세터에 의한 세트, 세터의 위치, 공격형태, 공격결과를 기반으로 어떠한 차이가 있는지를 알아보기 위하여 NH농협 2009~2010 V리그 남자프로배구경기 126경기를 대상으로 하였다. 분석된 요인들을 기반으로 팀간 공격형태별 빈도, 대전팀간 교차분석, 공격형태별 성공 비율 차이를 분석하여 다음과 같이 나타났다. 첫째, 요인에 따른 공격형태별 빈도를 분석한 결과, 서브리시브가 성공하였을 경우 7개팀 중 5개팀이 속공공격, 실패하였을 경우 7개팀 모두가 오픈공격의 빈도가 높게 나타났다. 둘째, 요인에 따른 대전팀 교차분석의 차이를 검증해 본 결과 차이가 나타났다. 시즌 1위팀인 S팀은 7위팀인 A팀보다 서브리시브 성공률에서 10%이상의 차이가 나타났으며, 서브리시브의 성공·실패와 상관없이 7개팀 모두 96%이상의 세트가 세터에 의해 이루어지는 것으로 나타났다. 또한, 공격결과 성공률 평균에서는 상위팀인 S팀·H팀(56.9%), 중위팀인 D팀·L팀(54.7%), 하위팀인 W팀·K팀·A팀(47.7%)로 나타나 상위팀일수록 더 높은 공격결과 성공률 평균이 나타났다. 셋째, 공격형태별 성공 비율 차이를 분석한 결과, 이동공격을 제외한 모든 공격형태에서 차이가 나타났다. 오픈공격에서는 S팀이 D팀은 제외한 모든 팀보다 높은 오픈공격 성공 비율의 차이가 나타났으며, A팀은 W팀을 제외한 모든 팀에게 높은 오픈공격 성공 비율의 차이를 나타내주는 특징을 보였다. 시간차공격에서는 K팀이 A팀을 제외한 모든 팀에게 높은 시간차공격 성공 비율의 차이를 나타내주었으며, 속공공격에서는 H팀이 D팀을 제외한 모든 팀에게 높은 속공공격 성공 비율의 차이가 나타났다. 후위공격에서는 하위팀인 W팀·K팀·A팀에서 상위팀들에게 높은 후위공격의 성공 비율의 차이를 나타내주는 특징을 보였다. 퀵오픈공격에서는 A팀이 K팀은 제외한 모든 팀에게 높은 퀵오픈공격 성공 비율을 나타내주는 특징을 보였다. 서브리시브에 따른 팀 간 공격에서 서브리시브의 유효성, 세터/비세터에 의한 세트, 세터의 위치, 공격형태, 공격결과에 따라 팀 간 차이가 나타났다. 안정된 서브리시브와 세트는 여러 공격형태와 높은 공격성공률과의 관계가 매우 높다고 볼 수 있다. 대부분의 기록에서 승리한 팀이 패배한 팀보다, 시즌 순위 기록으로 상위팀이 하위팀보다 우위를 나타내 여러 요인들의 안정 및 높은 성공률을 위하여 꾸준한 연습과 대전팀의 경기기록에 대한 연구가 필요하다. The study was to compare the differences of attacking patterns related to serve receives basing on effectiveness of serve receives, setter/non-setter, location of setter, type of attacks and outcomes of attacks. The subjects were 126 games in 2009~2010 Korean NH Nonghyub V-league men`s championships. The analysed factors were considered on frequencies of type of attacks by each team, cross comparisons between teams played and a ratio of successful outcome by the type of attacks. The results were found as following belows; Firstly, in the comparisons of frequencies on the type of attacks, 5 teams among 7 teams shown greater frequencies on counter-attacks while the teams were winning. Secondly, there were significant differences of types of attacks between teams. The championships` winner, team S was greater than other teams about 10 % of successful serve-receives and 96% of sets were done by setter without the relationships of successful or unsuccessful serve-receives. Also, the successful percentage of attacking outcomes were ordered by ranks such as superior team S and H (56.9%), mid-ranks team D and L (54.7%) and inferior team W, K and A (47.7%). Thirdly, in the comparisons of successful ratio of type of attacks, there were significant differences between the type of attacks except moving-attacks. In the open-attacks, the ratio by team S was greater than team D. In addition, there was significant difference on the ratio by team A excepted the comparing with team W. In the time difference attacks, the ratio by team K was different comparing to other teams excepting team A. In the counter-attacks, team H were greater ratio excepting to team D. In the backward-attacks, the inferior teams (W, K and A) made greater successful ratio of attacks against the superior teams. In the quick-attacks, there was grater ratio by team A against others excepted to team K. There were differences of the effectiveness of serve-receives, sets by setter/ non-setter, location of setter, type of attacks and outcome of attacks depending on the teams. The stable serve-receives and sets were highly relevant to the various types of attacks and greater ratio of successful attacks. The continuous practice and study on oppositional data are required in order to enhance their performances because the winning and superior teams were stable ratio of successful attacks rather than the losing and inferior teams.

