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Phylogeny of the Korean Erwinia Species as Determined by Comparison of 16S rDNA Sequences
( Thapa,S. P. ),( H. R. Park ),( C. K. Lim ),( J. H. Hur ) 강원대학교 농업생명과학연구원(구 농업과학연구소) 2011 강원 농업생명환경연구 Vol.23 No.4
The complete 16S rRNA sequences of the genus Erwinia species isolated from diverse geographical area from Korea were determined following isolation and cloning of the amplified genes. These sequences along with the sequences of other Erwinia species obtained from the GenBank were aligned and phylogenetic trees were inferred by VectorNTI and neighbor-joining method. The levels of sequence similarity between different type strains broadly ranged from 90-100%. Erw. rhapontici and Erw. persicinus showed a distinct seperate cluster from that of amylovora group. The Korean Erw. carotovora subsp. carotovora strains were closer to Asian strains (strains from Japan, China more closer to Chinese strains) than to the European and American strains.
Thapa, P.,Lee, A.R.,Choi, D.H.,Jeong, S.H. Elsevier Sequoia 2017 Powder technology Vol.310 No.-
<P>The main purpose of this study was to investigate the effects of moisture content (MC) of different deformation granules (brittle, plastic, and elastic) and compression pressure (CP) on the physical properties of tablets. A general full factorial design was used with three blocks (deformation property of excipient) and two control factors: MC and CP. Lactose monohydrate (LM), microcrystalline cellulose (MCC), and corn starch (CS) were selected as brittle, plastic, and elastic materials, respectively. The granules were prepared with a high-shear granulator having a capacity of 0.9 L by adding water using a peristaltic pump. This high-shear granulator allows recording of real-time impeller torque values with 1-s intervals. The torque curves can facilitate determination of the transition between saturation stages of a system. Analysis of variance was performed to determine the significance of each factor and their interactions with response variables. All control factors and deformation properties of excipient showed significant effects on the properties of tablets, including ejection force, tensile strength, and porosity (p < 0.05). However, their mutual interaction was not significant on the response variables (p>0.05). Analysis of the relationship between ejection work and peak force (F-max) demonstrated that the ejection work profiles were associated with the deformation properties of the excipient. Moreover, tablet strength was dependent on the control factors and the deformation nature of excipient As these factors significantly influenced the physical properties of tablets, achieving high-quality tablets would require comprehensive information on the excipient properties while considering the compression process.</P>
Process strategy for 2,3-butanediol production in fed-batch culture by acetate addition
Lee, S.J.,Choi, H.S.,Kim, C.K.,Thapa, L.P.,Park, C.,Kim, S.W. Korean Society of Industrial and Engineering Chemi 2017 Journal of industrial and engineering chemistry Vol.56 No.-
<P>Various strategies were studied to enhance 2,3-butanediol production using Enterobacter aerogenes SUMI014. The synergistic effect of acetate improved yield and productivity, and resulted in 32.3% increase in 2,3-butanediol production, compared to the wild strain. Optimizing the fermentation conditions successfully increased the 2,3-butanediol production. In batch fermentation, 93.75 g/L of 2,3-butanediol was obtained within 54 h, along with 0.49 g/g of yield and 1.74 g/L/h of productivity. The highest 2,3-butanediol production achieved was in fed-batch fermentation with acetate addition strategy, with production, yield and productivity of 126.10 g/L, 0.38 g/g and 2.10 g/L/h, respectively. (C) 2017 The Korean Society of Industrial and Engineering Chemistry. Published by Elsevier B.V. All rights reserved.</P>
