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Lee, Beom Seok,Lee, Yang Ui,Kim, Han-Sang,Kim, Tae-Hyeong,Park, Jiwoon,Lee, Jeong-Gun,Kim, Jintae,Kim, Hanshin,Lee, Wee Gyo,Cho, Yoon-Kyoung Royal Society of Chemistry 2011 Lab on a chip Vol.11 No.1
<P>We report a fully integrated device that can perform both multiple biochemical analysis and sandwich type immunoassay simultaneously on a disc. The whole blood is applied directly to the disposable “lab-on-a-disc” containing different kinds of freeze-dried reagents for the blood chemistry analysis as well as reagents required for the immunoassay. The concentrations of different kinds of analytes are reported within 22 min by simply inserting a disc to a portable device. Using the innovative laser irradiated ferrowax microvalves together with the centrifugal microfluidics, the total process of plasma separation, metering, mixing, incubation, washing, and detection is fully automated. The analyzer is equipped with an optical detection module to measure absorbances at 10 different wavelengths to accommodate the various kinds of reaction protocols. Compared to the conventional blood analysis done in clinical laboratories, it is advantageous for point-of-care applications because it requires a smaller amount of blood (350 μL <I>vs.</I> 3 mL), takes less time (22 min <I>vs.</I> several days), does not require specially trained operators or expensive instruments to run biochemical analysis and immunoassay separately.</P> <P>Graphic Abstract</P><P>We report a fully integrated lab-on-a-disc system for simultaneous analysis of clinical chemistry and enzyme-linked immuno-sorbent assay (ELISA) from whole blood. <IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=c0lc00205d'> </P>
Vancomycin 내성 장구균에 대한 Vancomycin, Penicillin 및 Gentamicin의 병용효과
이위교,이선민,곽연식 대한화학요법학회 1997 대한화학요법학회지 Vol.15 No.2
목적 : VRE의 출현으로 인하여 내성 균주에 의한 감염시 치료 요법 선택에 어려움이 예상되며 여러 약제의 병합 요법이 강구되고 있다. 본 연구의 목적은 VanA, B, 및 C형 내성 균주에 대하여 vancomycin, penicillin 및 gentamicin의 병용에 의한 살균 효과를 알아보고자 하였다. 방법 : VRE 9 균주와 vancomycin 감수성 2균주를 대상으로 하였고 MIC는 한천 희석법을 시행하였다. 시험 약제는 vancomycin 8㎍/mL, penicillin 2㎍/mL, 및 gentamicin 1㎍/mL 였고 병용에 의한 상승 효과는 24시간 후 균수가 2log_(10) CFU/mL 이상 감소시 유효하다고 판정하였다. 결과 : VanA와 VanB 균주는 Vancomycin-penicillin-gentamicin 및 vancomycin-penicillin 병용에 대하여 억제 효과를 보였고 VanC 균주는 어떠한 약제 병용에도 저항성이었다. 결론 : 3 약제 병합 요법과 vancomycin-penicillin 병용 요법은 VanA와 VanB 균주에 치료효과가 기대되며 VanC 균주에 대한 치료 방법에 대해서는 새로운 모색이 필요하다고 사료된다. Background : Because of the resistance that enterococci already exhibit to a 2a riety of antimicrobial agents, the emergence of vancomycin resistance is troublesome. The combined regimen mag offer a treatment for patients infected with vancomycin-resistant enterococci. We examined the antibiotic synergistic killing of clinical isolates of vancomycm-resistant enterococci belonging to genotypic resistance classes A, B, and C. Methods : Nine strains of vancomycin-resistant enterococcl and 2 strains of vancomycin-susceptible enterococci were utilized in this study. MICs were determined by agar dilution method. Synergistic inhibition of growth was assessed by addition vancomycin(8 ㎍/mL), peniciilin( 2 ㎍/mL), gentamicin( 1 ㎍/mL), or combinations of these antibiotics to cultures in the early logarithmic phase of growth. Synergy was defined as a decrease in CFU/mL by at least 2 log10 after 24 h compared with the effect of the most active single agent. Results : Vancomycin-penicillin-gentamicin, and vancomycin-penicillin resulted in 2 or more logs of killing above that the most effective single agent for all VanA and VanB strains. All VanC strains were resistant to any antibiotics combination. Conclusion : Triple combination therapy and vancomycin plus penicillin regimen may be effective for the treatment of infections caused by vancomycin-resistant enterococci belong to genotypic classes VanA and VanB, Futher studies are needed for the new therapeutic regimen agaist VanC strains.
Pseudomonas vesicularis 균혈증의 임상세균학적 고찰 -4예보고-
이위교,이정명,이동화 대한감염학회 1995 감염 Vol.27 No.6
저자들은 면역기전이 저하된 환자와 의료기구 장착 환자에서 P. vesicularis 에 의한 균혈증 4예를 경험하였기에 문헌고찰과 함께 보고하는 바이다. Pseudomonas vesicularisis a motile, nonfermentive gram negative bacillus and belongs to group IV of the genus Pseudomonas. Although P. vesicularis is rarely isolated from environmental sources or clincal specimens, the exact pathogenic role of thisorganism is not confirmed vet. There are rare clinical reports of human infection with this organism in Korea. P. vecicularis produces opportunistic in the immunocompromised host or in patient with foreign bodies. Identification of P. vesicularis has been increasing in frequency, since automated or semiautomated identification system of bacteria was introduced. We report 4 cases P. vesicularis bacteremia with a review of literatures. All patients were immunocompromised hosts such as premature newborn, diabets mellitues, and liver cirrhosis with medical devices were umbilical catheter, urinary catheter, chest tube, and nasogastric tube.
이위교,전희선,곽연식,김종태 아주대학교 의과학연구소 1996 아주의학 Vol.1 No.1
Background: Salmonella infections in human present a spectrum of clinical syndromes that include enteric fever, acute gastroenteritis, bacteremia, localized infections, asymptomatic intestinal infections and transient, convalescent and chronic intestinal carrier states. Recently the incidence of reported nontyphoidal Salmonella cases has increased. Among them, group B Salmonella is an important enteric pathogen and increased incidence of bacteremia in infants is also reported. We performed a retrospective analysis to evaluate the incidence of group B Salmonella infection, the clinical manifestation, the laboratory findings and the pattern of susceptibility to commonly used antibiotics. Methods: The serogroups of Salmonella strains isolated from stool and blood from June 1994 to September 1995 were typed and the antimicrobial susceptibility was tested by agar diffusion method. We reviewed clinical records of patients who had positive culture for group B Salmonella. The age, sex, body temperature, white blood cell count and Widal test results of those patients were analyzed. Results: 1. Among 124 strains, group B Salmonella was 58(48.8%). 2. About 57% of patients were infants and early childhood and most of cases(77.6%) occurred between May and October, 3. Two patients(4.7%) were associated with bacteremia. 4. The resistance to Tetracycline, Ampicillin, Trimethoprim-Sulfamethoxazole (TMP-SXT), cefamandole and ceftriaxone for group B was 62%, 32.7%, 8.6%, 1.7% and 0% respectively. Multiple resistant strains were 7(12.1%). Conclusion: Most patients with acute gastroenteritis due to group B Salmonella are under two years old and the resistant strains to first line antibiotics have increased. Young infants are at particular risk for bacteremia, so if group B Salmonellosis is suspected in infants, blood culture should be obtained and proper antibiotic therapy should be given.