저작근 골막하 박리를 이용한 만성 재발성 탈구의 치험례

심재환,김기홍,문진석,민병진,이원학,홍광진 大韓顎顔面成形再建外科學會 2003 Maxillofacial Plastic Reconstructive Surgery Vol.25 No.3

Chronic recurrent dislocation(CRD) of the jaw has various etilogical causes, including trauma, congenital or acquired neurological illness, ingestion of certain medicines, incorrdination of the neuromuscular astivity of the chewing muscles, articular anatomical alteration, etc. Various surgical methods have been advocated for the treatment of the CRD. They may be divided into two groups: procedures designed to limit translation of the condyle and procedures eliminating obstacles in the condylar path. Hypomobility of mandible can be developed after BSSRO and it is probably caused by scar contracture, atrophy, and myofibrosis. Many authors, therefore, emphasized the importance of physical therapy especially after BSSRO to prevent the complication. Based on that idea, we designed more conservative surgical method to limit mandibular openning compared the methods that previously introduced and tried to treat the CRD. This new method consists of extensive subperiosteal dissection of masseter and the temporalis muscle under local anesthesia and use of intermaxillary elastics for some periods.

야뇨증 환아들의 심리사회적 특성에 대한 다기관 연구 : 행동 및 정서 문제를 중심으로

조수철,김재원,신민섭,황준원,한상원,박관현,이상돈,김경도,김건석,서홍진,이유식,정재용,김영균,문두건,남궁미경,한창희,조원열,김영식,배기수,이종국,정우영,신의진 大韓神經精神醫學會 2005 신경정신의학 Vol.44 No.6

Objectives : The aim of this study was to examine the behavioral and emotional problems associated with nocturnal enuresis in Korean children. Methods : Three hundred eighteen children with nocturnal enuresis, together with their parents, completed the Child Behavior Checklist (CBCL), Disruptive Behavior Disorder Scale according to DSM-IV (DBDS), Children's Depression Inventory (CDI), State-Trait Anxiety Inventory for Children (STAIC), and Piers-Harris Children's Self-Concept Scale (PHCSC). Ninety-three normal students were selected as the control group. Results : Compared to the normal control group, the mean scores with regard to the withdrawn, social problems, attention problems, delinquent behavior, aggressive behavior, externalizing problems and total problems profiles were significantly higher in the nocturnal enuresis group according to the CBCL results. The nocturnal enuresis group also scored significantly higher in the ADHD and ODD profiles of the DBDS. The nocturnal enuresis group was more depressed and anxious than the control group according to the results of the CDI and STAI. The mean score of the PHCSC was significantly lower in the nocturnal enuresis group when compared to the normal control group. Conclusion : The results of this study suggest that children with nocturnal enuresis in Korea have clinically relevant behavioral and emotional problems. The findings support the link between nocturnal enuresis and psychopathology in Korean children.