Thapa, P.,Kadayat, T.M.,Park, S.,Shin, S.,Thapa Magar, T.B.,Bist, G.,Shrestha, A.,Na, Y.,Kwon, Y.,Lee, E.S. Academic Press ; Academic Press 2016 Bioorganic chemistry Vol.66 No.-
<P>A new series of 2-phenol-4-chlorophenyl-6-aryl pyridines were designed, synthesized, and evaluated for topoisomerase (topo) I and II inhibitory activities as well as cytotoxic activity against four different human cancer cell lines such as HCT15, T47D, DU145, and Hela. Most of the tested compounds exhibited stronger topo II inhibitory activity at 100 mu M as compared to etoposide. All the compounds, except 39, did not show topo I inhibitory activity. Interestingly, compounds that showed better topo II inhibition than etoposide have ortho-or para-chlorophenyl at 4-position of central pyridine, and none of the compounds possess meta-chlorophenyl. SAR study revealed the importance of ortho-or para-chlorophenyl at 4-position of the central pyridine for selective topo II inhibitory activity. Similarly, all compounds possessing meta-or para-hydroxyphenyl moieties showed moderate to significant cytotoxic effects. Particularly, compounds 27-37, and 39 which showed excellent cytotoxicity (IC50 = 0.68-1.25 mu M) against T47D breast cancer cells suggest the importance of meta-or para-hydroxyphenyl moiety at 2-position of the central pyridine for the design of anticancer agents with related scaffolds. (C) 2016 Elsevier Inc. All rights reserved.</P>
Identification of a N 7-guanine adduct of 1-bromopropane in calf thymus DNA by mass spectrometry
Thapa, P.,Kim, E. K.,Nepal, M. R.,Jeong, K. S.,Kang, M. J.,Noh, K.,Lee, S.,Jeong, H. G.,Lee, J. H.,Jeong, T. C. THE KOREAN SOCIETY OF TOXICOGENOMICS AND TOXICOPRP 2016 MOLECULAR AND CELLULAR TOXICOLOGY Vol. No.
<P>As a replacement for chlorofluorocarbons that cause ozone depletion, 1-bromopropane has been widely used in work place. In the present study, the formation of N-7-guanine adduct in DNA by 1-bromopropane was evaluated in vitro to elucidate the possible mechanism of its toxic action. N-7-Propyl guanine was chemically synthesized and structurally characterized by NMR, UV, HPLC, and liquid chromatographyelectrospray ionization mass spectrometry (LC- ESI MS) for using as a reference standard. An incubation of 2'-deoxyguanosine with 1-bromopropane produced N-7-propyl adduct, which was identified by UV, HPLC and ESI-MS. In addition, N-7-guanine adduct was also identified from the incorporation of calf thymus DNA with 1-bromopropane at the physiological condition by LC-ESI MS. Furthermore, the production of adduct was proportional to the amounts of 1-bromopropane used. These results indicated that the molecular mechanism underlying toxic effects of 1-bromopropane would be associated with the adduct formation on DNA at least in part.</P>
생산단계 애호박( Cucurbita spp.) 중 boscalid의 잔류 특성
정슬아 ( S. A. Jeong ),박홍열 ( H. R. Park ),한아름 ( A. R. Han ),타파쉬리 ( S. P. Thapa ),최영호 ( Y. H. Choi ),허장현 ( J. H. Hur ) 강원대학교 농업생명과학연구원(구 농업과학연구소) 2012 강원 농업생명환경연구 Vol.24 No.2
This study was carried out to aid an establishment of guideline for safe use of boscalid in squash (Cucurbita spp.). Boscalid was sprayed on squash according to safe use guideline. Samples were collected 2 hr after spray at 1, 3, 5, 7, and 10 days, and was analyzed by GLC/ECD. The limit of detection of boscalid was 0.02 mg/kg. The biological half life of boscalid was found to be 2.5-2.8 days. The residual amount of boscalid 10 days before harvest was 13.68 mg/kg. The residue levels in boscalid was lower than the established MRL.
Comparative study of sediment erosion on alternative designs of Francis runner blade
Bidhan Rajkarnikar,Hari P. Neopane,Biraj S. Thapa 한국유체기계학회 2015 International journal of fluid machinery and syste Vol.8 No.3
The aim of this study was comparative analysis of sediment-induced erosion on optimized design and traditional design of Francis runner blade. The analysis was conducted through laboratory experiments in a test rig called Rotating Disc Apparatus. The results showed that the extent of erosion was significantly less in the optimized design when compared based on the material loss. It was observed that the optimized design could reduce sediment erosion by about 14.4% if it was used in place of the reference design for entire duration of the experiment. Based on the observations and results obtained, it has been concluded that the optimization of hydraulic design of blade profile of Francis runner can significantly reduce the effect of sediment-induced erosion.
Comparative study of sediment erosion on alternative designs of Francis runner blade
Rajkarnikar, Bidhan,Neopane, Hari P.,Thapa, Biraj S. Korean Society for Fluid machinery 2015 International journal of fluid machinery and syste Vol.8 No.3
The aim of this study was comparative analysis of sediment-induced erosion on optimized design and traditional design of Francis runner blade. The analysis was conducted through laboratory experiments in a test rig called Rotating Disc Apparatus. The results showed that the extent of erosion was significantly less in the optimized design when compared based on the material loss. It was observed that the optimized design could reduce sediment erosion by about 14.4% if it was used in place of the reference design for entire duration of the experiment. Based on the observations and results obtained, it has been concluded that the optimization of hydraulic design of blade profile of Francis runner can significantly reduce the effect of sediment-induced erosion.
비름( Amaranthus mangostanus L.) 중 acetamiprid 5% 액제의 잔류특성 및 안전성 평가
박홍열 ( H. R. Park ),허성진 ( S. J. Heo ),타파쉬리 ( S. P. Thapa ),유일호 ( I. H. Yu ),조준모 ( J. M. Cho ),허장현 ( J. H. Hur ) 강원대학교 농업생명과학연구원(구 농업과학연구소) 2012 강원 농업생명환경연구 Vol.24 No.2
This study was investigated to determine the residual amounts of acetamiprid in amaranthus (Amaranthus mangostanus L.) using high performance liquid chromatography with ultraviolet detector (HPLC/UVD) and to assess the standard for safe use of acetamiprid 5% SL. Acetamiprid is used to control the green peach aphid (Myzus persicae) on amaranthus. Recovery tests of acetamiprid in amaranthus were done by spiking 10 and 50-times of limit of detection (LOD). Recoveries obtained from two concentrations were 81.98% and 79.52%, respectively. The residual amount of acetamiprid from the amaranthus harvested 3, 7 and 10 days after 1 and 2-times treatment ranged from 0.06 to 1.63 mg/kg. Because the maxium residue limit (MRL) of acetamiprid in the amaranthus is 3.0 mg/kg (Provisional MRLs, Brassica leafy vegetables, KFDA), it is proposed for acetamiprid 5% SL to apply 2 times, 3 days before harvest in Korea.
Bacterial Conditions of KNU-High School Student`s Household and School Environmental Surfaces
( Jeong In Hur ),( Jong Hoon Choi ),( A R Han ),( S P Thapa ) 강원대학교 농업생명과학연구원(구 농업과학연구소) 2013 강원 농업생명환경연구 Vol.25 No.1
We spend approximately 90% of our life indoors, yet we know little about the source and diversity of microbes in built environments. In the present study, the bacterial contamination of household and school environment surfaces was studied. Total APCs in all the swab samples ranged from geometric mean of 10 to 10 5 CFU/cm2/ml. This study found kitchens to have the greatest degree of bacterial contamination, followed by bath rooms. Toilets had less bacterial contamination than was expected. No significant difference in bacterial load was observed between the house and school environment surfaces. Among the five major pathogens examined, Staphylococcus. aureus was the only pathogen detected. The most dominant bacteria were Staphylococcus, Acinetobacter, Bacillus sp., Corynebacterium, Micorcoccus, Microbacterium, Aquinicola, Brevundimonas, Enterobacter, Kocuria, Leptothrix, Roseomonas, Serratia, Sphingomonas, and Candidawhich are typically associated with human skin and ubiquitous in environment. This study provides a preliminary scenario of bacterial level in home and school environment surfaces.