정신분열병에 대한 리스페리돈의 효과 및 안정성

이민수,김용구,김영훈,연병길,오병훈,윤도준,윤진상,이철,정희연,강병조,김광수,김동언,김명정,김상훈,김희철,나철,노승호,민경준,박기창,박두병,백기청,백인호,손봉기,손진욱,양병환,양창국,우행원,이정호,이종범,이홍식,임기영,전태연,정영조,정영철,정인과,정인원,지익성,채정호,한상익,한선호,한진희,서광윤 大韓神經精神醫學會 1998 신경정신의학 Vol.37 No.1

연구목적 : 본 시험의 목적은 임상시험 시작전에 연구자들을 대상으로 PANSS Workshop을 통하여 PANSS, ESRS에 대한 국내에서의 표준화 작업을 구축하고 새로운 정신병 치료제인 리스페리돈의 효과와 안정성을 재확인하여 리스페리돈 사용에 대한 적정화를 이루는데 있다. 연구방법 : 1996년 4월부터 1996년 9월까지 국내 39개 대학병원 정신과에 입원중인 혹은 증상이 악화되어 입원하는 정신분열병 환자 377명을 대상으로 다시설 개방 연구를 시행하였다. 1주일간의 약물 배설기간을 가진후, 리스페리돈을 8주간 투여하였고, 기준점, 1주, 2주, 4주, 그리고 8주후에 평가되었다. 용량은 제1일에는 리스페리돈 1mg씩 1일 2회, 제2일에는 2mg씩 1일 2회, 제3∼7일에는 3mg씩 1일 2회 투여하였다. 이후 환자의 임상상태에 따라 임의로 증량할 수 있으며, 최대 일일 16mg을 초과하지 않도록 하였다. 추체외로 증상을 조절하기 위한 투약을 허용하였다. 임상증상 및 부작용의 평가는 PANSS(Positive and Negative Syndrome Scale), CGI(Clinical Global Impression) 그리고 ESRS(Extrapyramidal Symptom Rating Scale)을 사용하였다. 연구결과 : 377명중 343명(91%)이 8주간의 연구를 완결하였다. 치료 종결시점인 8주후 PANSS 총점수가 20% 이상 호전된 경우를 약물 반응군으로 정의할때, 약물반응군은 81.3%였다. 리스페리돈에 반응하는 예측인자로는 발병연령, 이전의 입원 횟수, 유병기간이 관련 있었다. 리스페리돈은 1주후부터 PANSS양성, 음성, 및 일반정신병리 점수상에 유의한 호전을 보여 효과가 빨랐다. CGI의 경우도 기준점에 비해 1주후부터 유의한 감소를 나타내었다. ESRS의 경우, 파킨슨 평가점수는 기준점과 비교해 투여 1주, 2주, 4주후 유의하게 증가되었다가 8주후 기준점과 차이가 없었다. Dystonia 평가점수는 1주후만 유의한 증가를 보였으며, dyskinesia 평가점수는 유의한 차이가 없었다. 혈압, 맥박수의 생명징후 및 일반 혈액학 검사, 생화학적 검사, 심전도 검사에서 유의한 변화는 없었다. 결 론 : 이상의 다시설 개방 임상 연구를 통해 리스페리돈은 정신분열병 환자에서 양성증상뿐만 아니라 음성증상 및 전반적인 증상에도 효과적인 것으로 사료된다. 보다 명확한 평가를 위해서는 다른 항정신병약물과의 이중맹검 연구가 필요할 것으로 생각되며, 또한 장기적 치료에 대한 평가도 함께 이루어져야 하겠다. Objective : The purpose of this study was to investigate the efficacy and safety of risperidone in the treatment of Korean schizophrenic patients. Method : This multicenter open study included 377 schizophrenic patients drawn from 39 university hospitals. After a wash-out period of 1 week, the schizophrenic patients were treated with risperidone for 8 weeks and evaluated at 5 points ; at baseline, and 1, 2, 4 and 8 weeks of treatment. The dose was increased from 2mg/day(1mg twice daily) to 6mg/day(3mg twice daily) during the first week and adjusted to a maximum of 16mg/day over the next 7 weeks according to the patient's clinical response. Medication to control extrapyramidal symptoms was permitted. The psychiatric and neurological status of the patients was assessed by PANSS, CGI, and ESRS scales. Results : 343(91%) of 377 patients completed the 8-week trial period. Clinical improvement, as defined by a 20% or more reduction in total PANSS score at end point, was shown by 81.3% of patients. The predictors of response to risperidone were associated older age, shorter duration of illness, fewer previous hospitalization. Risperidone had rapid onset of action ; a significant decrease of the total PANSS and three PANSS factor(positive, negative, general), and CGI was already noticed at the end of first week. For the ESRS, parkinsonism rating scores were significantly increased until week 4 comparing with baseline. Dystonia rating scores were significantly increased until week 1, and dyskinesia rating scores were not significantly changed during the study. Laboratory parameters including vital sign, EKG, hematological, and biochemical values showed no significant changes during the trial. Conclusions : This study suggests that risperidone is generally safe and effective against both the positive and negative symptoms in our group of patients.

ADPCM 통신을 위한 ISDN 기본율 접속구조

홍진표,허재헌,권원현 안양대학교 자연과학연구소 1995 自然科學硏究 Vol.2 No.-

비저지 시스템을 강력하게 추진하려는 전송설비와 통신망에 저속 코딩 기술이 출현하고 있다. 본 논문에서는 하나의 국제 표준 ISDN 기본율 접속을 통한 ADPCM 음성전송을 위하여 스위치 액세스 구조를 제안하였다. 제안한 구조에서는 2개, 3개 또는 4개의 음성 터미날과 데이타 터미날이 접속을 통하여 동시에 동작될 수 있다. 다양한 기술적 내용을 논의하였으며 제안한 기본율 접속구조, 기본율 S와 T 접속 비트 프레밍 요구와 Q.931 호 제어와 새로운 구조에 필요한 회선할당 절차를 논의하였다. 연구결과 기존 ISDN 스위치에서 제안한 구조로 갱신하는데 비교적 간단한 하드웨어 추가로 될 수 있고 표준 ISDN S와 T접속은 그대로 유지된다. This paper proposes a switch access architecture for Adaptive Differential Pulse Code Modulation voice transimssion over a single international standard Inetegrated Services Digital Network, Basic Rate Interface. In this proposed architecture, data terminals with two voice terminals, three voice terminals, or four voice terminals can be operated simultaneously through the interface. Various technical topics are dicussed : the proposed Basic Rate Interface architecture. BRI S and T interface bit framing requirements and the Q.931 call control and channel assignment procedures required for the new architecture. The study results show that relatively simple additional hardware could be used to upgrade an existing ISDN switch to this proposed architecture and the standard ISDN S and T interfaces remain intact.

사람 태아 간의 과립백혈구 형성에 관한 전자현미경적 연구

홍진숙,김경용,이원복,김동창 대한해부학회 1992 Anatomy & Cell Biology Vol.25 No.4

혈액 내 구리, 아연 및 ceruloplasmin 농도에 흡연, 음주 및 신체적 활동이 미치는 영향

홍연표,강은용,신인철,최병선,박정덕,장임원,박진완 大韓産業醫學會 1999 대한직업환경의학회지 Vol.11 No.4

Objectives : To investigate the influence of smoking, alcohol ingestion, and physical activity on copper and zinc in RBC and serum and serum ceruloplasmin, this study was performed in a cross-sectional study in 113 healthy men aged 20 to 40 years who had no symptomatic liver, heart, gastrointestinal, and other chronic diseases. Methods : At the men's entry into the study, blood samples were drawn from each subject and immediately centrifuged for analysis of copper, zinc, iron, ceruloplasmin, total cholesterol, and hematocrit. Each man completed a questionnaire that provided information on smoking, amount of alcohol intake, and physical activity. Partial regression analysis was performed on confounding variables such as age, body mass index, hematocrit, serum cholesterol, and serum iron. Results : In general linear models, adjustment for confounding variables did not show statistical differences, and there was only an increasing tendency in serum copper in heavy smoker(P=0.0678). There was no difference between high physical activity with mild smokers and lower physical with heavy smokers. Conclusions : This study suggested that copper, zinc and ceruloplasmin were not good biomarker for early effect by smoking, alcohol intake and physical activity in young adult. However, selection bias should be considered in evaluation of this result, and a large prospective study will be needed in advance on usefulness of copper, zinc and ceruloplasmin as a marker for risk factors and early change of atherosclerosis.